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Études écophysiologiques sur Dinophysis et sa chaîne alimentaire, et effets in vitro du dinoflagellé et de ses toxines sur les premiers stades de vie de deux modèles d'animaux marins (l’huître et le poisson) ArchiMer
Gaillard, Sylvain.
Harmful algal blooms of Dinophysis are responsible for diarrheic shellfish poisoning in human consumers of contaminated mollusks after bioaccumulation of their toxins. Dinophysis is a mixotrophic organism, which sequesters chloroplasts from a unique prey Mesodinium rubrum to perform its own photosynthesis, itself mixotrophic and feeds a cryptophyte, Teleaulax amphioxeia. The relationship between these three organisms is fundamental to understand the distribution and bloom dynamics of Dinophysis. In a first study, the effects of temperature, irradiance and pH on physiological aspects of T. amphioxeia were determined using a full factorial design before assessing the effect of contrasting physiological states after acclimation to two light intensities of T....
Tipo: Text Palavras-chave: Changement global; Crassostrea gigas; Cyprinodon variegatus; Dinophysis; Efflorescences; Mesodinium; Pecténotoxines; Teleaulax; Toxines diarrhéiques; Crassostrea gigas; Cyprinodon variegatus; Diarrheic toxins; Dinophysis; Global change; Harmful algal blooms; Mesodinium; Pectenotoxins; Teleaulax.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00666/77807/79981.pdf
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Eutrophication modelling chain for improved management strategies to prevent algal blooms in the Bay of Seine ArchiMer
Passy, Paul; Le Gendre, Romain; Garnier, Josette; Cugier, Philippe; Callens, Julie; Paris, Francois; Billen, Gilles; Riou, Philippe; Romero, Estela.
Eutrophication of the Seine estuary and the Bay of the Seine is a crucial environmental issue for the management of ecosystems and economic activities related to fisheries and tourism. A large quantity of nutrients, especially nitrogen, is brought to the coastal zone by the Seine River, the main input of that area, but also by smaller rivers along the Normandy coast. This large delivery of nitrogen leads to an imbalance between nitrogen (N), phosphorus (P) and silica (Si), which affects the growth of planktonic organisms and can exacerbate the occurrence of harmful algal blooms (HABs). These events can be damaging for shellfish fisheries, an important economic resource for the region. The study describes a new modelling chain coupling a riverine and a...
Tipo: Text Palavras-chave: Eutrophication; Harmful algal blooms; Nutrient load; Modelling; Water quality; Seine.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00300/41103/41542.pdf
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Evolutionary processes and cellular functions underlying divergence in Alexandrium minutum ArchiMer
Le Gac, Mickael; Metegnier, Gabriel; Chomerat, Nicolas; Malestroit, Pascale; Quere, Julien; Bouchez, Olivier; Siano, Raffaele; Destombe, Christophe; Guillou, Laure; Chapelle, Annie.
Understanding divergence in the highly dispersive and seemingly homogeneous pelagic environment for organisms living as free drifters in the water column remains a challenge. Here, we analyzed the transcriptome wide mRNA sequences, as well as the morphology of 18 strains of Alexandrium minutum, a dinoflagellate responsible for Harmful Algal Blooms worldwide, to investigate the functional bases of a divergence event. Analysis of the joint site frequency spectrum (JSFS) pointed toward an ancestral divergence in complete isolations followed by a secondary contact resulting in gene flow between the two diverging groups, but heterogeneous across sites. The sites displaying fixed SNPs were associated with a highly restricted gene flow and a strong...
Tipo: Text Palavras-chave: Dinoflagellates; Harmful algal blooms; Populations Genomics; Pseudocryptic species; Speciation.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00347/45857/45529.pdf
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Exposure to the toxic dinoflagellate Alexandrium catenella modulates juvenile oyster Crassostrea gigas hemocyte variables subjected to different biotic conditions ArchiMer
Lassudrie, Malwenn; Soudant, Philippe; Nicolas, Jean-louis; Miner, Philippe; Le Grand, Jacqueline; Lambert, Christophe; Le Goic, Nelly; Hegaret, Helene; Fabioux, Caroline.
