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A flow injection-fluorometric method for the determination of ammonium in fresh and saline waters with a view to in situ analyses ArchiMer
Aminot, Alain; Kerouel, Roger; Birot, Dominique.
A version of the orthophthaldialdehyde-fluorescence ammonium determination for flow injection analysis (FIA) is presented here, with a view to its use for in situ, low-power consumption systems, Thus, the reaction temperature was limited to 30 degreesC and FIA was used in stop-Bow mode (3 min stop). The calibration is linear up to 50 mu mol l(-1), but concentrations up to 100 mu mol l(-1) can be measured. Repeatability is around 1% in the range of 0.5-4 mu mol l(-1) and the detection limit is about 0.03 mu mol l(-1) Over the salinity range of 5-35 (seawater practical salinity scale) the salt effect is almost negligible (within +/- 2%); and below salinity of 5 it increases to a maximum of -9% in fresh water compared to seawater. Hydrogen carbonate,...
Tipo: Text Palavras-chave: In situ; Fluorometry; Flow injection analysis; Determination; Ammonium.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-1722.pdf
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A re-examination of matrix effects in the segmented-flow analysis of nutrients in sea and estuarine water ArchiMer
Coverly, Stephen; Kerouel, Roger; Aminot, Alain.
Modern instruments together with the use of standard reference materials have improved the accuracy and long-term reproducibility of the analysis of nutrients (ammonium, nitrate, nitrite, phosphate and silicate) in sea water using segmented-flow analysis, so that errors arising from matrix effects become more significant. Colorimetric detectors with bubble-through flowcells have become widely used for seawater analysis in recent years and their associated matrix effects are described. A re-examination of all categories of matrix effects, whose main origin is salinity, was thus undertaken to assess how much they are liable to alter the data. Interferences were classified into four types, each of which was examined in order to show its influence on the...
Tipo: Text Palavras-chave: Nutrient; Determination; Seawater; Estuarine water; Segmented-flow analysis; Matrix effects.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00057/16843/14530.pdf
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Detection and characterization of human rotavirus in hospitalized patients in the cities of Ponta Grossa, Londrina and Assai - PR, Brazil BJID
Nozawa,Carlos M; Kerntopf,Gustavo Franthesco; Czernisz,Érika da Silva; Albuquerque,Daniele; Romanin,Priscila; Freitas,José Felipe Eliseu; Santos,Norma; Benati,Fabrício José; Pietruchinski,Eduardo; Linhares,Rosa Elisa Carvalho.
Acute diarrheal disease is still one of the major public health problems worldwide. Rotaviruses (RV) are the most important viral etiologic agents and children under five years of age are the target population. OBJECTIVE: To investigate the rate of RV infection in hospitalized patients due to acute diarrhea in the cities of Ponta Grossa, Londrina and Assai - Paraná. METHODS: Latex agglutination (LA); immunochromatography (ICG); polyacrylamide gel electrophoresis (PAGE) and negative staining electron microscopy (ME) tests were used to detect the virus. For the genotyping, RT-PCR and RT-PCR-ELISA were used, respectively, for NSP4 and VP4/VP7. RESULT: Out of 124 samples there were 69 positive stool samples for RV, for at least one of the used tests, 67 of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Determination; Hospitalized patients; Genotyping.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600002
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Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca BABT
Morais,Glaucia Almeida de; Takaki,Massanori.
Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Α-amylase; Β-amylase; Determination; Selective inactivation; Phaseolus vulgaris.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100003
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Direct determination of Cu, Mn, Pb, Zn in beer by thermospray flame furnace atomic absorption spectrometry. Repositório Alice
NASCENTES, C. C.; KAMOGAWA, M. Y.; FERNANDES, K. F.; ARRUDA, M. A. Z.; NOGUEIRA, A. R. de A.; NÓBREGA, J..
