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Registros recuperados: 23
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A possible relationship between gluconeogenesis and glycogen metabolism in rabbits during myocardial ischemia 42
AGUIAR,RAQUEL R. DE; VALE,DANIELA F.; SILVA,RENATO M. DA; MUNIZ,YOLANDA P.; ANTUNES,FERNANDA; LOGULLO,CARLOS; OLIVEIRA,ANDRÉ L.A.; ALMEIDA,ADRIANA J. DE.
ABSTRACT Ischemia is responsible for many metabolic abnormalities in the heart, causing changes in organ function. One of modifications occurring in the ischemic cell is changing from aerobic to anaerobic metabolism. This change causes the predominance of the use of carbohydrates as an energy substrate instead of lipids. In this case, the glycogen is essential to the maintenance of heart energy intake, being an important reserve to resist the stress caused by hypoxia, using glycolysis and lactic acid fermentation. In order to study the glucose anaerobic pathways utilization and understand the metabolic adaptations, New Zealand white rabbits were subjected to ischemia caused by Inflow occlusion technique. The animals were monitored during surgery by pH and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene expression; Glycogen; Ischemia; Myocardium; Rabbit.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401683
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An acid-sensing ion channel that detects ischemic pain 56
Naves,L.A.; McCleskey,E.W..
Ischemic pain occurs when there is insufficient blood flow for the metabolic needs of an organ. The pain of a heart attack is the prototypical example. Multiple compounds released from ischemic muscle likely contribute to this pain by acting on sensory neurons that innervate muscle. One such compound is lactic acid. Here, we show that ASIC3 (acid-sensing ion channel #3) has the appropriate expression pattern and physical properties to be the detector of this lactic acid. In rats, it is expressed only in sensory neurons and then only on a minority (~40%) of these. Nevertheless, it is expressed at extremely high levels on virtually all dorsal root ganglion sensory neurons that innervate the heart. It is extraordinarily sensitive to protons (Hill slope 4,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pain; Ischemia; Dorsal root ganglia; Acid-sensing ion channels; Lactic acidosis.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100001
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Bone marrow mesenchymal stem cells overexpressing human basic fibroblast growth factor increase vasculogenesis in ischemic rats 56
Zhang,J.C.; Zheng,G.F.; Wu,L.; Ou Yang,L.Y.; Li,W.X..
Administration or expression of growth factors, as well as implantation of autologous bone marrow cells, promote in vivo angiogenesis. This study investigated the angiogenic potential of combining both approaches through the allogenic transplantation of bone marrow-derived mesenchymal stem cells (MSCs) expressing human basic fibroblast growth factor (hbFGF). After establishing a hind limb ischemia model in Sprague Dawley rats, the animals were randomly divided into four treatment groups: MSCs expressing green fluorescent protein (GFP-MSC), MSCs expressing hbFGF (hbFGF-MSC), MSC controls, and phosphate-buffered saline (PBS) controls. After 2 weeks, MSC survival and differentiation, hbFGF and vascular endothelial growth factor (VEGF) expression, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiogenesis; Basic fibroblast growth factor; Bone marrow mesenchymal stem cells; Gene transfection; Ischemia.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000886
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Effects of Ischemia/Reperfusion on ß Cells of Pancreas and Protective Effects of Melatonin Treatment 77
Yildirim,Ayse  ; Tuncer,Mehmet Cudi; Pamukçu,Özlem  ; Aktas  ,Ayfer  ; Akkus,Murat  .
Oxygen free radicals are considered to be important components involved in the pathophysiological tissue alterations observed during ischemia-reperfusion (I/R). In this study, we investigated the putative protective effects of melatonin treatment on pancreatic I/R injury. Sprague Dawley male rats were subjected to 30 min of pancreatic pedicle occlusion followed by 90 min reperfusion. Melatonin (10 mg/kg. s.c) was administrated 30 min prior to ischemia or I/R application. At the end of the reperfusion periods, rats were decapitated. Pancreatic samples were taken for transmission electron microscopy. The results indicated that ischemia created b cell damage as evidenced by dilatation between the nucleus inner and outer membrane and degeneration on islets of...
