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Registros recuperados: 9
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Brucella canis infection in dogs attended in veterinary clinics from patos, Paraíba state, Brazil BJM
Fernandes,Annielle Regina Fonseca; Azevedo,Sérgio Santos de; Piatti,Rosa Maria; Pinheiro,Eliana Scarcelli; Genovez,Margareth Élide; Azevedo,Adílio Santos de; Batista,Carolina de Sousa Américo; Alves,Clebert José.
To determine the frequency of anti-Brucella canis antibodies in dogs attended in veterinary clinics from Patos, Paraíba State, Brazil, as well as to identify risk factors and to isolate and identify the agent, 193 dogs were used. Agar gel immunodiffusion test (AGID) was used to detect B. canis antibodies in sera. Isolation of B. canis was carried out in blood and bone marrow from seropositive animals. Six animals tested seropositive in AGID, resulting in a frequency of 3.11%. B. canis was isolated from bone marrow of one seropositive animal, with confirmation by PCR. Lack of cleaning of the dog's environment was identified as risk factor (odds ratio = 7.91). This is the first report of isolation of B. canis in dogs from the Northeast region of Brazil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diseases; Canine brucellosis; Prevalence; Risk factors; Microbiological culture.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400023
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Comparaison de methodes directes (microscopiques) et indirectes (par mise en culture) dans l'evaluation d'une pollution bacterienne d'origine fecale. Etude preliminaire ArchiMer
Munro, P; Bianchi, M.
Cells of two serotypes of Escherichia coli (O26B6 and 0111B4) were counted using epifluorescence and immunofluorescence microscopy (nonavalent serum) and plate count on specific medium. Values from indirect counts were 10 fold smaller than those from both microscopic counts. In a sample of sea water from an area receiving a fecal pollution, the highest number of bacteria was obtained by epifluorescence microscopy. In that case, the number of cells counted by immunofluorescence microscopy was 10 fold smaller than the number of bacteria able to grow on the medium. It could be due to: the diversity of the natural community, the possibility to other bacteria than E. coli to grow on the used culture medium, the restrictiveness of the serum for "pathogenic...
Tipo: Text Palavras-chave: Escherichia coli; Bacteria; Microbiological culture; Pathogenic bacteria; Microbial contamination; Bacterial counters; Fluorescence microscopy; Bacteria collecting devices; Indicator species.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1001.pdf
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Determination du profil des flores bacteriennes des zones littorales. Application a l'evaluation de l'impact des rejets ArchiMer
Bunetel, L; Segui, A; Guerin, J; Lerman, I; Cormier, M.
The principle of this method is to evaluate, by counts exercised upon selective media, the relative importance of the different bacteria families in the flora of the coastal waters. It is important to separate the contaminant species and the commensal species in the marine environment. Ten culture media distributed into four categories (selective, non selective, saline and non saline) were examined by numeration. Counts were transferred on a diagram allowing to plot down a profile of bacterial flora of water sampled from a given geographical area. The profiles are arranged by a classification method. The sampling points with similar or adjoining profiles were assembled and marked on the chart. In this way, the authors obtained areas representing the impact...
Tipo: Text Palavras-chave: Bacteria; Coastal waters; Microbiological culture; Sewage disposal; Microbial contamination; Bacterial counters; Bacteria collecting devices.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1004.pdf
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Experimental systems for the study of bacterial degradation of pollutants from the oil industry. ArchiMer
Wardell, J; Brown, C.
The release of pollutants into the environment, either during normal operations or due to accidental discharges, has focussed attention on the fate and effects of xenobiotic compounds in the marine environment. This interest has been heightened by the development of the off-shore industry with the subsequent discharge of biocides from platforms. This paper describes the results obtained from investigations into the fate of biocides in the marine environment using continuous-culture techniques.
Tipo: Text Palavras-chave: Bacteria; Continuous culture; Microbiological culture; Oil and gas industry; Pollutants; Pesticides; Biodegradation.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1018.pdf
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Interactions entre les bacteries et les algues dans une culture continue de phytoplancton naturel soumise aux conditions exterieures ArchiMer
Gauthier, M; Martin, Y; Lelong, P; Breittmayer, V.
During an experimental continuous culture of natural marine phytoplankton in environmental conditions, microbial and biochemical tests performed to study production of antibacterial and antialgal substances by unicellular algae. Some parameters were further monitored to characterize bacteria and phytoplankton growth. The presence of inhibitors of algal origin was studied both in the water and in the algal biomass at different phases of the culture, through bioassays performed with bacteria (marine pseudomonads and vibrios, and telluric strains) and several algal strains. Interpretation of the results is difficult. Two successive phases appeared during the growth of phytoplankton, with two different algal populations. The unsteady population of phase I was...
