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Identification of genes from flat oyster Ostrea edulis as suitable housekeeping genes for quantitative real time PCR ArchiMer
Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Renault, Tristan.
Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence data is available for O. edulis in public databases. Subtracted cDNA libraries were constructed from the O. edulis haemocytes in order to identify genes involved in host reactions against the parasite and quantitative real time PCR assays were developed to study expression of these genes. In this context, identification of reference genes and study of their...
Tipo: Text Palavras-chave: Real time PCR; Housekeeping genes; Haemocytes; Ostrea edulis; Bonamia ostreae.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00018/12880/9829.pdf
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Identification of reference genes for RT-qPCR analysis in peach genotypes with contrasting chilling requirements. Repositório Alice
MARINI, N.; BEVILACQUA, C. B.; BÜTTOW, M. V.; RASEIRA, M. do C. B.; BONOW, S..
bitstream/item/165259/1/Marcia-do-Carmo.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Prunus persica; Housekeeping genes; Gene expression; RT qPCR.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1077661
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Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities Anais da ABC (AABC)
LANDRIEL,SOLEDAD CAMINATA; CASTILLO,JULIETA D.L.M.; TABOGA,OSCAR A.; FERRAROTTI,SUSANA A.; GOTTLIEB,ALEXANDRA M.; COSTA,HERNÁN.
Abstract: Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclodextrin glycosyltransferases; Housekeeping genes; Paenibacillus; 16S rRNA.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620
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Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. Repositório Alice
NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L..
Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the expression level of reference gene(s) to normalize the expression level of the genes of interest. To identify soybean reference genes for use in studies of flooding stress, we compared 5 candidate reference genes (CRGs) with the NormFinder and GeNorm programs to select the best internal control. The expression stability of the CRGs was evaluated in root tissues from soybean...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Endogenous genes; Flooding; Gene expression; Housekeeping genes; Internal control genes.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1001497
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Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. Repositório Alice
NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N.; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Endogenous genes; Internal control genes; Flooding; Housekeeping genes; Gene expression.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/980668
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Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. Repositório Alice
NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L..
Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the expression level of reference gene(s) to normalize the expression level of the genes of interest. To identify soybean reference genes for use in studies of flooding stress, we compared 5 candidate reference genes (CRGs) with the NormFinder and GeNorm programs to select the best internal control. The expression stability of the CRGs was evaluated in root tissues from soybean...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Endogenous genes; Flooding; Gene expression; Housekeeping genes; Internal control genes.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/980784
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Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp Genet. Mol. Biol.
Mo,Fei; Zhao,Jie; Liu,Na; Cao,Li-hua; Jiang,Shan-xiang.
Reference genes are commonly used for normalization of target gene expression during RT-qPCR analysis. However, no housekeeping genes or reference genes have been identified to be stable across different tissue types or under different experimental conditions. To identify the most suitable reference genes for RT-qPCR analysis of target gene expression in the hepatopancreas of crucian carp (Carassius auratus) under various conditions (sex, age, water temperature, and drug treatments), seven reference genes, including beta actin (ACTB), beta-2 microglobulin (B2M), embryonic elongation factor-1 alpha (EEF1A), glyceraldehyde phosphate dehydrogenase (GAPDH), alpha tubulin (TUBA), ribosomal protein l8 (RPL8) and glucose-6-phosphate dehydrogenase (G6PDH), were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene expression; Quantitative RT-PCR; Housekeeping genes; CYP4T; Crucian carp.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400005
Registros recuperados: 7
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