The Pacific oyster Crassostrea gigas is an important commercial species cultured throughout the world. Oyster production practices often include transfers of animals into new environments that can be stressful, especially at young ages. This study was undertaken to determine if a toxic Alexandrium bloom, occurring repeatedly in French oyster beds, could modulate juvenile oyster cellular immune responses (i.e. hemocyte variables). We simulated planting on commercial beds by conducting a cohabitation exposure of juvenile, “specific pathogen-free” (SPF) oysters (naïve from the environment) with previously field-exposed oysters to induce interactions with new microorganisms. Indeed, toxic Alexandrium spp. exposures have been reported to modulate bivalve...
Tipo: Text Palavras-chave: Crassostrea gigas; Alexandrium; Harmful algal blooms; Hemocyte; Microbial challenge.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00314/42523/41894.pdf
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First report of the potentially toxic marine diatom Pseudo-nitzschia simulans (Bacillariophyceae) from the East Australian Current ArchiMer
Ajani, Penelope A.; Lim, Hong Chang; Verma, Arjun; Lassudrie, Malwenn; Mcbean, Katie; Doblin, Martina A.; Murray, Shauna A..
Certain species of the marine diatom genus Pseudo‐nitzschia are responsible for the production of the domoic acid (DA), a neurotoxin that can bioaccumulate in the food chain and cause amnesic shellfish poisoning (ASP) in animals and humans. This study extends our knowledge by reporting on the first observation of the potentially toxic species Pseudo‐nitzschia simulans from this region. One clonal strain of P. simulans was isolated from the East Australian Current and characterized using light and transmission electron microscopy, and phylogenetic analyses based on regions of the internal transcribed spacer (ITS) and the D1–D3 region of the large subunit (LSU) of the nuclear‐encoded ribosomal deoxyribonucleic acid (rDNA), as well as examined for DA...
Tipo: Text Palavras-chave: Domoic acid; East Australian current; Harmful algal blooms; Phytoplankton; Shellfish.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00618/73047/75313.pdf
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Harmful phytoplankton blooms and fish mortality in a eutrophicated reservoir of northeast Brazil BABT
Chellappa,Naithirithi Tiruvenkatachary; Chellappa,Sarah Laxhmi; Chellappa,Sathyabama.
The aim of this work was to study the eutrophication in the tropical freshwater ecosystems and the consequent cyanobacterial bloom formation and economical damage to fisheries and harmful effects to public health. Mass fish mortality due to toxin producing cyanobacterial blooms was registered during December 2003 in Marechal Dutra Reservoir, Acari/RN, Northeast Brazil. Phytoplankton and fish samplings were carried out on alternate days during the episode of fish mortality and monthly during January to June 2004. The cyanobacterial toxin was identified and quantified from the seston samples and liver of the dead fishes using the standard HPLC method. The results indicated that the toxic blooms of Cylindrospermopsis raciborskii and Microcystis aeruginosa...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Harmful algal blooms; Anthropogenic activity; Fish mortality; Reservoir; Public health.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400022
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Interaction between toxic dinoflagellate Alexandrium catenella exposure and disease associated with herpesvirus OsHV-1μVar in Pacific oyster spat Crassostrea gigas ArchiMer
Lassudrie, Malwenn; Soudant, Philippe; Nicolas, Jean-louis; Fabioux, Caroline; Lambert, Christophe; Miner, Philippe; Le Grand, Jacqueline; Petton, Bruno; Hegaret, Helene.
Blooms of toxic dinoflagellates can co-occur with mass mortality events associated with herpesvirus OsHV-1 μVar infection that have been decimating Pacific oyster Crassostrea gigas spat and juveniles every summer since 2008 in France. This study investigated the possible effect of a harmful dinoflagellate, Alexandrium catenella, a producer of Paralytic Shellfish Toxins (PSTs), upon the oyster spat–herpesvirus interaction. Oyster spat from a hatchery were challenged by cohabitation with oysters contaminated in the field with OsHV-1 μVar and possibly other pathogens. Simultaneously, the oysters were exposed to cultured A. catenella. Infection with OsHV-1 μVar and PST accumulation were measured after 4 days of experimental exposure. Exposure to Alexandrium...
Tipo: Text Palavras-chave: Crassostrea gigas; OsHV-1 mu Var; Harmful algal blooms; Alexandrium; Host-pathogen interaction; PST accumulation.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00269/38000/36101.pdf
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Oceans and Human Health: A Rising Tide of Challenges and Opportunities for Europe ArchiMer
Fleming, L.e.; Mcdonough, N.; Austen, M.; Mee, L.; Moore, Michael; Hess, Philipp; Depledge, M.h.; White, M.; Philippart, Katja; Bradbrook, P.; Smalley, A..
