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	Provedor de dados BJMBR (2) BJID (1) Autor Alexandrino,A.M. (1) Chuery,Ana Carolina Silva (1) Laudanna,A.A. (1) Mattar,R. (1) Monteiro,E. (1) Moura,R.P. (1) Pinheiro,N.A. (1) Ribalta,Julisa Chamorro Lascasas (1) Silva,Ismael Dale Cotrim Guerreiro da (1) Speck,Neila Maria de Góis (1) Villa,L.L. (1) Mais... Palavra-chave P53 gene (3) Human papillomavirus (2) Arginine (1) Cancer (1) Genetic polymorphisms (1) LOH (1) Mutation (1) Oncoproteins (1) PCR (1) SSCP (1) Silver staining (1) Ulcerative colitis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Info:eu-repo/semantics/other (1) Ano 2017 (1) 1999 (2) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Association between the p53 arginine/arginine homozygous genotype at codon 72 and human papillomavirus E6/E7 mRNA expression BJID Chuery,Ana Carolina Silva; Silva,Ismael Dale Cotrim Guerreiro da; Ribalta,Julisa Chamorro Lascasas; Speck,Neila Maria de Góis. ABSTRACT Objective: To evaluate the association between p53 polymorphisms and human papillomavirus (HPV) E6/E7 mRNA expression. Methods: We analyzed 175 cervical samples from women aged 16-69 years old who were tested for HPV E6/E7 mRNA expression (NucliSENS® EasyQ® HPV). The samples were divided into three groups: positive (n = 75) those with positive HPV E6/E7 mRNA expression and positive high-risk HPV Hybrid Capture (HR-HC) test; negative (n = 52) those with negative HPV E6/E7 mRNA expression and positive HR-HC; and control (n = 48) those with negative HPV E6/E7 mRNA expression and negative HR-HC. The p53 polymorphisms at codons 11, 72, and 248 were evaluated through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).... Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Oncoproteins; Genetic polymorphisms; P53 gene; Arginine. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300248 Detection of point mutations by non-isotopic single strand conformation polymorphism BJMBR Pinheiro,N.A.; Moura,R.P.; Monteiro,E.; Villa,L.L.. We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53. The protocol does not require any particular facilities or equipment, such as radioactive handling, large gel units for sequencing, or a semiautomated electrophoresis system. This technique consists of amplification of DNA fragments by PCR with specific oligonucleotide primers, denaturation, and electrophoresis on small neutral polyacrylamide gels, followed by silver staining. The sensitivity of this procedure is comparable to other described techniques and the method is easy to perform and applicable to a variety of tissue specimens. Tipo: Info:eu-repo/semantics/other Palavras-chave: P53 gene; Human papillomavirus; PCR; SSCP; Cancer; Silver staining. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100008 Infrequent p53 gene alterations in ulcerative colitis BJMBR Mattar,R.; Alexandrino,A.M.; Laudanna,A.A.. The purpose of this study was to determine whether point mutations and loss of the p53 gene take place in ulcerative colitis which is histologically negative for dysplasia. DNA was extracted from 13 frozen rectal or colon biopsies and blood samples. Ulcerative colitis was classified histologically as active (10 cases) and inactive (3 cases). Exons 5-8 were amplified by PCR, treated with exonuclease and shrimp alkaline phosphatase and sequenced by the dideoxy chain termination method with the Sequenase Version 2.0 DNA sequencing kit. PCR products of intron 6 and exon 4 were digested with MspI and AccII, respectively, for RFLP analysis. No p53 gene mutation was detected in these cases. The number of informative patients for loss of heterozygosity (LOH) at... Tipo: Info:eu-repo/semantics/article Palavras-chave: P53 gene; Ulcerative colitis; LOH; Mutation. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900005 Registros recuperados: 3 Primeira ... 1 ... Última

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