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| Registros recuperados: 49 | |
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| Yarzábal,A.; Avilán,L.; Hoelzl,K.; Muñoz,M. de; Puig,J.; Kansau,I.. |
| The interaction of plasminogen, tissue plasminogen activator (t-PA) and urokinase with a clinical strain of Helicobacter pylori was studied. Plasminogen bound to the surface of H. pylori cells in a concentration-dependent manner and could be activated to the enzymatic form, plasmin, by t-PA. Affinity chromatography assays revealed a plasminogen-binding protein of 58.9 kDa in water extracts of surface proteins. Surface-associated plasmin activity, detected with the chromogenic substrate CBS 00.65, was observed only when plasminogen and an exogenous activator were added to the cell suspension. The two physiologic plasminogen activators, t-PA and urokinase, were also shown to bind to and remain active on the surface of bacterial cells. epsilon-Aminocaproic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Plasminogen; T-PA; Urokinase; Plasminogen activation. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900004 |
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| Quintero Díaz,Myrna; Behar Hasday,Rosa; García Lima,Clara Elsa; Pupo Olivero,Daymela; Hernández Domínguez,Maité; Díaz Elías,Javier; Pérez Suárez,Frank. |
| IINTRODUCCIÓN: Aloe vera L. (sin. A. barbadensis L.) (aloe o sábila) es una planta con vastas propiedades medicinales. Los estudios preclínicos demostraron la acción antiulcerosa, sin efectos tóxicos, de extractos acuosos de hoja fresca. No se encontró ningún ensayo clínico en la literatura similar al del presente estudio. OBJETIVO: comparar el efecto de la formulación Aloe gel viscoso® (Aloe) y amoxicilina administrados a pacientes ambulatorios con úlcera duodenal y Helicobacter pylori positivo, por la prueba de ureasa, sobre la evolución endoscópica de la úlcera y sobre el comportamiento del Helicobacter pylori frente a un grupo control. MÉTODOS: los 2 grupos de Aloe recibieron 2 dosis diferentes (1,5 o 3,0 mg/kg de peso corporal) durante 6 semanas y... |
| Tipo: Journal article |
Palavras-chave: Aloe vera; Sábila; Amoxicilina; Úlcera duodenal; Helicobacter pylori. |
| Ano: 2009 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962009000400002 |
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| Cogo,Laura Lúcia; Monteiro,Cristina Leise Bastos; Miguel,Marilis Dallarmi; Miguel,Obdulio Gomes; Cunico,Miriam Machado; Ribeiro,Marcelo Lima; Camargo,Eloá Ramalho de; Kussen,Gislene Maria Botão; Nogueira,Keite da Silva; Costa,Libera Maria Dalla. |
| The antibacterial activity of plant extracts obtained from Bixa orellana L., Chamomilla recutita L., Ilex paraguariensis A. St.-Hil., Malva sylvestris L., Plantago major L. and Rheum rhaponticum L. has been evaluated against two reference strains and eleven clinical isolates of Helicobacter pylori. All the plant species chosen are used in popular Brazilian cuisine and folk medicine in the treatment of gastrointestinal disorders. Initial screening was made by the disk diffusion test and then minimum inhibitory concentration was determined by the agar dilution method. The results presented in this work demonstrated that among the plant preparations analyzed, B. orellana L., C. recutita L., I. paraguariensis A. St.-Hil. and M. sylvestris L. were capable of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Antibacterial activity; Plant extracts. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200007 |
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| Monteiro,Jessica Raquel Borges; Ardisson,Juliana Santa; Athaydes,Brena Ramos; Gonçalves,Rita de Cássia Ribeiro; Rodrigues,Ricardo Pereira; Kuster,Ricardo Machado; Kitagawa,Rodrigo Rezende. |
| Abstract Helicobacter pylori is a bacterium that reaches half of the world population and it’s recognized as the main cause of chronic gastritis and peptic ulcer. In this study, we evaluated the anti-H. pylori, antioxidant and immunomodulatory activities of the methanolic (MeOH) extract of Eugenia uniflora leaves and chemical profile. Anti-H. pylori activity was evaluated by spectrophotometric broth microdilution technique by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), in addition to the evaluation of the effect on the urease enzyme. The antioxidant activity was evaluated by capturing O2 •-, HOCl e NO• radicals. The immunomodulatory effect was evaluated on the cytokines TNF-α, IL-6 and on nitric... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eugenia uniflora; Helicobacter pylori; Antioxidant; Immunomodulation. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100424 |
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| Ogata,Silvio Kazuo; Gales,Ana Cristina; Kawakami,Elisabete. |
