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Registros recuperados: 28
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Anil-Babesia rodhaini Monoclonal Antibodies: Effect against Babesia rodhaini and Cross-reactivity with Human Starin of Babesia microti OAK
Claveria, Florencia G.; Omata, Yoshitaka; Takehara, Tokuma; Saito, Atsushi; Maki, Yoshiyuki; Igarashi, Ikuo; Suzuki, Naoyoshi.
Anti-Babesia rodhaini monoclonal antibodies (mAb), namely: 1-E7, 2-H2 and 3-B8, significantly suppressed the development of high parasitemia in BALB/c mice infected with B. rodhaini and all mAb-treated mice survived the infection. While, only monoclonal antibody 3-B8 showed some inhibitory effect against Babesia microti, with mice showing high parasitemia of 18.04 ± 2.69 %, at 9 days post-exposure. Westernblot analysis of B. rodhaini and B. microti parasite extract reacted with anti-B. rodhaini monoclonal antibodies showed cross-reactive bands of molecular weights 62 and 55 kilodaltons. Comparison of the antigenic components of B. rodhaini and B. microti using polyspecific sera revealed several shared or common parasite antigens of molecular weights 62,...
Palavras-chave: Monoclonal antibodies; Cross-reactivity; Babesia rodhaini; Babesia microti.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/171
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Antigenic characterization of Brazilian bovine viral diarrhea virus isolates by monoclonal antibodies and cross-neutralization BJMBR
Botton,S.A.; da-Silva,A.M.; Brum,M.C.S.; Weiblen,R.; Flores,E.F..
Nineteen Brazilian isolates of bovine viral diarrhea virus (BVDV) were characterized antigenically with a panel of 19 monoclonal antibodies (mAbs) (Corapi WV, Donis RO and Dubovi EJ (1990) American Journal of Veterinary Research, 55: 1388-1394). Eight isolates were further characterized by cross-neutralization using sheep monospecific antisera. Analysis of mAb binding to viral antigens by indirect immunofluorescence revealed distinct patterns of reactivity among the native viruses. Local isolates differed from the prototype Singer strain in recognition by up to 14 mAbs. Only two mAbs - one to the non-structural protein NS23/p125 and another to the envelope glycoprotein E0/gp48 - recognized 100% of the isolates. No isolate was recognized by more than 14...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine viral diarrhea virus; BVDV; Monoclonal antibodies; Cross-neutralization; Antigenic diversity.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100011
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Antigenic Differences Between Blastocystis hominis and Blastocystis sp. Revealed by Polyclonal and Monoclonal Antibodies OAK
Yoshikawa, Hisao; Katagiri, Izumi; Li, Xiaohai; Becker-Hapak, Michelle; Graves, Don C..
Palavras-chave: Antigenic differences; Polyclonal antibodies; Monoclonal antibodies; Blastocystis hominis; Blastocystis sp..
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/232
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Can anti-bothropstoxin-I antibodies discriminate between Bothrops jararaca and Bothrops jararacussu venoms? J. Venom. Anim. Toxins incl. Trop. Dis.
Araujo,Ricardo Teixeira; Corrêa-Netto,Carlos; Brazil-Más,Leonora; Silveira,Caio Raony Farina; Fernandes,Irene; Zingali,Russolina Benedeta.
Abstract Background Snakes of the genus Bothrops, popularly known as pit vipers, are responsible for most cases of snakebite in Brazil. Within this genus, Bothrops jararacussu and B. jararaca deserve special attention due to the severity of their bites and for inhabiting densely populated areas. Regarding the treatment of snakebites by Bothrops jararacussu, questions have been raised about the effectiveness of the specific bothropic antivenom in neutralizing myotoxic effects; however, there are no accurate data for humans. Thus, the development of a differential diagnostic kit for this species would be of great interest because it provides, for healthcare professionals, a tool that would allow us to determine whether the accident was caused by B....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bothrops jararacussu; Bothrops jararaca; Bothropstoxin-I; Monoclonal antibodies; Species specificity.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100308
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Caractérisation morphologique et fonctionnelle des hémocytes chez l'huître plate, Ostrea edulis ArchiMer
Xue, Quiggang.
