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A deterministic simulation study of embryo marker-assisted selection for age at first calving in Nellore (Bos indicus) beef cattle Genet. Mol. Biol.
Rosa,Artur J.M.; Bijma,Piter; Oliveira,Henrique N.; Lôbo,Raysildo B.; van Arendonk,Johan A.M..
We used deterministic simulation of four alternative multiple ovulation and embryo manipulation (MOET) closed nucleus schemes to investigate the benefits of using marker-assisted selection (MAS) of Nellore (Bos indicus) beef cattle embryos prior to transplantation to reduce the age at first calving (AFC). We found that MAS resulted in increased genetic gain as compared to selection without AFC quantitative trait loci (AFC-QTL) information. With single-stage selection the genetic response (GR) increased as follows: GR = 0.68% when the AFC-QTL explained 0.02 of the AFC additive genetic variance (sigma2A); GR = 1.76% for AFC-QTL explaining 0.05 sigma2A; GR = 3.7% for AFC-QTL explaining 0.1 sigma2A; and GR = 55.76% for AFC-QTL explaining 0.95 sigma2A. At the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Age at first calving; Beef cattle; Marker-assisted selection; Pre-selection; Quantitative trait loci.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400008
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A mixed model to multiple harvest-location trials applied to genomic prediction in Coffea canephora. Repositório Alice
FERRÃO, L. F. V.; FERRÃO, R. G.; FERRAO, M. A. G.; FONSECA, A. F. A. da; GARCIA, A. A. F..
Genomic selection (GS) has been studied in several crops to increase the rates of genetic gain and reduce the length of breeding cycles. Despite its relevance, there are only a modest number of reports applied to the genus Coffea. Effective implementation depends on the ability to consider genomic models, which correctly represent breeding scenario in which the species are inserted. Coffee experimentation, in general, is represented by evaluations in multiple locations and harvests to understand the interaction and predict the performance of untested genotypes. Therefore, the main objective of this study was to investigate GS models suitable for use in Coffea canephora. An expansion of traditional GBLUP was considered and genomic analysis was performed...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Genotyping-by-sequencing; GBLUP; Multi-environment trials; Perennial crops; Marker-assisted selection.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081803
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Accuracy of genomic prediction for tick resistance in Braford and Hereford cattle. Repositório Alice
CARDOSO, F. F.; SOLLERO, B. P.; GOMES, C. C. G.; COMIN, H. B.; ROSO, V. M.; HIGA, R. H.; YOKOO, M. J.; CAETANO, A. R.; AGUILAR, I..
This work aimed to determine the accuracy and bias of genomic predictions of Braford (BO) and Hereford (HH) cattle genetic resistance to ticks.
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Seleção genômica; Resistência a carrapatos; Tick resistance; Gado de corte; Beef cattle; Marker-assisted selection.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1003697
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Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle. Repositório Alice
BLECHA, I. M. Z.; SOUSA, I. I.; FERREIRA, A. B. R.; FEIJO, G. L. D.; TORRES JUNIOR, R. A. de A.; SIQUEIRA, F..
The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Animal breeding; Marker-assisted selection; Meat tenderness.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117678
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Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle R. Bras. Zootec.
Blecha,Isabella Maiumi Zaidan; Sousa,Isadora Inácio; Ferreira,Anna Beatriz Robottom; Feijó,Gelson Luis Dias; Torres Júnior,Roberto Augusto de Almeida; Siqueira,Fabiane.
ABSTRACT The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal breeding; Marker-assisted selection; PCR-RFLP; SNP; Tenderness.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100311
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Assisted-selection of naturally caffeine-free coffee cultivars?characterization of SNPs from a methyltransferase gene. Repositório Alice
FAVORETTO, P.; SILVA, C. C. da; TAVARES, A. G.; GIATTI, G.; MORAES, P. F.; LOBATO, M. T. V.; SILVAROLLA, M. B.; OLIVEIRO FILHO, G.; MALUF, M. P..
