SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Strabelli,Tânia Mara Varejão; Cais,Daiane P.; Zeigler,Rogério; Siciliano,Rinaldo; Rodrigues,Cristhieni; Carrara,Dirceu; Neres,Suzi; Lessa,Sarita; Uip,David Everson.

Early identification of an outbreak is one of the main advantages of routine epidemiological surveillance. Enterococcus spp. used to be regarded as microorganisms of low pathogenicity, because they are part of the normal microbial flora of the gastrointestinal and genitourinary tract. Recently, they have emerged as important pathogenic agents, sometimes causing infections with high mortality rates. We studied a clustering of primary bloodstream infections caused by Enterococcus faecalis in a cardiology hospital neonatal intensive care unit (NICU). Four cases of primary bloodstream infection by E. faecalis were detected from April 15 to May 13, 2004, during active infection surveillance. The isolates were sensitive to glycopeptides. Some aspects of the...

Enterococcus faecalis resistant to vancomycin and teicoplanin (VanA phenotype) isolated from a bone marrow transplanted patient in Brazil

Palavras-chave: Enterococcus faecalis; Clustering; Neonatal intensive care unit; Bloodstream; Infection.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000200008

Cereda,Rosangela F.; Sader,Helio S.; Jones,Ronald N.; Sejas,Lilian; Machado,Antônia M.; Zanatta,Yara P.; Rego,Sinaida T. M. S.; Medeiros,Eduardo A. S..

We report for the first time in Brazil, a patient from whom an Enterococcus faecalis VanA phenotype was isolated. Glycopeptide resistance is not commonly observed in Enterococcus faecalis, so this finding is of great concern since this species is responsible for 90% of enterococcal infections in Brazil. The isolate was recovered from a surveillance rectal swab culture from a patient with acute lymphocytic leukemia (ALL). Identification to the species level was performed by conventional biochemical tests and Vitek GPI cards. Antimicrobial susceptibility testing was evaluated by use of broth microdilution and Etest (AB BIODISK, Solna, Sweden) methods. The isolate was identified as E. faecalis and was considered resistant to both vancomycin (MIC, > 256...

Tipo: Info:eu-repo/semantics/report

Palavras-chave: Enterococcus faecalis; Glycopeptide resistance; VanA; Initial case report; Transplant.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000100007

First report of vancomycin-resistant Enterococcus faecalis in Uberaba, Minas Gerais state

Conceição,Natália; Darini,Ana Lúcia da Costa; Palazzo,Izabel Cristina Vanzato; Oliveira,Cristina da Cunha Hueb Barata de; Oliveira,Adriana Gonçalves de.

In this study we report the first isolation of VanA-type vancomycin-resistant Enterococcus faecalis strains from two different patients hospitalized in the same intensive care unit at the hospital of Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, Minas Gerais, Brazil.

In vitro activity of Amazon plant extracts against Enterococcus faecalis

Palavras-chave: Vancomycin resistance; Enterococcus faecalis; VanA genotyping.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100007

Castilho,Adriana Lígia de; Silva,Juliana Paola Correa da; Saraceni,Cintia Helena Coury; Díaz,Ingrit Elida Collantes; Paciencia,Mateus Luís Barradas; Varella,Antonio Drauzio; Suffredini,Ivana Barbosa.

Previous studies analyzing 2,200 plant extracts indicated anti-enterococcal activity in 25 extracts obtained from Brazilian forests' plants. In the present study, these extracts were subjected to microdilution broth assay (MDBA) and disk diffusion assay (DDA) using planktonic Enterococcus faecalis ATCC® 29212TM and were submitted to phytochemical analysis in TLC and HPLC. Three extracts obtained from Ipomoea alba (MIC < 40 µg/mL), Diclinanona calycina (MIC < 40 µg/mL) and Moronobea coccinea (40 < MIC < 80 µg/mL; MBC = 80 µg/mL) showed significant bactericidal activity in the MDBA and four extracts obtained from I. alba (14.04 ± 0.55 mm diameter) S. globulifera (14.43 ± 0.33 mm and 12.18 ± 0.28 mm diameter) and Connarus ruber var. ruber (13.13 ±...

In vitro fosfomycin activity in vancomycin-resistant Enterococcus faecalis

Palavras-chave: Enterococcus faecalis; Endodontitis; Antibacterial activity; Amazon rain forest; Bioprospection.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300002

Superti,Silvana; Dias,Cícero Armídio Gomes; d'Azevedo,Pedro Alves.

Enterococci are part of the endogenous flora of human beings, are naturally resistant to several classes of antimicrobials, and are able to acquire resistance with relative ease. Currently the vancomycin-resistant enterococci are spread all over the world and treatment options for infections caused by it are often extremely limited. We assessed 193 vancomycin-resistant Enterococcus faecalis isolates collected from four different hospitals in Porto Alegre for their susceptibility to fosfomycin using the E-test and agar diffusion. Fosfomycin proved to be active in vitro against the great majority of isolates, indicating that it is a valid option in the treatment of these infections.

Incidence of virulence determinants in clinical Enterococcus faecalis and Enterococcus faecium isolates collected in Bulgaria

Palavras-chave: Enterococcus faecalis; Vancomycin-resistance; VRE; Fosfomycin.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000200010

Strateva,Tanya; Atanasova,Daniela; Savov,Encho; Petrova,Guergana; Mitov,Ivan.

