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An approach on phytoalexins: function, characterization and biosynthesis in plants of the family Poaceae Ciência Rural
Arruda,Rejanne Lima; Paz,Andressa Tuane Santana; Bara,Maria Teresa Freitas; Côrtes,Márcio Vinicius de Carvalho Barros; Filippi,Marta Cristina Corsi de; Conceição,Edemilson Cardoso da.
ABSTRACT: Phytoalexins are compounds that have been studied a few years ago, which present mainly antimicrobial activity. The plants of the family Poaceae are the most geographically widespread and stand out for their economic importance, once they are cereals used as staple food. This family stands out for having a variety of phytoalexins, which can be synthesized via the shikimic acid (the phenylpropanoids), or mevalonic acid, being considered terpenoid phytoalexins. The characterization of these compounds with antimicrobial activity is carried out using chromatographic techniques, and the high-performance liquid chromatography (HPLC) coupled with mass spectrometry are the most efficient methods in this process. This research aimed to present an approach...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metabolites; Protection of plants; Signaling; Oryza sativa.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701206
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Atrazine degradation patterns: the role of straw cover and herbicide application history Scientia Agricola
Leal,Daniela Piaz Barbosa; Dick,Deborah Pinheiro; Stahl,Anna Meike; Köppchen,Stephan; Burauel,Peter.
ABSTRACT: In Brazil, atrazine (ATZ) is widely applied to maize (Zea mays L.) fields for weed control. The presence of ATZ and its metabolites in soil and water matrices has become a matter of some concern for governmental authorities as well as for society at large. This study evaluated the patterns of ATZ degradation (mineralization, extractable and non-extractable ATZ residues, and metabolite formation) in a Brazilian Typic Paleudult. Soil samples from a cultivated area under a no-tillage system with a history of ATZ application were incubated with 14C-ATZ in both the presence and absence of straw cover on the soil surface, and the evolved 14CO2 was determined by liquid scintillation. Samples from an area with native vegetation, adjacent to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atrazine mineralization; Sorption; Metabolites; No-tillage.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019000100063
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Biodegradation of kerosene: Study of growth optimization and metabolic fate of P. janthinellum SDX7 BJM
Khan,Shamiyan R.; Nirmal,J.I. Kumar; Kumar,Rita N.; Patel,Jignasha G..
Penicillum janthinellum SDX7 was isolated from aged petroleum hydrocarbon-affected soil at the site of Anand, Gujarat, India, and was tested for different pH, temperature, agitation and concentrations for optimal growth of the isolate that was capable of degrading upto 95%, 63% and 58% of 1%, 3% and 5% kerosene, respectively, after a period of 16 days, at optimal growth conditions of pH 6.0, 30 °C and 180 rpm agitation. The GC/MS chromatograms revealed that then-alkane fractions are easily degraded; however, the rate might be lower for branched alkanes, n-alkylaromatics, cyclic alkanes and polynuclear aromatics. The test doses caused a concentration-dependent depletion of carbohydrates of P. janthinellum SDX7 by 3% to 80%, proteins by 4% to 81% and amino...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Penicillum janthinellum SDX7; Optimal growth; Metabolites; Kerosene degradation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200397
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Biosynthesis of D-Mannitol by Diplodia pinea. Repositório Alice
MOURA, P. F.; GRIBNER, C.; RECH, K. S.; GATTO, L. J.; RIGONI, A. A. R.; BETIM, F. C. M.; WGNER, R.; DIAS, J. de F. G.; MIGUEL, O. G.; AUER, C. G.; MIGUEL, M. D..
Resumo As pesquisas com fungos em busca de substâncias com potencial atividade biológica ganham cada vez mais destaque por serem fontes sustentáveis de novas moléculas. Diplodia pinea é um fungo fitopatogênico encontrado em espécies florestais, que tem apresentado poucos relatos de moléculas com atividade biológica e pode apresentar potencial biotecnológico promissor. Assim, o objetivo deste estudo foi detectar substâncias produzidas por esse fungo em meio líquido, sob condições de estresse. Um metabólito foi detectado na fração alcoólica e caracterizado por difração de raios-X como D-manitol. Esta é a primeira descrição deste monossacarídeo produzido por D. pinea. Abstract Research with fungi in search of substances with potential biological activity is...
