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| Dorneles,Athos Odin Severo; Pereira,Aline Soares; Sasso,Victória Martini; Possebom,Gessieli; Tarouco,Camila Peligrinotti; Schorr,Márcio Renan Weber; Rossato,Liana; Ferreira,Paulo Ademar Avela; Tabaldi,Luciane Almeri. |
| ABSTRACT Potato cultivation is widespread around the world, being exposed to several abiotic stresses, including soils with high aluminum (Al) availability. Silicon (Si) is recognized for alleviating the stress caused by Al in various plant species. Thus, the aim of this study was to investigate the potential of Si to mitigate the oxidative stress caused by Al in potato genotypes, exhibiting differential sensitivity toward this element. Plants of the Al-sensitive genotype (SMIJ319-7) and Al-tolerant genotype (SMIF212-3) were grown for two weeks in a hydroponic system with the nutrient solution containing combinations of Al (0 and 1.85 mM) and Si (0, 0.5 and 1.0 mM). At the end of the experiment, photosynthetic parameters, pigment content, root and shoot... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; Beneficial elements; Enzyme activity; Oxidative stress; Toxic elements. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000100012 |
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| Abrameto1,MA; Pozzo Ardizzi,CM; Gil,MI; Molina,LM. |
| Costly investments in storage and transport of onion bulbs makes it increasingly important to identify cultivars with the best chance of long-term storage. This paper discusses our own and other researchers' results and laboratory procedures on onion's pungency, soluble solids, dry matter, respiration rate, carbohydrates and catabolism-related enzymes under different storage conditions. The variability in parameters such as carbohydrate composition, pungency, soluble solids and soluble uronic acid concentration in water is growth dependent, and defines the quality of onion bulbs at harvest time. During storage, these parameters can be modified by environmental conditions, presence of pathogens or termination of dormancy. Under postharvest conditions,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium cepa L.; Respiration rate; Sprouting; Ethylene; Non-structural carbohydrates; Enzyme activity. |
| Ano: 2010 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200003 |
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| SHAN, A. yu K. V.; OLIVEIRA, L. E. M. de; BONOME, L. T. da S.; MESQUITA, A. C.. |
| O objetivo deste trabalho foi avaliar a assimilação metabólica de nitrogênio em plântulas de seringueira cultivadas na presença de nitrato ou de amônio, por meio da quantificação da atividade das enzimas redutase do nitrato (RN), glutamina sintetase (GS), glutamato sintase (GOGAT) e glutamato desidrogenase (GDH). Os seguintes tratamentos foram avaliados: fontes de N ? controle, sem aplicação de N; amônio a 8 mmol L‑1; e nitrato a 8 mmol L‑1 ? e tempos de exposição ? 0 e 27 dias ?, em arranjo fatorial 3x2, no delineamento inteiramente casualizado, com quatro repetições. A forma amoniacal promoveu maior assimilação metabólica de N, o que resultou em maior acúmulo de aminoácidos. A assimilação, tanto da forma nítrica quanto da amoniacal,... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Metabolismo nitrogenado; Plântula; Seringueira; Nitrato; Amonia; Hevea brasiliensis; Enzyme activity; Nitrogen; Metabolism; Nitrates. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/930334 |
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| FONSECA, L. M.; ELOY, A. M. X.; PINHEIRO, R. R.; PINHEIRO, A. A.. |
| Na fase crônica da artrite encefalite caprina (CAE), as metaloproteinases de matriz (MMPs) são ativadas, por desequilíbrios homeostáticos no animal. Elas atuam na degradação e remodelamento extracelular, ocorrendo tanto em processos fisiológicos como patologias de caráter agudo ou crônico. As gelatinases (MMP-2 e MMP-9), em suas formas latente e ativa, terão foco neste trabalho. O objetivo foi identificar a atividade das metaloproteinases -2 e -9, através da Zimografia, no plasma sanguíneo (PS) de caprinos infectados com CAE. |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Zimografia; Artrite encefalite caprina; Matrix metalloproteínases; Metaloproteinase; MMPs; Zymography; Caprino; Atividade Enzimática; Lentivirus; Goat diseases; Goats; Animal diseases; Caprine arthritis-encephalitis; Enzyme activity; Brazil; Metalloproteinases. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120491 |
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| Silva,Patrícia Pereira da; Sekita,Marcelo Coelho; Dias,Denise Cunha Fernandes dos Santos; Nascimento,Warley Marcos. |
| ABSTRACT In carrot seed production, harvesting of the umbels is generally staggered, or sometimes the whole plant is harvested with the umbels at different stages of maturation; in that case, there is a risk of harvesting immature seeds and of losses due to natural dehiscent, or to physiological deterioration as a result of unfavourable environmental factors. The present study aimed to evaluate the physiological and biochemical changes related to the maturation of 'Planalto' carrot seeds from diferent umbels. The samples were collected 120 days after anthesis, with the primary, secondary and tertiary umbels being harvested separately. Seeds were analysed for germination, first count and germination rate, at temperatures of 25 ºC (optimum) to 35 ºC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Daucus carota L.; Physiological quality; Enzyme activity. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902016000200407 |
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| Yap,MKK; Tan,NH; Fung,SY. |