Breeding of caffeine-free coffee cultivars require tools for an early selection of progenies bearing this later trait. Genes from caffeine synthesis and degradation represent major targets for the development of molecular markers for assisted selection. In this study, we characterized SNPs identified on the caffeine synthase gene from AC1 mutant, a naturally caffeine-free arabica coffee plant. Molecular analysis of normal and mutant sequences indicates the occurrence of SNPs in protein domains, potentially associated with caffeine synthesis in coffee. Progenies F2, F1BC1 and BC from crosses of AC mutants and elite cultivars were evaluated regarding caffeine content in grains and genomic segregation profile of selected SNPs. Genotyping analysis allowed the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Caffeine content; Caffeine synthase; Coffee; Molecular markers; Marker-assisted selection.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113244
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Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance. Repositório Alice
FRITSCHE-NETO, R.; SOUZA, T. L. P. O. de; PEREIRA, H. S.; FARIA, L. C. de; MELO, L. C.; NOVAES, E.; BRUM, I. J. B.; JANNINK, J-L..
Despite important biotic stresses to common bean, Anthracnose (ANT) and Angular Leaf Spot (ALS) can cause losses of up to 80 % and occur in more than 60 countries around the world. Genetic resistance is the most sustainable strategy to manage these diseases. Thus, we aimed to (1) identify new SNP markers associated with ALS and ANT resistance loci in elite common bean lines, and (2) provide a functional characterization of the DNA sequences containing the identified SNP markers. We evaluated 60 inbred lines, under field conditions, which represent the elite germplasm developed by the Embrapa common bean breeding program across 22 years, in terms of severity of the ALS and ANT. The lines were genotyped with 5,398 SNPs. Then, a Mixed Linear Model was run to...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Feijão; Phaseolus Vulgaris; Antracnose; Resistência; Mancha Angular; Anthracnose; Beans; Colletotrichum lindemuthianum virus; Pseudocercospora; Disease resistance; Marker-assisted selection.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1111232
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Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance Scientia Agricola
Fritsche-Neto,Roberto; Souza,Thiago Lívio Pessoa Oliveira de; Pereira,Helton Santos; Faria,Luís Cláudio de; Melo,Leonardo Cunha; Novaes,Evandro; Brum,Itaraju Junior Baracuhy; Jannink,Jean-Luc.
ABSTRACT: Despite important biotic stresses to common bean, Anthracnose (ANT) and Angular Leaf Spot (ALS) can cause losses of up to 80 % and occur in more than 60 countries around the world. Genetic resistance is the most sustainable strategy to manage these diseases. Thus, we aimed to (1) identify new SNP markers associated with ALS and ANT resistance loci in elite common bean lines, and (2) provide a functional characterization of the DNA sequences containing the identified SNP markers. We evaluated 60 inbred lines, under field conditions, which represent the elite germplasm developed by the Embrapa common bean breeding program across 22 years, in terms of severity of the ALS and ANT. The lines were genotyped with 5,398 SNPs. Then, a Mixed Linear Model...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Colletotrichum lindemuthianum; Pseudocercospora griseola; Disease resistance; Elite lines; Marker-assisted selection.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001400321
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Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle R. Bras. Zootec.
Carvalho,Thiago Dutra de; Siqueira,Fabiane; Torres Júnior,Roberto Augusto de Almeida; Medeiros,Sérgio Raposo de; Feijó,Gelson Luís Dias; Souza Junior,Maury Dorta de; Blecha,Isabella Maiumi Zaidan; Soares,Cleber Oliveira.
