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Identification of genes from flat oyster Ostrea edulis as suitable housekeeping genes for quantitative real time PCR ArchiMer
Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Renault, Tristan.
Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence data is available for O. edulis in public databases. Subtracted cDNA libraries were constructed from the O. edulis haemocytes in order to identify genes involved in host reactions against the parasite and quantitative real time PCR assays were developed to study expression of these genes. In this context, identification of reference genes and study of their...
Tipo: Text Palavras-chave: Real time PCR; Housekeeping genes; Haemocytes; Ostrea edulis; Bonamia ostreae.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00018/12880/9829.pdf
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Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities Anais da ABC (AABC)
LANDRIEL,SOLEDAD CAMINATA; CASTILLO,JULIETA D.L.M.; TABOGA,OSCAR A.; FERRAROTTI,SUSANA A.; GOTTLIEB,ALEXANDRA M.; COSTA,HERNÁN.
Abstract: Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclodextrin glycosyltransferases; Housekeeping genes; Paenibacillus; 16S rRNA.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620
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Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp Genet. Mol. Biol.
Mo,Fei; Zhao,Jie; Liu,Na; Cao,Li-hua; Jiang,Shan-xiang.
Reference genes are commonly used for normalization of target gene expression during RT-qPCR analysis. However, no housekeeping genes or reference genes have been identified to be stable across different tissue types or under different experimental conditions. To identify the most suitable reference genes for RT-qPCR analysis of target gene expression in the hepatopancreas of crucian carp (Carassius auratus) under various conditions (sex, age, water temperature, and drug treatments), seven reference genes, including beta actin (ACTB), beta-2 microglobulin (B2M), embryonic elongation factor-1 alpha (EEF1A), glyceraldehyde phosphate dehydrogenase (GAPDH), alpha tubulin (TUBA), ribosomal protein l8 (RPL8) and glucose-6-phosphate dehydrogenase (G6PDH), were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene expression; Quantitative RT-PCR; Housekeeping genes; CYP4T; Crucian carp.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400005
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