Tipo: Journal article Palavras-chave: Pancreas; SS cells; Melatonin; Ischemia; Reperfusion.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200036
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Epilepsy-induced electrocardiographic alterations following cardiac ischemia and reperfusion in rats 56
Tavares,J.G.P.; Vasques,E.R.; Arida,R.M.; Cavalheiro,E.A.; Cabral,F.R.; Torres,L.B.; Menezes-Rodrigues,F.S.; Jurkiewicz,A.; Caricati-Neto,A.; Godoy,C.M.G.; Gomes da Silva,S..
The present study evaluated electrocardiographic alterations in rats with epilepsy submitted to an acute myocardial infarction (AMI) model induced by cardiac ischemia and reperfusion. Rats were randomly divided into two groups: control (n=12) and epilepsy (n=14). It was found that rats with epilepsy presented a significant reduction in atrioventricular block incidence following the ischemia and reperfusion procedure. In addition, significant alterations were observed in electrocardiogram intervals during the stabilization, ischemia, and reperfusion periods of rats with epilepsy compared to control rats. It was noted that rats with epilepsy presented a significant increase in the QRS interval during the stabilization period in relation to control rats...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epilepsy; Heart; Acute myocardial infarction; Ischemia; Reperfusion.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200140
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Estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemia 48
TIIDUS,PETER M; DELLER,MIRADA; BOMBARDIER,ERIC; GÜL,MUSTAFA; LIU,X. LINDA.
This study examined the effects of estrogen supplementation on markers of neutrophil infiltration and damage in skeletal muscle of rats following ischemia. Male and female gonad-intact rats, with or without 14 days of estrogen supplementation were subjected to two hours of hind-limb ischemia and sacrificed at 24, 48 or 72 hours post-ischemia. Control animals were sacrificed without ischemia. Plantaris and red and white gastrocneimus muscles were removed and assayed for myeloperoxidase (MPO), a marker of neutrophil infiltration, and glucose-6-phosphate dehydrogenase (G6PD) and ß-glucuronidase (<img border=0 width=8 height=15 id="_x0000_i1026" src="http://fbpe/img/bres/v38n2-3/existe.gif" align=absbottom>GLU), as markers of muscle damage. Significant...
Tipo: Journal article Palavras-chave: Ischemia; Estrogen; Muscle damage; Skeletal muscle; Neutrophils.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200011
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Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on Regions 77
Rosero-Salazar,Doris Haydee.
Different situations cause ischemia and reperfusion injury, affecting tissues under the level of compression. In this research, abnormal characteristics in distribution of muscle fibers types in soleus and extensor carpi radialis longus, during short periods of ischemia and short and long periods of reperfusion, were determined. Fibers were classified by enzyme histochemistry techniques NADH-TR and myosin-ATPase. Measurements of areas were carried out through semiautomatic image processing by using segmentation based on regions, which evidenced significant changes in distribution during reperfusion followed to one and three hours of ischemia, as well as in comparisons of areas for all periods of reperfusion. This study strengthens the evidence about using...
Tipo: Journal article Palavras-chave: Histochemistry; Ischemia; Reperfusion; Image analysis; Segmentation; Histoquímica; Isquemia; Reperfusión; Análisis de imágenes; Segmentación.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100019
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Induction of chagasic-like arrhythmias in the isolated beating hearts of healthy rats perfused with Trypanosoma cruzi-conditioned medium 56
Rodríguez-Angulo,H.; Toro-Mendoza,J.; Marques,J.; Bonfante-Cabarcas,R.; Mijares,A..
Chagas' myocardiopathy, caused by the intracellular protozoan Trypanosoma cruzi, is characterized by microvascular alterations, heart failure and arrhythmias. Ischemia and arrythmogenesis have been attributed to proteins shed by the parasite, although this has not been fully demonstrated. The aim of the present investigation was to study the effect of substances shed by T. cruzi on ischemia/reperfusion-induced arrhythmias. We performed a triple ischemia-reperfusion (I/R) protocol whereby the isolated beating rat hearts were perfused with either Vero-control or Vero T. cruzi-infected conditioned medium during the different stages of ischemia and subsequently reperfused with Tyrode's solution. ECG and heart rate were recorded during the entire experiment. We...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chagas' disease; Arrhythmias; Ischemia.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100058
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Ischemic dermatopathy in a dog: case report 195
Fonterrada, Cláudia de Oliveira; Conceição, Lissandro Gonçalves.