The objective of the present study was to estimate the allelic and genotypic frequencies of the polymorphisms E2FB (AY138588.1: c.305C> T), located in the leptin gene (LEP), and TG5 (X05380.1:g.-422C>T), located in the thyroglobulin gene (TG), and evaluate the association of these polymorphisms in crossbred cattle of seven distinct genetic groups with the following traits: slaughter weight (SW), hot carcass weight (HCW), hot carcass yield (HCY), carcass fat thickness (CFT), ribeye area (REA), marbling (MARM) and shear force (SF). The animals were genotyped using the PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) technique, using 201 products obtained from F1 Caracu × Nellore, Angus × Nellore and Valdostana × Nellore...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fat thickness; Marker-assisted selection; Meat tenderness; Shear force; SNP.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000004
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Association of the bovine major histocompatibility complex (BoLA) BoLA-DRB3 gene with fat and protein production and somatic cell score in Brazilian Gyr dairy cattle (Bos indicus) Genet. Mol. Biol.
Nascimento,Carlos Souza do; Machado,Marco Antonio; Martinez,Mario Luiz; Silva,Marcos Vinícius G. Barbosa da; Guimarães,Marta Fonseca Martins; Campos,Ana Lúcia; Azevedo,Ana Luisa Sousa; Teodoro,Roberto Luiz; Verneque,Rui da Silva; Guimarães,Simone Eliza Facioni; Oliveira,Denise Aparecida Andrade.
The effect of the bovine major histocompatibility complex (BoLA) locus on animal health may be due to a direct action of its alleles on immune functions, whereas its indirect effect on production traits might be explained by the better general health conditions of more productive animals. In the present study, the BoLA-DRB3 gene was investigated in 1058 cows belonging to seven Brazilian Gyr Dairy herds (Bos indicus, Zebu cattle). A total of 37 alleles were identified, 15 of them described for the first time in a Zebu breed. A highly significant association (p < 0.02) was observed between allele *54 and a decrease (-26.1 kg) in milk protein yield and there was a significant association (p < 0.05) between this allele and lower (-26.07 kg) milk fat...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Marker-assisted selection; Molecular markers; PCR-RFLP.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400011
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Desenvolvimento de marcadores associados à resistência do bicho-mineiro: seleção e validação de SNPs. Repositório Alice
BAZIOLI, J. M.; GUIMARÃES, P. de S.; STRAZZA, B. A.; SCHENK, J. C. M.; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P..
O objetivo deste estudo é o desenvolvimento de marcadores do tipo SNPs associados com a resistência ao bicho-mineiro em café. A estratégia principal utilizou as análises genômicas de microarranjo, para caracterização de expressão diferencial de genes-candidatos em plantas resistentes infestadas com o bicho-mineiro. Inicialmente, análises in silico de 2137 destes genes identificaram aqueles potencialmente relacionados com a especificidade da resposta de defesa da planta. Uma vez selecionados, o perfil de expressão de 22 genes foi confirmado por qRT-PCR em experimentos utilizando plantas resistentes e suscetíveis, infectadas pelo bicho-mineiro. Para busca de polimorfismos do tipo SNPs, que poderão ser utilizados como marcadores para seleção-assistida,...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Seleção-assistida; Estresse biótico; Melhoramento molecular; Molecular breeding; Marker-assisted selection; Biotic stress.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113382
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Detection of a novel quantitative trait locus for cold tolerance at the booting stage derived from a tropical japonica rice variety Silewah OAK
Mori, Masahiko; Onishi, Kazumitsu; Tokizono, Yoshiro; Shinada, Hiroshi; Yoshimura, Toru; Numao, Yoshinori; Miura, Hideho; Sato, Takashi; 大西, 一光; 三浦, 秀穂.
Cold stress at the booting stage in rice induces spikelet sterility because of aberrant microspore development, which often seriously damages seed production. Some breeding lines with high cold tolerance were developed by using tropical japonica variety Silewah as a donor of cold tolerance; however, the genetic factors that confer cold tolerance of this variety have not been comprehensively analyzed. In this study, phenotypic and molecular characterization of novel cold-tolerant strains derived from crosses with Silewah was performed to identify quantitative trait loci (QTLs) responsible for cold tolerance. Molecular marker analysis revealed that 2 cold-tolerant strains carried chromosomal segments of Silewah at the same genomic regions on chromosomes 3, 4...
