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| Registros recuperados: 20 | |
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| Blecha,Isabella Maiumi Zaidan; Sousa,Isadora Inácio; Ferreira,Anna Beatriz Robottom; Feijó,Gelson Luis Dias; Torres Júnior,Roberto Augusto de Almeida; Siqueira,Fabiane. |
| ABSTRACT The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Animal breeding; Marker-assisted selection; PCR-RFLP; SNP; Tenderness. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100311 |
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| Fritsche-Neto,Roberto; Souza,Thiago Lívio Pessoa Oliveira de; Pereira,Helton Santos; Faria,Luís Cláudio de; Melo,Leonardo Cunha; Novaes,Evandro; Brum,Itaraju Junior Baracuhy; Jannink,Jean-Luc. |
| ABSTRACT: Despite important biotic stresses to common bean, Anthracnose (ANT) and Angular Leaf Spot (ALS) can cause losses of up to 80 % and occur in more than 60 countries around the world. Genetic resistance is the most sustainable strategy to manage these diseases. Thus, we aimed to (1) identify new SNP markers associated with ALS and ANT resistance loci in elite common bean lines, and (2) provide a functional characterization of the DNA sequences containing the identified SNP markers. We evaluated 60 inbred lines, under field conditions, which represent the elite germplasm developed by the Embrapa common bean breeding program across 22 years, in terms of severity of the ALS and ANT. The lines were genotyped with 5,398 SNPs. Then, a Mixed Linear Model... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colletotrichum lindemuthianum; Pseudocercospora griseola; Disease resistance; Elite lines; Marker-assisted selection. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001400321 |
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| Carvalho,Thiago Dutra de; Siqueira,Fabiane; Torres Júnior,Roberto Augusto de Almeida; Medeiros,Sérgio Raposo de; Feijó,Gelson Luís Dias; Souza Junior,Maury Dorta de; Blecha,Isabella Maiumi Zaidan; Soares,Cleber Oliveira. |
| The objective of the present study was to estimate the allelic and genotypic frequencies of the polymorphisms E2FB (AY138588.1: c.305C> T), located in the leptin gene (LEP), and TG5 (X05380.1:g.-422C>T), located in the thyroglobulin gene (TG), and evaluate the association of these polymorphisms in crossbred cattle of seven distinct genetic groups with the following traits: slaughter weight (SW), hot carcass weight (HCW), hot carcass yield (HCY), carcass fat thickness (CFT), ribeye area (REA), marbling (MARM) and shear force (SF). The animals were genotyped using the PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) technique, using 201 products obtained from F1 Caracu × Nellore, Angus × Nellore and Valdostana × Nellore... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fat thickness; Marker-assisted selection; Meat tenderness; Shear force; SNP. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000004 |
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| Nascimento,Carlos Souza do; Machado,Marco Antonio; Martinez,Mario Luiz; Silva,Marcos Vinícius G. Barbosa da; Guimarães,Marta Fonseca Martins; Campos,Ana Lúcia; Azevedo,Ana Luisa Sousa; Teodoro,Roberto Luiz; Verneque,Rui da Silva; Guimarães,Simone Eliza Facioni; Oliveira,Denise Aparecida Andrade. |
| The effect of the bovine major histocompatibility complex (BoLA) locus on animal health may be due to a direct action of its alleles on immune functions, whereas its indirect effect on production traits might be explained by the better general health conditions of more productive animals. In the present study, the BoLA-DRB3 gene was investigated in 1058 cows belonging to seven Brazilian Gyr Dairy herds (Bos indicus, Zebu cattle). A total of 37 alleles were identified, 15 of them described for the first time in a Zebu breed. A highly significant association (p < 0.02) was observed between allele *54 and a decrease (-26.1 kg) in milk protein yield and there was a significant association (p < 0.05) between this allele and lower (-26.07 kg) milk fat... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Marker-assisted selection; Molecular markers; PCR-RFLP. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400011 |
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| Mori, Masahiko; Onishi, Kazumitsu; Tokizono, Yoshiro; Shinada, Hiroshi; Yoshimura, Toru; Numao, Yoshinori; Miura, Hideho; Sato, Takashi; 大西, 一光; 三浦, 秀穂. |
| Cold stress at the booting stage in rice induces spikelet sterility because of aberrant microspore development, which often seriously damages seed production. Some breeding lines with high cold tolerance were developed by using tropical japonica variety Silewah as a donor of cold tolerance; however, the genetic factors that confer cold tolerance of this variety have not been comprehensively analyzed. In this study, phenotypic and molecular characterization of novel cold-tolerant strains derived from crosses with Silewah was performed to identify quantitative trait loci (QTLs) responsible for cold tolerance. Molecular marker analysis revealed that 2 cold-tolerant strains carried chromosomal segments of Silewah at the same genomic regions on chromosomes 3, 4... |
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Palavras-chave: Oryza sativa; Cold tolerance; Booting stage; QTL; Phenotypic selection; Marker-assisted selection; Exotic germplasm. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3040 |
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| Borchert,Thomas; Gawenda,Inka. |
| Calluna vulgaris is an important ornamental crop of the horticultural industry in Europe. In order to improve breeding of this species, especially of the most important trait of ‘bud-flowering', the implementation of molecular techniques that allow rapid, reproducible and efficient screening of whole segregating populations e.g. for molecular marker and mapping approaches is a requirement. We therefore aimed to introduce the powerful tool of amplified fragment length polymorphisms (AFLP®), a widely and successfully applied method, into our methodological assortment. As an essential prerequisite, the isolated DNA should be of adequate quality which is a common obstacle when dealing with woody species and their interfering secondary... |