| The lethal and enzymatic activities of venom from Naja sumatrana (Equatorial spitting cobra) were determined and compared to venoms from three other Southeast Asian cobras (Naja sputatrix, Naja siamensis and Naja kaouthia). All four venoms exhibited the common characteristic enzymatic activities of Asiatic cobra venoms: low protease, phosphodiesterase, alkaline phosphomonoesterase and L-amino acid oxidase activities, moderately high acetylcholinesterase and hyaluronidase activities and high phospholipase A2. Fractionation of N. sumatrana venom by Resource® S cation exchange chromatography (GE Healthcare, USA) yielded nine major protein peaks, with all except the acidic protein peak being lethal to mice. Most of the protein peaks exhibit enzymatic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Naja; Cobra venom; Enzyme activity; Cardiotoxin. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400012 |
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| PEIL,GREICE H.S.; KUSS,ANELISE V.; RAVE,ANDRÉS F.G.; VILLARREAL,JOSÉ P.V.; HERNANDES,YOHANA M.L.; NASCENTE,PATRÍCIA S.. |
| ABSTRACT The lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. The aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in Pelotas, RS/Brazil. Bacteria were identified using Gram stain and biochemical tests (Vitek 2(r)). Fungi were identified according to macro and micromorphology characteristics. The extracellular lipase production was evaluated using the Rhodamine B test and the enzymatic activity by titration. Twenty-one bacteria were isolated and identified as Klebsiella pneumoniae ssp. pneumoniae, Serratia marcescens, Enterobacter aerogenes, Raoultella ornithinolytica and Raoultella planticola. Were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Effluent; Enzyme activity; Extracellular lipase production; Lipase enzyme; Lipolytic microorganism. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501769 |
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| Bordon,Karla C. F.; Wiezel,Gisele A.; Cabral,Hamilton; Arantes,Eliane C.. |
| BackgroundCrotalus durissus terrificus venom (CdtV) is one of the most studied snake venoms in Brazil. Despite presenting several well known proteins, its L-amino acid oxidase (LAAO) has not been studied previously. This study aimed to isolate, characterize and evaluate the enzyme stability of bordonein-L, an LAAO from CdtV.Methods The enzyme was isolated through cation exchange, gel filtration and affinity chromatography, followed by a reversed-phase fast protein liquid chromatography to confirm its purity. Subsequently, its N-terminal amino acid sequence was determined by Edman degradation. The enzyme activity and stability were evaluated by a microplate colorimetric assay and the molecular mass was estimated by SDS-PAGE using periodic acid-Schiff... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalus durissus terrificus; L-amino acid oxidase; Rattlesnake; Enzyme activity; Enzyme stability; Chromatography; Snake venom; Yellow venom; Stabilization. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100335 |
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| DORNELAS, J. C. M.; FIGUEIREDO, J. E. F.; ABREU, C. S. de; LANA, U. G. P.; OLIVEIRA, C. A.; MARRIEL, I. E.. |
| Secondary metabolites produced by Actinobacteria of tropical soils represent a largely understudied source of novel molecules with relevant application in medicine, pharmaceutical and food industries, agriculture, and environmental bioremediation. The present study aimed to characterize sixty-nine Actinobacteria isolated from compost and tropical soils using morphological, biochemical, and molecular methods. All the isolates showed high variation for morphological traits considering the color of pigments of the aerial and vegetative mycelium and spore chain morphology. The enzymatic activity of amylase, cellulase, and lipase was highly variable. The amylase activity was detected in 53 (76.81%) isolates. Eighteen (Streptomyces curacoi) and ACSL 6 (S.... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Morphological traits; Identificação molecular; Actinomiceto; Atividade enzimática; Actinobacteria; Enzyme activity; Molecular identification. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076062 |
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| BOGO, A.; COMPARIN, C. C.; SANHIEZA, R. M. V.; RITSCHEL, P. S.; CASA, R. T.; SILVA, F. N.; EVERHART, S. E.. |
| Abstract:The causal agents of apple bull?s-eye rot in southern Brazil have recently been described as Neofabraea actinidiae and N. brasiliensis. Isolates of both species were evaluated for response of mycelial growth index (MGI) to different temperatures, enzyme production, mycelial growth inhibition and effective concentrations (EC 50 and EC 100 ) of the fungicides tri fl umizole, pyrimethanil and thiophanate methyl, as well as aggres- siveness on fruits of ? Fuji ? hybrid and ? Pink Lady ? . There was signi ficantly lower mycelium growth in N. brasiliensis compared with N. actinidiae at all temperatures tested. Neither species grew at 3 and 32°C. There were minor differences in production of enzymes in the two species, with all N. brasiliensis isolates... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Fungicide effective concentration; Mycelium growth rate; Temperature sensitivity; Malus domestica; Enzyme activity. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1088472 |
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| MACEDO,Adrielle Souza Leão; ROCHA,Fátima de Souza; RIBEIRO,Margareth da Silva; SOARES,Sergio Eduardo; BISPO,Eliete da Silva. |
| Abstract The reactions leading to the formation of precursors of chocolate flavor are performed by endogenous enzymes present in the cocoa seed. Polyphenol oxidase (PPO) presence and activity during fermentation of cocoa beans is responsible for the development of flavor precursors and is also implicated in the reduction of bitterness and astringency. However, the reliability of cocoa enzyme activities is complicated due to variations in different genotypes, geographical origins and methods of fermentation. In addition, there is still a lack of systematic studies comparing different cocoa cultivars. So, the present study was designed to characterize the activity of PPO in the pulp and seeds of two cocoa cultivars, PH 16 and TSH 1188. The PPO activity was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cocoa; Fermentation; Enzyme activity; Chocolate. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000100056 |
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