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Registros recuperados: 31
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A sensitive bioassay for destruxins, cyclodepsipeptides from the culture filtrates of the entomopathogenic fungus Metarhizium anisopliae (Metsch.) Sorok. Anais da SEB
Samuels,Richard I..
Destruxin A, a toxic cyclodepsipeptide, was purified from culture filtrates of the entomopathogenic fungus Metarhizium anisopliae (Metsch.) Sorok. by Reverse-Phase HPLC, and assayed for its effects on a larval Manduca sexta (L.) (Lepidoptera: Sphingidae) heart preparation. Destruxin A was found to cause reversible and dose-dependent acceleration of the heart beat. The heart bioassay was highly sensitive to pulse applications of destruxin A at concentrations as low as 35 pmol. Because of its high level of sensitivity, the M. sexta heart bioassay could be used to detect and quantify destruxins in tissue extracts from mycosed insects or to confirm bioactivity of HPLC fractions. The site of action of Destruxin A was not determined. However, this toxin did not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Manduca sexta; Heart; Toxin; Peptide; Destruxin.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000200009
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Aortic body tumors in dogs from Northern Paraná, Brazil Ciência Rural
Headley,Selwyn Arlington; Bracarense,Ana Paula; Nakagawa,Tizianne; Moreno,Kleber; Pereira,Patrícia Mendes; Reis,Antônio Carlos Faria dos.
This article describes the occurrence of aortic body tumors in four dogs from Southern Brazil. All dogs were males, more than 9 year of age; most of these were from the Boxer breed of dogs. The animals had large neoplastic growths at the base of the heart, which demonstrated histologic characteristics consistent with that of aortic body tumor. By immunohistochemistry, tissue sections from all dogs reacted positively to neuron specific enolase. These cases might represent the first description of this tumor in dogs from Brazil, as confirmed by immunohistochemistry.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Heart; Aortic body tumor; Immunohistochemistry; Neuron specific enolase.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000600045
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Apoptosis and Histopathology of the Heart after Renal Ischemia-Reperfusion in Male Rat Running title: Ischemia-Reperfusion Injury BABT
Alihemmati,Alireza; Yousefi,Hadi; Ahmadiasl,Naser; Habibi,Parisa.
ABSTRACT Ischemia-reperfusion injury was seen in strokes, myocardial infarctions, acute kidney injury, mesenteric ischemia, liver and systemic shock. Renal ischemia-reperfusion is more importance in the setting of kidney transplantation that affects distant organs. In this study forty Male Albino Wistar rats (200-250g) were randomly divided in four group (n=10) including control, sham operation group, nephrectomy and IRI group. All rats anesthetized with intraperitoneal injection of ketamine (50 mg/kg) and xylazine (10 mg/kg) and maintained the core body temperature at approximately 37°C. For inducing IRI group, it was performed right nephrectomy, and in continuing, the left kidney pedicle occluded to 45 min via nontraumatic microvascular clamp for making...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Renal Ischemia-reperfusion injury; Glycogen accumulation; Apoptosis; Heart; Rat.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100443
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CaMKIIdelta overexpression in hypertrophy and heart failure: cellular consequences for excitation-contraction coupling BJMBR
Maier,L.S..
Ca/calmodulin-dependent protein kinase IIdelta (CaMKIIdelta) is the predominant isoform in the heart. During excitation-contraction coupling (ECC) CaMKII phosphorylates several Ca-handling proteins including ryanodine receptors (RyR), phospholamban, and L-type Ca channels. CaMKII expression and activity have been shown to correlate positively with impaired ejection fraction in the myocardium of patients with heart failure and CaMKII has been proposed to be a possible compensatory mechanism to keep hearts from complete failure. However, in addition to these acute effects on ECC, CaMKII was shown to be involved in hypertrophic signaling, termed excitation-transcription coupling (ETC). Thus, animal models have shown that overexpression of nuclear isoform...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calcium; Calmodulin; CaM kinase; Excitation-contraction coupling; Heart.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900002
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Cardiac and pulmonary alterations in symptomatic and asymptomatic dogs infectednaturally with Leishmania (Leishmania) chagasi BJMBR
Alves,G.B.B.; Pinho,F.A.; Silva,S.M.M.S.; Cruz,M.S.P.; Costa,F.A.L..
