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A comparison of two agar gel immunodiffusion methods and a complement fixation test for serologic diagnosis of Brucella ovis infection in experimentally infected rams Arq. Bras. Med. Vet. Zootec.
Xavier,M.N.; Sant'Anna,F.M.; Silva,T.M.A.; Costa,E.A.; Moustacas,V.S.; Merlo,F.A.; Carvalho Júnior,C.A.; Dasso,M.G.; Mathias,L.A.; Gouveia,A.M.G.; Lage,A.P.; Santos,R.L..
A infecção por Brucella ovis é considerada uma das principais causas de epididimite e infertilidade em carneiros, resultando em falhas reprodutivas e perdas econômicas significativas em rebanhos ovinos ao redor do mundo. O estudo teve o objetivo de avaliar três testes sorológicos disponíveis para o diagnóstico da brucelose ovina por B. ovis, utilizando 181 soros ovinos. Amostras de soro provenientes de carneiros experimentalmente infectados foram coletadas ao longo de 192 dias pós-infecção (n=117) e durante o período pré-infecção (n=9). Adicionalmente, amostras de soro foram obtidas de ovinos provenientes de um rebanho livre para B. ovis (n=55). As técnicas de imunodifusão em gel de agar (IDGA), utilizando dois antígenos disponíveis comercialmente, e de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ram; Brucella ovis; Serology; Complement fixation; Immunodiffusion.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400031
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Development of a polymerase chain reaction and its comparison with agar gel immunodiffusion test in the detection of bovine leukemia virus infection Braz. J. Vet. Res. Anim. Sci.
Camargos,Marcelo Fernandes; Stancek,Daniel; Lessa,Leandro Moreira; Reis,Jenner Karlisson Pimenta; Rocha,Maurílio Andrade; Leite,Rômulo Cerqueira.
Polymerase chain reaction (PCR) was used for bovine leukemia virus (BLV) detection in the peripheral leukocytes of the infected bovines. The primers used were designed to amplify a part of env gene of BLV. PCR products were analyzed by agarose gel electrophoresis stained by ethidium bromide. The analytical specificity of PCR was confirmed by enzymatic restriction analysis of the PCR product with Bam HI and also by nucleotide sequence analysis of three PCR samples. Sixty five animals were tested for anti-BLV antibody, by agar gel-immunodiffusion test (AGID) and for direct BLV detection by PCR. There was a 73.80% concordance rate between the two tests. Four animals positive in AGID were PCR negative, while 13 AGID negative animals were found PCR positive....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunodiffusion; Bovine leukemia virus; Gene; Polymerase chain reaction.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000500005
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Prevalência da infecção por lentivírus de pequenos ruminantes em caprinos em Teresina, Piauí Arq. Bras. Med. Vet. Zootec.
Sampaio Júnior,A.; Batista,M.C.S.; Cruz,M.S.P.; Silva,R.A.B.; Bona Nascimento,C.; Werneck,G.L..
The prevalence of anti-lentiviruses antibodies of small ruminants was investigated in goat herds in the city of Teresina, PI, Brazil. A seroepidemiological survey was conducted involving 480 animals, apparently healthy, belonging to six rural properties. The diagnostic test was the agar gel immunodiffusion (AGID), using antigens produced from cellular cultures infected with caprine arthritis encephalitis virus (CAEV Cork). Prevalences by gender and age were estimated considering sampling fractions for each farm. A general prevalence of 4.2%, was observerved, being 4.2% for females and 3.6% for males. Prevalences were higher among older goats. Regarding the breed standard, 23.5% were of the Anglo Nubian, 5.9% of the Boer, 35.3% Anglo Nubian x Boer...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Caprine arthritis encephalitis virus; Transmission; Epidemiology; Immunodiffusion.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300032
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SEROLOGICAL DIAGNOSIS OF EQUINE INFECTIOUS ANEMIA VÍRUS INFECTION IN THE CENTRAL REGION OF THE RIO GRANDE DO SUL STATE Ciência Rural
Rebelatto,Marlon Cezar; Oliveira,Clóvis de; Weiblen,Rudi; Silva,Saul Fontoura da; Oliveira,Luiz Sérgio Segala de.
This paper consists of a brief review on equine Infectious anemia added of the results of serological diagnosis of this infection performed at the Federal University of Santa Maria, RS, Brazil, between 1979 to 1990. A total of 7,035 serum samples were tested by the agar gel immunodiffusion test (Coggins test), to which 40 (0,57%) reacted positively. This percentage of positivity is lower than in other regions of Brazil, probably due to the lack of predisposing factors for equine infectious anemia virus spread, such as, a low density of blood-sucking insects in the South of Brazil and the seldom use of massive therapies and vaccinations in the region.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Equine infectious anemia; Diagnosis; Immunodiffusion.
Ano: 1992 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781992000200012
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Use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis J. Venom. Anim. Toxins incl. Trop. Dis.
Silva,D. F.; Assis,C. M.; Zamboni,I. M.; Barreto,L. C.; Kohara,V. S.; Vicentini-Moreira,A. P..
The purpose of this work was to evaluate two serological assays: double immunodiffusion (DI) and immunoblotting (IB) in immunodiagnosis of paracoccidioidomycosis (PCM). We evaluated by IB assay 23 sera samples from patients with clinical confirmation of PCM, all of them with negative DI results against culture filtrate from Paracoccidioides brasiliensis isolate 113. For IB, as well as for comparative DI assay, we employed soluble components of the cell wall outer surface (SCCWOS) from P. brasiliensis isolate 113 cultivated at 36°C in Fava-Neto's agar medium for 5 and 10 days. Among the 20 sera samples analyzed by DI, 13 (65%) were negative and 7 (35%) were positive against SCCWOS obtained on the 5th and 10th days. By IB assay, 95.4% and 100% of sera...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paracoccidioidomycosis; Immunodiffusion; Immunoblotting.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200009
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