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Registros recuperados: 45 | |
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Boutet, Isabelle; Ky, Chin Long; Bonhomme, Francois. |
Euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). This physiological capacity is generally monitored using enzyme activity assays (Na+/K+-ATPase...), hormones quantification (prolactine, growth hormone) or their mRNAs expression. To date, few studies addressed the genetic correlates of adaptation to varying salinity at a molecular level in such fish. In the sea bass Dicentrarchus labrax, genetic differentiation was observed at specific allozyme loci between lagoon- and open-sea populations. In the present study, we investigated transcriptomic response of D. labrax to salt- and freshwater acclimation in two organs involved in osmoregulation, gill and intestine. By using... |
Tipo: Text |
Palavras-chave: Suppression subtractive hybridisation; Carbonic anhydrase; Osmoregulation; MRNA expression; Sea bass. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1901.pdf |
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Lisboa,Viviana; Barcarolli,Indianara F.; Sampaio,Luís A.; Bianchini,Adalto. |
Survival and physiological parameters associated with metabolism and osmoregulation were evaluated in juveniles of the Lebranche mullet Mugil liza acclimated to different water salinities (5, 10, 20, 30, and 40‰) for 15 days. Room temperature (25ºC) and photoperiod (12L:12D) were fixed. Fish were fed twice-a-day with commercial diet (28% crude protein) until satiation. After acclimation, whole body oxygen consumption was measured and fish were euthanized and sampled for blood, gills, and liver. Whole body oxygen consumption and plasma osmolality did not change in the range of salinities tested. The isosmotic point was estimated as 412.7 mOsmol kg-1 (13.5‰). Gill Na+,K+-ATPase activity tended to be lower at 20 and 30‰, while liver glycogen content was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish; Glycogen; Na+; K+-ATPase; Osmoregulation; Oxygen consumption. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300591 |
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Amaral,Marcelo da Cunha; Bonecker,Ana Cristina Teixeira; Ortiz,Cláudio H. D.. |
This work aimed to know the tolerance mechanisms through the salinity variation by Na+ K+ - ATPase and Mg++ - ATPase and enzymes encountered in the gills of Poecilia vivipara. In field, the presence of this species was observed in salinities of 0 and 28. In laboratory, these fish were maintained in aquarium with mean salinity of 30 and positive responses were obtained. Some adult specimens, collected in a lagoon of the Coqueiros Beach, were utilized as matrixes. In the experiments the specimens used were those born in the test aquarium. For each salinity studied three replicates were made with three specimens for each one. The alevins were maintained in salinities of 5, 10, 15, 20, 25, 30 and 35 during a month for adaptation. Gills were extracted in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Poecilia vivipara; Na+ K+ ATPase; Mg++ ATPase; Osmoregulation. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100001 |
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Handayani, Kiki Syaputri; Soegianto, Agoes; Lignot, Jehan-hervé. |
The effects of Hg exposure on blood parameters and gill physiology of tilapia (Oreochromis niloticus) were analyzed. Fish maintained in freshwater were exposed for 7 days (d) to sublethal mercury concentrations (0.1 and 1 mg/L). Blood serum osmolality (SO), sodium (Na+), potassium (K+) and chloride (Cl−) ionic concentrations, and hematological parameters were assessed after 1 up to 7 d of exposure. Serum osmolality and ionic concentrations of exposed fish appeared differently affected throughout the experimental period compared to the controls. Osmolality was reduced at the 2 tested concentrations but Na+ and Cl− contents were only altered at 1 mg/L of Hg after 1 d of exposure and values rapidly returned to the control values thereafter. K+ content was... |
Tipo: Text |
Palavras-chave: Mercury; Tilapia; Osmoregulation; Serum ions; Blood; Aquatic toxicology. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00648/76033/76943.pdf |
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Alix, Maud; Gasset, Eric; Bardon-albaret, Agnes; Noel, Jean; Pirot, Nelly; Perez, Valérie; Coves, Denis; Saulnier, Denis; Lignot, Jehan-hervé; Cucchi, Patricia N.. |
Background Ephippidae fish are characterized by a discoid shape with a very small visceral cavity. Among them Platax orbicularis has a high economic potential due to its flesh quality and flesh to carcass ratio. Nonetheless, the development of its aquaculture is limited by high mortality rates, especially due to Tenacibaculum maritimum infection, occurring one to three weeks after the transfer of fishes from bio-secure land-based aquaculture system to the lagoon cages for growth. Among the lines of defense against this microbial infection, the gastrointestinal tract (GIT) is less studied. The knowledge about the morphofunctional anatomy of this organ in P. orbicularis is still scarce. Therefore, the aims of this study are to characterize the GIT in... |
