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| Registros recuperados: 12 | |
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| Nascimento,Maristela da Silva do; Moreno,Izildinha; Kuaye,Arnaldo Yoshiteru. |
| This study investigated the antimicrobial activity of Enterococcus faecium FAIR-E 198 against Bacillus cereus, Listeria monocytogenes and Staphylococcus aureus. Using the critical-dilution method, the bacteriocin produced by E. faecium FAIR-E 198 inhibited all L. monocytogenes strains evaluated (1,600 to 19,200 AU mL-1). However, none of the B. cereus and S. aureus strains investigated were inhibited. The maximum activity of this bacteriocin (800 AU mL-1) was observed in MRS broth, while the activity in milk was 100 AU mL-1. In the co-cultivation test in milk, B. cereus K1-B041 was reduced to below the detection limit (1.00 log CFU mL-1) after 48 h. E. faecium reduced the initial L. monocytogenes Scott A population by 1 log CFU mL-1 after 3 h at 35ºC.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococcus faecium; Bacteriocin; Biopreservation; Gram-positive pathogen. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100013 |
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| Alves,Geraldo da Silva; Pereira,Monalessa Fábia; Bride,Lais de Lima; Nunes,Ana Paula Ferreira; Schuenck,Ricardo Pinto. |
| ABSTRACT Vancomycin-resistant Enterococcus faecium (VREfm) has emerged as an important global nosocomial pathogen, and this trend is associated with the spread of high-risk clones. Here, we determined the genetic and phenotypic features of 93 VREfm isolates that were obtained from patients in 13 hospitals in Vitória, Espírito Santo, Brazil, during 2012-2013. All the isolates were vancomycin-resistant and harbored the vanA gene. Only 6 (6.5%) of the VREfm isolates showed the ability to form biofilm. The 93 isolates analyzed belong to a single pulsed-field gel electrophoresis lineage and presented six subtypes. MLST genotyping showed that all VREfm belonged to ST412 (the high-risk clone, hospital-adapted). The present study describes the dissemination of... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Enterococcus faecium; VRE; ST412; Biofilm. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600656 |
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| Moemen,Dalia; Tawfeek,Doaa; Badawy,Wafaa. |
| Vancomycin resistant Enterococcus faecium (VREF) ia an emerging and challenging nosocomial pathogen. This study aimed to determine the prevalence, risk factors and clonal relationships between different VREF isolates in the intensive care units (ICUs) of the university hospitals in our geographic location. This prospective study was conducted from July, 2012 until September, 2013 on 781 patients who were admitted to the ICUs of the Mansoura University Hospitals (MUHs), and fulfilled the healthcare-associated infection (HAI) criteria. Susceptibility testing was determined using the disk diffusion method. The clonal relationships were evaluated with pulsed field gel electrophoresis (PFGE). Out of 52 E. faecium isolates, 12 (23.1%) were vancomycin resistant.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococcus faecium; ICU; Vancomycin; Risk factors; PFGE. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300777 |
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| Strateva,Tanya; Atanasova,Daniela; Savov,Encho; Petrova,Guergana; Mitov,Ivan. |
| Abstract Objectives To evaluate the prevalence of some virulence genes among 510 clinical Enterococcus spp. isolates and to assess the association of those genes with the species, infection site, and patient group (inpatients/outpatients). Methods Adhesins genes (aggregation substances agg and asa1 of Enterococcus faecalis and Enterococcus faecium, respectively), enterococcal surface protein (esp), endocarditis-specific antigen A (efaA), collagen-binding proteins (ace/acm)); invasins (hyaluronidase (hyl) and gelatinase (gelE)); cytotoxines (activation of cytolysin (cylA) in E. faecalis); and modulators of the host immunity and inflammation (enhanced expression pheromone (eep) in E. faecalis) were detected by polymerase chain reaction. Results The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococcus faecalis; Enterococcus faecium; Virulence genes; Prevalence. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000200127 |