Tipo: Artigo de periódico Palavras-chave: Difração de raios X; Metabólitos; Carboidrato; Biotecnologia; Fungo; Carbohydrates; Biotechnology; Fungi; Metabolites; X-ray diffraction.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1132796
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Early detection and classification of Carica papaya fungi trough the analysis of volatile metabolites by gas chromatography and chemometrics. Repositório Alice
TERRA, L. R.; QUEIROZ, S. C. do N. de; TERAO, D.; FERREIRA, M. M. C..
Papaya (Carica papaya) is a fruit widely consumed in the world due to its pleasant taste and high nutritional value. Brazil stands out as one of the world?s largest producers and exporters of papaya. Concomitant to the rapid expansion of fruit production and increase in export, the phytosanitary problems have grown, such as diseases caused by post-harvest fungi, which results in enormous losses to those involved in the export1. Conventional methodologies for detection and classification of fungi are time-consuming, laborious and can only be performed after the manifestation of symptoms in fruits2. Thus, it is necessary to develop methods that allow the early diagnosis of the presence of fungi, which are fast, efficient and that permit the qualitative...
Tipo: Separatas Palavras-chave: Carica Papaya; Cromatografia; Metabolites.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116541
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Effect of exercise on occurrence of diurnal rhythms of plasma ions and metabolites in Thoroughbred racehorses Arq. Bras. Med. Vet. Zootec.
Viana,P.C.R.; Caldas-Bussiere,M.C.; Marins,R.S.Q.S.; Menna-Barreto,L.S.; Cury,L.J..
Records of plasma calcium (Ca++), phosphorus (Pi), potassium (K+), sodium (Na+), chloride (Cl-), magnesium (Mg++), iron (Fe++), glucose, cholesterol, triglycerides and total protein levels were measured to determine the effects of exercise on occurrence of diurnal rhythms in Throughbred racehorses (n=7) under physical training. Physical activities consisted of gallop on the track and walking. Blood samples were collected from jugular vein every 4h over a 48h period. Plasma Ca++, K+, Mg++ and Na+ levels were obtained by flame photometry; and, Pi, Cl-, Fe++, glucose, cholesterol, triglycerides and total protein levels were measured by colorimetric tests using visible UV spectrophotometry. The data were analyzed using a 24h period to each exercise performed....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Horse; Chronobiology; Exercise; Ions; Metabolites; Diurnal rhythms.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000400006
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Effect of hypoxia on the activity and binding of glycolytic and associated enzymes in sea scorpion tissues BJMBR
Lushchak,V.I.; Bahnjukova,T.V.; Storey,K.B..
The effect of hypoxia on the levels of glycogen, glucose and lactate as well as the activities and binding of glycolytic and associated enzymes to subcellular structures was studied in brain, liver and white muscle of the teleost fish, Scorpaena porcus. Hypoxia exposure decreased glucose levels in liver from 2.53 to 1.70 µmol/g wet weight and in muscle led to its increase from 3.64 to 25.1 µmol/g wet weight. Maximal activities of several enzymes in brain were increased by hypoxia: hexokinase by 23%, phosphoglucoisomerase by 47% and phosphofructokinase (PFK) by 56%. However, activities of other enzymes in brain as well as enzymes in liver and white muscle were largely unchanged or decreased during experimental hypoxia. Glycolytic enzymes in all three...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Sea scorpion; Scorpaena porcus; Hypoxia; Metabolites; Glycolytic enzyme binding.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800005
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Effect of pulsed light on postharvest disease control-related metabolomic variation in melon (Cucumis melo) artificially inoculated with Fusarium pallidoroseum. Repositório Alice
OIRAM FILHO, F.; SILVA, E. de O.; LOPES, M. M. de A.; RIBEIRO, P. R. V.; OSTER, A. H.; GUEDES, J. A. C.; ZAMPIERI, D. de S.; BORDALLO, P. do N.; ZOCOLO, G. J..