Tipo: Text |
Palavras-chave: Digestive system; Tenacibaculosis; Mucous; Osmoregulation; Absorption; Batfish; Teleost. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00652/76421/77485.pdf |
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Boudour-boucheker, Nesrine; Boulo, Viviane; Charmantier-daures, Mireille; Grousset, Evelyse; Anger, Klaus; Charmantier, Guy; Lorin-nebel, Catherine. |
V-H+-ATPase and Na+/K+-ATPase were localized in the gills and branchiostegites of M. amazonicum and the effects of salinity on the branchial chamber ultrastructure and on the localization of transporters were investigated. Gills present septal and pillar cells. In freshwater (FW), the apical surface of pillar cells is amplified by extensive evaginations associated with mitochondria. V-H+-ATPase immunofluorescence was localized in the membranes of the apical evaginations and in clustered subapical areas of pillar cells, suggesting labeling of intracellular vesicle membranes. Na+/K+-ATPase labeling was restricted to the septal cells. No difference in immunostaining was recorded for both proteins according to salinity (FW vs. 25 PSU). In the branchiostegite,... |
Tipo: Text |
Palavras-chave: Osmoregulation; Na+/K+-ATPase; V-type H+-ATPase; Gills; Branchiostegite. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00202/31352/37901.pdf |
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Giffard, I; Charmantier, G; Grousset, E; Aujoulat, F; Castille, R. |
The ontogeny of the digestive tract (DT) and of Na+/K+-ATPase localization was investigated during the early postembryonic development (from yolk sac larva to juvenile) of the euryhaline teleost Dicentrarchus labrax reared at two salinities: seawater and diluted seawater. Histology, electron microscopy and immunocytochemistry were used to determine the presence and differentiation of ion transporting cells. At hatching, the DT is an undifferentiated straight tube over the yolk sac. At the mouth opening (day 5), it comprises six segments: buccopharynx, esophagus, stomach, anterior intestine, posterior intestine and rectum, well differentiated at the juvenile stage (day 72). The enterocytes displayed ultrastructural features similar to those of... |
Tipo: Text |
Palavras-chave: Osmoregulation; Na+/K+ ATPase; Immunolocalization; Fish larva; Enteric ionocyte. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-4653.pdf |
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Becker,Alexssandro Geferson; Garcia,Luciano de Oliveira; Kochhann,Daiani; Gonçalves,Jamile Fabbrin; Loro,Vania Lúcia; Baldisserotto,Bernardo. |
Ionic contents (Na+, K+ and Cl-) of plasma and gallbladder bile (GB) of juveniles silver catfish, Rhamdia quelen (156.1±0.2g, 28.2±0.3cm), were determined in three different times (0, 6 and 24h) after exposure to: a) control or high dissolved oxygen (DO = 6.5mg L-1) + low NH3 (0.03mg L-1); b) low DO (3.5mg L-1) + low NH3; c) high DO + high NH3 (0.1mg L-1); and d) low DO + high NH3. High waterborne NH3 or low DO levels increased plasma and GB ion levels. These parameters might have followed different mechanisms to affect osmoregulation since a synergic effect of these variables was detected. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nitrogen compound; Hypoxia; Ion levels; Jundiá; Osmoregulation. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000600020 |
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Azevedo Neto,André Dias de; Prisco,José Tarquinio; Enéas-Filho,Joaquim; Lacerda,Claudivan Feitosa de; Silva,José Vieira; Costa,Paulo Henrique Alves da; Gomes-Filho,Enéas. |
Seeds from eight different maize genotypes (BR3123, BR5004, BR5011, BR5026, BR5033, CMS50, D766 and ICI8447) were sown in vermiculite, and after germination they were transplanted into nutrient solution or nutrient solution containing 100 mmol.L-1 of NaCl and placed in a greenhouse. During the experimental period plant growth (dry matter, shoot to root dry mass ratio, leaf area, relative growth rate, and net assimilation rate), leaf temperature, stomatal conductance, transpiration, predawn water potential, sodium, potassium, soluble amino acids and soluble carbohydrate contents were determined in both control and salt stressed plants of all genotypes studied. Salt stress reduced plant growth of all genotypes but the genotypes BR5033 and BR5011 were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Growth analysis; Osmoregulation; Salinity; Transpiration; Zea mays; Water relations. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000100005 |
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HARTMAN BAKKEN,BRADLEY; SABAT,PABLO. |
