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| FONSECA, L. M.; ELOY, A. M. X.; PINHEIRO, R. R.; PINHEIRO, A. A.. |
| Na fase crônica da artrite encefalite caprina (CAE), as metaloproteinases de matriz (MMPs) são ativadas, por desequilíbrios homeostáticos no animal. Elas atuam na degradação e remodelamento extracelular, ocorrendo tanto em processos fisiológicos como patologias de caráter agudo ou crônico. As gelatinases (MMP-2 e MMP-9), em suas formas latente e ativa, terão foco neste trabalho. O objetivo foi identificar a atividade das metaloproteinases -2 e -9, através da Zimografia, no plasma sanguíneo (PS) de caprinos infectados com CAE. |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Zimografia; Artrite encefalite caprina; Matrix metalloproteínases; Metaloproteinase; MMPs; Zymography; Caprino; Atividade Enzimática; Lentivirus; Goat diseases; Goats; Animal diseases; Caprine arthritis-encephalitis; Enzyme activity; Brazil; Metalloproteinases. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120491 |
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| Palma,Heloisa Einloft; Gallio,Miguel; Silva,Gabriele Biavaschi da; Cantarelli,Camila; Bertolin,Kalyne; Wolkmer,Patrícia; Wergutz,Julien; Krause,Luciana Maria Fontanari; Krause,Alexandre; Antoniazzi,Alfredo Quites; Brass,Karin Erica; Corte,Flavio Desessards De La. |
| ABSTRACT: In healthy cartilage, chondrocytes maintain an expression of collagens and proteoglycans and are sensitive to growth factors and cytokines that either enhance or reduce type II collagen synthesis. In osteoarthritis, pro-inflammatory cytokines, such as IL-6, induce overexpression of metalloproteinases (MMP) and decreasing synthesis of aggrecan. Use of chondroprotectors agents, such as Platelet-Rich Plasma (PRP) and triamcinolone (TA) are alternatives to reduce the progression of joint damage. In this study, we used chondrocytes extracted from metacarpophalangeal joints of healthy horses as the experimental model. Cells were treated in vitro with PRP or TA. No differences were observed between these treatments in comparison to the control group... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MMPs; IL-6; Aggrecan; Cartilage; Osteoarthritis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700601 |
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| Lee,Eun-Gyeong; Lee,Sang-ll; Chae,Han-Jung; Park,Seoung Ju; Lee,Yong Chul; Yoo,Wan-Hee. |
| Objectives: The objective of this study is to determine the effects of Ethyl acetate fraction from Cudrania tricuspidata (EACT) on the interleukin-1b (IL-1b)-induced proliferation of rheumatoid synovial fbroblasts (RASFs) and production of matrix metalloproteinases (MMPs), cyclooxygenase (COX) and prostaglandin E2 (PGE2) by RASFs. Materials and Methods: The proliferation of RASFs was evaluated with CCK-8 reagent in the presence of IL-1b with/without EACT. The expression of MMPs, TIMP-1, COXs, PGE2 and intracellular MAPK signalings, including p-ERK, p-p38, p-JNK and NF-kB were examined by immunoblotting or semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and ELISA in conditions as described above. Results: EACT inhibits... |
| Tipo: Journal article |
Palavras-chave: Cudrania tricuspidata; COX; IL-1b; MMPs; PGE2; Rheumatoid Arthritis (RA). |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200010 |
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| Xu,Shuxiang; Fan,Yingying; Li,Dongping; Liu,Yan; Chen,Xu. |
| Cervical cancer is one of the most common cancers among women around the world. However, the underlying mechanism involved in cervical cancer progression is incompletely known. In the present study, we determined the role of glycoprotein nonmetastatic melanoma protein B (GPNMB) in tumorigenesis of cervical cancer. According to the GEO database, we found that GPNMB expression was significantly higher in cervical cancer than in normal cervix epithelium. A similar pattern was observed in GPNMB expression in cultured cervical cancer cells and normal cervical epithelial cells. Compared with the control, GPNMB knockdown significantly decreased the proliferation and migration capacity, but enhanced the apoptosis capacity of SiHa and HeLa cells. Additionally, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cervical cancer; GPNMB; Tumorigenesis; MMPs; Wnt/β-catenin. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100607 |
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| Zampini,Renato; Apichela,Silvana; Sari,Luciana; Angiono,Georgina; Lombardo,Daniel; Miceli,Dora; Argañaraz,Martin. |
| Oviductal molecules have the potential to improve the reproductive biotechnologies. In camelids, knowledge and assessment of the oviductal environment are necessary to successfully develop species-specific reproductive technologies, especially because of the camelids reproductive particularities. Among the oviductal factors, the matrix metalloproteinases/tissue inhibitor of matrix metalloproteinases system (MMPs/TIMPs) should be investigated more thoroughly due to their participation in reproductive processes. Consequently, the current study assayed gene and protein expression of MMPs throughout the llama oviduct. MMPs zymogen and active forms in the oviductal fluid were also characterized. MMP2 and MMP9 transcripts were detected in ampulla, isthmus,... |
| Tipo: Journal article |
Palavras-chave: Oviduct; Llama; MMPs; TIMPs; Gene expression levels. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200038 |
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| GALIZA, Y. S. de; ELOY, A. M. X.; PINHEIRO, R. R.; PEIXOTO, R. M.; LIMA, A. M. C.; BARROSO, M. L. da S.; FONSECA, L. M.. |
| Abstract: Caprine arthritis encephalitis is a lentiviral disease that leads to considerable losses in goat farming. In the acute phase of viral infection, though antiviral antibodies are produced by the host?s immune system, they are not sufficient to be detected by serological tests. Acute infections begin with an incubation period, during which the viral genome replicates and host innate responses are initiated. Matrix metalloproteinases (MMPs) are enzymes that play an important role in the physiological and pathological processes of tissue remodeling. The present study aimed to evaluate the expression of MMPs and their activity in the blood serum of male goats experimentally infected with caprine arthritis encephalitis virus (CAEV). Five dairy male... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: MMPs; Zimografia; Encefalite artrite caprina; Infecção experimental; CAEV; Zymography; Caprine arthritis encephalit virus; Experimental infection; Caprino; Doença Animal; Protease; Diagnostico; Virose Animal; Small ruminants; Goats; Goat diseases; Infectious diseases; Lentivirus; Caprine arthritis-encephalitis; Caprine arthritis encephalitis virus; Disease diagnosis; Semiarid zones; Brazil. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127176 |
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