Tipo: Artigo de periódico Palavras-chave: Metabolitos; Biomarcadores; Patógenos fúngicos; Doenças fúngicas; Pulsos de luz; Resistência das plantas ao estresse abiótico; Fungal pathogens; Plant resistance to abiotic stress; Light pulses; Fungal diseases; Melão; Melons; Fruits; Biomarkers; Metabolites.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123192
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Extraction and Simultaneous Determination of Glyphosate, AMPA and Compounds of the Shikimic Acid Pathway in Plants Planta Daninha
Gomes,G.L.G.C.; Carbonari,C.A.; Velini,E.D.; Trindade,M.L.B.; Silva,J.R.M..
This study has aimed to develop a method for simultaneous extraction and determination by liquid chromatography and mass spectrometry (LC-MS/MS) of glyphosate, aminomethylphosphonic acid (AMPA), shikimic acid, quinic acid, phenylalanine, tyrosine and tryptophan. For the joint analysis of these compounds the best conditions of ionization in mass spectrometry and for chromatographic separation of the compounds were selected. Calibration curves and linearity ranges were also determined for each compound. Different extraction systems of the compounds were tested from plant tissues collected from sugarcane (Saccharum officinarum) and eucalyptus (Eucalyptus urophylla platiphylla) plants two days after the glyphosate application at the dose of 720 g a.e. ha-1....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Herbicide; LC-MS/MS; Metabolites; Metabolic profile.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000200295
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Growth performance and metabolic response of Nile tilapia fed rations supplemented with autolized yeast and zinc R. Bras. Zootec.
Signor,Altevir; Pezzato,Luiz Edivaldo; Padilha,Pedro de Magalhães; Padovani,Carlos Roberto; Barros,Margarida Maria.
This research evaluated the influence of yeast and zinc on growth performance and metabolic responses of Nile tilapia. Diets were formulated to contain 32.0% digestible protein and 3,240 kcal DE/kg diet and the following autolized yeast (%):zinc (mg/kg)relationships: 0.0:0.0; 0.0:79.5; 2.0:0.0; 0.795:79.5; 2.0:200; 4.0:400; 6.0:600; 12.0:1,200 and 14.0:1,400. It was used 135 fingerlings (7.27 ± 0.19 g), distributed in a complete random design in 27 50-L aquaria and they were fed ad libitum four times a day for 128 days. It was evaluated growth performance and metabolic responses, weight gain, apparent feed conversion; protein efficiency and survival rate; percentage of protein, ether extract, dry weight and ashes in the muscle and in the bones; ammonia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fingerlings; Growth; Metabolism; Metabolites; Minerals.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001200002
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Identification of primary and secondary metabolites and transcriptome profile of soybean tissues during different stages of hypoxia. Repositório Alice
COUTINHO, I. D.; MERTZ-HENNING, L. M.; DÖPP, S. A.; NEPOMUCENO, A.; MORAES, L. A. C.; MARCOLINO-GOMES, J.; RICHTER, C.; SCHWALBE, H.; COLNAGO, L. A..
Tipo: Separatas Palavras-chave: Soja; Tecnologia Nuclear; Metabolismo; Soybeans; Nuclear magnetic resonance spectroscopy; Secondary metabolites; Metabolites.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102208
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Identification of secondary metabolites in Cordia verbenacea L.-Boraginaceae. Repositório Alice
LAMEIRA, O. A.; CARDOSO, M. das G.; PINTO, J. E. B. P.; BLANK, M. de F. A.; ALVES, S. de M..
Tipo: Parte de livro Palavras-chave: Metabolitos secundarios; Cordia verbenacea; Plants; Fisiologia Vegetal; Planta; Metabolites; Plant physiology.
Ano: 1997 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/394385
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ISOLATION AND CHARACTERIZATION OF NATURAL HERBICIDAL COMPOUND FROM Drechslera rostrata Planta Daninha
AKBAR,M.; IQBAL,M.S.; KHALIL,T..