The European Marine Board recently published a position paper on linking oceans and human health as a strategic research priority for Europe. With this position paper as a reference, the March 2014 Cornwall Oceans and Human Health Workshop brought together key scientists, policy makers, funders, business, and non governmental organisations from Europe and the US to review the recent interdisciplinary and cutting edge research in oceans and human health specifically the growing evidence of the impacts of oceans and seas on human health and wellbeing (and the effects of humans on the oceans). These impacts are a complex mixture of negative influences (e.g. from climate change and extreme weather to harmful algal blooms and chemical pollution) and beneficial...
Tipo: Text Palavras-chave: Harmful algal blooms; Microbial pollution; Anthropogenic chemicals; Marine biotechnology; Climate change; Ocean acidification; Fisheries; Aquaculture; Seafood; Blue carbon; Marine energy; Blue gym; Ocean literacy; Risks; Benefits; EU Policy.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00192/30328/29066.pdf
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Ostreopsis cf. ovata (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil ArchiMer
Nascimento, Silvia M; Neves, Raquel Af; De'Carli, Gabriela Al; Borsato, Geovanna T; Da Silva, Rodrigo Af; Melo, Guilherme A; De Morais, Agatha M; Cockell, Thais C; Fraga, Santiago; Menezes-salgueiro, Adriana D; Mafra, Luis L Jr; Hess, Philipp; Salgueiro, Fabiano.
Recurrent blooms of Ostreopsis cf. ovata have been reported in Brazil and the Mediterranean Sea with associated ecological, and in the latter case, health impacts. Molecular data based on the D1–D3 and D8–D10 regions of the LSU rDNA and ITS loci, and the morphology of O. cf. ovata isolates and field populations from locations along the Brazilian tropical and subtropical coastal regions and three oceanic islands are presented. Additional ITS sequences from three single cells from the tropical coast are provided. Toxin profiles and quantities of PLTX and their analogues; OVTXs; contained in cells from two clonal cultures and two field blooms from Rio de Janeiro were investigated. Morphology was examined using both light and epifluorescence microscopy....
Tipo: Text Palavras-chave: Benthic dinoflagellates; Harmful algal blooms; Ovatoxins; Taxonomy; Morphometry.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00604/71594/70026.pdf
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Phytoplankton assemblages and characterization of a Dinophysis acuminata population during an upwelling-downwelling cycle ArchiMer
Gonzalez-gil, Sonsoles; Velo-suarez, Lourdes; Gentien, Patrick; Ramilo, Isabel; Reguera, Beatriz.
The distributions of Dinophysis acuminata, its potential prey Myrionecta rubra, and the microplankton populations associated with them, were studied in Ria de Pontevedra (NW Spain) during a 2 wk cruise that covered an upwelling-downwelling cycle, and during intensive sampling over 21 h at the end of the cruise. Special attention was focused on the characterization and physiological condition of D. acuminata. There was vertical segregation between a phytoplankton assemblage that was dominated by upwelling-promoted diatoms and another assemblage that was dominated by small dinoflagellates, located in the warmer surface layer (0 to 5 m) where D. acuminata was observed. High spatio-temporal variability was observed in the frequency of cells containing starch...
Tipo: Text Palavras-chave: Harmful algal blooms; Plankton communities; Dinophysis acuminata; Myrionecta rubra; Predator-prey interactions.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11296/7822.pdf
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POTENTIALLY HARMFUL CYANOBACTERIA IN OYSTER BANKS OF TÉRMINOS LAGOON, SOUTHEASTERN GULF OF MEXICO Acta biol.Colomb.
POOT-DELGADO,Carlos Antonio; OKOLODKOV,Yuri B.; AKÉ-CASTILLO,José Antolin; RENDÓN von OSTEN.,Jaime.