In this work, thermospray flame furnace atomic absorption spectrometry (TS-FF-AAS) was employed for Cu, Mn, Pb, and Zn determination in beer without any sample digestion. The system was optimized and calibration was based on the analyte addition technique. A sample volume of 300 Al was introduced into the hot Ni tube at a flow-rate of 0.4 ml min1 using 0.14 ml -1 nitric acid solution or air as carrier. Different Brazilian beers were directly analyzed after ultrasonic degasification. Results were compared with those obtained by graphite furnace atomic absorption spectrometry (GFAAS). The detection limits obtained for Cu, Mn, Pb, and Zn in aqueous solution were 2.2, 18, 1.6, and 0.9 ug l-1, respectively. The relative standard deviations varied from 2.7% to...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Determination; Spectrometry.
Ano: 2005 URL: http://www.alice.cnptia.embrapa.br/handle/doc/47179
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Direct determination of metals in bovine seminal plasma. Repositório Alice
RODRIGUES, S. H. B.; CARRILHO, E. N. V.; FERREIRA, R. S. F.; NOGUEIRA, A. R. de A.; BARBOSA, R. T..
2000
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Determination; Metals; Seminal; Plasma; Bovine; Plasma seminal; Bovino; Mental.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/45290
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Easier detection of invertebrate "identification-key characters" with light of different wavelengths ArchiMer
Koken, Marcel; Grall, Jacques.
The marine alpha-taxonomist often encounters two problems. Firstly, the "environmental dirt" that is frequently present on the specimens and secondly the difficulty in distinguishing key-features due to the uniform colours which fixed animals often adopt. Here we show that illuminating animals with deep-blue or ultraviolet light instead of the normal white-light abrogates both difficulties; dirt disappears and important details become clearly visible. This light regime has also two other advantages. It allows easy detection of very small, normally invisible, animals (0.1 mu m range). And as these light wavelengths can induce fluorescence, new identification markers may be discovered by this approach.
Tipo: Text Palavras-chave: Fluorescent colours; Light emission; Determination; Cryptic species; Dimorphism; Pattern UV light; Deep-blue light; Autofluorescence.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00185/29660/28026.pdf
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Génétique des huîtres ArchiMer
Gras, Marie-paule; Gras, Paul.
Following the high mortality which killed the Portuguese oysters Crassostrea angulata cultivated on the French coasts, the massive establishment of Pacific oysters Crassostrea gigas, resisting the disease and the creation of artificial deposits for the latter was decided. In most culture areas, the mortality was estimated at more than 80 % of the stock, but, in some regions, some small populations of Crassostrea angulata subsisted and kept on reproducing. A genetic control of the spawner stocks had to be made. The first step was to know if the oysters living in the same areas were of the same species in order to foresee the next generations and try to improve them (selection).
Tipo: Text Palavras-chave: Sélection; Determination; Crassostrea gigas; Crassostea angulata; Huîtres; Génétique.
Ano: 1977 URL: http://archimer.ifremer.fr/doc/1977/acte-6579.pdf
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Méthode de dosage du cadmium, du cuivre, du plomb et du zinc dans la chair de poissons ArchiMer
Auger, Dominique.
An analytical technique for cadmium, copper, lead and zinc determination in fish tissue used in monitoring programmes is described. Atomic absorption spectrophotometry on nitric digested samples is performed.
Tipo: Text Palavras-chave: Cadmium; Cuivre; Poisson; Plomb; Zinc; Analyse; Cadmium; Copper; Lead; Zinc; Fish; Determination.
Ano: 1989 URL: https://archimer.ifremer.fr/doc/00489/60059/63343.pdf
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Sequential Extraction of Cr, Cu anel V in Soil anel Determination Using ICP·MS. Repositório Alice
NOGUEIRA, A. R. de A.; BIANCHI, S. R.; NÓBREGA, J. A..