Objective: To report on a case of ischemic dermatopathy, clinically and histopatologically compatible with multifocal ischemic dermatopathy. Description: A three-year old male Maltese dog was diagnosed With isquemic dermatopathy at Koala Hospital Animal (Koala Animal Hospital), São Paulo, Sp, Brazil. Initial lesions affected the pinnae,joolpads, scrolwn, hocks, olecranum, right flank, tip of the tail and periocular and perioral areas. The dog had been vaccinated against rabies in the right flank, four months before lesions were first noted. The patient was medicated with pentoxifylline and vitamin E, but lesions progressed despite treatment. Comments: It cannot be concluded that the treatment was totally inefficient, once the owner chose to discontinue...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Skin diseases; Vascular; Complications; Rabies vaccines; Adverse effects; Alopecia; Skin ulcer; Ischemia; Dogs Dermatopatías vasculares; Complicaciones; Vacunas contra la rabia; Efectos adversos; Alopecia; Úlcera cutánea; Isquemia; Perros dermatopatias vasculares; Complicações; Vacinas anti-rábicas; Efeitos adversos; Alopecia; Úlcera cutânea; Isquemia; Cães.
Ano: 2005 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3171
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Late cardiac perconditioning by phenylephrine in an isolated rat heart model is mediated by mitochondrial potassium channels 60
Nazari,Afshin; Sedighi,Mehrnoosh; Dalvand,Parvin; Azizi,Yaser; Moghimian,Maryam; Boroujeni,Shakiba Nasiri.
The present study was designed to investigate the effect of early and late administration of phenylephrine during ischemia against regional ischemia-reperfusion injuries in an isolated rat heart model. All animals were randomly divided into experimental groups: (I) IR (Ischemic/ reperfusion): the hearts underwent 35 min of regional ischemia followed by 60 min of reperfusion; (II) 5HD-IR-0: the hearts were perfused for 5 min with 5HD (5-hydroxydecanoate, specific mKATP channel blocker, 100 µM) at the onset of regional ischemia; (III) 5HD-IR-20: the hearts were perfused for 5 min with 5HD 20 min after regional ischemia; (IV) PE-IR-10: the hearts were perfused for 5 min with phenylephrine 10 min after regional ischemia; (V) PE-IR-30: the hearts were perfused...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenylephrine; Ischemia; Reperfusion; Preconditioning; Cardioprotection.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100561
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Mitochondrial K+ transport and cardiac protection during ischemia/reperfusion 56
Carreira,R.S.; Facundo,H.T.F.; Kowaltowski,A.J..
Mitochondrial ion transport, oxidative phosphorylation, redox balance, and physical integrity are key factors in tissue survival following potentially damaging conditions such as ischemia/reperfusion. Recent research has demonstrated that pharmacologically activated inner mitochondrial membrane ATP-sensitive K+ channels (mitoK ATP) are strongly cardioprotective under these conditions. Furthermore, mitoK ATP are physiologically activated during ischemic preconditioning, a procedure which protects against ischemic damage. In this review, we discuss mechanisms by which mitoK ATP may be activated during preconditioning and the mitochondrial and cellular consequences of this activation, focusing on end-effects which may promote ischemic protection. These...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mitochondria; Potassium; Calcium; Free radicals; Heart; Ischemia.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300004
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Periventricular leukomalacia in a neonatal calf 19
KOYAMA, Kenji; FUJITA, Riku; MAEZAWA, Masaki; FUKUMOTO, Natsuko; HORIUCHI, Noriyuki; INOKUMA, Hisashi; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 猪熊, 壽; 古林, 与志安.
A 10-day-old, Japanese Black, female calf had shown astasia since just after birth. Focal symmetrical periventricular malacic lesions of the cerebrum and suppurative arthritis of the left hip joint were observed in macroscopic examination. Histologically, the cerebral lesions were confirmed as periventricular leukomalacia (PVL). The location and histological features of the lesions were similar to PVL in humans, caused by neonatal ischemia/hypovolemia. This is the first report of PVL in a neonatal calf.
