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COLNACOQ. Composés lipophiles naturels dans l’environnement des coquillages. Rapport final ArchiMer
Hess, Philipp; Geiger, Marie; Brochard, Solène; Lepretre, Thomas; Fessard, Valerie; Antignac, Jean-philippe; Dupont, Jacques; Sechet, Veronique; Vanel, Faustine; Mondeguer, Florence; Herve, Fabienne; Leborgne, Sabrina; Deslanglois, Gwenaëlle; Marshall, Lindsey; Rounds, Lucy; Guitton, Yann; Amzil, Zouher; Grovel, Olivier; Ruiz, Nicolas; Pouchus, Francois.
Cette étude s’est inscrite dans un contexte d’évaluation de la salubrité des mollusques bivalves destinés à la consommation humaine. Ces organismes peuvent en effet accumuler des toxines, en particulier de microalgues, en concentrations importantes, pouvant induire des intoxications chez les personnes les consommant. Afin d’assurer la protection des consommateurs, les toxines réglementées sont recherchées dans les coquillages par techniques physico-chimiques. En parallèle, les toxines émergentes, potentiellement produites par d’autres organismes que les micro-algues telles que les micromycètes, peuvent être detectées par l’utilisation du test de toxicité aigüe sur souris. Cependant, ce bio-essai présente de très nombreux inconvénients, notamment éthiques...
Tipo: Text Palavras-chave: Cytotoxicité; Toxicité sur larves de diptères; Activité antibactérienne; Bactéries marines; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00253/36470/35015.pdf
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Écophysiologie des dinoflagellés du genre Azadinium, production toxinique et transfert trophique vers les mollusques bivalves ArchiMer
Jauffrais, Thierry.
This study has been conducted in order to develop the analysis of AZAs and to produce AZAs from A. spinosum culture. It also aimed at studying the effect of environmental and nutritional factors on growth and toxin production. The study also demonstrated a link between A. spinosum and the accumulation of AZAs in shellfish, followed by the clarification of the processes of accumulation, detoxification and biotransformation of AZAs into mussels. A quantitative analysis of AZAs in A. spinosum cultures was developed, and the formation and structure of the AZA methylated analogues was explained and minimised. This work also demonstrated the feasibility of a sustainable production of AZAs from A. spinosum culture and highlighted the main factors influencing...
Tipo: Text Palavras-chave: Azadinium spinosum; Mytilus edulis; Azaspiracides; Phycotoxines; Dinoflagellés; Écophysiologie; CL-SM/SM; Azadinium spinosum; Mytilus edulis; Azaspiracids; Phycotoxins; Dinoflagellate; Ecophysiology; LC-MS/MS.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00111/22217/19891.pdf
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Effect of the PSP-causing dinoflagellate, Alexandrium sp. on the initial feeding response of Crassostrea gigas ArchiMer
Wildish, David; Lassus, Patrick; Martin, Jennifer; Saulnier, Alinne; Bardouil, Michele.
The effects of toxic and non-toxic strains of Alexandrium sp. on the initial feeding responses of the Pacific oyster Crassostrea gigas were determined by continuous monitoring of the clearance rate of individual oysters. This was determined fluorometrically by loss of fluorescence of live microalgae introduced as food. Feeding oysters with toxic or non-toxic A. tamarense and toxic A. fundyense resulted in stop/start clearance behaviour. In contrast, control oysters fed a reference microalga, Isochrysis sp., known to support their growth, did not exhibit this behavior. Pacific oysters acclimated to Isochrysis sp., fed mixtures of Alexandrium/Isochrysis, showed further evidence of stop/start clearance. Exposure for periods up to 48 hours with non-toxic A....
