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| Registros recuperados: 10 | |
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| Wang,Hui; Liu,Zheng; Zhang,Wenxiu; Yuan,Ziao; Yuan,Hongyi; Liu,Xueting; Yang,Chunwen; Guan,Weijun. |
| BACKGROUND: Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues. RESULTS: We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd²+ exposure. Apoptosis was dose- (0-4.8 μΜ) and duration-dependent (12-48 h), and proliferation was strongly inhibited. Cd²+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress and mitochondrial dysfunction. It... |
| Tipo: Journal article |
Palavras-chave: Siberian tiger; Cadmium; Apoptosis; Fibroblast; Calcium. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100042 |
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| Sandoval,Catherine; Nuñez,Marcelo; Roa,Ignacio. |
| The need to identify bodies that are found as a result of disappearances with a diversity of causes, illegal burials and massive disasters, represent a wide percentage of dentistry practice on forensic research. The following study determined the performance of Barr Body Test, in fibroblasts of healthy teeth, under different conditions of burial (in vitro) with variations in pH, humidity and salinity in terms of general accuracy and sensitivity for men and women. Analyzed sample considered 47 dental pulps, taken from teeth under burial conditions during a period of a month. From dental pulps samples, 265 histological cuts valid for this study, were obtained, which were observed with an optical microscope under conventional H/E staining. Results showed a... |
| Tipo: Journal article |
Palavras-chave: Barr body; Fibroblast; Dental pulp; Sex identification; Burial conditions. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200026 |
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| Zhang,W.-H.; Li,X.-L.; Guo,Y.; Zhang,Y.. |
| The aim of this study was to determine the proliferation and osteogenic activity of fibroblasts induced with fibronectin and their possible dose-dependent relationship. The fibroblasts obtained by tissue explants adherent method were induced with fibronectin at different concentrations of 0, 10, 20, 40, 60, and 80 μg/mL for 14 days. The 3H-thymidine and 3H-proline incorporation test was used to evaluate the synthesis of DNA and collagen by fibroblasts, respectively. The mineralized nodules and osteocalcin secretion, as vital osteogenic indicators, were detected with tetracycline labeling and 125I-labeled competitive immunoassay, respectively. Fibronectin significantly increased the synthesis of DNA and collagen by fibroblasts, especially at the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibroblast; Fibronectin; Proliferation; Osteogenesis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000604 |
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| Silvestre,J.G.; Baptista,I.L.; Silva,W.J.; Cruz,A.; Silva,M.T.; Miyabara,E.H.; Labeit,S.; Moriscot,A.S.. |
| Fibroblasts are a highly heterogeneous population of cells, being found in a large number of different tissues. These cells produce the extracellular matrix, which is essential to preserve structural integrity of connective tissues. Fibroblasts are frequently engaged in migration and remodeling, exerting traction forces in the extracellular matrix, which is crucial for matrix deposition and wound healing. In addition, previous studies performed on primary myoblasts suggest that the E3 ligase MuRF2 might function as a cytoskeleton adaptor. Here, we hypothesized that MuRF2 also plays a functional role in skeletal muscle fibroblasts. We found that skeletal muscle fibroblasts express MuRF2 and its siRNA knock-down promoted decreased fibroblast migration, cell... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibroblast; Skeletal muscle; Migration; MuRF2; E3 ligase; Wound healing. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900604 |
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| Del Valle,Paulo Roberto; Milani,Cintia; Brentani,Maria Mitzi; Katayama,Maria Lucia Hirata; Lyra,Eduardo Carneiro de; Carraro,Dirce Maria; Brentani,Helena; Puga,Renato; Lima,Leandro A.; Rozenchan,Patricia Bortman; Nunes,Bárbara dos Santos; Góes,João Carlos Guedes Sampaio; Folgueira,Maria Aparecida Azevedo Koike. |
| Cancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR < 0.01). Functional analysis revealed that genes related to development and morphogenesis were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bone marrow; Breast neoplasm; Fibroblast; Gene expression profiling; Lymph node; Mesenchymal stromal cells; Tumor microenvironment. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400002 |
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| Machado,Alessandra Cury; Sartori,Patrícia Fernandes; Damante,Carla Andreotti; Dokkedal,Anne Lígia; Oliveira,Rodrigo Cardoso de. |
| ABSTRACT The aim of this study was to evaluate the effect of ethanolic "aroeira" (Myracrodruon urundeuva) extract on the viability of human gingival fibroblast. For this, fibroblasts (2x103 cells/well) were plated in a 96-well plate and incubated for 24 h; the medium (Eagle's medium modified by Dulbecco - DMEM) supplemented with 10% fetal bovine serum was replaced by DMEM with different ethanolic extract concentration (0, 0.1, 1, 10, 100, and 1000μg / mL). The fibroblast viability was analyzed after 48,72, and 96 h by the neutral red capture test and violet crystal. The "aroeira" extract, at high concentrations (100 and 1000 µg/mL) caused decrease in both cellular viability tests (p<0.05). However, dilutions between 0.1 and 10 µg/mL did not affect the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Medicinal Plant; Fibroblast; Myracrodruon urundeuva; "aroeira"; "aroeira-do-sertão". |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100301 |
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| Registros recuperados: 10 | |
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