Palavras-chave: Ischemia; Neonatal calf; Periventricular leukomalacia.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4324
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Protective effects of centrally acting sympathomodulatory drugs on myocardial ischemia induced by sympathetic overactivity in rabbits 56
Catelli,M.; Feldman,J.; Bousquet,P.; Tibiriçá,E..
It is recognized that an imbalance of the autonomic nervous system is involved in the genesis of ventricular arrhythmia and sudden death during myocardial ischemia. In the present study we investigated the effects of clonidine and rilmenidine, two centrally acting sympathomodulatory drugs, on an experimental model of centrally induced sympathetic hyperactivity in pentobarbital-anesthetized New Zealand albino rabbits of either sex (2-3 kg, N = 89). We also compared the effects of clonidine and rilmenidine with those of propranolol, a ß-blocker, known to induce protective cardiovascular effects in patients with ischemic heart disease. Central sympathetic stimulation was achieved by intracerebroventricular injection of the excitatory amino acid L-glutamate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Autonomic nervous system; Antihypertensive agents; Antiarrhythmic agents; Ischemia; Sudden death.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100012
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Protective Effects of Geraniol on Long Term Renal Ischemia/Reperfusion Injury in Rats 52
Can,Senanur; Canbek,Mediha.
Abstract Possible protective effects of geraniol, known as antioxidant properties, were analyzed biochemically and histologically on experimental long-term renal ischemia/reperfusion I/R injury in rats. This study used 3-4 months old male Wistar albino rats and were divided into 4 groups (n = 7) by random selection: Group I (Sham Group), Group II (I/R+SF), Group III (I/R+50 mg/kg geraniol), and Group IV (I/R+100 mg/kg geraniol). A right nephrectomy was performed in all groups under anesthesia. Groups I and II were inoculated with SF (1 ml/kg) and Groups III and IV were inoculated with 50 mg/kg and 100 mg/kg of geraniol, injected intraperitoneally. For Groups II, III, and IV, I/R durations were determined to be 60 mins ischemia and 24 hours reperfusion. At...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ischemia; Reperfusion; Geraniol; Antioxidant; Protective effects; Rat.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100314
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Retinal protective effects of topically administered agmatine on ischemic ocular injury caused by transient occlusion of the ophthalmic artery 56
Hong,S.; Hara,H.; Shimazawa,M.; Hyakkoku,K.; Kim,C.Y.; Seong,G.J..
Agmatine, an endogenous polyamine and putative neuromodulator, is known to have neuroprotective effects on various neurons in the central nervous system. We determined whether or not topically administered agmatine could reduce ischemic retinal injury. Transient ocular ischemia was achieved by intraluminal occlusion of the middle cerebral artery of ddY mice (30-35 g) for 2 h, which is known to also induce occlusion of the ophthalmic artery. In the agmatine group (N = 6), a 1.0 mM agmatine-containing ophthalmic solution was administered four times daily for 2 weeks before occlusion. In the control group (N = 6), a 0.1% hyaluronic acid ophthalmic solution was instilled at the same times. At 22 h after reperfusion, the eyeballs were enucleated and the retinal...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Agmatine; Apoptosis; Ischemia; Neuroprotection; Retina.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300005
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Role of mitochondrial ATP-sensitive potassium channel-mediated PKC-ε in delayed protection against myocardial ischemia/reperfusion injury in isolated hearts of sevoflurane-preconditioned rats 56
Wang,C.; Hu,S.M.; Xie,H.; Qiao,S.G.; Liu,H.; Liu,C.F..
This study aimed to determine the role of mitochondrial adenosine triphosphate-sensitive potassium (mitoKATP) channels and protein kinase C (PKC)-ε in the delayed protective effects of sevoflurane preconditioning using Langendorff isolated heart perfusion models. Fifty-four isolated perfused rat hearts were randomly divided into 6 groups (n=9). The rats were exposed for 60 min to 2.5% sevoflurane (the second window of protection group, SWOP group) or 33% oxygen inhalation (I/R group) 24 h before coronary occlusion. The control group (CON) and the sevoflurane group (SEVO) group were exposed to 33% oxygen and 2.5% sevoflurane for 60 min, respectively, without coronary occlusion. The mitoKATP channel inhibitor 5-hydroxydecanoate (5-HD) was given 30 min before...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ischemia; Reperfusion; MitoKatp channel; Preconditioning; Protein kinase C.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600528
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Slaughterhouse blood as a perfusate for studying myocardial function under ischemic conditions 56
Bendjelid,K.; Canet,E.; Gasche,Y.; Andre-Fouet,X.; Revel,D.; Janier,M..