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| Santos,Karina Maria Olbrich dos; Vieira,Antônio Diogo Silva; Salles,Hévila Oliveira; Oliveira,Jacqueline da Silva; Rocha,Cíntia Renata Costa; Borges,Maria de Fátima; Bruno,Laura Maria; Franco,Bernadette Dora Gombossy de Melo; Todorov,Svetoslav Dimitrov. |
| This study aimed to characterize the safety and technological properties of Enterococcus faecium strains isolated from Brazilian Coalho cheeses. High levels of co-aggregation were observed between Enterococcus faecium strains EM485 and EM925 and both Escherichia coli and Clostridium perfringens. Both strains presented low levels of hydrophobicity. E. faecium EM485 and EM925 were both able to grow in the presence of 0.5% of the sodium salts of taurocholic acid (TC), taurodeoxycholic acid (TDC), glycocholic acid (GC), and glycodeoxycholic acid (GDC), although they showed the ability to deconjugate only GDC and TDC. Both strains showed good survival when exposed to conditions simulating the gastro intestinal tract (GIT). When tested for the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococcus faecium; Probiotic properties; Technological properties; Virulence factors; Antibiotic resistance. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100237 |
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| Bedendo,J.; Pignatari,A.C.C.. |
| Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC strains, representative of 11 species of Enterococcus, were initially tested by JB1-PCR, revealing that Enterococcus malodoratus and Enterococcus hirae presented identical banding patterns. Eight Enterococcus faecium isolates from Stanford University and 12 from São Paulo Hospital were studied by JB1-PCR, REP-PCR 1/2R and PFGE. Among the isolates from Stanford University, 5 genotypes were defined by JB1-PCR, 7 by REP-PCR 1/2R and 4 by PFGE. Among the isolates from São Paulo Hospital, 9 genotypes were identified by JB1-PCR, 6 by REP-PCR and 5 by PFGE. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular typing; Enterococcus faecium; PCR-PFGE. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100002 |
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| Loddi,Maria Marta; Gonzales,Elisabeth; Takita,Tânia Sayuri; Mendes,Ariel Antônio; Roça,Roberto de Oliveira. |
| Dois mil e quatrocentos pintos de corte foram distribuídos em um delineamento inteiramente ao acaso, com esquema fatorial 2 x 2 x 2 (sexo, com e sem probiótico, com e sem antibiótico), totalizando oito tratamentos e seis repetições com 50 aves cada uma. Independente da suplementação recebida, os machos sempre foram mais pesados que as fêmeas. Entretanto, a análise de desempenho apresentou maiores peso corporal e ganho de peso para os machos que receberam suplementação de antibiótico, quando comparados aos não-suplementados. Até 42 dias de idade, o uso do antibiótico determinou aumento do consumo de ração, mas não influiu nas demais características de desempenho. No período de 1 a 21 dias e 1 a 42 dias de idade, a suplementação com probiótico influenciou... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Análise sensorial; Avoparcina; Carcaça; Enterococcus faecium; Frango de corte; Rendimento. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982000000400025 |
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| Kerbauy,G.; Perugini,M.R.E.; Yamauchi,L.M.; Yamada-Ogatta,S.F.. |
| In this study, we report the characterization of a strain of Enterococcus faecium vanA, which grows only in the presence of vancomycin (VDEfm-UEL). The bacterium was isolated from the feces of a female patient who had undergone surgical treatment of Reinke’s edema and was receiving intravenous vancomycin therapy for infection with methicillin/oxacillin-resistant Staphylococcus aureus, a postoperative complication. Antimicrobial dependence was further confirmed by the vancomycin E-test. VDEfm-UEL was also shown to be resistant to ampicillin, ciprofloxacin, chloramphenicol, erythromycin, levofloxacin, penicillin, rifampicin, and teicoplanin. The putative virulence genes efaA, gelE and esp were detected by PCR. The ddl gene from VDEfm-UEL was cloned and... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Enterococcus faecium; Vancomycin; Dependence; VanA genotype; Virulence factors. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300013 |
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| Registros recuperados: 12 | |
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