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| Michell,BC; Gomes,AD; Baião,NC; Resende,M; Lara,LJC; Martins,NRS. |
| The interference of low or high maternal antibodies titers on the attenuated infectious bursal disease (IBD) virus (IBDV) vaccine infection and its effects on the performance of broilers vaccinated at the 18th day of incubation (in ovo), at one day of age (subcutaneously-SC), or at 15 days of age (drinking water-DW) were investigated. After a series of three live vaccinations, breeders were given or not an IBD oil emulsion vaccine (IBD-OEV) prior to sexual maturity. At day 18 of incubation (in ovo), a commercial vaccine containing HVT and an intermediate IBDV strain or the single HVT vaccine was given. An intermediate IBDV vaccine was given SC at one day of age, or at 15 days of age via DW. The progeny of unvaccinated breeders presented higher neutralizing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Attenuated IBDV; Attenuated IBDV neutralization; Broiler chicken; Infectious bursal disease; Infectious bursal disease virus; IBDV; In-ovo vaccination; Maternal antibody; RT-PCR; Serum neutralization; Vaccination versus performance. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100009 |
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| Silva Jr.,J.V.J.; Arenhart,S.; Santos,H.F.; Almeida-Queiroz,S.R.; Silva,A.N.M.R.; Trevisol,I.M.; Bertani,G.R.; Gil,L.H.V.G.. |
| The Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time, however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro ligation and restriction sites. The aim of this research was to build the world's first VRG for IBDV by yeast-based homologous recombination; a more efficient, robust and simple process than cloning by in vitro ligation. The wild type IBDV (Wt-IBDV-Br) was isolated in Brazil and had its genome cloned in pJG-CMV-HDR vector by yeast-based homologous recombination. The clones were transfected into chicken... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infectious bursal disease virus; Reverse genetics; Yeast-based homologous recombination. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400054 |
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| Paula,Márcia Beatriz Cardoso de; Yokosawa,Jonny; Coutinho,Márcio Danilo Botrel; Silva,Paulo Lourenço; Ferraz,Rogério Avelar; Oliveira,Thelma Fátima Mattos; Queiróz,Divina Aparecida Oliveira. |
| In order to identify and characterize the agent of a suggestive clinical case of Gumboro disease (GD) that affected a 34-day-old broiler flock in Buriti Alegre (Goias State, Midwestern Brazil) in the year 2001, we carried out a combination of classic and modern virological methods. Histopathological analysis of the bursa revealed necrosis, presence of depleted follicles, some infiltration of heterophils, edema and formation of cystic cavities that are compatible with lesions observed in GD. Inoculation of embryonated eggs of specific pathogen-free (SPF) chickens with macerated bursa suspension resulted in embryo mortality and lesions which were also compatible with those caused by IBDV. A sample of bursa was submitted to a nested reverse... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken broiler flock; Gumboro disease; Infectious bursal disease virus; IBDV; RT-PCR; Molecular characterization. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300016 |
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| Nunes,Cristina Freitas; Fonseca,Fabiane; Leite,Alice Teixeira Meirelles; Silva Filho,Rodolfo Pinho da; Finger,Paula Fonseca; Castro,Clarissa Caetano; Fischer,Geferson; Vargas,Gilberto D'avila; Hübner,Silvia de Oliveira. |
| To investigate the exposure of the Newcastle disease virus (NDV), infectious bursal disease virus (IBDV) and avian poxvirus (APV) in Magellanic penguins found on the beaches in Southern regions of Brazil, the frequency of serum antibodies was estimated in 89 samples taken during 2005 and 2006. All the penguins were negative for the presence of antibodies against NDV by hemagglutination inhibition test and to APV by indirect ELISA. The reactivity was similar to the positives controls using ELISA kit for the IBDV made in the chickens in 50 samples. This reactivity also was demonstrated in 42 samples using agar gel immunodiffusion. No clinical signs related to IBDV infection were observed. The results indicated the absence of infection by NDV and APV but... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Magellanic penguins; Antibodies; Newcastle disease virus; Infectious bursal disease virus; Avian poxvirus. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400008 |
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| Simoni,Isabela Cristina; Fernandes,Maria Judite Bittencourt; Custódio,Renata Marconi; Madeira,Alda Maria Backx Noronha; Arns,Clarice Weis. |
| The susceptibility of the five cell lines - IB-RS-2, RK-13, Vero, BHK-21, CER - to reovirus S1133 and infectious bursal disease virus (IBDV vaccine GBV-8 strain) was studied to better define satisfactory and sensitive cell culture systems. Cultures were compared for presence of CPE, virus titers and detection of viral RNA. CPE and viral RNA were detected in CER and BHK-21 cells after reovirus inoculation and in RK-13 cell line after IBDV inoculation and with high virus titers. Virus replication by production of low virus titers occurred in IB-RS-2 and Vero cells with reovirus and in BHK-21 cell line with IBDV. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reovirus; Infectious bursal disease virus; Cell lines; Susceptibility. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400015 |
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