Palavras-chave: Oryza sativa; Cold tolerance; Booting stage; QTL; Phenotypic selection; Marker-assisted selection; Exotic germplasm.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3040
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Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae) Electron. J. Biotechnol.
Borchert,Thomas; Gawenda,Inka.
Calluna vulgaris is an important ornamental crop of the horticultural industry in Europe. In order to improve breeding of this species, especially of the most important trait of &#8216;bud-flowering', the implementation of molecular techniques that allow rapid, reproducible and efficient screening of whole segregating populations e.g. for molecular marker and mapping approaches is a requirement. We therefore aimed to introduce the powerful tool of amplified fragment length polymorphisms (AFLP®), a widely and successfully applied method, into our methodological assortment. As an essential prerequisite, the isolated DNA should be of adequate quality which is a common obstacle when dealing with woody species and their interfering secondary...
Tipo: Journal article Palavras-chave: DNA content; Mapping; Marker-assisted selection; Ornamental; Plant tissue.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200007
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Diversity of microsatellites linked to the FSHbeta gene, their usefulness for individual identification and association with reproductive performance Ciência Rural
Duarte,Letícia Becker Homrich; Moraes,José Carlos Ferrugem; Weimer,Tania de Azevedo.
The genetic diversity of three microsatellites (ILSTS027, MBO22, BM4325) mapped on the bovine chromosome 15 and linked to the follicle-stimulating hormone beta gene (FSHß) was investigated in cows of a Brangus Ibagé herd and the efficiency of these markers for individual identification and parentage control was estimated. Possible associations between each molecular marker and cow reproductive performance were also analyzed. Six alleles were detected in BM4325 and ILSTS027 and 12 in MB022, the most frequent being BM4325*101, BM4325*103, ILSTS027*169 and MB022*229. Polymorphic information content ranged from 0.58 to 0.88 while expected heterozygosity ranged from 65% to 89%, with an average mean value of 77%. Although only three markers were studied, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Bovine genetic diversity; Individual identification; Reproductive performance; Marker-assisted selection.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000100023
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Efficiency in evaluating salt tolerance in rice using phenotypic and marker assisted selection International Rice Research Institute
Bhuiyan, Md. A.R..
xvi, 96 p. : ill. Thesis (Ph.D.) -- Bangladesh Agricultural University.
Tipo: Thesis Palavras-chave: Rice; Plant genetics; Salt tolerance; Selection methods; Marker-assisted selection; Plant breeding.
Ano: 2005 URL: http://hdl.handle.net/10269/179
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Efficiency of microsatellite markers in assisted selection for resistance to soybean cyst nematode (race 3). Repositório Alice
DI MAURO, A. O.; UNÊDA-TREVISOLI, S. H.; DI MAURO, S. M. Z.; COSTA, M. M.; OLIVEIRA, R. C. de; ARANTES, N. E..
Four microsatellite sequences were tested in soybean DNA from cultivars and segregating genotypes. Three of them were close to the resistance locus rhg 1 on molecular linkage group G (Satt 309, Sat_168, Sat_163) and one was close to Rhg4 locus on group A2 (Sat_162). Progenies previously classified as cyst nematode (SNC) resistant and others with unknown reaction were tested, using the resistant cultivars Liderança, and Renascença and the susceptibles 'Cristalina' and 'OCEPAR-4' as control. The best primer for resistance to SCN was Sat_162. Twenty segregant progenies tested with Sat_162 presented a 150 bp band for homozygous resistant genotypes and 200 bp for susceptible ones, and both for the heterozigous genotypes. Previous studies have shown that this...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Soja; Nematóde; Heterodera glycines; Soybeans; Nematoda; Heterodera glycines; Marker-assisted selection.