Among the instrumental techniques that can be useel for eletermination of trace elements in soils inductively coupled plaSl1la mass spectroscopy (ICP-MS) presents aelvantages such as multielemental character and superb sensitivity. However, aspects such as the content of total e1issolveel solids anel the polyatomic íons formeel in complex matrices may limit its application. The determination of Cr. Cu and V may suffer from polyatomic intelierences either originated in the argon plasma 01' in the matrix. In the case of 65Cu+ the main interferents are 23Na40Ar+ anel 23Na40Ca+ The eletermination of 52Cr+ is affecteel by 40Ar12C+ and 51V+ is affected by 35Cl16O+. Notwithstanding, all these elements also have less abundant isotopes not affected by severe...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sequential Extraction; Soil; Determination; ICP·MS.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/574496
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Stability of the anthocyanins extracted from residues of the wine industry Ciênc. Tecnol. Aliment.
Clemente,Edmar; Galli,Dirseu.
Anthocyanins are highly important due to their antioxidant capacity. They are the most important among the phenolic compounds and one of the main natural dyes used in the food industry. In this research, residue of processed grapes was used to investigate the presence of anthocyanins, the possibility of their extraction from the residue, and their stability. The extraction solution consisted of 70 mL of ethanol 70% and 30 mL of HCl 0.1% at pH 2.0. The results found for the processed grapes residue was 26.20 mg.100 g-1. In order to evaluate stability, caffeic acid was added at 0.5:1 w/v; 0.8:1 w/v; and 1:1 w/v concentrations. Anthocyanins extract reached the greatest stability at 0.5:1 w/v concentration, with 82.47% color retention and a half-life period of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthocyanins; Co-pigment; Determination; Stability.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000300033
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The determination of total dissolved free primary amines in seawater: Critical factors, optimized procedure and artefact correction ArchiMer
Aminot, Alain; Kerouel, Roger.
The paper reviews the methods described in the literature for the determination of total dissolved free primary amines (TDFPA) by fluorescence. A wide set of reaction conditions can be found, but they rely on few experiments for their validation. Among fluorogenic compounds, o-plithaldialdehyde (OPA) is more sensitive than fluorescamine and was thus examined here. However, the use of mercaptoethanol (ME) in the reaction (as ail additional derivatization compound) is able to generate unreliable results, in particular when standardization relies on glycine. We suggest replacing ME with 3-mercaptopropionic acid (MPA) which induces more stable and comparable fluorescence among amine Compounds. A systematic study was therefore undertaken to define reagent...
Tipo: Text Palavras-chave: Fluorescence; Determination; Seawater; Total dissolved free primary amines.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2274.pdf
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The Karyotype of the parasitoid Chelonus insularis cresson (Hymenoptera, braconidae, cheloninae). Repositório Alice
SILVA-JUNIOR, J.C.; POMPOLO, S. das G.; CAMPOS, L.A. de O.; CRUZ, I..
The Karyotype of chelonus insularis (Hymenoptera, Braconidae Cheloninae) is described. The males show an haploid number of seven chromosomes and the females a diploid number of fourteen chromosomes, confirming haplo-diploid sex determination. Comparisons of these results with karyotypes of other species of the same family were done and a possible mechanism involved in the karyotype evolution of this species is discussed.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Citogenetica; Sexo; Determinacao; Cromossomo; Cytogenetic; Sex; Determination; Chromosome.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484457
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Voltammetric characteristics of miconazole and its cathodic stripping voltammetric determination Anais da ABC (AABC)
PEREIRA,FRANCISCO C.; STRADIOTTO,NELSON R.; ZANONI,MARIA VALNICE B..
Miconazole is reduced at mercury electrode above pH 6 involving organometallic compound formation, responsible for an anomalous polarographic behavior. The electrodic process presents a large contribution of the adsorption effects. The drug can be determined by cathodic stripping voltammetry from 8.0 x 10-8 to 1, 5 x 10-6 molL-1 in Britton-Robinson buffer pH 8.0, when pre-accumulated for 30s at an accumulation potential of 0V. A relative standard deviation of 3.8% was obtained for ten measurements of 1.0 x 10-7 molL-1 miconazole in B-R buffer pH 8.0 and a limit detection of 1, 7 x 10-8 molL-1 was determined using 60s of deposition time and scan rate of 100 mVs-1. The proposed method is simple, precise and it was applied successfully for the determination...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Miconazole; Voltammetry; Electroanalysis; Determination.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000300005
Registros recuperados: 14
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