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Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines Genet. Mol. Biol.
Garcia,Antonio A. F.; Benchimol,Luciana L.; Barbosa,Antônia M. M.; Geraldi,Isaias O.; Souza Jr.,Cláudio L.; Souza,Anete P. de.
In order to compare their relative efficiencies as markers and to find the most suitable marker for maize diversity studies we evaluated 18 inbred tropical maize lines using a number of different loci as markers. The loci used were: 774 amplified fragment length polymorphisms (AFLPs); 262 random amplified polymorphic DNAs (RAPDs); 185 restriction fragment length polymorphisms (RFLPs); and 68 simple sequence repeats (SSR). For estimating genetic distance the AFLP and RFLP markers gave the most correlated results, with a correlation coefficient of r = 0.87. Bootstrap analysis were used to evaluate the number of loci for the markers and the coefficients of variation (CV) revealed a skewed distribution. The dominant markers (AFLP and RAPD) had small CV values...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diversity studies; Genetic distances; Maize; Molecular markers.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400019
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Diversidad genética y patrón de cruzamiento en poblaciones naturales de Pseudotsuga menziesii (Mirb.) Franco Colegio de Postgraduados
Cruz Nicolas, Jorge.
Se determinó la estructura de la diversidad genética y el patrón de cruzamiento en poblaciones naturales de Pseudotsuga menziesii (Mirb.) Franco en México con base en información de isoenzimas. Para evaluar la diversidad genética se utilizó una muestra de 11 poblaciones naturales distribuidas en diferentes regiones geográficas (noroeste, noreste y centro) del país; el patrón de cruzamiento y su efecto sobre el nivel de endogamia sólo se evaluó en una muestra de tres poblaciones, representativas de cada región geográfica. Se encontró una amplia diversidad genética a nivel de especie (83.3% de loci polimórficos y un promedio de 2.9 alelos por locus), pero reducida a nivel de poblaciones (28.3 % de loci polimórficos y 1.52 alelos por locus), especialmente en...
Tipo: Tesis Palavras-chave: Abeto Douglas; Distancias genéticas; Diversidad genética; Endogamia; Isoenzimas; Polimorfismo; Polinización cruzada; Maestría; Genética; Cross-pollination; Douglas-fir; Genetic distances; Genetic diversity; Inbreeding; Isozymes; Polymorphism.
Ano: 2007 URL: http://hdl.handle.net/10521/1587
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Evaluation of the genetic diversity among maize lines (Zea mays L.) by DNA fingerprinting analysis Ciência Rural
Echeverrigaray,Sergio; Delamare,Ana Paula Longaray; Tosello,Geraldo; Gal,Orit; Hillel,Jossi; Lavi,Uri.
Informativo DNA fingerprint profiles of eight homozygotic maize lines were obtained by the electrophoretic separation of DNA restriction fragments and the ir hybridization with the minisatellite probe R18.1. The analysis of the bandsharing frequencies allowed to identify all the lines and to estimate the genetic distances between them. The relationship obtained by DNA fingerprinting analysis of the eight inbreed lines was highly consistem with the ir genetical origin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular identification; Genetic distances; Zea mays.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000300028
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Genetic distance and its association with heterosis in cacao BABT
Dias,Luiz Antônio dos Santos; Marita,Jane; Cruz,Cosme Damião; Barros,Everaldo Gonçalves de; Salomão,Tânia Maria Fernandes.
The efficiency of cacao breeding program can be increased by choosing superior crosses to be made between divergent clones. We assessed the genetic distance among five clones with RAPD data (genetic distance - GD) and with yield component data (Mahalanobis distance - MD). The clones were evaluated in a diallel, during five years, for five yield components. A total of 130 RAPD bands were scored. GD and MD were used to determine the correlation between genetic distances among clones and the performance of their hybrids. The correlation between GD and MD was 0.67 (P=0.03). Both distances were related to heterotic performance of hybrids for wet seed weight/plant and wet seed weight/fruit. The average hybrid performance for the same two yield components was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic distances; Heterosis; Performance data; RAPD data; Theobroma cacao L..
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300005
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Genetic variability and pedigree analysis of Brazilian common bean elite genotypes Scientia Agricola
Alzate-Marin,Ana Lilia; Costa,Márcia Regina; Sartorato,Aloísio; Del Peloso,María José; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves.