Fifteen symptomatic and seven asymptomatic dogs infected naturally with Leishmania chagasi were examined in order to identify the presence of parasites and changes in heart and lung. Histopathological, cytological, and immunohistochemical analyses were performed on samples of heart and lung tissues. An inflammatory reaction characterized by inflammatory mononuclear, perivascular and intermuscular infiltrates was observed in both symptomatic and asymptomatic animals on histopathological analysis of the heart. In the lung, there was thickening of the alveolar septa due to congestion, edema, inflammatory infiltrate, and fibroblast proliferation. A focal reaction was observed although a diffuse reaction was present in both groups. On cytological examination,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Dog; Lungs; Heart.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300014
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Caspase-3 activation and increased procollagen type I in irradiated hearts Anais da ABC (AABC)
Ferreira-Machado,Samara C.; Salata,Camila; Rocha,Nazareth N.; Corrêa,Alexandre F.S.; Côrte-Real,Suzana; Peregrino,Antônio A.F.; Campos,Vera M.A. de; Andrade,Cherley B.V.; Bernardo-Filho,Mario; Cabral-Neto,Januário B.; Dealmeida,Carlos E..
The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-ß1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-ß1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoptosis; Caspase-3; Fibrosis; Heart; Ionizing radiation; TGF-β1.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100215
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Characterization of coronary arteries in Giant Anteater (Myrmecophaga tridactyla: Myrmecophagidae) Arq. Bras. Med. Vet. Zootec.
Cruvinel,C.A.T.; Cruvinel,T.M.A.; Aires,L.P.N.; Rodrigues,R.F.; Melo,A.P.F..
ABSTRACT Were used twelve (12) adult anteaters (Myrmecophaga tridactyla), adults, 6 (six) males and 6 (six) females, weighing from 20 to 27.32kg from free life. The thoracic cavity was opened until visualization of the whole heart and lungs and later injection of the coronary vessels. The right coronary artery emerged through a single coronary ostium of the aorta, 50%, emitting the intermediate branch and the subsinuous interventricular branch, had a path directed to the subsurface interventricular groove. In the other 50%, the right coronary artery was not present, showing only its branches, intermediate branch and subsurface interventricular branch with emergence of the aorta. Left coronary artery presented, in 83.33%, origin from the aorta in single...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heart; Coronary artery; Wild animals; Giant anteaters.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200545
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Characterization of rat heart alkaline phosphatase isoenzymes and modulation of activity BJMBR
Mota,A.; Silva,P.; Neves,D.; Lemos,C.; Calhau,C.; Torres,D.; Martel,F.; Fraga,H.; Ribeiro,L.; Alçada,M.N.M.P.; Pinho,M.J.; Negrão,M.R.; Pedrosa,R.; Guerreiro,S.; Guimarães,J.T.; Azevedo,I.; Martins,M.J..
Alkaline phosphatase (ALP) is important in calcification and its expression seems to be associated with the inflammatory process. We investigated the in vitro acute effects of compounds used for the prevention or treatment of cardiovascular diseases on total ALP activity from male Wistar rat heart homogenate. ALP activity was determined by quantifying, at 410 nm, the p-nitrophenol released from p-nitrophenylphosphate (substrate in Tris buffer, pH 10.4). Using specific inhibitors of ALP activity and the reverse transcription-polymerase chain reaction, we showed that the rat heart had high ALP activity (31.73 ± 3.43 nmol p-nitrophenol·mg protein-1·min-1): mainly tissue-nonspecific ALP but also tissue-specific intestinal ALP type II. Both ALP isoenzymes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heart; Alkaline phosphatase; Polyphenol-rich beverages; Steroid hormones; Methylxanthines.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700009
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Chronic hypertension alters the expression of Cx43 in cardiovascular muscle cells BJMBR
Haefliger,J.-A.; Meda,P..
