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Biochemical survey of the polar head of plant glycosylinositolphosphoceramides unravels broad diversity ArchiMer
Cacas, Jean-luc; Bure, Corinne; Furt, Fabienne; Maalouf, Jean-paul; Badoc, Alain; Cluzet, Stéphanie; Schmitter, Jean-marie; Antajan, Elvire; Mongrand, Sébastien.
Although Glycosyl-Inositol-Phospho-Ceramides (GIPCs) are the main sphingolipids of plant tissues, they remain poorly characterized in term of structures. This lack of information, notably with regard to polar heads, currently hampers the understanding of GIPC functions in biological systems. This situation prompted us to undertake a large scale-analysis of plant GIPCs: 23 plant species chosen in various phylogenetic groups were surveyed for their total GIPC content. GIPCs were extracted and their polar heads were characterized by negative ion MALDI and ESI mass spectrometry. Our data shed light on an unexpected broad diversity of GIPC distributions within Plantae, and the occurrence of yet-unreported GIPC structures in green and red algae. In monocots,...
Tipo: Text Palavras-chave: Glycosphingolipid; Glycosyl-Inositol-Phospho-Ceramide; GIPC; Mass spectrometry; MALDI-TOF; Polar head; Roots; Photosynthetic tissues; Berries.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00166/27689/25884.pdf
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Invariant Vα14 Natural Killer T Cell Activation by Edible Mushroom Acidic Glycosphingolipids OAK
NOZAKI, Hirofumi; ITONORI, Saki; SUGITA, Mutsumi; NAKAMURA, Kimihide; OHBA, Kiyoshi; OHNISHI, Masao; IMAI, Kunitoshi; IGARASHI, Yasuyuki; SUZUKI, Akemi; KUSHI, Yasunori; 中村, 公英; 大西, 正男; 今井, 邦俊.
https://www.jstage.jst.go.jp/article/bpb/33/4/33_4_580/_article
Palavras-chave: Alpha-galactosylceramide; Glycosphingolipid; Edible mushroom; Natural killer T cell; Mouse.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3599
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Role of Leishmania (Leishmania) amazonensis amastigote glycosphingolipids in macrophage infectivity BJMBR
Tanaka,A.K.; Gorin,P.A.J.; Takahashi,H.K.; Straus,A.H..
The role of glycosphingolipids (GSLs) present in amastigote forms of Leishmania (Leishmania) amazonensis during infection of macrophages was analyzed, with particular emphasis on GSLs presenting the terminal Galpß1-3Galpa disaccharide. Macrophage invasion by L. (L.) amazonensis amastigotes was reduced by 37% when the disaccharide Galpß1-3Galp (1 mM) was added to the culture medium. The putative macrophage receptor/lectin for ß-Gal-globotriaosylceramide (Galpß1-3Galpa1-4Galpß1-4Glc pß1-1Cer) and other structurally related GSLs from L. (L.) amazonensis amastigotes were analyzed by micelles and parasite binding assay to peritoneal macrophage proteins fractionated by SDS-PAGE under nonreducing conditions. Micelles containing purified amastigote GSLs or a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmania (Leishmania) amazonensis; Amastigote; Glycosphingolipid; Macrophage binding protein.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600008
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