ABSTRACT Cyanobacteria inhabit hypersaline, marine and freshwater environments. Some toxic and non-toxic species can form harmful blooms. The aim of this study was to identify potentially harmful cyanobacterial species in the oyster banks of Términos Lagoon, the southeastern Gulf of Mexico. Six sample sites (up to 2-m depth) were monitored monthly from August 2012 to September 2013. Water temperature, salinity, pH, dissolved oxygen saturation (% DO), inorganic nutrients and abundance of cyanobacteria were determined. Temperature and salinity were characterized by marked seasonal differences (26.8 to 30.6 °C and 6.1 to 19.5, respectively). The pH values (ranging from 7.1 to 8.4) and the % DO (88.4 to 118.2 %) suggest a predominance of photosynthetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Annual cycle; Cyanobacteria; Eutrophication; Gulf of Mexico; Harmful algal blooms.
Ano: 2018 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2018000100051
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Resting Cyst Distribution and Molecular Identification of the Harmful Dinoflagellate Margalefidinium polykrikoides (Gymnodiniales, Dinophyceae) in Lampung Bay, Sumatra, Indonesia ArchiMer
Thoha, Hikmah; Muawanah,; Bayu Intan, Mariana D.; Rachman, Arief; Sianturi, Oksto Ridho; Sidabutar, Tumpak; Iwataki, Mitsunori; Takahashi, Kazuya; Avarre, Jean-christophe; Masseret, Estelle.
Margalefidinium polykrikoides, an unarmored dinoflagellate, was suspected to be the causative agent of the harmful algal blooms – associated with massive fish mortalities – that have occurred continually in Lampung Bay, Indonesia, since the first bloom event in October 2012. In this study, after examination of the morphology of putative M. polykrikoides-like cysts sampled in bottom sediments, cyst bed distribution of this harmful species was explored in the inner bay. Sediment samples showed that resting cysts, including several morphotypes previously reported as M. polykrikoides, were most abundant on the northern coast of Lampung Bay, ranging from 20.6 to 645.6 cysts g-1 dry sediment. Molecular phylogeny inferred from LSU rDNA revealed that the so-called...
Tipo: Text Palavras-chave: Margalefidinium polykrikoides; Dinoflagellate; Harmful algal blooms; Resting and hyaline cysts; Indonesia.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00489/60086/63423.pdf
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Solid Phase Adsorption Toxin Tracking (SPATT) Technology for the Monitoring of Aquatic Toxins: A Review ArchiMer
Roue, Melanie; Darius, Helene Taiana; Chinain, Mireille.
The Solid Phase Adsorption Toxin Tracking (SPATT) technology, first introduced in 2004, uses porous synthetic resins capable of passively adsorbing toxins produced by harmful microalgae or cyanobacteria and dissolved in the water. This method allows for the detection of toxic compounds directly in the water column and offers numerous advantages over current monitoring techniques (e.g., shellfish or fish testing and microalgae/cyanobacteria cell detection), despite some limitations. Numerous laboratory and field studies, testing different adsorbent substrates of which Diaion((R)) HP20 resin appears to be the most versatile substrate, have been carried out worldwide to assess the applicability of these passive monitoring devices to the detection of toxins...
Tipo: Text Palavras-chave: SPATT technology; Passive monitoring; Risk assessment; Harmful algal blooms; Aquatic toxins; Microalgae; Cyanobacteria.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00660/77225/78667.pdf
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Technological adaptation to harmful algal blooms : socioeconomic consequences for the shellfish farming sector in Bourgneuf Bay (France) ArchiMer
Perez Agundez, José A.; Raux, Pascal; Girard, Sophie; Mongruel, Remi.
The economic impacts of harmful algal blooms (HABs) on the shellfish farming sector depend on their frequency, duration and intensity. Safeguarding storage and accelerated detoxification are technical solutions that could mitigate the effects of these events. This article first analyzes the economic feasibility of the technological changes that can be adopted by the shellfish farming sector in France. It then examines their associated social impacts. Finally, an application is carried out on the Bourgneuf Bay production area. The economic analysis addresses three issues related to HABs: (1) the economic performance of the shellfish farms in Bourgneuf Bay, (2) the costs of these new technologies, and (3) the economic viability of such an investment. Results...
Tipo: Text Palavras-chave: Detoxification; Economic viability; Harmful algal blooms; Safeguarding storage; Shellfish farming.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00162/27279/30695.pdf
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Testing a Microarray to Detect and Monitor Toxic Microalgae in Arcachon Bay in France ArchiMer
Kegel, Jessica; Del Amo, Yolanda; Costes, Laurence; Medlin, Linda.
