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| Registros recuperados: 291 | |
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| Martinati,Juliana Camargo; Pacheco,Flávia Tereza Hansen; Miranda,Vitor Fernandes Oliveira de; Tsai,Siu Mui. |
| Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S-23S rDNA (coding for a small subunit ribosomal RNA) spacer region (ITS). Current methods for sequencing the ITS region yields partial sequences that do not contribute with significant information. According to this fact, new primers have been designed in order to obtain a complete sequence and facilitate the sequencing. The complete 16S-23S sequences from 08 strains were amplified through PCR using the primers designed in our lab. The 16S-23S sequences obtained were compared with 52 others sequences entries in GenBank database. The results revealed a higher level of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; 16S-23S rDNA; Intergenic spacer; Identification; Detection. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100033 |
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| Siqueira,Marcos Vinicius Bohrer Monteiro; Borges,Aline; Valle,Teresa Losada; Veasey,Elizabeth Ann. |
| This study was carried out in order to assess the genetic diversity of 20 cassava (Manihot esculenta Crantz) genotypes with high phenotypic performance using microsatellite markers. Two groups were considered for this study: eleven varieties for industrial uses and nine for household consumption. By using nine microsatellite primers, high polymorphism was identified in all the loci analyzed, with values reaching 100%. On average, 3.4 alleles per locus were found, with 0.371 the value estimated for the observed heterozygosity and 0.555 for gene diversity for the entire set of varieties. The genetic variability found in both varieties, cultivated on a large-scale in the South Center region of Brazil, is wide enough to allow the choice of divergent parental... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta; Genetic diversity; Germplasm; Microsatellites. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052011000400003 |
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| Gomes,Regina Lucia Ferreira; Costa,Marcones Ferreira; Alves-Pereira,Alessandro; Bajay,Miklos Maximiliano; Viana,João Paulo Gomes; Valente,Sérgio Emílio dos Santos; Lopes,Ângela Celis de Almeida; Zucchi,Maria Imaculada; Pinheiro,José Baldin. |
| ABSTRACT Some germplasm collections have a high number of accessions, which makes it difficult to explore the genetic variability present in the germplasm bank due to the redundancy and the difficulty of detailed analysis of all conserved accessions. Therefore, our study aimed to analyze the genetic diversity of 153 lima bean (Phaseolus lunatus) accessions for the purpose of constructing a core collection. Eleven SSRs were used for this purpose. The 153 lima bean accessions can be represented by low redundancy using a minimum of 34 accessions, thus representing 22 % of the size of the entire germplasm bank. The core collection had a higher Shannon diversity index and expected heterozygosity (1.906 and 0.811, respectively) than those presented by the entire... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Germplasm; Genetic diversity; Genetic structure; Microsatellite markers. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201100 |
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| Souza,Laís Bérgamo de; Ruas,Eduardo A.; Rodrigues,Luana A.; Ruas,Claudete F.; Ruas,Paulo M.. |
| Parapiptadenia rigida is a tropical early secondary succession tree characteristic of the Tropical Atlantic Rainforest. This species is of great ecological importance in the recovery of degraded areas. In this study we investigated the variability and population genetic structure of eight populations of P. rigida. Five AFLP primer combinations were used in a sample of 159 individuals representing these eight populations, rendering a total of 126 polymorphic fragments. The averages of percentage of polymorphic loci, gene diversity, and Shannon index were 60.45%, 0.217, and 0.322, respectively. A significant correlation between the population genetic variability and the population sizes was observed. The genetic variability within populations (72.20%) was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tropical tree; Genetic diversity; Population genetics; Conservation; AFLP. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400012 |
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| Moura,Tânia Maria de; Sebbenn,Alexandre Magno; Martins,Karina; Moreno,Maria Andreia; Oliveira,Giancarlo Conde Xavier; Chaves,Lázaro José; Kageyama,Paulo Yoshio. |
| This study aimed to compare the genetic diversity of populations of Solanum lycocarpum A.St.-Hil between natural and human disturbed environments, with the assumption that protected areas have greater genetic diversity than disturbed areas. For this study, two populations were sampled in Goiás State, Brazil. One was located in a conservation unit, Serra de Caldas Novas State Park, in the Caldas Novas municipality. The other was located in a pasture area in the municipality of Morrinhos. The two populations are 41 km apart. We sampled 60 individuals from each population, which were genotyped with five microsatellite loci (SSR). The highest number of alleles was recorded in the population of the conservation unit, where we found 11 exclusive and five rare... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerrado; Gene flow; Conservation genetics; Genetic diversity. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062011000400023 |
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| Glasenapp,Jacqueline Siqueira; Barbosa,Priscila Barros; Casali,Vicente Wagner Dias; Martins,Ernane Ronie; Cruz,Cosme Damião. |
| Leaves and fruits from 63 Stryphnodendron adstringens trees were sampled in the Rio Preto State Park to analyze allozyme segregation, tissue specific expression of allozyme loci, and their genetic parameters. The enzyme systems ADH, EST, ACP, PGM, PGI, GDH, G6PDH, GOT, IDH, LAP, MDH, PER and SKDH were assessed by means of starch-gel electrophoresis. The polymorphic systems PGI, IDH, MDH and GOT demonstrated a dimeric quaternary structure, while EST and PER were monomeric. The total expected genetic diversity (H E) for leaves and seeds were 0.325 and 0.244 respectively. The effective number of alleles per locus (A E) was 1.58 in leaves and 1.42 in seeds. The values of H E and A E observed in S. adstringens were comparatively higher than the average values... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allozyme segregation; Genetic diversity; Heterozygosity; Medicinal plants. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042012000200003 |