Connexin43 (Cx43), the predominant gap junction protein of muscle cells in vessels and heart, is involved in the control of cell-to-cell communication and is thought to modulate the contractility of the vascular wall and the electrical coupling of cardiac myocytes. We have investigated the effects of arterial hypertension on the expression of Cx43 in aorta and heart in three different models of experimental hypertension. Rats were made hypertensive either by clipping one renal artery (two kidney, one-clip renal (2K,1C) model) by administration of deoxycorticosterone and salt (DOCA-salt model) or by inhibiting nitric oxide synthase with NG-nitro-L-arginine methyl ester (L-NAME model). After 4 weeks, rats of the three models showed a similar increase in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell-cell communication; Gap junctions; Connexins; Vasculature; Heart; Hypertension; Blood pressure; Renin; Deoxycorticosterone; Nitric oxide; Distensibility.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400009
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Chronic valvular disease: correlation between clinical, electrocardiographic, radiographic and echocardiographic aspects in dogs Arq. Bras. Med. Vet. Zootec.
Soares,E.C.; Larsson,M.H.M.A.; Yamato,R.J..
Echocardiographic aspects of chronic mitral valvular disease were studied and compared to physical, radiographic and electrocardiographic aspects. Seventy dogs were used, and clinical examination, thoracic radiography, electrocardiogram and echocardiogram were performed. Correlations between regurgitation severity with cardiac failure functional class and murmur intensity were observed. The electrocardiogram showed a low sensibility in detecting cardiac chamber enlargement, caused by mitral regurgitation. All the dogs with severe mitral regurgitation showed cardiomegaly according to thoracic radiographies.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Heart; Cardiac failure; Echocardiography; Cardiac valve.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000400003
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Echocardiographic reference ranges for sedated healthy peccaries ( Tayassu tajacu, Linnaeus , 1758 ) Arq. Bras. Med. Vet. Zootec.
Moura,L.S.; Rodrigues,R.P.S.; Silva,A.B.S.; Pessoa,G.T.; Sousa,F.C.A.; Barbosa,M.A.P.S.; Souza,A.B.; Alves,J.J.R.P.; Macedo,K.V.; Diniz,A.N.; Silva,E.G.; Araújo,J.R.; Marques,D.C.; Cavalcante-Filho,M.F.; Alves,F.R..
ABSTRACT Peccaries are wild mammals belonging to the Tayassu genus that are found almost everywhere in the Americas and have demonstrated great potential as an experimental model for scientific investigations. Twelve healthy adult animals were sedated to perform echocardiographic examinations in B, M and Doppler mode. The variables that exhibited statistically significant correlation coefficients with weight were LVFWd, LVIDd, LVIDs, E wave, A’RV, MAM, and TAPSE. The HR exhibited a negative relationship with the IVRT. The LA variable showed a positive correlation with the AO. The MAM exhibited correlations with the LVIDd and LVIDs. The TAPSE showed positive correlations with the E’RV and A’RV. The present study provides the first reference values for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cardiology; Wild animals; Diagnostic imaging; Heart; Hemodynamics.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100068
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Effect of acute nitric oxide synthase inhibition in the modulation of heart rate in rats BJMBR
Fellet,A.L.; Di Verniero,C.; Arza,P.; Tomat,A.; Varela,A.; Arranz,C.; Balaszczuk,A.M..
Acute nitric oxide synthase inhibition with N G-nitro-L-arginine methyl ester (L-NAME) on chronotropic and pressor responses was studied in anesthetized intact rats and rats submitted to partial and complete autonomic blockade. Blood pressure and heart rate were monitored intra-arterially. Intravenous L-NAME injection (7.5 mg/kg) elicited the same hypertensive response in intact rats and in rats with partial (ganglionic and parasympathetic blockade) and complete autonomic blockade (38 ± 3, 55 ± 6, 54 ± 5, 45 ± 5 mmHg, respectively; N = 9, P = NS). L-NAME-induced bradycardia at the time when blood pressure reached the peak plateau was similar in intact rats and in rats with partial autonomic blockade (43 ± 8, 38 ± 5, 46 ± 6 bpm, respectively; N = 9, P =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitric oxide; Nitric oxide synthase; Autonomic nervous system; Heart rate; Heart.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500015
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Effects of gene therapy on cardiovascular symptoms of lysosomal storage diseases Genet. Mol. Biol.