The mechanism that bivalve molluscs react to infectious agents are important research subjects in the fIeld of disease control studies for these animals. While the selection of disease resistant populations have made decisive progression in some species, the mechanism behinds these resistant phenomena is not yet clear so far. However, hemocytes must represent one of the key factors in the reaction process. Based on this hypothesis, the structure and function of European flat oyster, Ostrea edulis, hemocytes have been investigated in aid of different techniques in the present study. ln addition, Pacific oyster, Crassostrea gigas, hemocytes have been analysed in certain aspects so as to provide some results for comparison. European flat oyster and Pacific...
Tipo: Text Palavras-chave: Bivalvia; Ostrea edulis; Crassostrea gigas; Mécanismes de défense; Hémocytes; Granulocytes; Hyalinocytes; Séparation; Anticorps monoclonaux; Enzymes; Cytométrie de flux; Bivalvia; Ostrea edulis; Crassostrea gigas; Defence mechanisms; Hemocytes; Granulocytes; Hyalinocytes; Separation; Monoclonal antibodies; Enzymes; Flow cytometry.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00107/21820/19406.pdf
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Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin Biol. Res.
AVILA,ANA D; CALDERÓN,CARLOS F; PEREZ,RITA M; PONS,CARMEN; PEREDA,CELIA M; ORTIZ,ANA R.
Hybrid molecules obtained through conjugation of monoclonal antibodies and toxins constitute an approach under exploration to generate potential agents for the treatment of cancer and other diseases. A frequently employed toxic component in the construction of such immunotoxins is ricin, a plant toxin which inhibits protein synthesis at ribosomal level and so requires to be internalized by the cell. A hemolytic toxin isolated from the sea anemone Stichodactyla helianthus, which is active at the cell membrane level, was linked through a disulfide bond to the anti-epidermal growth factor receptor monoclonal antibody ior egf/r3. The resulting immunotoxin did not exhibit hemolytic activity except under reducing conditions. It was toxic for H125 cells that...
Tipo: Journal article Palavras-chave: Epidermal growth factor; Epidermal growth factor receptor; Hemolytic toxins; Immunotoxins; Monoclonal antibodies.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200008
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Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection BJID
Paulini,Inarei; Siqueira-Silva,Joselma; Thomaz,Luciana; Rocha,Leticia; Harsi,Charlotte; Bellei,Nancy; Granato,Celso.
Abstract Human adenoviruses comprise an important group of etiologic agents that are responsible for various diseases in adults and children, such as respiratory, ocular, gastroenteric, and urinary infections. In immunocompromised and organ-transplanted individuals, these agents can cause generalized infections. Rapid diagnostic methods for detecting these infectious agents are not widely available.The aim of this work was to produce monoclonal and polyclonal anti-adenovirus antibodies to be used in a rapid diagnostic test for respiratory infections.Adenovirus hexons were satisfactorily purified by ultracentrifugation and chromatography. After virus purification, anti-hexon monoclonal antibodies were produced and characterized, following classical methods....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenovirus; Monoclonal antibodies; Immunochromatographic test.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500500
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Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster: Biocell
Noher de Halac,I.; Cismondi,I.A.; Rodriguez-Garcia,M.I.; Famá,G..
Cell wall pectins are some of the most complex biopolymers known, and yet their functions remain largely mysterious. The aim of this paper was to deepen the study of the spatial pattern of pectin distribution in the aperture of Oenothera hookeri.velans ster/+ster fertile pollen. We used "in situ" immunocytochemical techniques at electron microscopy, involving monoclonal antibodies JIM5 and JIM7 directed against pectin epitopes in fertile pollen grains of Oenothera hookeri.velans ster/+ster. The same region was also analyzed by classical cytochemistry for polysaccharide detection. Immunogold labelling at the JIM7 epitope showed only in mature pollen labelling mainly located at the intine endo-aperture region. Cytoplasmic structures near the plasma membrane...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectins; Monoclonal antibodies; JIM5; JIM7; Immunogold; Pollen; Sporoderm; Oenothera; Cytochemistry.
Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100002
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Etude d'une prolifération hémocytaire chez la moule Mytilus edulis ArchiMer
Noel, Danièle.
Hemic proliferative disease in the bay mussel, Mytilus edulis, has been studied following mortalities in Washington State (USA). The disease has been reproduced in the labortory. The establishment of this experimental model led to study tumoral characteristics of the atypic hemocytes : ultrastrure, in vitro cultivation, transplantation. The infectious etiology of this tumor has been verified. It has been hypothesized to result from a viral infection since retrovirus-like particules have been observed following induction by bromodeoxyuridine. Specific monoclonal antibodies against atypic hemocytes have been obtained and characcterized
Tipo: Text Palavras-chave: Mytilus; Mollusque; Hémocyte; Tumeur; Ulstrastructure; In vitro culture; Transplantation; Rétrovirus; Anticorps monoclonaux; Mytilus; Mollusca; Hemocyte; Tumor; Ultrastructure; In vitro cultivation; Transplantation; Retrovirus; Monoclonal antibodies.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00428/53930/55057.pdf
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Expression of class 5 antigens by meningococcal strains obtained from patients in Brazil and evaluation of two new monoclonal antibodies BJID
De Gaspari,Elizabeth N.; Zollinger,Wendell D..
Determining the profile of antigen expression among meningococci is important for epidemiologic surveillance and vaccine development. To this end, two new mouse monoclonal antibodies (MAbs) have been derived against Neisseria meningitidis proteins (class 5). The MAbs were reactive against outer membrane antigens and were bactericidal. Selected anti-class 5 MAbs [(5.1)-3E6-2; (5.3)-3BH4-C7; (5.4)-1BG11-C7; (5.5)-3DH-F5G9 also 5F1F4-T3(5.c)], and the two new monoclonal antibodies C14F10Br2 (5.8) and 7F11B5Br3 (5.9), were then tested against different meningococcal strains, (63 strains of serogroup A, 60 strains of serogroup C (from 1972 to 1974); and 136 strains of serogroup B (from 1992) meningococci). Our results demonstrated that the expression of class 5...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neisseria meningitidis; Monoclonal antibodies; Outer membrane protein; Flow cytometry.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000300007
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Glycosphingolipid antigens from Leishmania (L.) amazonensis amastigotes: Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivo BJMBR
Specific glycosphingolipid antigens of Leishmania (L.) amazonensis amastigotes reactive with the monoclonal antibodies (MoAbs) ST-3, ST-4 and ST-5 were isolated, and their structure was partially elucidated by negative ion fast atom bombardment mass spectrometry. The glycan moieties of five antigens presented linear sequences of hexoses and N-acetylhexosamines ranging from four to six sugar residues, and the ceramide moieties were found to be composed by a sphingosine d18:1 and fatty acids 24:1 or 16:0. Affinities of the three monoclonal antibodies to amastigote glycosphingolipid antigens were also analyzed by ELISA. MoAb ST-3 reacted equally well with all glycosphingolipid antigens tested, whereas ST-4 and ST-5 presented higher affinities to...
Palavras-chave: Glycosphingolipids; Monoclonal antibodies; Leishmania (L.) amazonensis; Fast atom bombardment mass spectrometry; Carbohydrate antigens.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300014
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Immune cell profiles associated with resistance against Haemonchus contortus in Morada Nova sheep. Repositório Alice
OKINO, C. H.; TOSCANO, J. H. B.; LOPES, L. G.; SILVA, M. H.; GIRALDELO, L. A.; NICIURA, S. C. M.; BENAVIDES, M. V.; ESTEVES, S. N.; CHAGAS, A. C. de S..