Metabolic studies using the in vitro non-recirculating blood-perfused isolated heart model require large volumes of blood. The present study was designed to determine whether heterologous pig blood collected from a slaughterhouse can be used as perfusate for isolated pig hearts perfused under aerobic and constant reduced flow conditions. Eight isolated working pig hearts perfused for 90 min at a constant flow of 1.5 ml g-1 min-1 with non-recirculated blood diluted with Krebs-Henseleit bicarbonate buffer at a hematocrit of 23% were compared to eight hearts subjected to the same protocol but perfused only with Krebs-Henseleit bicarbonate buffer solution. Hearts were paced at 100 bpm and subjected to aerobic perfusion at 38ºC. Hearts were weighed before...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Myocardium; Ischemia; Erythrocytes; Edema; Reperfusion injury.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100006
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Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerations 56
Petrucci Júnior,O.; Oliveira,P.P.M.; Carmo,M.R.; Vieira,R.W.; Braile,D.M..
In the present study we standardized an experimental model of parabiotic circulation of isolated pig heart. The isolated heart was perfused with arterial blood from a second animal as support and submitted to regional ischemia for 30 min, followed by total ischemia for 90 min and reperfusion for 90 min. Parameters for measurement of ventricular performance using different indices measured directly or indirectly from intraventricular pressure were defined as: maximum peak pressure, final diastolic pressure, pressure developed, first derivative of maximum pressure (dP/dt max), first derivative of minimum pressure (dP/dt min), systolic stress of the left ventricle (sigmas), and maximum elastance of the left ventricle. Isolated hearts subjected to regional and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isolated heart; Ischemia; Parabiotic circulation; Reperfusion; Model.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500013
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The decreased oxygen uptake during progressive exercise in ischemia-induced heart failure is due to reduced cardiac output rate 56
Rolim,N.P.L.; Mattos,K.C.; Brum,P.C.; Baldo,M.V.C.; Middlekauff,H.R.; Negrão,C.E..
We tested the hypothesis that the inability to increase cardiac output during exercise would explain the decreased rate of oxygen uptake (VO2) in recent onset, ischemia-induced heart failure rats. Nine normal control rats and 6 rats with ischemic heart failure were studied. Myocardial infarction was induced by coronary ligation. VO2 was measured during a ramp protocol test on a treadmill using a metabolic mask. Cardiac output was measured with a flow probe placed around the ascending aorta. Left ventricular end-diastolic pressure was higher in ischemic heart failure rats compared with normal control rats (17 ± 0.4 vs 8 ± 0.8 mmHg, P = 0.0001). Resting cardiac index (CI) tended to be lower in ischemic heart failure rats (P = 0.07). Resting heart rate (HR)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heart failure; Oxygen uptake rate; Cardiac index; Coronary ligation; Ischemia.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200018
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The Effects of Progesterone on Hypoxic Ischemic Injuries in the Cornu Ammonis (CA) Region of the Hippocampus of Neonatal Rats 77
Khan,Aaijaz Ahmed; Ramli,Norhida Binti; Ismail,Zulizhar Mohd.
Hypoxia-ischemia (HI) is a major cause of brain damage in the newborn. Several studies elicited the neuroprotective effects of progesterone in adult rats but there is very little literature available on neonatal rats. Therefore the present study is undertaken to see the effect of progesterone in hypoxic ischemic brain injury in neonatal rats, using an established neonatal HI rat pup model. Seven-day-old rat pups were subjected to right common carotid artery ligation and then 60 minutes hypoxia. The first dose of progesterone to treatment group was administered by peritoneal injection (4 mg/kg), after 10 minutes of exposure and subsequent doses were given by subcutaneous injection at 6 h, 24 h and 48 h intervals. Control group was also exposed to HI and was...
Tipo: Journal article Palavras-chave: Cornu Ammonis; Hippocampus; Hypoxia; Ischemia; Neonatal rats; Neuroprotective; Progestrone; Pyramidal cell.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300025
Registros recuperados: 23
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