Tipo: Text Palavras-chave: Bacteria; Algae; Microbiological culture; Heterotrophic organisms; Aerobic bacteria; Phytoplankton culture; Sea water; Ecological associations.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-984.pdf
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Isolement et identification de vibrions halophiles d'une station experimentale de mariculture ArchiMer
Bruni, V; Crisafi, E; Maugeri, T; Zaccone, R.
110 Vibrio strains were isolated, at various times of the year, from samples of sea-water supply, breeding-tank water and discarded fish used as food at the breeding stations. From the results obtained by the biochemical and cultural tests, it was possible to identify most of the isolated strains as Vibrio anguillarum . Strains with intermediate characteristics, above-all positive saccharose, proved to be numerous. The authors discuss the occurrence, in particular environments such as those being studied, of atypical or intermediate vibrio strains which are difficult to classify.
Tipo: Text Palavras-chave: Vibrio; Microbiological culture; Bacteria; Food; Minced products; Fish ponds; Sea water; Identification; Marine aquaculture; Bacteria collecting devices; Microbiological analysis.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-997.pdf
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Microbial extracellular enzyme detection on agar plates by means of fluorogenic methylum-belliferyl-substrates. ArchiMer
Kim, S; Hoppe, H.
A rapid and sensitive method to detect the extracellular enzymatic activity of bacteria colonies grown on agar plates is described. Selective agar media supplemented with protein, starch, chitin, Tween-80, etc. are conventionally used to detect biochemical properties of bacteria. It has been experimentally demonstrated with bacteria pure cultures that fluorogenic Methylumbelliferyl (MUF)-substrates are excellent substrate analogues for normally occurring polymers. Based on MUF-substrate hydrolysis the new method provides reliable qualitative estimates of extracellular enzymatic properties of bacteria within minutes using pure cultures as well as agar plates prepared for colony counts.
Tipo: Text Palavras-chave: Bacteria; Microbiological culture; Biochemical analysis; Enzymatic activity; Analytical techniques.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-959.pdf
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Nutrition experimentale: Problemes methodologiques lies a l'utilisation des microorganismes comme source trophique ArchiMer
Guidi, L.
The present study deals with technical problems encountered under lab conditions in studies of trophic relations between deposit-feeding invertebrates and microorganisms. Using 2 pure cultures of Pseudomonas and Navicula , it was shown that these microorganisms will attach to artificial (glass beads) and natural (sand grains) substrates. Best microbial attachment was observed on sand (up to 4.8 x 10 super(8) cells.g super(-1) dry wt.). The radioactive labelling of the microorganisms depends not only on the form under which the isotope ( super(14)C) is supplied to the cultures, but also on the time and the duration of the labelling. 70% of the isotope was incorporated in a stable manner by the diatoms. The bacteria incorporated, at most, 34% of the...
Tipo: Text Palavras-chave: Navicula; Pseudomonas; Bacillariophyceae; Algae; Bacteria; Biological attachment; Radioactive tracers; Methodology; Microorganisms; Trophic relationships; Microbiological culture; Zoobenthos; Nutrition.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-987.pdf
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Plasmid-associated phenathrene degradation by Chesapeake Bay sediment bacteria. ArchiMer
Okpokwasili, G; Somerville, C; Grimes, D; Colwell, R.
A differential, phenanthrene-enrichment agar plating technique was used to isolate phenanthrene-degrading bacteria from phenanthrene-enriched Chesapeake Bay sediment. One of the isolates, a yellow pigmented, slime-producing, Gram-negative rod identified as Flavobacterium sp. has been studied in detail. It has been found to carry a single plasmid with a mass of about 34 megadaltons. Results of hydrocarbon adherence tests showed that the organism adhered only minimally to n-octane and n-hexadecane, but emulsified cyclohexylbenzene and 1,2,3,4,-tetrahydronaphthalene (tetralin). Curing of the plasmid with 3 mu g/ml novobiocin resulted in loss of phenanthrene clearing ability. Ability to degrade phenanthrene was confirmed using super(14)C-labeled phenanthrene.
Tipo: Text Palavras-chave: Flavobacterium; Microbiological culture; Culture media; Bacteria; Sediment pollution; Biodegradation; Bacteria collecting devices.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1014.pdf
Registros recuperados: 9
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