Effective mucosal immune response is essential for the development of resistance to Haemonchus contortus in sheep.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Resistência contra H contortus; Ovelha; Haemonchus Contortus; Monoclonal antibodies; Lysozyme; Eosinophils.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1097251
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Immunocytochemistry of the mucilage cells of Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae) Rev. Bras. Bot.
Mastroberti,Alexandra Antunes; Mariath,Jorge Ernesto de Araujo.
The use of monoclonal antibodies for specific pectic epitopes is an important tool in the study of the cell wall. Throughout the development of mucilage cells of Araucaria angustifolia (Bertol.) Kuntze, a gradient of distribution was observed in relation to the pectic de-esterification, as well as to the increase of galactan and arabinan epitope distribution, and to the reduction of arabinogalactans proteins (AGPs) epitope at maturity. AGP and methyl-esterified homogalacturonan (HGA) were present in the mucilage. Galactans and arabinans were also observed in the mucilage, though with weak labelling. Degradation of AGP in the maturity of mucilage cells, in cell wall, as well as in the secretion, could be involved in the programmed cell death (PCD)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Araucaria angustifolia; Cell development; Cell wall; Monoclonal antibodies; Mucilage cells; Pectin.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042008000100002
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Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira BJM
Lüdtke,Charli Beatriz; Coutinho,Mariana Loner; Jouglard,Sandra Denise Dorneles; Moreira,Cecília Nunes; Fernandes,Cláudia Hartleben Pinho; Brod,Claudiomar Soares; Haake,David A.; Ko,Albert Icksang; Dellagostin,Odir Antonio; Aleixo,José Antonio Guimarães.
Two hybridomas secreting monoclonal antibodies (MAbs) that react with a lipoprotein (LipL32) of the outer membrane of pathogenic Leptospira were obtained. For hybridoma production, spleen cells from BALB/c mice imunized with recombinant LipL32 (rLipL32) were fused to SP2/O-Ag14 cells, selected in HAT medium and screened in an indirect ELISA. One MAb produced was of the IgG2b isotype and the other was an IgM. MAbs specificity was confirmed by indirect ELISA and immunoblotting using purified rLipL32 and whole-cell antigen preparations from Escherichia coli (E. coli) expressing LipL32 and from pathogenic and non-pathogenic serovars. Both Mabs reacted with most of the pathogenic serovars tested and none reacted with non-pathogenic Leptospira. The MAbs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospirosis; Monoclonal antibodies; LipL32.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500001
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Monoclonal antibodies against Bonamia ostreae (Protozoa: Ascetospera), an intrahaemocytic parasite of flat oyster Ostrea edulis (Mollusca : Bivlavia) ArchiMer
Rogier, Hervé; Hervio, Dominique; Boulo, Viviane; Clavies, Christine; Hervaud, Eliane; Bachere, Evelyne; Mialhe, Eric; Grizel, Henri; Pau, Bernard; Paolucci, Francis.
The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These...
Tipo: Text Palavras-chave: Monoclonal antibodies; Oyster; Ostrea edulis; Bonamia ostreae.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-2756.pdf
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Monoclonal antibodies against sporangia and spores of Marteilia sp. (Protozoa: Ascetospora) ArchiMer
Robledo, Jaf; Boulo, Viviane; Mialhe, Eric; Despres, Béatrice; Figueras, Antonio.
Digestive glands of mussels Mytilus edulis from Brittany, France, infected with Marteilia sp. (Ascetospora) were used to purify the parasite. A modification of a previously used purification protocol increased purification efficiency, permitting sporangial primordia and sporangia of Marteilia sp. to be obtained. Mouse (Balb/c) monoclonal antibodies were generated against this parasite. From the fusion, 26 monoclonal antibodies against Marteilia sp. were obtained. Antibodies from 6 clones reacted only with Marteilia sp. cells and not with normal host tissues. Four of these antibodies (1/1-3, 3/1-1, 4/1-1 and 6/2-3) reacted with the sporangia wall and two with the spore cytoplasm (9/1-1 and 12/5-1). Antibodies cross-reacted with Marteilia refringens from...