ABSTRACT In agriculture, weeds have inevitable importance because of reducing the crop growth and also affecting food quality. Although, synthetic herbicides are available to combat these weeds but during recent years there is a trend of using ecofriendly herbicides extracted from natural resources. Present investigation is a continuity of the research, which reported a natural herbicidal compound named as Ophiobolin A from a fungus, Drechslera rostrata. The fungus was incubated in a growth medium of known composition (minimal medium) up to 28 days and its metabolites were extracted with organic solvents. The compound showing bioactivity was purified with the help of Reversed Phase High Performance Liquid Chromatography (RPHPLC) and identified with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chenopodium album; Natural herbicide; Metabolites.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100254
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Ligninolytic fungus Polyporus sp. S133 mediated metabolic degradation of fluorene BJM
Lazim,Zainab Mat; Hadibarata,Tony.
ABSTRACT This study aimed to investigate the impact of nonionic surfactants on the efficacy of fluorine degradation by Polyporus sp. S133 in a liquid culture. Fluorene was observed to be degraded in its entirety by Polyporus sp. S133 subsequent to a 23-day incubation period. The fastest cell growth rate was observed in the initial 7 days in the culture that was supplemented with Tween 80. The degradation process was primarily modulated by the activity of two ligninolytic enzymes, laccase and MnP. The highest laccase activity was stimulated by the addition of Tween 80 (2443 U/L) followed by mixed surfactant (1766 U/L) and Brij 35 (1655 U/L). UV-vis spectroscopy, TLC analysis and mass spectrum analysis of samples subsequent to the degradation process in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metabolites; Ligninolytic enzymes; Polyporus sp. S133; Non-ionic surfactants; Solubilization.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300610
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Metabolic responses and β-carotene production by the unicellular green alga Dunaliella salina exposed to leaf extracts Acta Botanica
Einali,Alireza; Mazang-Ghasemi,Sahar; Valizadeh,Jafar; Noorozifar,Meisam.
ABSTRACT The present work investigated the effects of aqueous extracts of eucalyptus ( Eucalyptus globulus) and elderberry ( Sambucus ebulus) leaves on β-carotene productivity in Dunaliella salina, a green microalga. Leaf extracts from eucalyptus have greater amounts of phenolics and flavonoids, as well as greater ferric reducing antioxidant potential than elderberry. The extracts of both species greatly inhibited growth of algal suspensions. However, chlorophyll and β-carotene concentration increased in cells treated with leaf extracts, and the highest values were detected in 1 % eucalyptus and 2 % elderberry extracts. Fresh weight, total sugar, and protein content significantly increased following exposure of cells to different doses of leaf extracts....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-carotene; Dunaliella salina; Eucalyptus; Elderberry; Leaf extracts; Metabolites; Stress.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000200180
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Milk production in Saanen goats treated with repeated low doses of intermediate-release insulin during early lactation Ciência Rural
Menezes,Erika Bezerra de; Salles,Maria Gorete Flores; Silva,Cleidson Manoel Gomes da; Fernandes,César Carneiro Linhares; Galeati,Giovanna; Araújo,Airton Alencar de; Rondina,Davide.
ABSTRACT: The effect of insulin administration on the productive responses of Saanen goats during early lactation was investigated. Ten of 20 adult females were subjected to subcutaneous administration of intermediate-acting insulin (0.14UI/kg body weight) at 2, 9, and 14 days postpartum. Milk yield was measured twice daily for 13 weeks and milk samples were collected to measure protein and fat contents. Plasma levels of progesterone, insulin, non-esterifies fatty acids, glucose and other metabolites were measured. Results showed a significantly increased effect of insulin treatment on the content of milk fat and protein; moreover, milk production in the first and second postpartum weeks were higher than control group. The peak of lactation in the insulin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Goat; Insulin; Milk; Postpartum; Metabolites.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000300652
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Otimização do processo não invasivo e não destrutivo de extração e análise de metabólitos voláteis de mamão papaia por SPME-GC-MS. Repositório Alice
TERRA. L. R.; FERREIRA, M. M. C.; TERAO, D.; QUEIROZ, S. C. do N. de.