Abstract Objectives To evaluate the prevalence of some virulence genes among 510 clinical Enterococcus spp. isolates and to assess the association of those genes with the species, infection site, and patient group (inpatients/outpatients). Methods Adhesins genes (aggregation substances agg and asa1 of Enterococcus faecalis and Enterococcus faecium, respectively), enterococcal surface protein (esp), endocarditis-specific antigen A (efaA), collagen-binding proteins (ace/acm)); invasins (hyaluronidase (hyl) and gelatinase (gelE)); cytotoxines (activation of cytolysin (cylA) in E. faecalis); and modulators of the host immunity and inflammation (enhanced expression pheromone (eep) in E. faecalis) were detected by polymerase chain reaction. Results The...

Screening of enzymatic activities for the depolymerisation of the marine bacterial exopolysaccharide HE800

Palavras-chave: Enterococcus faecalis; Enterococcus faecium; Virulence genes; Prevalence.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000200127

ArchiMer

Rigouin, Coraline; Delbarre-ladrat, Christine; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, Michel.

Tipo: Text

Palavras-chave: Polysaccharide; Enzymatic depolymerisation; Low-molecular-weight derivatives; Enterococcus faecalis; Endo-N-acetyl-galactosaminidase.

Ano: 2012

URL: http://archimer.ifremer.fr/doc/00098/20907/18741.pdf

Soy Protein Isolate-Alginate Microspheres for Encapsulation ofEnterococcus faecalis HZNU P2

BABT

Zhang,Yu; Zheng,Wei; Gu,Jian-Fei; Ni,Jian; Wang,Ling; Tang,Zhen-Xing; Shi,Lu-E.

ABSTRACTIn this work, the mixture of alginate and soy protein isolate used as a wall material was developed to encapsulateEnterococcus faecalis HZNU P2 (E. faecalis HZNU P2). The survival ability in the simulated gastric fluid (SGF) and bile salt solution, storage stability at different temperatures and release properties in the simulated intestinal fluid (SIF) of encapsulated cells were assessed. The results showed that encapsulation could offer sufficient protection toE. faecalis HZNU P2. The viability of encapsulatedE. faecalis HZNU P2 did not decrease in SGF at pH 2.5 or 2.0 after 2 h incubation, while free cells were reduced from 11 to 9.85 log CFU/mL in SGF (pH 2.5) at the same exposure time. Only minor viability of encapsulatedE. faecalis HZNU P2...

Study on the susceptibility of Enterococcus faecalis from infectious processes to ciprofloxacin and vancomycin

Palavras-chave: Enterococcus faecalis; Encapsulation; Soy protein isolate; Alginate; Stability; Viability.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500805

J. Venom. Anim. Toxins incl. Trop. Dis.

Genaro,A.; Cunha,M. L. R. S.; Lopes,C. A. M..

Enterococcus faecalis is considered a pathogen responsible for hospital infections and, due to its frequent multi-resistant profile, has caused preoccupations among many medical authorities. The objective of this study was to determine the antimicrobial susceptibility of 74 strains isolated from blood cultures and purulent secretions to vancomycin and ciprofloxacin through the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by using the Microdilution test. The results showed a greater efficacy of vancomycin compared to ciprofloxacin (98.6% of the strains were inhibited by vancomycin at lower concentrations: 0.06 - 1 µg/ml). However, in the MBC analysis 73% of the strains showed a MBC of vancomycin only at high...

Virulence factors in vancomycin-resistant and vancomycin - susceptible Enterococcus faecalis from Brazil

Palavras-chave: Enterococcus faecalis; Vancomycin; Ciprofloxacin; Resistance; Spontaneous mutation.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300004

Camargo,I. L. B. C.; Zanella,R. C.; Gilmore,M. S.; Darini,A. L. C..

Enterococci are members of commensal flora of animals and insects, but are also important opportunistic pathogens. Our objective was to observe if there was any difference of virulence in several groups of E. faecalis, mainly between vancomycin-resistant E. faecalis (VREFS) of colonization and infection. VREFS and vancomycin-sensitive E. faecalis from Brazil were screened for the presence of virulence factor genes. Phenotypic assays were used to assess in vitro expression, to understand the pathogenic potential of these isolates and to determine whether a correlation exists between virulence and antibiotic resistance. Different virulence profiles were found suggesting that the disseminating clone may have generated several variations. However, our study...

Virulence-associated characteristics of Enterococcus faecalis strains isolated from clinical sources

Palavras-chave: Enterococcus faecalis; VRE; Esp; Gelatinase; Aggregation substance.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200014

Furumura,Márcia T.; Figueiredo,Patricia M.S.; Carbonell,Gleize V.; Darini,Ana Lucia da Costa; Yano,Tomomasa.

Thirty-two clinical isolates of Enterococcus faecalis were screened for virulence factors. Twenty-four (75%) isolates produced hemolysin on Mueller-Hinton blood agar plates with sheep erythrocytes. However, the cell free heat-stable hemolysin was detected in all isolates (100%) of E. faecalis when grown in BHI-GA (BHI medium supplemented with 1% glucose and 0.03% L-arginine), but not in BHI broth alone. Twenty-four isolates (75%) produced caseinase and 23 (71.9%) lipase, but none of the isolates produced gelatinase. Fifteen (46.9%) culture filtrates caused rounding and membrane alterations with blebbing formation followed by death in HeLa and HEp-2 cells, but not in Vero cells. Thirteen isolates (40.6%) agglutinated rabbit erythrocytes, but did not produce...