Tipo: Text Palavras-chave: Marteilia sp.; Mytilus edulis; Monoclonal antibodies.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00196/30730/29404.pdf
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Monoclonal Antibodies Prepared against Theileria annulata-infected Bovine Cells Recognize an Infection-associated Antigen Expressed by the Infected Cells OAK
DÖrflinger, W.; Schein, E.; Ahmed, J. S..
A battery of monoclonal antibodies were produced in mice against Theileria annulata-infected cells. Two of these antibodies (IgM and IgG1) reacted specifically with the surface membrane of the infected cells but not with Con A-stimulated or unstimulated bovine peripheral blood lymphocytes (PBL). The antibodies could stain also clones which were derived from the cell lines tested in the present study. Similarly, they reacted with T.annulata-infected but not with uninfected sheep PBL. After removal of the parasites by treating the infected cells with the anti-theilerial drug buparvaquone, the antibodies were no more able to bind to the cells.
Palavras-chave: Membrane antigen; Monoclonal antibodies; Theileria annulata-infected bovine cells.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/161
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MONOCLONAL ANTIBODIES TO IDENTIFY TOMATO MOSAIC TOBAMOVIRUS (TOMV) BJM
Duarte,Keila M.R.; Gomes,Luiz Humberto; Gesztesi,Jean-Luc; Lopes,José Daniel; Tavares,Flávio C.A..
Monoclonal antibodies were obtained against Tomato mosaic tobamovirus (ToMV) isolated in Brazil. One antibody (8G7G2) isotyped as IgG2b (kappa light chain) showed strong specificity and very low cross reaction with the Tobacco mosaic virus (TMV). It can be used in identification of tomato mosaic virus (ToMV).
Tipo: Info:eu-repo/semantics/other Palavras-chave: Tomato mosaic tobamovirus; ELISA; Monoclonal antibodies.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300016
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Production and caracterization of monoclonal antibodies for the detection of Salmonella enterica in chicken meat BJM
Schneid,Andréa dos Santos; Lüdtke,Charli Beatriz; Diel,Cristiane; Aleixo,José AntonioGuimarães.
A panel of 13 monoclonal antibodies (MAbs) that react against outer membrane proteins of Salmonella Enteritidis was obtained. Two MAbs were classified as IgM, six were IgG2a, three were IgG3 and one was of the IgG2b isotype. The reactivity of the MAbs against different serovars of Salmonella enterica and other bacteria was investigated using an indirect ELISA. Five MAbs reacted only against Salmonella Enteritidis. Two MAbs presented crossed reactions with thermo-extracted antigens of Klebsiella pneumoniae, Citrobacter freundii and Enterobacter aerogenes. MAb 424H presented wide spectrum of reactivity, detecting antigens of Salmonella belonging to serogroups B, C, D, E and G. The detection limit of different serovars of Salmonella in a indirect ELISA with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Monoclonal antibodies; Membrane proteins; Foods.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200012
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Production and characterization of a monoclonal antibody against an Ascaris suum allergenic component BJMBR
Pires,R.R.; Oshiro,T.M.; Itami,D.M.; Fernandes,I.; Macedo-Soares,M.F..
Ascaris suum allergenic components (PIII) separated by gel filtration chromatography of an adult worm extract were used to immunize BALB/c mice. Popliteal lymph node cells taken from the immunized animals were fused with SP2/O myeloma cells using polyethylene glycol (MW 1450) as fusogen. The hybridomas were cultured in HAT-containing medium and cloned at limiting dilutions. Supernatants from the growing hybrids were screened by ELISA using plates coated with PIII or the A. suum crude extract. The monoclonal antibody obtained, named MAC-3 (mouse anti-A. suum allergenic component), is an IgG1 kappa mouse immunoglobulin that specifically recognizes a 29,000 molecular weight protein (called allergenic protein) with an affinity constant of 1.7 x 10(9) M-1. The...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Ascaris suum; Allergen; IgE; Monoclonal antibodies.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800009
Registros recuperados: 28
Primeira ... 12 ... Última
 

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