Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: 

RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 6
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
Detection of cucumber parthenogenic haploid embryos by floating of immature seeds in liquid medium National Institute of Agronomic Research
Lotfi, M.; Salehi, S..
Only a small percentage of seeds arisen by irradiated pollen include haploid embryo. Inspecting the seeds one by one for rescuing the embryos is a tedious and time consuming work which decreases haploid production efficiency in this method for cucurbit plants. Cucumber seeds formed three weeks after pollination with irradiated pollen were floated in liquid medium for about 10 days before excision of the embryos. This innovative method allowed a better development of haploid embryos; they turned to green and germinate like normal seeds and could be identified easily without the need of inspecting the seeds one by one. This method permitted an easier detection and extraction of embryos in comparison with common approach, increased the number and the quality...
Tipo: Poster Palavras-chave: Cucurbit; Cucumber; Irradiated pollen; Liquid culture; Parthenogenesis; Doubled haploid.
Ano: 2008 URL: http://hdl.handle.net/2174/234
Imagem não selecionada

Imprime registro no formato completo
High-yield production of scutellaria radix flavonoids (baicalein, baicalin and wogonin) by liquid-culture of Scutellaria baicalensis root-derived cells BABT
Ohtsuki,Takashi; Himeji,Mika; Fukazawa,Harumi; Tanaka,Miho; Yamamoto,Hisako; Mimura,Akio.
Production of baicalein, baicalin and wogonin by liquid culture of Scutellaria baicalensis cells derived from the plant root was studied. The maximum production obtained were 119 mg/L of baicalein at two week, 1372 mg/L of baicalin at eight week, and 14 mg/L of wogonin at two week. In addition, the production of baicalin was drastically increased to 1000 mg/L level at 3-week culture, and the extremely high production rate (339 mg/L•week) was obtained. In the comparison of total antioxidative activities among baicalein, baicalin and wogonin, evaluated by thiocyanate method, it was suggested that the location of hydroxyl groups both at 5- and 6-position contributed to enhancement of radical scavenging activity, and/or methoxylation at 8-position diminished...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Flavonoids; Liquid culture; High-yield production; Scutellaria baicalensis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200005
Imagem não selecionada

Imprime registro no formato completo
Human erythroid progenitors from adult bone marrow and cord blood in optimized liquid culture systems respectively maintained adult and neonatal characteristics of globin gene expression Biol. Res.
ZHANG,XIN; MA,YAN-NI; ZHANG,JUN-WU.
In vitro suspension culture procedures for erythroid progenitor cells make it possible for us to obtain large cultures of erythrocyte populations for the investigation of globin gene switching. In this study we aimed to establish optimized culture systems for neonatal and adult erythroblasts and to explore the globin expression patterns in these culture systems. To culture CD34+ cells purified from human umbilical cord blood (CB) and adult bone marrow (BM), we respectively replaced the fetal bovine serum (FBS) with human cord serum and human adult serum. These CD34+ cells were then induced to erythroid differentiation. All the globin mRNA (including á-, ξ-, β-, γ-and ε-globin), the hemoglobin (Hb)-producing erythroid...
Tipo: Journal article Palavras-chave: Adult bone marrow; Cord blood; Erythroid progenitor; Globins; Liquid culture.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100005
Imagem não selecionada

Imprime registro no formato completo
Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species Chilean J. Agric. Res.
Muñoz,Manuel; Seemann,Peter; Jara,Gloria; Riegel,Ricardo.
Rhodophiala C. Presl (Amaryllidaceae) is a genus of attractive flowering geophytes native to South America. They have ornamental value, but most species are little known and have conservation problems. The objective of this study was to optimize a micropropagation process to support the use and preservation of three Chilean native species, R. montana (Phil.) Traub, R. splendens (Rengifo) Traub., and R. ananuca (Phil.) Traub. The research evaluated the feasibility of implementing liquid medium culture and assessed the influence of different tissue culture systems on the shoot production and biomass increment of small bulbs. Three experiments were carried out. The first one determined the influences of flask size and volume of media; the second compared...
Tipo: Journal article Palavras-chave: TIS; Liquid culture; Micropropagation; Acclimatization.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400014
Imagem não selecionada

Imprime registro no formato completo
Lipid formation and γ-linolenic acid production by Mucor circinelloides and Rhizopus sp., grown on vegetable oil BJM
Tauk-Tornisielo,Sâmia M.; Arasato,Luciana S.; Almeida,Alex F. de; Govone,José S.; Malagutti,Eleni N..
The fungi strains were tested in Bioscreen automated system to select the best nutritional source. Following, shaking submserse cultures were studied in media containing sole carbon or nitrogen source. The growth of these strains improved in media containing vegetable oil, with high concentration of lipids. The high concentration of γ-linolenic acid was obtained with M. circinelloides in culture containing sesame oil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Automated system; Vegetable oils; Zigomycetes; Γ-linolenic acid; Liquid culture.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200025
Imagem não selecionada

Imprime registro no formato completo
The establishment of in?vitro culture and drug screening systems for a newly isolated strain of Trypanosoma equiperdum OAK
Suganuma, Keisuke; Yamasaki, Shino; Molefe, Nthatisi Innocentia; Musinguzi, Peter Simon; Davaasuren, Batdorj; Mossaad, Ehab; Narantsatsral, Sandagdorj; Battur, Banzragch; Battsetseg, Badgar; Inoue, Noboru.
Dourine is caused by Trypanosoma equiperdum via coitus with an infected horse. Although dourine is distributed in Equidae worldwide and is listed as an internationally important animal disease by the World Organization for Animal Health (OIE), no effective treatment strategies have been established. In addition, there are no reports on drug discovery, because no drug screening system exists for this parasite. A new T.?equiperdum strain was recently isolated from the genital organ of a stallion that showed typical symptoms of dourine. In the present study, we adapted T.?equiperdum IVM-t1 from soft agarose media to HMI-9 liquid media to develop a drug screening assay for T.?equiperdum. An intracellular ATP-based luciferase assay using CellTiter-Glo reagent...
Palavras-chave: Colorimetric assay; Drug screening system; Liquid culture; Luciferase assay; Trypanosoma equiperdum.
Ano: 2017 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4538
Registros recuperados: 6
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional