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ACTIVIDAD ANTIPROLIFERATIVA DE ACEITES ESENCIALES DE PLANTAS CULTIVADAS EN COLOMBIA Acta biol.Colomb.
VELANDIA,Sindi Alejandra; QUINTERO,Elizabeth; STASHENKO,Elena E; OCAZIONEZ,Raquel E.
RESUMEN Colombia posee gran diversidad de plantas medicinales, pero pocas han sido objeto de investigación. En este trabajo se evaluó la actividad antiproliferativa de aceites esenciales obtenidos por hidrodestilación asistida por microondas. Se analizaron 15 muestras de 11 especies en ensayos del MTT en células cancerosas MCF-7, HeLa y HepG-2 y se incluyeron células normales humanas (HEK293) y de animales (Vero y B16F10) para evaluar selectividad. La composición química de muestras activas se determinó por cromatografía de gases acoplada a espectrometría de masas (GC-MS). Aceites esenciales de cuatro especies mostraron actividad antiproliferativa (CI50: 50 μg/mL) en células HeLa y/o MCF-7, en el siguiente rango (índice de selectividad en paréntesis):...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aceite esencial; Apoptosis; Cáncer; Fitoterapia; MTT.
Ano: 2018 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2018000200189
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Avaliação de citotoxicidade de nanofibras. Repositório Alice
SANTOS, M. F. da S.; RIZZI, M. S.; FEITOSA, L. O.; TEIXEIRA, E. M.; CORRÊA, A. C.; MATTOSO, L. H. C.; FRACETO, L. F.; LIMA, R. de.
bitstream/item/186165/1/Avaliacao-de-citotoxicidade-de-nanofibras..pdf
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Evento; Nanofibras; MTT; Citotoxidade; Algodão marrom; Algodão branco; Algodão rubi; Algodão verde.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/934614
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Bioisosteric modification on melatonin: synthesis of new naphthalene derivatives, in vitro antioxidant activity and cytotoxicity studies BJPS
Shirinzadeh,Hanif; Ghalia,Mohammed; Tascioglu,Alev; Adjali,Ferial Intissar; Gunesacar,Gulsen; Gurer-Orhan,Hande; Suzen,Sibel.
Melatonin (MLT) is a strong free radical scavenger that protects the body from the deleterious effects of excess oxidants. Synthesis of MLT analogue compounds with antioxidant potency has recently attracted the interest of researchers. In general, the strategy consists of modifying the groups in the different sites of the indole ring or replacing the indole ring with an analogue. As part of our ongoing research, the antioxidant capacity and cytotoxicity of newly synthesized MLT analogue naphthalene derivatives were evaluated. The radical scavenging activity was tested by a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Most of the synthesized compounds showed significant antioxidant activity in comparison to MLT. The structure-activity relationship was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Cytotoxic; Melatonin; MTT; DPPH.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100550
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Citotoxicidad de extracto acuoso de corteza de Manguifera indica L. (Vimang®) en Escherichia coli y linfocitos humanos Plantas Medicinales
Almeida Varela,Eliseo; Rosario Fernández,Luis Alfredo; Rodeiro Guerra,Idania; Cuétara Lugo,Elizabeth; Alonso Martín,Alena.
INTRODUCCIÓN: Vimang® es un producto de origen natural que se obtiene del árbol del mango (Manguifera indica L. familia Anacardiaceae). Este compuesto ha sido clasificado como antioxidante, inmunomodulador, etc. Por ello, resulta importante conocer su potencial citotóxico. OBJETIVOS: evaluar la citotoxicidad de un extracto acuoso del Vimang®. MÉTODOS: se emplearon los modelos procariótico (Escherichia coli, cepa PQ37) y eucariótico (eritrocitos humanos), se realizaron curvas de supervivencia celular con la cepa PQ37 (con activación metabólica y sin esta); así como se cuantificaron los niveles de la actividad fosfatasa alcalina (mediante la realización del SOS Chromotest). Posteriormente, se desarrolló el ensayo de inhibición de la actividad mitocondrial en...
Tipo: Journal article Palavras-chave: Vimang; Citotoxicidad; SOS Chromotest; PQ37; MTT; Eritrocitos humanos.
Ano: 2010 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962010000400005
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Cytotoxic evaluation of essential oil from Casearia sylvestris Sw on human cancer cells and erythrocytes Acta Amazonica
Silva,Saulo Luís da; Chaar,Jamal da Silva; Figueiredo,Patrícia de Maria Silva; Yano,Tomomasa.
A Casearia sylvestris (Flacourtiaceae) é uma planta popularmente conhecida como "guaçatonga" e é usada por povos indígenas da América do sul (Brasil, Peru e Bolivia) no tratamento de muitas doenças, incluindo câncer. Estudos citotóxicos mostraram que esta planta apresenta um possível e interessante potencial antitumoral devido à presença de moléculas chamadas casearinas. Além disso, a composição do óleo essencial mostrou uma alta concentração de sesquiterpenos de alto potencial citotóxico. Neste trabalho, nós verificamos que o óleo essencial da C. sylvestris apresentou uma boa citotoxicidade seletiva contra as linhagens de células tumorais HeLa, A-549 and HT-29 (CD50 63,3, 60,7 e 90,6 µg.ml-1, respectivamente) quando comparada às células não-tumorais Vero...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citotoxicidade; Casearia sylvestris; Eritrócitos; Óleo essencial; MTT; Sesquiterpenos.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000100012
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Effects of plant lectins on in vitro fibroblast proliferation BABT
Sell,Ana Maria; Costa,Celso Paulino da.
Lectins are carbohydrate-binding proteins that have been isolated from various sources and presented a wide spectrum of biological activities. The effects of four lectins, namely, Phaseolus vulgaris phytohemagglutinin, PHA, wheat germ agglutinin, WGA, Artocarpus integrifolia seed lectins, jacalin and artocarpin, on in vitro fibroblasts proliferation were investigated. The lectins did not influence the initial cell adhesion to the plate. PHA and WGA at 10-20 µg/mL concentrations significantly decreased fibroblasts proliferation. At these concentrations, they caused morphological alterations on cells and over 80 µg/mL, promoted cell death. Neither jacalin nor artocarpin significantly affected cell proliferation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lectins; Fibroblasts; Proliferation; MTT.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300006
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Human mesenchymal stem cells are resistant to cytotoxic and genotoxic effects of cisplatin in vitro Genet. Mol. Biol.
Bellagamba,Bruno Corrêa; Abreu,Bianca Regina Ribas de; Grivicich,Ivana; Markarian,Carolina Franke; Chem,Eduardo; Camassola,Melissa; Nardi,Nance Beyer; Dihl,Rafael Rodrigues.
Abstract Mesenchymal stem cells (MSCs) are known for their important properties involving multilineage differentiation potential., trophic factor secretion and localization along various organs and tissues. On the dark side, MSCs play a distinguished role in tumor microenvironments by differentiating into tumor-associated fibroblasts or supporting tumor growth via distinct mechanisms. Cisplatin (CIS) is a drug widely applied in the treatment of a large number of cancers and is known for its cytotoxic and genotoxic effects, both in vitro and in vivo. Here we assessed the effects of CIS on MSCs and the ovarian cancer cell line OVCAR-3, by MTT and comet assays. Our results demonstrated the resistance of MSCs to cell death and DNA damage induction by CIS,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Mesenchymal stem cells; Cisplatin; MTT; Comet assay; OVCAR-3 cells.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100129
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In silico, in vitro and in vivo toxicological assessment of BPP-BrachyNH2, A vasoactive proline-rich oligopeptide from Brachycephalus ephippium. Repositório Alice
ARCANJO, D. D. R.; MAFUD, A. C.; VASCONCELOS, A. G.; SILVA-FILHO, J. C. da; AMARAL, M. P. M.; BRITO, L. M.; BEMQUERER, M. P.; KÜCKELHAUS, S. A. S.; PLÁCIDO, A. P.; DELERUE-MATOS, C.; VALE, N.; MASCARENHAS, Y. P.; CARVALHO, F. A. A.; OLIVEIRA, A. P.; LEITE, J. R. S. A..
bitstream/item/181037/1/Arcanjo2017-Article-InSilicoInVitroAndInVivoToxico.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: MTT; In silico; PkCSM; Proline-rich oligopeptide; Toxicological; Cytotoxicity.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081643
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Investigations of Acacia modesta Wall. leaves for in vitro anti-diabetic, proliferative and cytotoxic effects BJPS
Saleem,Bushra; Islam,Muhammad; Saeed,Hamid; Imtiaz,Fariha; Asghar,Maryam; Saleem,Zikria; Mehmood,Azra; Naheed,Surriya.