Tipo: Text Palavras-chave: Crassostrea gigas; Physiologie alimentaire; Microalgues toxiques; Dinoflagellés; Paralytic shellfish poisoning; Crassostrea gigas; Feeding physiology; Toxic microalgae; Dinoflagellate; Paralytic shellfish poisoning.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/1998/publication-877.pdf
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Évaluation d’une suite de bio-essais pour la détection et l’étude de composés lipophiles de micro-organismes marins issus de mollusques bivalves et de leur environnement ArchiMer
Geiger, Marie.
The context of this study was the assessment of the safety of edible bivalve molluscs. Bivalve can indeed accumulate high concentrations of phycotoxins into their hepatopancreas, potentially leading to human intoxication outbreaks after consumption of such contaminated bivalves. In order to ensure the protection of shellfish consumers, regulated toxins are monitored using physico-chemical techniques. In parallel, emerging toxins, that can be putatively produced by microalgae or marine fungi, are monitored using the intraperitoneal mouse bioassay. However, this bioassay suffers ethical and methodological drawbacks. Thus, the objective of this work was to assess the ability of a bioassay suite to detect lipophilic toxins. This suite was composed of three...
Tipo: Text Palavras-chave: Cytotoxicité; Larves de diptères; Activité antibactérienne; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii; Cytotoxicity; Diptera larvae; Antibacterial activity; Lipophilic toxins; Microalgae; Dinoflagellates; Marine fungi; Penicillium; Okadaic acid; Azaspiracid; Pinnatoxin G; Vulcanodinium rugosum; Azadinium spinosum; Prorocentrum lima; Beauveria brongniartii.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00183/29426/27787.pdf
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Modélisation des blooms de Gymnodinium mikimotoï le long de la façade atlantique française : répartition spatiale ArchiMer
Loyer, Sophie; Lazure, Pascal; Gentien, Patrick; Menesguen, Alain.
In order to study the occurrence of Gymnodinium mikimotoï bloom on the French Atlantic coast, a three-dimensional model of primary production was set up. The biological model was coupled to a hydrodynamic model previously developed at Ifremer, by the intermediary of transport, diffusion and heat fluxes. The cycles of three limiting elements for the phytoplankton growth were modelised: nitrogen, phosphorus and silicon. The second phase of the study aimed at reproducing the summer dinoflagellate blooms. In this new approach a model of the species Gymnodinium mikimotoï was coupled with the previous biomass model which provides the physical and chemical environments. The study focuses on the results obtained by this specific sub-model. The parametrisation was...
Tipo: Text Palavras-chave: Turbulence; Dinoflagellés; Gymnodinium mikimotoï; Modèle; Nutriments; Turbulence; Dinoflagellate; Gymnodinium mikimotoï; Model; Nutrients.
Ano: 2001 URL: https://archimer.ifremer.fr/doc/00479/59100/61738.pdf
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Modélisation du devenir à moyen terme dans l'eau et le sédiment des éléments majeurs (N, P, Si) rejetés par la Seine en Baie de Seine ArchiMer
Cugier, Philippe.
The Bay of Seine is a coastal ecosystem submitted to a large river input (Seine river), which brings important quantities of nutrients. This nutrient enrichment leads to frequent eutrophication events and, from the beginning of the eighties, regular toxic phytoplanktonic (Dynophysis) blooms are observed. In order to study the nutrient cycles (nitrogen, phosphorus, silica) and their contribution to phytoplanktonic stock regulation, a three dimensional model has been developed, based on the coupling between an hydrodynamic part taking into account sediment dynamics (SAM-3D) and an ecological part, which accounts for the main biological compartments and processes. This model is able to reproduce the main physical and biological characteristics of the...
Tipo: Text Palavras-chave: Bay of Seine (France); Dinoflagellates; Nutrients; Nutrients; Eutrophication; Sediments; Hydrodynamic; Mathematical models; Baie de (France); Seine; Dinoflagellés; Diatomées; Nutriments; Eutrophisation; Sédiments (géologie); Hydrodynamique; Modèles mathématiques.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/these-3220.pdf
Registros recuperados: 6
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