Genetic diversity is essential for any breeding program. However, breeders tend to concentrate on specific genotypes, which combine traits of interest and may be used as progenitors in several breeding programs. Common bean (Phaseolus vulgaris L.) breeding programs are not different in this sense. In this study, the genetic diversity of 21 common bean elite lines from the Bean Regional Trials conducted by the Embrapa Rice and Bean Research Center was evaluated using the Random Amplified Polymorphic DNA (RAPD) and pedigree analyses. Based on genetic dissimilarity, three groups were defined: group I - lines 1, 9 and 10, with low genetic distances among them (0.00 to 0.06), originated from 11 Mesoamerican parents; group II - 17 lines with genetic distances...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Andean cultivars; Mesoamerican cultivars; RAPD molecular markers; Genetic distances.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000200012
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Hypothesis testing of genetic similarity based on RAPD data using Mantel tests and model matrices Genet. Mol. Biol.
Rodrigues,Flávia Melo; Diniz-Filho,José Alexandre Felizola; Bataus,Luiz Artur Mendes; Bastos,Rogério Pereira.
Clustering and ordination procedures in multivariate analyses have been widely used to describe patterns of genetic distances. However, in some cases, such as when dealing with Jaccard coefficients based on RAPD data, these techniques may fail to represent genetic distances because of the high dimensionality of the genetic distances caused by stochastic variation in DNA fragments among the units analyzed (species or populations). In this note, we show how Mantel tests can be used to test hypotheses about genetic distances and avoid problems with multivariate data representation. The procedure is illustrated with RAPD data comparing 20 anuran species from the cerrado, in which Jaccard genetic distances were compared to three model matrices linking the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anura; Clustering; Genetic distances; Mantel test; RAPD.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400013
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Mantel test in population genetics Genet. Mol. Biol.
Diniz-Filho,José Alexandre F.; Soares,Thannya N.; Lima,Jacqueline S.; Dobrovolski,Ricardo; Landeiro,Victor Lemes; Telles,Mariana Pires de Campos; Rangel,Thiago F.; Bini,Luis Mauricio.
The comparison of genetic divergence or genetic distances, estimated by pairwise F ST and related statistics, with geographical distances by Mantel test is one of the most popular approaches to evaluate spatial processes driving population structure. There have been, however, recent criticisms and discussions on the statistical performance of the Mantel test. Simultaneously, alternative frameworks for data analyses are being proposed. Here, we review the Mantel test and its variations, including Mantel correlograms and partial correlations and regressions. For illustrative purposes, we studied spatial genetic divergence among 25 populations of Dipteryx alata ("Baru"), a tree species endemic to the Cerrado, the Brazilian savannas, based on 8 microsatellite...
Tipo: Info:eu-repo/semantics/article Palavras-chave: "Baru" tree; Genetic distances; Geographical genetics; Partial correlation; Partial regression.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400002
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Molecular phylogenetics and comparative anatomy of Kimberleytrachia Köhler, 2011 - a genus of land snail endemic to the coastal Kimberley, Western Australia with description of new taxa (Gastropoda, Camaenidae) Naturalis
Criscione, F.; Köhler, F..
Kimberleytrachia Köhler, 2011 is a genus of camaenid land snail endemic to the Western Australian Kimberley region. It comprises twelve previously recognised species, all of which occur within the high precipitation zone along the north-western coast between the Admiralty Gulf and King Sound and within less than about 50 km distance from the coast. By evaluating the variation in shell and genital anatomy as well as the differentiation in the mitochondrial markers 16S and COI, we assess the monophyly of Kimberleytrachia with respect to other camaenid genera from north-western Australia. In addition, we newly describe six species (K. jacksonensis n. sp., K. leopardus n. sp., K. nelsonensis n. sp., K. serrata n. sp., K. setosa n. sp. and K. silvaepluvialis n....
Tipo: Article / Letter to the editor Palavras-chave: Genetic distances; Helicoidea; Mitochondrial DNA; New species; Stylommatophora; 42.73; 42.64.
Ano: 2014 URL: http://www.repository.naturalis.nl/record/506810
Registros recuperados: 8
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