Harmful algal blooms (HABs) occur worldwide, causing health problems and economic damages to fisheries and tourism. Monitoring agencies are therefore essential, yet monitoring is based only on time-consuming light microscopy, a level at which a correct identification can be limited by insufficient morphological characters. The project MIDTAL (Microarray Detection of Toxic Algae)—an FP7-funded EU project—used rRNA genes (SSU and LSU) as a target on microarrays to identify toxic species. Furthermore, toxins were detected with a newly developed multiplex optical Surface Plasmon Resonance biosensor (Multi SPR) and compared with an enzyme-linked immunosorbent assay (ELISA). In this study, we demonstrate the latest generation of MIDTAL microarrays (version 3)...
Tipo: Text Palavras-chave: Oligonucleotide microarrays; Molecular monitoring; Harmful algal blooms; HABs; Toxic microalgae; 18S/28S ribosomal RNA; LSU/SSU; RNA hybridization; Environmental water samples.
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00376/48678/49011.pdf
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The 2016 red tide crisis in southern Chile: Possible influence of the mass oceanic dumping of dead salmons ArchiMer
Armijo, Julien; Oerder, Vera; Auger, Pierre-amaël; Bravo, Angela; Molina, Ernesto.
In 2016, a massive harmful algal bloom (HAB) of Alexandrium catenella around Chiloé island caused one of the major socio-ecological crisis in Chilean history. This red tide occurred in two distinct pulses, the second, most anomalous, bursting with extreme toxicity on the Pacific coast, weeks after the highly controversial dumping off Chiloé of 4,700 t of rotting salmons, killed by a previous HAB of Pseudochattonella verruculosa. We study the transport of this pollution, analyzing the physical oceanographic conditions during and after the dumping. We find that a cyclonic gyre was present between the dumping site and the coast, visible in satellite altimetry and sea surface temperature data. Using Lagrangian simulations, we confirm that near-surface currents...
Tipo: Text Palavras-chave: Harmful algal blooms; Aquaculture; Pollution control; Ocean transport; Ecological crisis; Risk management.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00593/70486/69183.pdf
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Trajectories of Changes in Phytoplankton Biomass, Phaeocystis globosa and Diatom (incl. Pseudo-nitzschia sp.) Abundances Related to Nutrient Pressures in the Eastern English Channel, Southern North Sea ArchiMer
Lefebvre, Alain; Dezécache, Camille.
The phytoplankton compartment is particularly reactive to changes in nutrient concentration and is used as a quality indicator. Using a simple numerical approach, the response of emblematic harmful taxa from the eastern English Channel and southern North Sea to changes in nutrient inputs was studied. The method is based on a diachronic approach using averaged maxima over sliding periods of six years (1994–2018). This gave a final dataset containing pairs of points (number of years) for explained and explanatory variables. The temporal trajectory of the relationship between each pair of variables was then highlighted. Changes were represented as long-term trajectories that allowed a comparison to a reference/average situation. In addition, the relevance of...
Tipo: Text Palavras-chave: Harmful algal blooms; Eutrophication; Trajectory; Phaeocystis globosa; Pseudo-nitzchiacomplex; Oslo and Paris Convention OSPAR; Water Framework Directive (WFD); Marine Strategy Framework Directive (MSFD).
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00632/74400/74103.pdf
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Willingness-to-Pay for Red Tide Prevention, Mitigation, and Control Strategies: A Case Study of Florida Coastal Residents AgEcon
Lucas, Kristen M.; Larkin, Sherry L.; Adams, Charles M..
Harmful algal blooms (HABS) are natural events with ecological and economic consequences worldwide. Different nations and regions have adapted to HABS in a variety of ways including distinct strategies designed to prevent, control and or mitigate the negative effects of HAB events. In Florida, Karenia brevis is the algae species that has accounted for nearly all of blooms. This algae species is unique in that the toxins produced during the bloom are a neurotoxin that can kill fish and marine mammals and become airborne and affect the respiratory system of humans. The fact that such blooms, referred to locally as “red tides” since a bloom can discolor the water a reddish-brown color, can affect humans is potentially disastrous to a state like Florida that...
Tipo: Conference Paper or Presentation Palavras-chave: Harmful algal blooms; Florida; Dichotomous choice; Willingness to pay; Environmental Economics and Policy.
Ano: 2010 URL: http://purl.umn.edu/56498
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