| Antimicrobial susceptibility testing for Helicobacter pylori is increasingly important due to resistance to the most used antimicrobials agents. Only agar dilution method is approved by CLSI, but it is difficult to perform routinely. We evaluated the reliability of E-test and disk diffusion comparing to agar dilution method on Helicobacter pylori antimicrobial susceptibility testing. Susceptibility testing was performed for amoxicillin, clarithromycin, furazolidone, metronidazole and tetracycline using E-test, disk-diffusion and agar dilution method in 77 consecutive Helicobacter pylori strains from dyspeptic children and adolescents. Resistance rates were: amoxicillin - 10.4%, 9% and 68.8%; clarithromycin - 19.5%, 20.8%, 36.3%; metronidazole - 40.2%33.7%,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; E-test; Disk diffusion; Susceptibility test. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400039 |
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| FAURO, C. A. M.; TESSARI, L. C. M.; SANTOS, N. R. F. dos; CORREIA, N. S.; BONACORSI, C.; BAILÃO, A. M.; BAEZA, L. C.; VERBISCK, N. V.; DONOFRIO, F. C.. |
| Numerous studies have shown that subinhibitory concentrations of antimicrobials can alter bacterial virulence factors. This study evaluates motility and biofilm formation by H. pylori 43504 grown in subinhibitory concentrations of amoxicillin (AMX), clarithromycin (CLA), or tetracycline (TET). For the swimming and swarming motility assays, H. pylori 43504 suspensions were prepared with the strain alone or with the strain in AMX, CLA, or TET at ½ MIC. Next, the media were incubated at 37 ºC, under microaerophilia. To assess biofilm formation in the presence of one of the antimicrobials at subinhibitory antimicrobial concentrations, bacterial suspensions (109 CFU/mL) were prepared in 2.5% FBS containing AMX, CLA, or TET at ½ MIC. After incubation for 10... |
| Tipo: Artigo de periódico |
Palavras-chave: Biofilme; Gastrite; Microrganismo; Bacterial motility; Biofilm; Gastritis; Helicobacter pylori; Microorganisms. |
| Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150751 |
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| Atta,A.M.; Rodrigues,M.Z.A.; Sousa,C.P.; Medeiros Júnior,M.; Sousa-Atta,M.L.B.. |
| Chronic idiopathic urticaria (CIU) is a dermatological syndrome, characterized by raised erythematous skin lesions, that affects 20% of the general population and has been associated with autoimmunity. However, some reports have also suggested a close relationship between CIU and Helicobacter pylori infection, which is endemic in developing countries and associated with chronic gastritis, peptic ulcer disease, and gastric carcinoma. In the present study, we investigated the occurrence of autoantibodies in sera from 23 CIU subjects infected with H. pylori and from 23 CIU subjects without this infection. The presence of anti-thyroid antibodies was determined by indirect hemagglutination assay and the presence of autoantibodies to IgE and C1INH was determined... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Chronic idiopathic urticaria; Autoimmunity; Autoantibodies; Helicobacter pylori. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100002 |
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| Cogo,Laura Lúcia; Monteiro,Cristina Leise Bastos; Nogueira,Keite da Silva; Palmeiro,Jussara Kasuko; Ribeiro,Marcelo Lima; Camargo,Eloá Ramalho de; Neves,Daniel Locatelli; Nascimento,Aguinaldo José do; Costa,Libera Maria Dalla. |
| Prevalence of H. pylori infection was determined using cultures of gastric biopsy samples of patients attended at the academic hospital of the Federal University of Paraná, Curitiba, Paraná, Brazil. Molecular methods were used to characterize the cagA and vacA genes from bacterial isolates associated with different diseases presented by patients. Out of a total of 81, forty-two gastric biopsy samples tested were positive for H. pylori, with a prevalence of 51.9%. No significant difference was found with regard to the gender (p=0.793) and age (p=0.183) of the patients. Genotype s1m1 vacA gene was found in 67% of the cases of peptic ulcer investigated (p=1.0), despite the limited number of patients with this disease (n=3). A correlation between the presence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Virulence genes; CagA; VacA. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400007 |
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| César,Ana Cristina Gobbo; Cury,Patrícia Maluf; Payão,Spencer Luiz Marques; Liberatore,Paula Rahal; Silva,Ana Elizabete. |
