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Registros recuperados: 9
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Biomonitoring of microcystin and aflatoxin co-occurrence in aquaculture using immunohistochemistry and genotoxicity assays BABT
Hashimoto,Elisabete Hiromi; Kamogae,Márcia; Vanzella,Tatiana Perez; Cólus,Ilce Mara Syllus; Bracarense,Ana Paula Frederico Rodrigues Lourenço; Bittencourt-Oliveira,Maria do Carmo; Itano,Eiko; Kuroda,Emília Kiyomi; Kato,Hajime; Nagata,Satoshi; Ueno,Yoshio; Harada,Ken-Ichi; Hirooka,Elisa Yoko.
This work investigated the effects of co-occurring aflatoxin B1 (AFB1) and microcystin (MC) in aquaculture, using immunohistochemistry and genotoxicity methods. Tilapia (Oreochromis niloticus) were exposed to AFB1 by intraperitoneal and MC (cell extract of Microcystis aeruginosa) by intraperitoneal and immersion routes. The interaction of MC-AFB1 was evaluated co-exposing the intraperitoneal doses. Blood samples were collected after 8, 24, and 48h to analyze the micronucleus frequency and comet score. The interaction of MC-AFB1 showed a synergic mutagenic response by higher micronucleus frequency of co-exposed group. A slight genotoxic synergism was also observed in the comet score. Immunohistochemistry detected MC in al lthe fish liver tissues exposed to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microcystis aeruginosa; Mycotoxin and tilapia.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100019
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Changes in the phytoplankton of Lake Planetario after a restoration process Darwiniana
Ehrenhaus,Constanza; Vigna,María Susana.
Lake Planetario is an urban recreational lake that suffered a Microcystis aeruginosa bloom in March 1999. At the same time the birds and fishes that inhabited the lake died, probably affected by microcystin, hepatotoxin that some strains of Miycrocyistis produce. The City of Buenos Aires requested Aguas Argentinas to restore the lake. Restoration tasks included treatment of sediments and pumping of water from a brackish aquifer. For one year, monthly samples were taken with a phytoplankton net, the qualitative and quantitative composition of the phytoplankton were studied, and physicochemical data were collected. The structure and composition of the phytoplankton were studied and revealed that due to the turbulence caused by the pumping of water, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Argentina; Buenos Aires; Bloom; Microcystis aeruginosa; Restoration; Urban recreational lake.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0011-67932006000200002
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Colorimetric test for the monitoring of microcystins in cyanobacterial culture and environmental samples from southeast - Brazil BJM
Almeida,Vanessa P.S.; Cogo,Karina; Tsai,Siu M.; Moon,David H..
Microcystins are hepatotoxic heptapeptides produced by some cyanobacterial genera under determined physico-chemical conditions in the environment, which are responsible for the intoxication and death of animals and humans. The detection of microcystins in potable water or recreational water is not carried out routinely in the majority of Brazilian states. The protein phosphatase 1 (PP1) inhibition test is a simple, rapid and reproducible colorimetric method. The applicability of the PP1 inhibition test was tested using Microcystis aeruginosa (strain 1, UFRJ- toxin producer) grown under controlled light and temperature condition (12/12h light/dark using 30 muE.m².s-1 at 23ºC) in a bioreactor. The total concentrations of P (24, 6 and 4 muM) and Fe (4 and 1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphatase protein; Microcystis aeruginosa; Inhibition and environmen.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200017
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Comparative proteomics between natural Microcystis isolates with a focus on microcystin synthesis. Repositório Alice
TONIETTO, A.; PETRIZ, B. A.; ARAÚJO, W. C.; REIS, A. M. dos; MAGALHÃES, B. S.; FRANCO, O. L..
bitstream/item/156255/1/art3A10.11862F1477-5956-10-38.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cyanotoxin; Cyanobacteria; Microcystis aeruginosa; Proteome.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/950118
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Hepatosplenomegaly caused by an extract of cyanobacterium Microcystis aeruginosa bloom collected in the Manguaba Lagoon, Alagoas - Brazil Rev. Microbiol.
Porfirio,Zenaldo; Ribeiro,Micheline P.; Estevam,Cicero S.; Houly,Ricardo L. S.; Sant'Ana,Antonio Euzébio G..
Cyanobacteria (Microcystis aeruginosa), which produce powerful hepatotoxic cyclopeptides, were collected and submitted to the determination of toxicity through intraperitoneal injections made in 30 and 90 days-old Swiss albino mice. The liver and the spleen were histopathologically analyzed and the weight and vital signs development were monitored. Test of toxicity resulted in a LD50 of 154.28 mg.Kg-1. M. aeruginosa represented 95% of the analyzed biomass. The ratios between liver weight and body weight in the animal inoculated with a single dose were 6.0% and 7.2%, with multi doses 7.0% and 7.5% and in the control animals 4.0% and 5.0%, for adult and young animals, respectively. There was an accentuated increase in the volume and weight of the spleen, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microcystis aeruginosa; Cyanobacteria; Hepatomegaly; Splenomegaly.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300016
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Occurrence of toxic blooms of Microcystis aeruginosa in a central Chilean (36° Lat. S) urban lake RChHN
Almanza,Viviana; Parra,Oscar; Bicudo,Carlos E. De M.; Baeza,Carolina; Beltran,Johana; Figueroa,Ricardo; Urrutia,Roberto.
