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Excitatory effects of Buthus C56 toxin on Drosophila larval neuromuscular junction J. Venom. Anim. Toxins incl. Trop. Dis.
Gawade,S. P..
Buthus C56 toxin from venom of the Indian red scorpion Mesobuthus tamulus was studied for its effects on spontaneous miniature excitatory junctional potentials (MEJP) on Drosophila larval neuromuscular junctions. C56 toxin was isolated on CM-Cellulose with linear gradient of ammonium acetate buffer, pH 6.0. Toxin purity was determined on SDS slab gel electrophoresis. Effective concentration of C56 toxin was based on contraction paralysis units (CPU) in Drosophila 3rd instar larvae by microinjection (0.1 CPU/ml = 2 x 10-6 g/ml). The toxin-induced excitatory junctional potentials were studied for calcium dependency (0.2 mM to 1.2 mM Ca2+) in Drosophila Ringer. Excitatory junctional potential amplitude was increased with increasing calcium concentration;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesobuthus tamulus; C56 toxin; Drosophila melanogaster; Neuromuscular; Miniature excitatory junctional potentials (MEJPs).
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100004
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Group III PLA2 from the scorpion, Mesobuthus tamulus: cloning and recombinant expression in E. coli Electron. J. Biotechnol.
Hariprasad,Gururao; Saravanan,Kolandaivelu; Singh,Sundararajan Baskar; Das,Utpal; Sharma,Sujata; Kaur,Punit; Singh,Tej Pal; Srinivasan,Alagiri.
Phospholipases A2 (PLA2) are enzymes that specifically hydrolyze the sn-2 fatty acid acyl bond of phospholipids, producing a free fatty acid and a lyso-phospholipid. We report the cloning and expression of a secretory phospholipase A2 (sPLA2) from Mesobuthus tamulus, Indian red scorpion. The nucleotide sequence codes for a 167 residue enzyme. The open reading frame codes for a 31 amino acid signal peptide followed by a mature portion of the protein. The primary structure shows the calcium binding motif, catalytic residues, 8 highly-conserved cysteines and C-terminal extension which classify it as a group III PLA2. The entire transcript was expressed in Escherichia coli and was purified by metal affinity chromatography under denaturing conditions. The...
Tipo: Journal article Palavras-chave: Group III phospholipase A2; Mesobuthus tamulus; Recombinant expression.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300006
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Intraspecific variation in protein pattern of red scorpion (Mesobuthus tamulus, coconsis, pocock) venoms from Western and Southern India J. Venom. Anim. Toxins incl. Trop. Dis.
Badhe,R. V.; Thomas,A. B.; Harer,S. L.; Deshpande,A. D.; Salvi,N.; Waghmare,A..
Red scorpions Mesobuthus tamulus (Coconsis, Pocock) were obtained from different regions of West and South India (Ratnagiri, Chiplun and Ahmednagar from Maharashtra and Chennai from Tamil Nadu, respectively). Their venoms composition was analyzed using gel electrophoresis (SDS-PAGE). All venom samples shared six bands of 170, 80, 60, 57, 43, and 38 kDa molecular weights. Bands of 115 kDa and 51.5 kDa were characteristic of venoms obtained from red scorpions of Chiplun region, and the 26kDa band was absent in scorpion venom from Tamil Nadu. The separated protein band patterns suggest that the venoms from Ratnagiri, Ahmednagar and Tamil Nadu had high similarities in their biochemical composition but differed from that of Chiplun region. These data were also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Red scorpion; SDS-PAGE; Mesobuthus tamulus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400008
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