ABSTRACT The leaves of Acacia modesta Wall. have been shown to possess diverse pharmacological properties. Therefore, we aimed at evaluating anti-diabetic, cytotoxic and proliferative effects of extracts of Acacia modesta Wall. leaves. After evaluating the primary and secondary metabolites, anti-diabetic activity of various extracts was assessed by α-amylase inhibition, glucose uptake by yeast cells and non-enzymatic glycosylation of hemoglobin assay. Cytotoxicity and proliferative potential was assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and short term proliferation assays, respectively, using human liver carcinoma cell line, HepG2. Among other extracts, chloroform extract exhibited 34.16% inhibition of α-amylase, 90.65%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acacia modesta Wall; /anti-diabetic effects/cytotoxic/in vitro; MTT; Phytochemical.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200609
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Polyphenols From Cutch Tree (Acacia catechu Willd.): Normalize In Vitro Oxidative Stress and Exerts Antiproliferative Activity BABT
Kumar,Rakesh; Arora,Rohit; Mahajan,Jyoti; Mahey,Sonia; Arora,Saroj.
ABSTRACT Oxidative stress, being the main cause of most of the human diseases, has always been the highlight of research worldwide. This stress can be overcome by administration of natural polyphenols. The Acacia catechu Willd. has many refrences available in Ayurveda as important disease curative plant. Its leaves are investigated for ameliorating oxidative stress in present work. Leaves of A. catechu were extracted with 80% methanol to get methanol extract (AME). It was assessed for antioxidant activity using DPPH, ABTS, CUPRAC, ferric ion reducing, superoxide scavenging and peroxyl radical scavenging assays. DNA protective activity was also investigated using plasmid nicking assay. Further, antiproliferative activity was determined using MTT assay in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acacia catechu; Antioxidant; Antiproliferative; UHPLC; MTT.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100316
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Rhein induces apoptosis of human gastric cancer SGC-7901 cells via an intrinsic mitochondrial pathway BJMBR
Li,Yiwen; Xu,Yuqing; Lei,Bo; Wang,Wenxiu; Ge,Xin; Li,Jingrui.
Rhein is a primary anthraquinone found in the roots of a traditional Chinese herb, rhubarb, and has been shown to have some anticancer effects. The aim of the present study was to investigate the effect of rhein on the apoptosis of the human gastric cancer line SGC-7901 and to identify the mechanism involved. SGC-7901 cells were cultured and treated with rhein (0, 50, 100, 150, and 200 µM) for 24, 48, or 72 h. Relative cell viability assessed by the MTT assay after treatment was 100, 99, 85, 79, 63% for 24 h; 100, 98, 80, 51, 37% for 48 h, and 100, 97, 60, 36, 15% for 72 h, respectively. Cell apoptosis was detected with TUNEL staining and quantified with flow cytometry using annexin FITC-PI staining at 48 h after 100, 200 and 300 µm rhein. The percentage...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhein; SGC-7901 cells; MTT; TUNEL; Flow cytometry; Apoptosis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100009
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Viability in protoplasts and cell suspensions of Coffea arabica cv. Catimor Electron. J. Biotechnol.
Fernandez-Da Silva,Rafael; Menéndez-Yuffá,Andrea.
Two methods were evaluated in order to assess the viability of protoplasts and cell suspensions of Coffea arabica cv. Catimor used in a protocol of transformation by electroporation. One method consisted of staining with 1 % Evans blue and the other staining with 1% 3-[4,5-dimethyltiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT). When Evans blue was applied to viable cells and protoplasts they either did not stain or acquired a faint blue colour. However, it was difficult to distinguish the non-viable cells in the wide spectrum of clear blue tonalities. In contrast, with the MTT assay only the viable cells and protoplasts reduced this salt to the red coloured formazan; viable and non-viable cells were distinguished more clearly with MTT than with Evans...
Tipo: Journal article Palavras-chave: Cell suspensions; Cell viability; Coffee; Evans blue; MTT; Protoplasts.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500016
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