Poletto,Edina; Pasqualim,Gabriela; Giugliani,Roberto; Matte,Ursula; Baldo,Guilherme.
Abstract Lysosomal storage diseases (LSDs) are inherited conditions caused by impaired lysosomal function and consequent substrate storage, leading to a range of clinical manifestations, including cardiovascular disease. This may lead to significant symptoms and even cardiac failure, which is an important cause of death among patients. Currently available treatments do not completely correct cardiac involvement in the LSDs. Gene therapy has been tested as a therapeutic alternative with promising results for the heart disease. In this review, we present the results of different approaches of gene therapy for LSDs, mainly in animal models, and its effects in the heart, focusing on protocols with cardiac functional analysis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lysosomal storage disease; Gene therapy; Cardiovascular disease; Animal models; Heart.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200261
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Epilepsy-induced electrocardiographic alterations following cardiac ischemia and reperfusion in rats BJMBR
Tavares,J.G.P.; Vasques,E.R.; Arida,R.M.; Cavalheiro,E.A.; Cabral,F.R.; Torres,L.B.; Menezes-Rodrigues,F.S.; Jurkiewicz,A.; Caricati-Neto,A.; Godoy,C.M.G.; Gomes da Silva,S..
The present study evaluated electrocardiographic alterations in rats with epilepsy submitted to an acute myocardial infarction (AMI) model induced by cardiac ischemia and reperfusion. Rats were randomly divided into two groups: control (n=12) and epilepsy (n=14). It was found that rats with epilepsy presented a significant reduction in atrioventricular block incidence following the ischemia and reperfusion procedure. In addition, significant alterations were observed in electrocardiogram intervals during the stabilization, ischemia, and reperfusion periods of rats with epilepsy compared to control rats. It was noted that rats with epilepsy presented a significant increase in the QRS interval during the stabilization period in relation to control rats...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epilepsy; Heart; Acute myocardial infarction; Ischemia; Reperfusion.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200140
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Future ocean warming may prove beneficial for the northern population of European seabass, but ocean acidification will not ArchiMer
Howald, Sarah; Cominassi, Louise; Le Bayon, Nicolas; Claireaux, Guy; Mark, Felix C..
The world's oceans are acidifying and warming as a result of increasing atmospheric CO2 concentrations. The thermal tolerance of fish greatly depends on the cardiovascular ability to supply the tissues with oxygen. The highly oxygen-dependent heart mitochondria thus might play a key role in shaping an organism's tolerance to temperature. The present study aimed to investigate the effects of acute and chronic warming on the respiratory capacity of European sea bass (Dicentrarchus labrax L.) heart mitochondria. We hypothesized that acute warming would impair mitochondrial respiratory capacity, but be compensated for by life-time conditioning. Increasing PCO2 may additionally cause shifts in metabolic pathways by inhibiting several enzymes of the cellular...
Tipo: Text Palavras-chave: Mitochondrial respiration; Developmental acclimation; Temperate teleost; Heart.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00592/70446/68556.pdf
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Imidazoline receptors in the heart: a novel target and a novel mechanism of action that involves atrial natriuretic peptides BJMBR
Mukaddam-Daher,S.; Gutkowska,J..
Chronic stimulation of sympathetic nervous activity contributes to the development and maintenance of hypertension, leading to left ventricular hypertrophy (LVH), arrhythmias and cardiac death. Moxonidine, an imidazoline antihypertensive compound that preferentially activates imidazoline receptors in brainstem rostroventrolateral medulla, suppresses sympathetic activation and reverses LVH. We have identified imidazoline receptors in the heart atria and ventricles, and shown that atrial I1-receptors are up-regulated in spontaneously hypertensive rats (SHR), and ventricular I1-receptors are up-regulated in hamster and human heart failure. Furthermore, cardiac I1-receptor binding decreased after chronic in vivo exposure to moxonidine. These studies implied...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sympathetic nerve activity; Imidazoline receptors; Moxonidine; Atrial natriuretic peptide; Brain natriuretic peptide; Hypertension; Heart.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800015
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Impact of the Training Program on Lipid Profile and Cardiac Health Biol. Res.
