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Registros recuperados: 11
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A combined proteome and transcriptome analysis of developing Medicago truncatula seeds: evidence for metabolic specialization of maternal and filial tissues Inra
Gallardo, K.; Firnhaber, C.; Zuber, H.; Héricher, D.; Belghazi, M.; Henry, C.; Küster, H.; Thompson, R..
A comparative study of proteome and transcriptome changes during Medicago truncatula (cultivar Jemalong) seed development has been carried out. Transcript and protein profiles were parallel across the time course for 50% of the comparisons made, but divergent patterns were also observed, indicative of post-transcriptional events. These data, combined with the analysis of transcript and protein distribution in the isolated seed coat, endosperm, and embryo, demonstrated the major contribution made to the embryo by the surrounding tissues. First, a remarkable compartmentalization of enzymes involved in methionine biosynthesis between the seed tissues was revealed that may regulate the availability of sulfur-containing amino acids for embryo protein synthesis...
Tipo: Journal Article Palavras-chave: SEED DEVELOPMENT; MEDICAGO TRUNCATULA; METHIONINE; METABOLISM; AMINO ACID TRANSPORTERS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200774127ef2&uri=/notices/prodinra1/2010/09/
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Affinement du concept de la protéine idéale pour le porc en croissance Inra
Henry, Y..
Cet article traite des données récentes sur les relations d’équilibre entre les acides aminés dans l’alimentation du porc en croissance, au travers du concept de la protéine "idéale". Après avoir précisé les limites de ce concept, sur le plan de la constance des rapports entre les teneurs en acides aminés indispensables et celle en lysine, prise comme référence, sont considérés les ratios entre les acides aminés limitants secondaires (tryptophane, thréonine, méthionine et acides aminés soufrés) et la lysine, compte tenu des spécificités de leur rôle fonctionnel au plan métabolique et physiologique. Pour cela, l’incidence des écarts entre le profil de composition en acides aminés de la protéine alimentaire et celui de la protéine idéale (excès de protéines)...
Tipo: Journal Article Palavras-chave: EQUILIBRE EN ACIDES AMINES; TYROSINE; PORC EN CROISSANCE; SEROTONINE; LEUCINE; ISOLEUCINE; VALINE; PHENYLALANINE; LYSINE; TRYPTOPHANE; THREONINE; METHIONINE.
Ano: 1993 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9400026690041834&uri=/notices/prodinra1/2008/02/
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Crystal structures of cobalamin-independent methionine synthase complexed with zinc, homocysteine, and methyltetrahydrofolate Inra
Ferrer, J.L.; Ravanel, S.; Robert, M.; Dumas, R..
Cobalamin-independent methionine synthase (MetE) catalyzes the synthesis of methionine by a direct transfer of the methyl group of N5-methyltetrahydrofolate (CH3-H4PteGlun) to the sulfur atom of homocysteine (Hcy). We report here the first crystal structure of this metalloenzyme under different forms, free or complexed with the Hcy and folate substrates. The Arabidopsis thaliana MetE (AtMetE) crystals reveal a monomeric structure built by two (βα)8 barrels making a deep groove at their interface. The active site is located at the surface of the C-terminal domain, facing the large interdomain cleft. Inside the active site, His647, Cys649, and Cys733 are involved in zinc coordination, whereas Asp605, Ile437, and Ser439 interact with Hcy. Opposite the...
Tipo: Journal Article Palavras-chave: ARABIDOPSIS THALIANA; STRUCTURE TRIDIMENSIONNELLE; SITE ACTIF; HOMOCYSTEINE; FOLATE; SITE DE LIAISON; ZINC; METHIONINE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0500031912109810&uri=/notices/prodinra1/2010/11/
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Effects of the isopropylester of the hydroxylated analogue of methionine (HMBi) on production performance of dairy cows in early lactation Inra
Jurjanz, S.; Robert, J.C.; Laurent, F..
Thirty six Holstein cows (26 multiparous, 10 primiparous) were assigned to one of two treatments 17 to 24 days after calving. A basal diet consisting of (DM basis) 61.5 % corn silage, 6.7% wheat straw, 14.9% cracked wheat, 15.6% soybean meal, 1.3 % minerals was fed for 17 weeks as a control diet (treatment 1: T1), or was supplemented with 0.12 % of diet DM with HMBi (treatment 2: T2). The control diet contained an estimated (per kg DM) 1.61 Mcal Net Energy, 100g metabolizable protein (MP), 46g digestible undegraded protein (PDIA from PDI system), 6.91 metabolizable lysine (%MP) and 1.78 metabolizable methionine (%MP). Treatment 2 contained 2.25 metabolizable methionine (%MP), HMBi supplying 0.47 metabolizable methionine (%MP). Results were analysed through...