Nectar-feeding birds oscillate between avoiding overhydration when they are feeding and preventing dehydration during fasts. Here, we examined how resting rates of total evaporative water loss (TEWL) and metabolic water production (MWP) influence water balance in the green-backed firecrown (Sephanoides sephanoides), a Chilean hummingbird. We hypothesized that a circadian rhythm in TEWL would assuage the dehydration risk that hummingbirds face during the night. However, we did not find support for this idea. In resting hummingbirds, rates of TEWL during the day (54 ±6 µLh-1,n = 8) and night (65 ± 12 h-1, n = 5) were similar. Rates of MWP were also similar between the day (22 ±3 µL h-1, n = 8) and night (23 ± 2 µL h-1, n = 5). MWP rates were significantly... |
Tipo: Journal article |
Palavras-chave: Circadian rhythm; Evaporative water loss; Hummingbird; Osmoregulation; Water balance. |
Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2007000300001 |
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Lima,Geovani S. de; Pinheiro,Francisco W. A.; Dias,Adaan S.; Gheyi,Hans R.; Soares,Lauriane A. dos A.; Silva,Saulo S. da. |
ABSTRACT Due to water limitations in terms of both quantity and quality in the semi-arid region of northeastern Brazil, the use of waters with high concentrations of salts become necessary in irrigated agriculture. Thus, this study aimed to evaluate the growth and production of grafted West Indian cherry under saline water irrigation and potassium fertilization. The experiment was carried out in drainage lysimeters under protected environment conditions, using a Regolithic Neosol with sandy loam texture. Treatments resulted from the combination of two levels of electrical conductivity - ECw (0.8 and 3.8 dS m-1) of irrigation water and four doses of potassium (50, 75, 100 and 125% of the recommendation of Musser), arranged in a randomized block design, with... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Malpighia emarginata; Salt stress; Osmoregulation. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662019000400250 |
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MOHAMED,KAMEL. |
The familiar solutes, Na+, K+, Ca2+, Mg2+, Cl-, PO4(3-), SO4(2-), soluble carbohydrates, amino acids and soluble proteins, which play a role in osmotic adjustment were estimated to investigate the role of potassium nitrate and ammonium sulphate as osmoregulators and their effects on the solutes composition. Vicia faba L. was cultivated and irrigated with 5, 10, 15 and 20 % (v:v) sea water. The plants were divided to three groups. The first was irrigated with sea water only. The second was treated with 5 mM KNO3 while the third was treated with 5mM (NH4)2SO4. The plants were left to grow until flowering stage. The results indicated that the non treated group increased the soluble carbohydrates in the roots to avoid the influx of sodium. The treatment with... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Osmoregulation; Irrigation; Salinity; Sea water; Vicia faba. |
Ano: 2012 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000200006 |
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Rosso,Felipe Link de; Bolner,Keidi C. S.; Baldisserotto,Bernardo. |
Low dissolved oxygen levels in the water (hypoxia) can be provoked by oxygen consumption by fish and other organisms, organic matter decomposition, phytoplankton blooms, and temperature increase. The objective of the present study was to investigate Na+, Cl-, K+, and ammonia fluxes in silver catfish (Rhamdia quelen) exposed to different dissolved oxygen levels. Juveniles (9 ± 1g) maintained at 6.0 mg.L-1 dissolved oxygen were transferred to four 40 L aquaria with different dissolved oxygen levels (in mg.L-1): 6.0, 4.5, 3.5, and 2.5. In another series of experiments, juveniles were acclimated at 6.0 or 2.5 mg.L-1 dissolved oxygen levels, and then placed in two 40 L aquaria with 6.0 mg.L-1 dissolved oxygen. For both series of experiments, 1, 24, 48 or 120 h... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oxygen saturation; Osmoregulation; Jundiá. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252006000400007 |
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Blondeau-bidet, Eva; Hiroi, Junya; Lorin-nebel, Catherine. |
Ion uptake mechanisms are diverse in fish species, certainly linked to duplication events that have led to the presence of a multitude of paralogous genes. In fish, Na+ uptake involves several ion transporters expressed in different ionocyte subtypes. In the European sea bass Dicentrarchus labrax, several key transporters potentially involved in Na+ uptake have been investigated in seawater (SW) and following a 2 weeks freshwater (FW) acclimation. Using gel electrophoresis, we have shown that the Na+/H+-exchanger 3 (nhe3, slc9a3) is expressed in gills and kidney at both salinities. Quantitative realtime PCR analysis showed a significantly higher nhe3 expression in fresh water (FW) compared to SW. Its apical localization in a subset of gill ionocytes in... |
Tipo: Text |
Palavras-chave: Teleost; Osmoregulation; Ion transporters; Na+ uptake; NHE3; NCC2. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00485/59618/62638.pdf |
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Registros recuperados: 45 | |
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