BACKGROUND: During the last decades the frequency and global distribution of toxic cyanobacteria blooms has increased globally, which has been attributed to the eutrophication and climate change. In Chile there have been reports on blooms in aquatic ecosystem in localities with high density population and on the presence of five congeners of microcystins but only two documented toxics blooms with hundreds fish kills. We investigated the presence of toxic cyanobacteria blooms in the Lo Galindo urban lake, Concepción city, and the environmental factors that influence the abundance of cyanobacteria and microcystins concentration. Lo Galindo Lake, is used for various recreational and eventually as a drinking water source RESULTS: Toxic blooms of Microcystis...
Tipo: Journal article Palavras-chave: Microcystin; Microcystis aeruginosa; Scums blooms; Dispersive blooms; Eutrophication; Urban lake; Chile.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2016000100008
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Ocorrência, distribuição e toxicidade de cianobactérias no estuário da Lagoa dos Patos, RS BJB
MATTHIENSEN,A.; YUNES,J. S.; CODD,G. A..
Diversas florações da cianobactéria Microcystis aeruginosa têm sido observadas nas águas do estuário da Lagoa dos Patos nos últimos 15 anos, sem o devido estudo de sua distribuição e ocorrência ou do risco de contaminação do ecossistema aquático. Neste estudo, foram realizadas a identificação e a quantificação das cianobactérias da região estuarina da Lagoa dos Patos, particularmente M. aeruginosa, incluindo um levantamento quali-quantitativo dos principais grupos microfitoplanctônicos em relação à sua distribuição geográfica no estuário. Evidenciou-se que, ao longo de 12 meses, Microcystis aeruginosa esteve presente na região estuarina, com valor máximo de 1,3.10(6)cél.L-1 em dezembro de 1994 e mínimo de 1,5.10(5) cél.L-1 em agosto de 1995, evidenciando a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cianobactéria; Microcystis aeruginosa; Hepatotoxinas; Estuário da Lagoa dos Patos; Microcistinas.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71081999000300002
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Release of available nitrogen from river-discharged dissolved organic matter by heterotrophic bacteria associated with the cyanobacterium Microcystis aeruginosa ArchiMer
Purvina, S; Béchemin, Christian; Balode, M; Verite, Celine; Arnaud, Christophe; Maestrini, Serge Y.
The use of riverine dissolved organic matter by the heterotrophic bacteria associated with a culture of the cyanobacterium Microcystis aeruginosa and release of simple nitrogen compounds were studied in an experimental series. Bacteria reduced the bulk of dissolved organic nitrogen (DON) by half, but when associated with M. aeruginosa, DON was excreted and its concentration rose by 13%. During the stationary growth phase bacteria released ammonium, doubling the concentration of ammonia as well as of nitrates. Bacteria associated with M. aeruginosa consumed riverine DON and joined the ammonification and nitrification process, supplying cyanobacteria with simple nitrogen compounds.
Tipo: Text Palavras-chave: Microcystis aeruginosa; Heterotrophic bacteria; DOM; DON; Nitrates; Ammonia.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00353/46408/46122.pdf
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Salt Shock Responses of Microcystis Revealed through Physiological, Transcript, and Metabolomic Analyses ArchiMer
Georges Des Aulnois, Maxime; Réveillon, Damien; Robert, Elise; Caruana, Amandine; Briand, Enora; Guljamow, Arthur; Dittmann, Elke; Amzil, Zouher; Bormans, Myriam.
The transfer of Microcystis aeruginosa from freshwater to estuaries has been described worldwide and salinity is reported as the main factor controlling the expansion of M. aeruginosa to coastal environments. Analyzing the expression levels of targeted genes and employing both targeted and non-targeted metabolomic approaches, this study investigated the e ect of a sudden salt increase on the physiological and metabolic responses of two toxic M. aeruginosa strains separately isolated from fresh and brackish waters, respectively, PCC 7820 and 7806. Supported by di erences in gene expressions and metabolic profiles, salt tolerance was found to be strain specific. An increase in salinity decreased the growth of M. aeruginosa with a lesser impact on the...
Tipo: Text Palavras-chave: Microcystis aeruginosa; Microcystin; Salt stress; Metabolomic; Transcript.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00615/72689/71692.pdf
Registros recuperados: 9
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