| Tipo: Journal article |
Palavras-chave: DNA content; Mapping; Marker-assisted selection; Ornamental; Plant tissue. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200007 |
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| Duarte,Letícia Becker Homrich; Moraes,José Carlos Ferrugem; Weimer,Tania de Azevedo. |
| The genetic diversity of three microsatellites (ILSTS027, MBO22, BM4325) mapped on the bovine chromosome 15 and linked to the follicle-stimulating hormone beta gene (FSHß) was investigated in cows of a Brangus Ibagé herd and the efficiency of these markers for individual identification and parentage control was estimated. Possible associations between each molecular marker and cow reproductive performance were also analyzed. Six alleles were detected in BM4325 and ILSTS027 and 12 in MB022, the most frequent being BM4325*101, BM4325*103, ILSTS027*169 and MB022*229. Polymorphic information content ranged from 0.58 to 0.88 while expected heterozygosity ranged from 65% to 89%, with an average mean value of 77%. Although only three markers were studied, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellites; Bovine genetic diversity; Individual identification; Reproductive performance; Marker-assisted selection. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000100023 |
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| Cavalcanti,Lilian Cristina Gomes; Moraes,José Carlos Ferrugem; Faria,Danielle Assis de; McManus,Concepta Margaret; Nepomuceno,Alcebiades Renato; Souza,Carlos José Hoff de; Caetano,Alexandre Rodrigues; Paiva,Samuel Rezende. |
| Abstract: The objective of this work was to identify single nucleotide polymorphisms (SNPs) in resequencing data from MC1R, ASIP, and TYRP1 genes derived from Crioula sheep (Ovis aris) with different coat colors. Polymorphisms in the ASIP (agouti-signaling protein), MC1R (melanocortin 1 receptor), and TRYP1 (tyrosinase-related protein 1) genes were analyzed in 115 sheep from Embrapa’s conservation nucleus of crioula sheep, in Brazil. A total of 7,914 bp were sequenced per animal, and 14 SNPs were identified. Two additional assays were performed to detect duplications and deletions in the ASIP gene. Ninety-five percent of the coat color variation was explained by epistatic interactions observed between specific alleles in the MC1R and ASIP genes. Evidence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovis aris; ASIP; Genetic resources conservation; Marker-assisted selection; MC1R; TYRP1. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000800615 |
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| Seido, Sirando Lima; Fernandes Santos, Carlos Antonio. |
| The objectives of the present study were to construct a cowpea genetic map using the F2 population resulting from the cross IC-1 x BRS Marataoã, based on single nucleotide polymorphism (SNP) markers, and to map the cpi gene, with additional reference to introgression with the consensus map of species, aiming to identify markers for assisted selection to develop more efficient cultivars for BNF. The parents and 89 F2 plants were genotyped with 51,128 SNP markers, of which 910 polymorphic markers were used to construct the map. The results revealed 11 linkage groups, with an average of 82 markers per chromosome and average distance of 1.26 cM between markers. Recombination analysis of the SNPs indicated that markers 2_12850 and 2_00188, located in linkage... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vigna unguiculata; Nitrogen fixation; Marker-assisted selection. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42603 |
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| Leroy,Thierry; Ribeyre,Fabienne; Bertrand,Benóit; Charmetant,Pierre; Dufour,Magali; Montagnon,Christophe; Marraccini,Pierre; Pot,David. |
| Coffee quality, in the present context of overproduction worldwide, has to be considered as a main selection criterion for coffee improvement. After a definition of quality, and an overview of the non genetic factors affecting its variation, this review focuses on the genetic factors involved in the control of coffee quality variation. Regarding the complexity of this trait, the different types of quality are first presented. Then, the great variation within and between coffee species is underlined, mainly for biochemical compounds related to quality (caffeine, sugars, chlorogenic acids, lipids). The ways for breeding quality traits for cultivated species, Coffea arabica and Coffea canephora are discussed, with specific challenges for each species. For C.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea spp.; Biochemical compounds; Candidate genes; ESTs; Genetic breeding; Marker-assisted selection; Quality. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100016 |
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| Ferreira,Marcia Flores da Silva; Cervigni,Gerardo Domingo Lucio; Ferreira,Adésio; Schuster,Ivan; Santana,Fernanda Abreu; Pereira,Waldir Dias; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves. |
| The objective of this work was to identify major and minor-effect quantitative trait loci (QTL) for resistance to races 3, 9, and 14 of soybean cyst nematode (SCN) in Hartwig cultivar; to map new resistance QTLs for these races; and to check for the existence of epistatic interactions between QTLs. Cultivar Hartwig is an important resistance source to SCN. Recombinant inbred lines (RIL) obtained from a cross between 'Hartwig' (resistant) and Y23 (susceptible) were evaluated regarding resistance to the three races. New genomic regions for resistance to SCN were identified by microsatellites. Four QTLs, which explained between 12 and 34% of phenotypic variance, were detected for resistance to race 3 in linkage groups (LG) A2, G, J, and M. The QTL in LG G is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Breeding; Marker-assisted selection; Molecular markers. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400012 |
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| Registros recuperados: 20 | |
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