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| Paula,Márcia Beatriz Cardoso de; Yokosawa,Jonny; Coutinho,Márcio Danilo Botrel; Silva,Paulo Lourenço; Ferraz,Rogério Avelar; Oliveira,Thelma Fátima Mattos; Queiróz,Divina Aparecida Oliveira. |
| In order to identify and characterize the agent of a suggestive clinical case of Gumboro disease (GD) that affected a 34-day-old broiler flock in Buriti Alegre (Goias State, Midwestern Brazil) in the year 2001, we carried out a combination of classic and modern virological methods. Histopathological analysis of the bursa revealed necrosis, presence of depleted follicles, some infiltration of heterophils, edema and formation of cystic cavities that are compatible with lesions observed in GD. Inoculation of embryonated eggs of specific pathogen-free (SPF) chickens with macerated bursa suspension resulted in embryo mortality and lesions which were also compatible with those caused by IBDV. A sample of bursa was submitted to a nested reverse... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken broiler flock; Gumboro disease; Infectious bursal disease virus; IBDV; RT-PCR; Molecular characterization. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300016 |
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