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Registros recuperados: 7
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A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee BABT
Fujii,Simone; Ono,Elisabete Yurie Sataque; Ribeiro,Ricardo Marcelo Reche; Assunção,Fernanda Garcia Algarte; Takabayashi,Cássia Reika; Oliveira,Tereza Cristina Rocha Moreira de; Itano,Eiko Nakagawa; Ueno,Yoshio; Kawamura,Osamu; Hirooka,Elisa Yoko.
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti IgG (10³-fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic-ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ochratoxin; Immunoassay; HPLC; Monoclonal antibody; Coffee.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200020
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A SENSITIVE AND SPECIFIC IMMUNOASSAY FOR THE MEASUREMENT OF THE ANTIBODIES PRESENT IN HORSE ANTIVENOMS ENDOWED WITH THE CAPACITY TO BLOCK THE PHOSPHOLIPASE A2-DEPENDENT HEMOLYSIS INDUCED BY SNAKE VENOMS J. Venom. Anim. Toxins
ROCHA CAMPOS,A. C. M.; FREITAS,M. C. W. DE; YAMAGUCHI,I. K.; MARCELINO,J. R.; DIAS DA SILVA,W..
Phospholipase A2 (PLA2), a component of most snake venom toxins, cleaves 3-sn-phosphoglycerides releasing lysophosphatidyl-choline. The indirect quantitative assay method for PLA2 was standardized for specific antivenom titration in a fast and sensitive assay by the similarity with the hemolysis induced by PLA2 and by complement system in sheep erythrocytes. The curves obtained by plotting the degree of hemolysis against the doses of snake venom are concave to the abscissa axis following an equation similar to that previously described for the hemolysis induced by the C system. We observed that venoms of some Bothrops, Crotalus and Micrurus species contained around 1 x 10<img SRC="http:/img/fbpe/jvat/v2n2/image603.gif"> to 10<img...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PLA2; Snake venoms; Immunoassay; Antivenoms; Indirect hemolysis.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301996000200004
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Analytical validation of anti-toxoplasma IgG immunoassays BJID
Souza,Guenael Freire de; Carvalho,Darlene; Pedrosa,William; Franck,Jacqueline; Piarroux,Renaud.
There are often discrepancies when using different methods to measure anti-Toxoplasma gondii IgG levels in patient samples. The diagnostic performance of a chemiluminescent immunoassay (CLIA) and an enzyme-linked fluorescent assay (ELFA) used as confirmatory tests for samples identified as positive or equivocal by an electrochemiluminescent immunoassay (ECLIA) were examined. Cut-off values were those stated by the manufacturer, and Western blot was used to confirm the results of all methods. All samples identified as positive by ECLIA (n = 93) were confirmed as positive by Western blot, as were 14 of the 28 samples identified as equivocal. When these 121 samples were retested, the sensitivities of CLIA and ELFA were 64.4% and 73.8%, respectively. Both...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Toxoplasma; Immunoassay; ECLIA; ELFA.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600012
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Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients BJM
El-Emshaty,Wafaa M; Raafat,Douaa; Elghannam,Doaa M; Saudy,Niveen; Eltoraby,Ehab E; Metwalli,Abd Elhameed A.
Nosocomial transmission of HCV is a concern in haemodialysis (HD) units worldwide. Diagnosis of HCV infection among dialysis patients is currently based on the detection of anti HCV antibodies by ELISA, and is confirmed by HCV RNA. The average window period between HCV infection and seroconversion with new generations of HCV antibody tests remains approximately 70 days with more prolonged period among dialysis patients. In this study we assessed the diagnostic performance of an immunoassay designed for simultaneous detection of anti HCV antibodies and core antigen in one step in comparison to qualitative RT-PCR and anti HCV antibodies detection test among Egyptian haemodialysis patients. The studied patients were 39 chronic renal failure patients on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Egypt; Haemodialysis; HCV; HCV core antigen; Immunoassay.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100039
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Immunological assays employed for the elucidation of an histoplasmosis outbreak in São Paulo, SP BJM
Passos,Angela Noronha; Kohara,Valdelene Sayuri; Freitas,Roseli Santos de; Vicentini,Adriana Pardini.
Several reports showed outbreaks of histoplasmosis acquired while bat-inhabited caves were visited by tourists, miners or researchers. We evaluated the performance of double immunodifusion (DI) and immunoblotting (IB) assays, employed for the histoplasmosis outbreak elucidation occurred in Vale do Paraíba, São Paulo. The existence of epidemiologic link, four patients with clinical signs suggestive of histoplasmosis and mycological confirmation has made that all 35 individuals involved to the cave visit were subjected to serological evaluation. By DI, we observed reactivity against H. capsulatum antigen in a single serum examined nearly 20 days after exposure to fungal propagules. On the other hand, IB showed reactivity against H and M fractions in 50% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Histoplasma; Histoplasmosis; Immunoassay; Double immunodiffusion; Immunoblotting.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400028
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Imunodiagnóstico para a artrite-encefalite caprina em rebanhos do semiárido baiano, Brasil. Repositório Alice
LIMA, C. C. V. de; COSTA, J. N.; SOUZA, T. S. de; MARTINEZ, P. M.; COSTA NETO, A. O.; AZEVEDO, D. A. A.; PINHEIRO, R. R.; BRITO, R. L. L. de..
Resumo: Este trabalho teve como finalidade padronizar a técnica de ensaio imunoenzimático indireto (Elisa-i) para diagnóstico da artrite-encefalite caprina (CAE) em rebanhos da Microrregião de Juazeiro, bem como comparar os resultados obtidos com outras técnicas imunodiagnósticas. Para tal, foram avaliadas 693 amostras de soros sanguíneos de caprinos, de 46 propriedades rurais da Microrregião (Campo Alegre de Lourdes, Casa Nova, Curaçá, Juazeiro, Pilão Arcado, Remanso, Sento Sé e Sobradinho). Quanto à soroprevalência obtida no Elisa-i, 1,59% (11/693) dos animais apresentaram anticorpos contra o vírus da CAE e 15,22% (7/46) das propriedades possuíram animal soropositivo. Quando na realização do IDGA comercial, apenas 0,29% (2/693) das amostras foram...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Técnica imunológica; CAEV; Imunodiagnóstico; Artrite-encefalite caprina; CAE; SRLV; Lentivírus; IDGA; Ensaio imunoenzimático; Caprino; Doença animal; Sorologia; Imunologia; Goats; Serology; Immunoassay; Immunology; ELISA.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/972707
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Survey of mycotoxins in Southern Brazilian wheat and evaluation of immunoassay methods. Repositório Alice
DUFFECK, M. R.; TIBOLA, C. S.; GUARIENTI, E. M.; DEL PONTE, E. M..
2017
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Triticum aestivum; Trigo; Micotoxina; Elisa; Triticum aestivum; Wheat; Mycotoxins; Immunoassay; Trichothecenes; Ochratoxins; Enzyme-linked immunosorbent assay.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1069840
Registros recuperados: 7
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