Ano: 2004 URL: http://www.alice.cnptia.embrapa.br/handle/doc/467572
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Estimation by simulation of the efficiency of the French marker assisted selection program in dairy cattle Inra
Guillaume, F.; Fritz; Boichard, D.; Druet, T..
Tipo: Journal Article Palavras-chave: Marker-assisted selection; Simulation; Efficiency; Dairy cattle.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008f2993140&uri=/notices/prodinra1/2009/12/
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Estudo de associação de polimorfismos nos genes LEP e TG com características de qualidade de carne e carcaça em bovinos da raça Canchim. Repositório Alice
SOUSA, I. I.; BLECHA, I. M. Z.; MACIEL, S. da S.; FERREIRA, A. B. R.; FEIJO, G. L. D.; FAVERO, R.; SANTIAGO, G. G.; SIQUEIRA, F..
Avaliou-se os marcadores moleculares do tipo SNP (Single-nucleotide Polymorphism) E2FB (AY138588.1:c.305C>T) e TG5 (X05380.1:g.-422C>T) nos genes da leptina e da tireoglobulina em animais da raça Canchim. As genotipagens foram realizadas por PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) e as frequências alélicas e genotípicas foram comparadas pelo teste Quiquadrado. Os desvios de frequências observadas em relação às esperadas para o Equilíbrio de Hardy-Weinberg foram analisados a 5% de significância e os dados foram submetidos à ANOVA por meio do PROC GLM (SAS). No marcador LEP/Kpn2I observou-se associação sugestiva (P=0,0555) para área de olho de lombo e marmoreio (P=0,068), porém não foi verificado efeito...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Seleção assistida por marcadores; Gado de corte; Marcador moleculr; Melhoramento genético animal; Beef cattle; Genetic markers; Genetic improvement; Marker-assisted selection.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1077308
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Genetic characterization of coat color genes in Brazilian Crioula sheep from a conservation nucleus PAB
Cavalcanti,Lilian Cristina Gomes; Moraes,José Carlos Ferrugem; Faria,Danielle Assis de; McManus,Concepta Margaret; Nepomuceno,Alcebiades Renato; Souza,Carlos José Hoff de; Caetano,Alexandre Rodrigues; Paiva,Samuel Rezende.
Abstract: The objective of this work was to identify single nucleotide polymorphisms (SNPs) in resequencing data from MC1R, ASIP, and TYRP1 genes derived from Crioula sheep (Ovis aris) with different coat colors. Polymorphisms in the ASIP (agouti-signaling protein), MC1R (melanocortin 1 receptor), and TRYP1 (tyrosinase-related protein 1) genes were analyzed in 115 sheep from Embrapa’s conservation nucleus of crioula sheep, in Brazil. A total of 7,914 bp were sequenced per animal, and 14 SNPs were identified. Two additional assays were performed to detect duplications and deletions in the ASIP gene. Ninety-five percent of the coat color variation was explained by epistatic interactions observed between specific alleles in the MC1R and ASIP genes. Evidence...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovis aris; ASIP; Genetic resources conservation; Marker-assisted selection; MC1R; TYRP1.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000800615
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Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpea Agronomy
Seido, Sirando Lima; Fernandes Santos, Carlos Antonio.
The objectives of the present study were to construct a cowpea genetic map using the F2 population resulting from the cross IC-1 x BRS Marataoã, based on single nucleotide polymorphism (SNP) markers, and to map the cpi gene, with additional reference to introgression with the consensus map of species, aiming to identify markers for assisted selection to develop more efficient cultivars for BNF. The parents and 89 F2 plants were genotyped with 51,128 SNP markers, of which 910 polymorphic markers were used to construct the map. The results revealed 11 linkage groups, with an average of 82 markers per chromosome and average distance of 1.26 cM between markers. Recombination analysis of the SNPs indicated that markers 2_12850 and 2_00188, located in linkage...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vigna unguiculata; Nitrogen fixation; Marker-assisted selection.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42603
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