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| Nagella,Praveen; Hosakatte,Niranjana Murthy; Ravishankar,K.V.; Hahn,Eun-Joo; Paek,Kee-Yoeup. |
| Randomly amplified polymorphic DNA (RAPD) markers were used to estimate genetic diversity among 18 cultivars of niger from India. Total genomic DNA was extracted and subjected to RAPD analysis using 80 arbitrary 10-mer primers; 17 primers were selected, which yielded a total of 124 bands, 41.20% of them polymorphic. None of the primers produced unique banding pattern for each cultivar. RAPD data were used to calculate a Squared-Euclidean Distance matrix which revealed a minimum genetic distance between cultivars JNC-6 and N-48 and a maximum distance between IGP-76 and JN-30. Based on the distance matrix, a cluster analysis was done using a minimum variance algorithm. The dendrogram generated, based on Ward’s method, grouped 18 niger cultivars... |
| Tipo: Journal article |
Palavras-chave: Genetic diversity; Guizotia abyssinica; Molecular markers; RAPD. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100014 |
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| Lirón,J.P.; Ripoli,M.V.; De Luca,J.C.; Peral-García,P.; Giovambattista,G.. |
| Data from five protein-coding loci related to dairy production were used to study the genetic diversity and population structure of Argentine and Bolivian Creole cattle breeds. Genomic DNA was extracted from blood samples of six Creole cattle breeds: Argentine (n = 230), Patagonian (n = 25); "Saavedreño" (n = 140), "Chaqueño Boliviano" (n = 30), "Yacumeño" (n = 27), and "Chusco" (n = 11). kappa-casein, beta-lactoglobulin, growth hormone and prolactin were measured by PCR-RFLP, while alphaS1-casein was typed by PCR-ASO. The results are discussed, focusing on: historical origin, recent differentiation and selection events, Zebu gene introgression, and population structure. This work shows that: (i) For the studied genes, the observed gene frequency profiles... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Creole cattle; Polymorphism; Genetic diversity; Molecular markers. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400010 |
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| Yue,Qingchun; Zhang,Chenfei; Wang,Qinghao; Wang,Wenjing; Wang,Jinyang; Wu,Yueyan. |
| ABSTRACT: The objective of this study is to research the genetic diversity of the ‘ Zuijinxiang ’ grape and its mutant breeding F1 plants, we screened the excellent mutant plants with potential breeding value. 50 mutated single plants obtained from 137Cs-γ irradiated ‘Zuijinxiang’ grape seeds were used as research objects, and SCoT molecular marker technology was used for genetic diversity and variation analysis, and clustering research was carried out. The results showed that: (1) 36 SCoT primers produced abundant polymorphisms, and the amplification results showed obvious bright bands, and the amplification efficiency and polymorphism rate were 100%. (2) A total of 221 bands were amplified by 36 primers, of which 175 were rich in polymorphism, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Grape; SCoT markers; Genetic diversity; Cluster analysis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001100406 |
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| Melo,Daniela Benevides; Menezes,Ana Paula de Oliveira; Reis,Joice Neves; Guimarães,Alaíse Gil. |
| Antibiotic resistance has increased in recent years, raising the concern of public health authorities. We conducted a study of Escherichia coli isolates obtained from human and food samples to assess the prevalence of antimicrobial resistance and to determine the genotype and clonal relationship of 84 E. coli isolates (48 from humans and 36 from foods). An antimicrobial susceptibility test was performed using the disk diffusion method. Virulence factors were evaluated by multiplex PCR, and the clonal relationship among the resistant isolates was studied by Pulsed Field Gel Electrophoresis (PFGE). All isolates were susceptible to ceftriaxone. Overall, 26%, 20.2%, 15.4% and 6% of the isolates were resistant to tetracycline, ampicillin,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Antimicrobial resistance; Food contamination; Genetic diversity. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401165 |
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| Veasey,Elizabeth Ann; Cardin,Daruska; Silva,Rainério Meireles; Bressan,Eduardo de Andrade; Vencovsky,Roland. |
| To assess the genetic diversity and genetic structure parameters, nine populations of Oryza glumaepatula from the Amazon biome, four from the Pantanal biome, and one collected at Rio Xingu, Mato Grosso, totaling 14 populations and 333 individuals were studied with isozyme markers. Six loci were evaluated showing a moderate allozyme variability (A = 1.21, P = 20.7%, Ho = 0.005, He = 0.060). The populations from the Pantanal biome showed higher diversity levels than the Amazon biome. High genetic differentiation among the populations, expected for self-fertilizing species, was observed (F ST=0.763), with lower differentiation found among the Pantanal populations (F ST=0.501). The average apparent outcrossing rate was higher for the Pantanal populations (t a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Isozymes; Oryza glumaepatula; Outcrossing rate; Populations. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500001 |
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| Ferreira,Jéssica Rosset; Pereira,Jorge Fernando; Turchetto,Caroline; Minella,Euclydes; Consoli,Luciano; Delatorre,Carla Andréa. |
| Abstract Barley is a major cereal grown widely and used in several food products, beverage production and animal fodder. Genetic diversity is a key component in breeding programs. We have analyzed the genetic diversity of barley accessions using microsatellite markers. The accessions were composed of wild and domesticated barley representing genotypes from six countries and three breeding programs in Brazil. A total of 280 alleles were detected, 36 unique to Brazilian barley. The marker Bmag120 showed the greatest polymorphism information content (PIC), with the highest mean value found on chromosome three, and the lowest on chromosomes four and six. The wild accessions presented the highest diversity followed by the foreign genotypes. Genetic analysis was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Domesticated barley; Genetic diversity; Hordeum vulgare ssp. vulgare; Hordeum vulgare ssp. spontaneum; Microsatellite markers. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100086 |
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| Registros recuperados: 291 | |
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