Tipo: Conference Paper Palavras-chave: VACHE LAITIERE; RATION; ALIMENTATION COMPLEMENTAIRE; ACIDE AMINE; METHIONINE; PROTEINE DU LAIT; METABOLISME DAIRY COW; MILK PROTEIN; PERFORMANCE; DIET; HMBI.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007f0cf496e&uri=/notices/prodinra1/2011/03/
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Enzyme-induced covalent modification of methionyl-tRNA synthetase from Bacillus stearothermophilus by methionyl-adenylate : identification of the labeled amino acid residues by matrix-assisted laser desorption-ionization mass spectrometry Inra
Hountondji, C.; Beauvallet, C.; Pernollet, J.C.; Blanquet, S..
Methionyl-tRNA synthetase (MetRS) from Bacillus stearothermophilus was shown to undergo covalent methionylation by a donor methionyl-adenylate, the mixed carboxylic-phosphoric acid anhydride synthesized by the enzyme itself. Covalent reaction of methionyl-adenylate with the synthetase or other proteins proceeds through the formation of an isopeptide bond between the carboxylate of the amino acid and the ε-NH2 group of lysyl residues. The stoichiometries of labeling, as followed by TCA precipitation, were 2.2 ± 0.1 and 4.3 ± 0.1 mol of [14C]Met incorporated by 1 mol of the monomeric MS534 and the native dimeric species of B. stearo methionyl-tRNA synthetase, respectively. Matrix-assisted laser desorption-ionization mass spectrometry designated lysines-261,...
Tipo: Journal Article Palavras-chave: SYNTHETASE D'ARN DE TRANSFERT; METHIONINE; EXTREMITE 3'; ADENYLATE METHIONYLE; LIEN ISOPEPTIDE; MALDI-MS; MODIFICATION POSTTRANSLATIONNELLE; SITE DE LIAISON; ORDRE DE SIGNATURE; LEVURE DE BOULANGER; ACTIVATION; MECANISME METHIONYL-tRNA SYNTHETASE; METHIONYL-ADENYLATE; ISOPEPTIDE BOND; MALDI-MS; POSTTRANSLATIONAL MODIFICATION.
Ano: 2000 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0600023845111614&uri=/notices/prodinra1/2010/12/
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Identification of six novel allosteric effectors of Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase isoforms. Physiological context sets the specificity Inra
Curien, G.; Ravanel, S.; Robert, M.; Dumas, R..
The Arabidopsis genome contains two genes predicted to code for bifunctional aspartate kinase-homoserine dehydrogenase enzymes (isoforms I and II). These two activities catalyze the first and the third steps toward the synthesis of the essential amino acids threonine, isoleucine, and methionine. We first characterized the kinetic and regulatory properties of the recombinant enzymes, showing that they mainly differ with respect to the inhibition of the homoserine dehydrogenase activity by threonine. A systematic search for other allosteric effectors allowed us to identify an additional inhibitor (leucine) and 5 activators (alanine, cysteine, isoleucine, serine, and valine) equally efficient on aspartate kinase I activity (4-fold activation). The six...
Tipo: Journal Article Palavras-chave: VOIE METABOLIQUE; ACIDE AMINE ESSENTIEL; ASPARTATE KINASE HOMOSERINE DEHYDROGENASE; ISOFORME; EFFECTEUR ALLOSTERIQUE; INHIBITION ENZYMATIQUE; THREONINE; ISOLEUCINE; METHIONINE; ACTIVITE ENZYMATIQUE.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500032200111312&uri=/notices/prodinra1/2010/11/
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Intérêts et limites d'un apport de méthionine et de lysine dans l'alimentation des vaches laitières. Inra
Rulquin, H..
L’objectif de cette étude analytique de la bibliographie a été de préciser les réponses des vaches laitières à un apport post-ruminal de méthionine et de lysine, et d’en déterminer les facteurs de variation. Cent vingt et un essais mesurant les effets de l’apport de ces acides aminés (AA) (sous forme d’infusion dans l’intestin ou d’acides aminés protégés contre les dégradations ruminales) sur la production et la composition du lait ont été utilisés. Globalement, ces AA élèvent la production de protéines en accroissant le taux protéique du lait. L’augmentation est plus importante lorsque les deux AA sont associés (+ 1,1 g/kg) que lorsqu’ils ne le sont pas (+ 0,4 g/kg). Lorsque les deux AA sont apportés ensemble, la réponse est plus importante avec les...
Tipo: Journal Article Palavras-chave: VACHE LAITIERE; METHIONINE; LYSINE.