Abstract: The analysis of volatiles is an alternative in the routine of quality control of fruits, since the volatile metabolites can change in the presence of microorganisms. A well-established method for extracting volatiles is the solid phase microextraction (SPME). This work aims to define some parameters (fiber coating, conditioning time and fiber exposure time) for an effective extraction. Whole papaya was inserted into a hermetically closed glass bottle and the system was left in conditioning for the volatiles accumulation. Then, extraction by SPME and subsequent desorption in a gas chromatograph was performed. Two fibers were evaluated: a 100 μm polydimethylsiloxane (PDMS) and a 65 μm polydimethylsiloxane/divinylbenzene...
Tipo: Artigo de periódico Palavras-chave: Mamão; Carica Papaya; Análise Química; Cromatografia Gasosa; Espectrometria; Papayas; Chemical analysis; Metabolites; Solid phase microextraction; Gas chromatography; Mass spectrometry.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127193
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Photo-protective compounds in red macroalgae from Brittany: Considerable diversity in mycosporine-like amino acids (MAAs) ArchiMer
Lalegerie, Fanny; Lajili, Sirine; Bedoux, Gilles; Taupin, Laure; Stiger-pouvreau, Valerie; Connan, Solène.
To cope with the biotic and abiotic stresses experienced within their environment, marine macroalgae have developed certain defence mechanisms including the synthesis of photo-protective molecules against light and particularly harmful UV radiation. The aim of this study was to screen selected red algae, a highly diverse phylogenetic group, for the production of photo-protective molecules. The pigment content and composition (i.e. chlorophyll-a, phycobiliproteins and carotenoids) and the composition of mycosporine-like amino acids (MAAs) were studied in 40 species of red macroalgae collected in Brittany (France), at two distinct periods (i.e. February and July 2017). A high inter-specific variability was demonstrated in terms of pigment content and MAA...
Tipo: Text Palavras-chave: Algae; Diversity; HPLC; MAAs; Metabolites; Photo-protection; Pigments; Rhodophyta; Screening.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00488/59929/63133.pdf
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Production of Cellulose and Profile Metabolites by Fermentation of Glycerol by Gluconacetobacter Xylinus BABT
Vidotto,Francielle Lina; Ribeiro,Geovana Piveta; Tischer,Cesar Augusto.
ABSTRACT Because of the widespread occurrence of cellulose in nature, many organisms use glycerol as a source of carbon and energy, so these organisms have drawn attention to the potential use of glycerol bioconversion. The bacteria Gluconacetobacter xylinus, a strictly aerobic strain that performing incomplete oxidation of various sugars and alcohols to cellulose biosynthesis. For this reason, we modify the Hestrim-Schram medium, associating glycerol, glucose and sucrose varying their concentration. The fermentations were performed statically at a temperature of 28˚C for a period of 10 days. The pH, membrane formation, crystallinity and the production of some metabolites of the 4th, 7th and 10th days was evaluated. The results showed a higher yield of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial cellulose; Glycerol; Metabolites; Gluconacetobacter xylinus; Solid state nuclear magnetic resonance; 1H nuclear magnetic ressonance.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100404
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Protein, energetic, enzymatic and mineral profile of Nellore cows during the pregnancy, parturition and postpartum Animal Sciences
Oliveira, Wagner Dias Coelho de; Silva, Tairon Pannunzio Dias e; Bezerra, Leílson Rocha; Araújo, Marcos Jácome de; Edvan, Ricardo Loiola; Lopes Oliveira, Ronaldo.
The aim of this study was to evaluate the protein energetic, enzymatic and mineral profile of Nellore cows during the pregnancy, parturition and postpartum. Fifteen multiparous cows with 4 ± 1 years of age and live weight of 400 ± 50 kg were used at different stages (non-pregnant, in the initial, middle and late pregnancy, at birth, one day postpartum, 30 and 60 days postpartum). Blood collections were performed every 30 days and assayed for the following blood biomarkers: Protein (total proteins, albumin, urea and creatinine), energetic (cholesterol, triglycerides, glucose and beta hydroxybutyrate), mineral (calcium, phosphorus, magnesium) and enzymatic (alkaline phosphatase and aspartate aminotransferase). Calcium had the lowest concentrations (p...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biochemistry; Blood; Ketone bodies; Metabolites; Physiology. biochemistry; Blood; Ketone bodies; Metabolites; Physiology..
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/49022
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