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Registros recuperados: 5
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Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass Electron. J. Biotechnol.
Lee,Ki-Won; Choi,Gi Jun; Kim,Ki-Yong; Yoon,Sei Hyung; Ji,Hee Chung; Park,Hyung Soo; Lim,Young Chul; Lee,Sang-Hoon.
In the present study, genotypic variation of Agrobacterium-mediated transformation of Korean Italian ryegrass has been evaluated. Mature seed-derived calli of seven cultivars were inoculated and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA1301, which contains a reporter gene (gus) and a plant selectable marker gene conferring resistance to hygromycin (hpt) in the T-DNA region. The effects of several factors such as callus type and callus age on transformation effectiveness and the expression of the GUS gene were investigated. The highest transformation effectiveness (6.7%) was obtained with the Hwasan 101 cultivar when 9-week-old calli (type-I) were inoculated with Agrobacterium. The overall transformation rates of the...
Tipo: Journal article Palavras-chave: Agrobacterium; Forage crop; Italian ryegrass; Transgenic plants.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300008
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Partitioning of water soluble carbohydrates in vegetative tissues of Lolium multiflorum Lam. ssp. italicum cv. Lema Braz. J. Plant Physiol.
Sandrin,Carla Zuliani; Domingos,Marisa; Figueiredo-Ribeiro,Rita de Cássia Leone.
In temperate grasses, fructans are the major storage polysaccharides, being accumulated mainly in mature leaf sheaths, and also in the roots. The partitioning of carbohydrates within different organs regulates plant growth and development. The aim of the present work was to analyze the partitioning of water soluble carbohydrates in five different parts (elongating leaf blades, expanded leaf blades, upper and lower segments of the stubble, and roots) of plants of L. multiflorum cv. Lema, in order to contribute to an understanding of soluble carbohydrates distribution in these plants. Soluble carbohydrates and total fructose were analyzed in plants cultivated during 4 months in a glasshouse, by colorimetric, TLC and HPAEC-PAD techniques. Results showed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fructan; Grass; Italian ryegrass; Sugars.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200006
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Soil aggregation in a crop-livestock integration system under no-tillage Rev. Bras. Ciênc. Solo
Souza,Edicarlos Damacena de; Costa,Sérgio Ely Valadão Gigante de Andrade; Anghinoni,Ibanor; Carvalho,Paulo César de Faccio; Oliveira,Eric Victor Ferreira de; Martins,Amanda Posselt; Cao,Eduardo; Andrighetti,Marcelo.
Grazing intensities can influence soil aggregation, which can be temporarily and permanently affected. The objective of this study was to evaluate the aggregate stability in water at the end of a soybean cycle and during pasture development in a crop-livestock integration system under no-tillage and grazing intensities. The experiment was initiated in 2001, in a dystrophic Red Latosol, after soybean harvest. Treatments consisted of pasture (black oat + Italian ryegrass) at heights of 10, 20 and 40 cm, grazed by young cattle, and a control (no grazing), followed by soybean cultivation, in a randomized block design. Soil samples were collected at the end of the soybean cycle (May/2007), during animal grazing (September/2007) and at the end of the grazing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Black oat; Italian ryegrass; Mean weighted diameter; Soil structure.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000400033
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Stability in chromosome number and DNA content in synthetic tetraploids of Lolium multiflorum after two generations of selection Ciência Rural
Pereira,Roselaine Cristina; Santos,Natália de Souza; Bustamante,Fernanda de Oliveira; Mittelmann,Andrea; Techio,Vânia Helena.
ABSTRACT: Chromosome doubling of Italian ryegrass genotypes ( Lolium multiflorum Lam.) adapted to the brazilian edaphoclimatic conditions is an important strategy used by breeders and aims to obtain more vigorous genotypes with better forage quality and disease resistance. The effectiveness of chromosome doubling can be measured by genetic stability and fertility rates of plants over generations. However, a common problem in the polyploidization process is the regeneration of mixoploid plants that have impaired fertility and genetic stability. The objective of this study was to verify if progenies of recently tetraploidized plants remain stable regarding DNA content and chromosome number, over two generations. Progenies of L. multiflorum plants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosomal duplication; Mixoploidy; Italian ryegrass; Plant breeding.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200401
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Transcriptome analysis identifies candidate target genes involved in glyphosate-resistance mechanism in lolium multiflorum. Repositório Alice
CECHIN, J.; PIASECKI, C.; BENEMANN, D. P.; KREMER, F. S.; GALLI, V.; MAIA, L. C.; AGOSTINETTO, D.; VARGAS, L..
Italian ryegrass (Lolium multiflorum; LOLMU) is one of the most troublesome weeds in temperate regions in the world. This weed species interfere with wheat, corn, rye, and oat, causing significant crop yield losses. This species has evolved glyphosate resistance, making it difcult to control. The mechanisms of glyphosate resistance are still unknown, and an understanding thereof will favor the development of new strategies of management. The present study is the first transcriptome study in LOLMU using glyphosate-resistant and -sensitive biotypes, aiming to identify and to provide a list of the candidate target genes related to glyphosate resistance mechanism. The transcriptome was assembled de novo, producing 87,433 contigs with an N50 of 740 bp and an...
Tipo: Artigo de periódico Palavras-chave: Italian ryegrass; Next-generation sequencing; RNA-Seq; Diferential gene expression; Resistance mechanism.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129871
Registros recuperados: 5
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