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Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster: Biocell
Noher de Halac,I.; Cismondi,I.A.; Rodriguez-Garcia,M.I.; Famá,G..
Cell wall pectins are some of the most complex biopolymers known, and yet their functions remain largely mysterious. The aim of this paper was to deepen the study of the spatial pattern of pectin distribution in the aperture of Oenothera hookeri.velans ster/+ster fertile pollen. We used "in situ" immunocytochemical techniques at electron microscopy, involving monoclonal antibodies JIM5 and JIM7 directed against pectin epitopes in fertile pollen grains of Oenothera hookeri.velans ster/+ster. The same region was also analyzed by classical cytochemistry for polysaccharide detection. Immunogold labelling at the JIM7 epitope showed only in mature pollen labelling mainly located at the intine endo-aperture region. Cytoplasmic structures near the plasma membrane...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectins; Monoclonal antibodies; JIM5; JIM7; Immunogold; Pollen; Sporoderm; Oenothera; Cytochemistry.
Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100002
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