Ano: 1992 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9200012110024011&uri=/notices/prodinra1/2008/03/
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Methionine catabolism in Arabidopsis cells is initiated by a gamma-cleavage process and leads to S-methylcysteine and isoleucine synthesis Inra
Rébeillé, F.; Jabrin, S.; Bligny, R.; Loizeau, K.; Gambonnet, B.; Van Wilder, V.; Douce, R.; Ravanel, S..
Despite recent progress in elucidating the regulation of methionine (Met) synthesis, little is known about the catabolism of this amino acid in plants. We present here several lines of evidence indicating that the cleavage of Met catalyzed by Met -lyase is the first step in this process. First, we cloned an Arabidopsis cDNA coding a functional Met -lyase (AtMGL), a cytosolic enzyme catalyzing the conversion of Met into methanethiol, α-ketobutyrate (αKB) and ammonia. AtMGL is present in all the Arabidopsis organs and tissues analyzed, except in quiescent dry mature seeds, thus suggesting that AtMGL is involved in the regulation of Met homeostasis in various situations. Also, we demonstrated that the expression of AtMGL was induced in Arabidopsis cells in...
Tipo: Journal Article Palavras-chave: PLANTE EXPERIMENTALE; SOUFRE; METHIONINE; RESONANCE MAGNETIQUE NUCLEAIRE; RMN METHIONINE GAMMA LYASE.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007f25a9ec8&uri=/notices/prodinra1/2008/02/
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Methionine metabolism in plants. Chloroplasts are autonomous for de novo methionine synthesis and can import s-adenosylmethionine from the cytosol Inra
Ravanel, S.; Block, M.A.; Rippert, P.; Jabrin, S.; Curien, G.; Rébeillé, F.; Douce, R..
The subcellular distribution of Met and S-adenosylmethionine (AdoMet) metabolism in plant cells discloses a complex partition between the cytosol and the organelles. In the present work we show that Arabidopsis contains three functional isoforms of vitamin B12-independent methionine synthase (MS), the enzyme that catalyzes the methylation of homocysteine to Met with 5-methyltetrahydrofolate as methyl group donor. One MS isoform is present in chloroplasts and is most likely required to methylate homocysteine that is synthesized de novo in this compartment. Thus, chloroplasts are autonomous and are the unique site for de novo Met synthesis in plant cells. The additional MS isoforms are present in the cytosol and are most probably involved in the regeneration...
Tipo: Journal Article Palavras-chave: ARABIDOPSIS THALIANA; PHYSIOLOGIE CELLULAIRE; METHIONINE-SYNTHASE; S-ADENOSYLMETHIONINE; LOCALISATION CELLULAIRE; METHIONINE; CHLOROPLASTE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400031395104830&uri=/notices/prodinra1/2010/11/
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Methionine transsulfuration is increased during sepsis in rats Inra
Malmezat, T.; Breuillé, D.; Pouyet, C.; Buffière, C.; Denis, P.; Patureau-Mirand, P.; Obled, C..
Tipo: Journal Article Palavras-chave: INFECTION; METHIONINE; CYSTEINE; ACTIVITE ENZYMATIQUE; DEFENSE DE L'ORGANISME.
Ano: 2000 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0100002852088523&uri=/notices/prodinra1/2010/09/
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Regeneration mechanisms of arabidopsis thaliana methionine sulfoxide reductases B by glutaredoxins and thioredoxins Inra
Tarrago, L.; Laugier, E.; Zaffagnini, M.; Marchand, C.; Le Maréchal, P.; Rouhier, N.; Lemaire, S.D.; Rey, P..
Methionine oxidation leads to the formation of S- and R-diastereomers of methionine sulfoxide (MetSO), which are reduced back to methionine by methionine sulfoxide reductases (MSRs) A and B, respectively.MSRBs are classified in two groups depending on the conservation of one or two redox-active Cys: 2-Cys MSRBs possess a catalytic Cys reducing MetSO and a resolving Cys allowing regeneration by thioredoxins (Trxs). The second type, 1-Cys MSRBs, possess only the catalytic Cys. The biochemical mechanisms involved in activity regeneration of 1-Cys MSRBs remain largely elusive. In the present work, we used recombinant plastidial Arabidopsis thaliana MSRB1 and MSRB2, as models for 1-Cys and 2-Cys MSRBs, respectively, to delineate the Trx- and...
Tipo: Journal Article Palavras-chave: ARABIDOPSIS THALIANA; METHIONINE; REGENERATION; ESCHERICHIA-COLI; BIOCHEMICAL-CHARACTERIZATION; S-GLUTATHIONYLATION; CATALYTIC MECHANISM; REDOX REGULATION; REDUCING AGENT; ACTIVE-SITE; PROTEIN; PEROXIREDOXIN; POPLAR.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010bd5e65f0&uri=/notices/prodinra1/2010/10/
Registros recuperados: 11
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