| Helicobacter pylori colonization is associated with chronic gastritis, peptic ulcers, intestinal metaplasia, adenocarcinoma and lymphoma of the stomach. The objective of this study was to compare the results of the routinely used histology with molecular diagnosis for the detection of H. pylori. Eighty samples from gastric lesions (chronic gastritis, atrophic gastritis, gastric ulcer, and intestinal metaplasia), 18 gastric adenocarcinoma and 10 normal mucosa H. pylori-negative (control) samples were obtained. All samples were examined histologically (hematoxylin-eosin and Giemsa staining), and PCR amplifications of the species-specific antigen gene (H3H4) and urease A gene segment (H5H6) of H. pylori were made, using the human gene CYP1A1 for DNA quality... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Gastric lesions; Adenocarcinoma; Histological diagnosis; PCR. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100003 |
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| Módena,José Luiz Proença; Acrani,Gustavo Olszanski; Micas,André Fernando Ditondo; Castro,Marly de; Silveira,Wanderley Dias da; Módena,José Luiz Pimenta; Oliveira,Ricardo Brandt de; Brocchi,Marcelo. |
| Helicobacter pylori is considered a significant agent in the development of various gastric diseases. However, the diseases caused by this bacterium are known as being multi-factorial, with the genotype, immune system and life habits of the host playing important roles in the establishment of the clinical outcome. Also, H. pylori exhibit a high degree of genetic variability, contributing to the complexity of the host-pathogen relationship. These observations, considered together with the widely-varying origins and social habits of the Brazilian population, lead us to speculate about the influence of these life habits on H. pylori infection and the clinical outcome. Therefore, in this study we evaluated the relationship between H. pylori infection and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Duodenal ulcer; Gastric ulcer; Gastritis; Life habits. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100020 |
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| Rasmussen,LT; Labio,RW de; C Neto,A; Silva,LC; Queiroz,VF; Smith,MAC; Payão,SLM. |
| Helicobacter pylori, a gram-negative bacterium, possesses two important virulence factors: the vacuolating toxin (vacA), and the cytotoxin-associated gene product (cagA). The aim of the present study was to evaluate the presence of H. pylori in the stomach and oral cavity of humans and compare the cagA and vacA genotypes of H. pylori found in different samples (stomach, saliva and dental plaque) from the same patient. Gastric biopsies, saliva and dental plaques were obtained from 62 dyspeptic adults. DNA was extracted and evaluated for the presence of H. pylori and the alleles cagA and vacA. Persons with gastritis had a higher frequency of H. pylori -positive samples in the stomach while positive samples from gastric biopsies were significantly correlated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; CagA; VacA; Dental plaque; Dyspeptic patient; Saliva. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200008 |
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| Rabelo-Gonçalves,Elizabeth M A; Nishimura,Nancy F; Murilo R Zeitune,José. |
| An experimental model for H. pylori infection was established by intragastrically challenging BALB/c mice with 1 ml (10(8) CFU/ml) of suspension for two consecutive days. Animals were divided into three groups. GA: mice inoculated with fresh bacteria; GB: mice inoculated with frozen bacteria, and GC: mice inoculated with brucella broth (control group). Animals were killed at 7, 14, 21, 28, 35 and 60 days pi and fragments of stomach and duodenum were collected, paraffin embedded and stained by hematoxylin-eosin and Giemsa. The results showed that challenged mice exhibited mild duodenitis and gastritis. In group GA, infiltration in the duodenum was lymphoplasmacytic until day 35; in group GB, it was lymphomonocytic for 60 days pi. In the stomach, H. pylori... |
| Tipo: Journal article |
Palavras-chave: BALB/c mouse; Experimental model; Helicobacter pylori. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100012 |
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| Pourakbari,Babak; Ghazi,Mona; Mahmoudi,Shima; Mamishi,Setareh; Azhdarkosh,Hossein; Najafi,Mehri; Kazemi,Bahram; Salavati,Ali; Mirsalehian,Akbar. |
| Although several invasive and noninvasive tests have been developed for the diagnosis of Helicobacter pylori infection, all of the tests have their limitations. We conducted a study to investigate and compare the suitability of rapid urease test (RUT), serology, histopathology and stool antigen tests with polymerase chain reaction (PCR) for detection of H. pylori, and correlate the diagnostic methods with PCR. Eighty nine patients (61 adults, 28 children) referred to the Firoozgar Hospital and Children Medical Center Hospital for diagnostic upper gastrointestinal endoscopy entered to the study and noninvasive tests such as immunoassay for serological antibodies against H. pylori and detection of its antigen in feces were measured. The biopsies were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Diagnosis; Invasive and noninvasive tests. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300020 |
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| Li,X.B.; Ge,Z.Z.; Chen,X.Y.; Liu,W.Z.. |
| Whether the regression of gastric metaplasia in the duodenum can be achieved after eradication of Helicobacter pylori is not clear. The aim of the present study was to investigate the relationship between H. pylori infection and gastric metaplasia in patients with endoscopic diffuse nodular duodenitis. Eighty-six patients with endoscopically confirmed nodular duodenitis and 40 control patients with normal duodenal appearance were investigated. The H. pylori-positive patients with duodenitis received anti-H. pylori triple therapy (20 mg omeprazole plus 250 mg clarithromycin and 400 mg metronidazole, all twice daily) for one week. A control endoscopy was performed 6 months after H. pylori treatment. The H. pylori-negative patients with duodenitis received 20... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nodular duodenitis; Helicobacter pylori; Gastric metaplasia. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700002 |
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| Zhang,F.; Bao,Z.J.; Shi,D.M.; Xiang,P.; Xiao,L.; Huang,Y.Q.; Zhang,G.S.; Yin,S.M.. |
| We aimed to evaluate the effectiveness and safety of bismuth-containing quadruple therapy plus postural change after dosing for Helicobacter pylori eradication in gastrectomized patients. We compared 76 gastric stump patients with H. pylori infection (GS group) with 50 non-gastrectomized H. pylori-positive patients who met the treatment indication (controls). The GS group was divided into GS group 1 and GS group 2. All groups were administered bismuth potassium citrate (220 mg), esomeprazole (20 mg), amoxicillin (1.0 g), and furazolidone (100 mg) twice daily for 14 days. GS group 1 maintained a left lateral horizontal position for 30 min after dosing. H. pylori was detected using rapid urease testing and histologic examination of gastric mucosa before and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Gastrectomy; Gastric stump; Eradication; Efficacy. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200702 |
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| Shukla,Sanket Kumar; Prasad,K.N; Tripathi,Aparna; Singh,Avinash; Saxena,Ashish; Ghoshal,Uday Chand; Krishnani,Narendra; Husain,Nuzhat. |
| Helicobacter pylori and Epstein-Barr virus (EBV) infections are common worldwide. Although H. pylori infection is a major factor in gastroduodenal diseases, its role in association with EBV infection is unknown. Objective: To study the association of H. pylori infection and EBV DNA load in patients with gastroduodenal diseases. Methods: Biopsy samples were collected from 200 adult patients [non-ulcer dyspepsia (NUD) 100, peptic ulcer disease (PUD) 50, gastric carcinoma (GC) 50] undergoing upper gastrointestinal endoscopy. H. pylori infection was diagnosed by rapid urease test, culture, histopathology, PCR and Q-PCR. EBV DNA was detected by non-polymorphic Epstein-Barr nuclear antigen-1 (EBNA-1) gene based Q-PCR. Results: In patients with GC and PUD, EBV... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epstein-Barr virus infections; Stomach neoplasms; Helicobacter pylori. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600014 |
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| Ramis,Ivy Bastos; Moraes,Ernani Pinho de; Fernandes,Márcia Silveira; Mendoza-Sassi,Raul; Rodrigues,Obirajara; Juliano,Carlos Renan Varela; Scaini,Carlos James; Silva,Pedro Eduardo Almeida da. |
| Helicobacter pylori infects nearly 50% of the world's population. This microorganism is accepted as the most important agent of gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma. Currently many diagnostic methods exist for detecting H. pylori, however they all have limitations, thus it is recommend a combination of at least two methods. The aim of this study was to evaluate diagnostic methods, such as in-house urease test, culture and Polymerase Chain Reaction (PCR), for the detection of the H. pylori in gastric biopsy specimens of 144 dyspeptic patients, using as gold standard the association between histology and rapid urease test. According to the gold standard used in this study, 48 (33.3%) patients were infected with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Histology; Culture; Urease test; Polymerase Chain Reaction. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300008 |
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| Registros recuperados: 49 | |
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