BURNEIKO,REGINA COELI; DINIZ,YEDA SANT'ANA; FAINE,LUCIANE APARECIDA; MACHADO GALHARDI,CRISTIANO; PADOVANI,CARLOS ROBERTO; NOVELLI,ETHEL LOURENZI B.; CICOGNA,ANTONIO CARLOS.
The aim of this study was to investigate the effects of training programs on serum lipid profile and myocardial oxidative stress. Male Wistar rats (2 mo-old) were divided into three groups (n=8): sedentary (S), loadless trained (T) and trained-overload 2% body weight (TL). T and TL were trained through swimming for 9 weeks. T and TL rats had increased myocardial lipoperoxide (TBA) and lipid hydroperoxide (HP), whereas HP was higher in TL than in T animals. Superoxide dismutase (SOD) activities were lowest in TL. Myocardial glutathione peroxidase (GSH-Px) was lower in TL than in T and S rats. TL decreased HDL-cholesterol and increased LDL-cholesterol. The serum lactate dehydrogenase and TBA were increased, while SOD and GSH-Px activities were decreased in...
Tipo: Journal article Palavras-chave: Training; Lipid profile; Oxidative stress; Heart; Serum.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100006
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Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium BJMBR
Oliveira,E.D.; Leite,T.S.; Silva,B.A.; Conde-Garcia,E.A..
The objective of the present investigation was to determine the contractile effect of crude and acetone leaf extracts of Citrus sinensis (L.) Osb. on mammalian myocardium. Crude leaf extracts have been used in folk medicine to treat neurological disorders. Some flavonoids isolated from this plant presented a positive inotropic effect on myocardium. This motivated us to test the extracts on the atria of guinea pigs of both sexes (300-500 g) and surprisingly we observed inotropic depression instead of an increase in force. The maximum effect of the crude extract was 79.4 ± 8.1% of the control force amplitude (N = 5 hearts, 10 trials, 27 ± 0.1ºC, stimulus: 2 Hz, 400 V, 0.5 ms). The EC50 for crude, ethanol, acetic, aqueous, and acetone extracts was 300, 300,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Contractility; Heart; Myocardium; Woodworth phenomenon.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100017
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Intraspecific individual variation of temperature tolerance associated with oxygen demand in the European sea bass (Dicentrarchus labrax) ArchiMer
Ozolina, Karlina; Shiels, Holly A.; Ollivier, Helene; Claireaux, Guy.
The European sea bass (Dicentrarchus labrax) is an economically important fish native to the Mediterranean and Northern Atlantic. Its complex life cycle involves many migrations through temperature gradients that affect the energetic demands of swimming. Previous studies have shown large intraspecific variation in swimming performance and temperature tolerance, which could include deleterious and advantageous traits under the evolutionary pressure of climate change. However, little is known of the underlying determinants of this individual variation. We investigated individual variation in temperature tolerance in 30 sea bass by exposing them to a warm temperature challenge test. The eight most temperature-tolerant and eight most temperature-sensitive fish...
Tipo: Text Palavras-chave: Aerobic scope; Climate change; Fish; Heart; Individual variability; Swimming.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00337/44827/44369.pdf
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Lead reduces tension development and the myosin ATPase activity of the rat right ventricular myocardium BJMBR
Vassallo,D.V.; Lebarch,E.C.; Moreira,C.M.; Wiggers,G.A.; Stefanon,I..
Lead (Pb2+) poisoning causes hypertension, but little is known regarding its acute effects on cardiac contractility. To evaluate these effects, force was measured in right ventricular strips that were contracting isometrically in 45 male Wistar rats (250-300 g) before and after the addition of increasing concentrations of lead acetate (3, 7, 10, 30, 70, 100, and 300 µM) to the bath. Changes in rate of stimulation (0.1-1.5 Hz), relative potentiation after pauses of 15, 30, and 60 s, effect of Ca2+ concentration (0.62, 1.25, and 2.5 mM), and the effect of isoproterenol (20 ng/mL) were determined before and after the addition of 100 µM Pb2+. Effects on contractile proteins were evaluated after caffeine treatment using tetanic stimulation (10 Hz) and measuring...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lead; Right ventricular strips; Contraction; Heart; Myosin ATPase activity.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900008
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