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| Rushdy,Abeer Ahmed; Mabrouk,Mona Ibrahim; Abu-Sef,Ferialla Abdel-Hamid; Kheiralla,Zeinab Hassan; Abdel -All,Said Mohamed; Saleh,Neveen Mohamed. |
| OBJECTIVES: To study the potential factors include gene mutation, efflux pump and alteration of permeability associated with quinolone-resistance of Salmonella enterica strains isolated from patients with acute gastroenteritis and to evaluate the degree of synergistic activity of efflux pump inhibitors when combined with ciprofloxacin against resistant isolates. METHODS: Antimicrobial resistance patterns of fifty-eight Salmonella isolates were tested. Five isolates were selected to study the mechanism of resistance associated with quinolone group, including mutation in topoisomerase-encoding gene, altered cell permeability, and expression of an active efflux system. In addition, the combination between antibiotics and efflux pump inhibitors to overcome the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Fluoroquinolone resistance; Efflux pump; Outer membrane protein; Chromosomal mutations. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000400008 |
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| Dalmolin,Tanise Vendruscolo; Bianchini,Bianca Vendruscolo; Rossi,Grazielle Guidolin; Ramos,Ana Carolina; Gales,Ana Cristina; Trindade,Priscila de Arruda; Campos,Marli Matiko Anraku de. |
| Abstract Carbapenems are considered last-line agents for the treatment of serious infections caused by Klebsiella pneumoniae, and this microorganism may exhibit resistance to β-lactam antibiotics due to different mechanisms of resistance. We evaluated 27 isolates of K. pneumoniae resistant to carbapenems recovered from inpatients at the University Hospital of Santa Maria-RS from July 2013 to August 2014. We carried out antimicrobial susceptibility, carbapenemase detection, testing for the presence of efflux pump by broth microdilution and loss of porin by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Genetic similarity was evaluated by ERIC-PCR. High levels of resistance were verified by the minimum inhibitory concentration for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Klebsiella pneumoniae; Carbapenemases; Loss of porins; Efflux pump. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300493 |
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| Ferrari,Rafaela Gomes; Galiana,Antonio; Cremades,Rosa; Rodríguez,Juan Carlos; Magnani,Marciane; Tognim,Maria Cristina Bronharo; Oliveira,Tereza C.R.M.; Royo,Gloria. |
| Several studies have been conducted in recent years to elucidate the structure, function and significance of AcrB, MarA, SoxS and RamA in Salmonella enterica. In this study, the relative quantification of acrB, soxS, marA and ramA genes expression was evaluated in 14 strains of S. enterica, with or without accompanying mutations in the quinolone resistance-determining regions of the gyrA gene, that were exposed to ciprofloxacin during the exponential growth phase. The presence of ciprofloxacin during the log phase of bacterial growth activated the genes marA, soxS, ramA and acrB in all S. enterica strains analyzed in this study. The highest expression levels for acrB were observed in strains with gyrA mutation, and marA showed the highest expression in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella sp.; Fluoroquinolone; Efflux pump; Antibiotic resistance. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200002 |
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| Gressler,Letícia Trevisan; Vargas,Agueda Castagna de; Costa,Mateus Matiuzzi da; Pötter,Luciana; Silveira,Bibiana Petri da; Sangioni,Luis Antônio; Botton,Sônia de Avila. |
| The req_39680 gene, associated to a putative efflux system, was detected in 60% (54/90) of R. equi isolates by PCR. The phenotypic expression of efflux mechanism was verified in 20% of the isolates using ethidium bromide. For the first time, the expression of efflux mechanism was demonstrated in R. equi. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ethidium bromide; Efflux pump; Multidrug resistance; Rhodococcus equi; VapA. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200040 |
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