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| Registros recuperados: 198 | |
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| Velge, P.; Herler, M.; Johansson, J.; Roche, S.; Témoin, S.; Fedorov, A. A.; Gracieux, P.; Almo, S. C.; Goebel, W.; Cossart, P.. |
| The sequencing of prfA, encoding the transcriptional regulator of virulence genes, in 26 low virulence field Listeria monocytogenes strains showed that eight strains exhibited the same single amino-acid substitution: PrfAK220T. These strains exhibited no expression of PrfA-regulated proteins and thus no virulence. This substitution inactivated PrfA, since expression of the PrfAK220T mutant gene in an EGDΔprfA strain did not restore the haemolytic and phosphatidylcholine phospholipase C activities, in contrast to the wild-type prfA gene. The substitution of the lysine at position 220 occurred in the helix αH. However, the data showed that the PrfAK220T protein is dimerized just as well as its wild-type counterpart, but does not bind to PrfA boxes.... |
| Tipo: Journal Article |
Palavras-chave: LISTERIA MONOCYTOGENES; PROTEINE; FACTEUR DE VIRULENCE; BACTERIE PATHOGENE; ADN; SEQUENCE NUCLEOTIDIQUE; REGULATION; TRANSCRIPTION; VIRULENCE; EXPRESSION DES GENES; ACIDE AMINE; MUTATION; RT-PCR; ELECTROPHORESE; ENZYME; ARN POLYMERASE. |
| Ano: 2007 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009c1895b3c&uri=/notices/prodinra1/2009/11/ |
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| Monsarrat, Annie; Abol-Ela, S.; Abdel-Hamid, I.; Fédière, Gilles; Kuhl, G.; El Husseini, M.; Giannotti, J.. |
| Un nouveau virus infectant le ver rose du cotonnier, #Pectinophora gossypiella$, a été isolé et purifié à partir de larves mortes récoltées en champ de coton. Les virions purifiés de 27 plus ou moins 1 nm de diamètre, ont une forme icosaédrique et contiennent un génome à ARN. Trois protéines capsidaires de 31,7, 32,6 et 47,4 Kd ont été trouvées dans les virions. Le virus purifié n'est pas hautement infectieux pour les larves, cependant la durée du stade chrysalide est supérieure chez les lots infectés artificiellement. Dans les tissus atteints, les virions sont dispersés dans le cytoplasme des cellules de l'intestin ou bien groupés en amas paracristallins de même type que chez d'autres insectes infectés avec des picornavirus. Les adultes malades... |
| Tipo: Text |
Palavras-chave: INSECTE NUISIBLE; COTON; VIRUS; PURIFICATION; ARN; PROTEINE; ULTRASTRUCTURE; POUVOIR PATHOGENE; PARASITOIDE. |
| Ano: 1995 |
URL: http://www.documentation.ird.fr/hor/fdi:010006113 |
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| Le François, Patrice; Chevassus-Agnès, Simon; Diao, S. (collab.). |
| Une enquête de consommation alimentaire par pesée a été faite pendant 3 jours chez 38 nourrissons sénégalais afin d'apprécier la part de l'alimentation complémentaire dans la couverture de leurs besoins nutritionnels. Les apports recommandés en énergie et en protéines sont satisfaits respectivement à 49 et à 74%. Les besoins nutritionnels sont couverts à des niveaux variables : calcium 38%, fer 24%, vitamine A 39%, B1 51%, B2 43%, PP 82%, C 54%, folates 8%, B12 263%. En tenant compte de l'estimation des apports assurés par le lait maternel, les apports recommandés en énergie et en protéines sont satisfaits pendant la période étudiée. (Résumé d'auteur) |
| Tipo: Text |
Palavras-chave: ENQUETE ALIMENTAIRE; COMPLEMENTATION; ALLAITEMENT MATERNEL; NOURRISSON; BESOIN NUTRITIONNEL; APPORT NUTRITIONNEL; APPORT RECOMMANDE; PROTEINE; VALEUR ENERGETIQUE. |
| Ano: 1983 |
URL: http://www.documentation.ird.fr/hor/fdi:03997 |
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| Aubourg, S.; Martin-Magniette, M.L.; Brunaud, V.; Taconnat, L.; Bitton, F.; Balzergue, S.; Jullien, P.E.; Ingouff, M.; Thareau, V.; Schiex, T.; Lecharny, A.; Renou, J.P.. |
| Background: Since the finishing of the sequencing of the Arabidopsis thaliana genome, the Arabidopsis community and the annotator centers have been working on the improvement of gene annotation at the structural and functional levels. In this context, we have used the large CATMA resource on the Arabidopsis transcriptome to search for genes missed by different annotation processes. Probes on the CATMA microarrays are specific gene sequence tags (GSTs) based on the CDS models predicted by the Eugene software. Among the 24 576 CATMA v2 GSTs, 677 are in regions considered as intergenic by the TAIR annotation. We analyzed the cognate transcriptome data in the CATMA resource and carried out data-mining to characterize novel genes and improve gene models.... |
| Tipo: Journal Article |
Palavras-chave: ADNC; EXPRESSION DES GENES; SEQUENCE NUCLEOTIDIQUE; ARABIDOPSIS THALIANA; GENOME; MODELE; METHODE STATISTIQUE; PROTEINE; TRANSCRIPTION; ARN MESSAGER; PCR; RT-PCR CDNA MICROARRAY DATA; EXPRESSION PROFILES; SEQUENCE TAGS; ANNOTATION; THALIANA; NORMALIZATION; REGIONS; TOOLS. |
| Ano: 2007 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20116115e9a4&uri=/notices/prodinra1/2011/03/ |
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| Chertemps, T.; Mitri, C.; Perrot, S.; Sautereau, J.; Jacques, J.-C.; Thiery, I.; Bourgouin, C.; Rosinski-Chupin, I.. |
| Background: Invasion of the mosquito salivary glands by Plasmodium is a critical step for malaria transmission. From a SAGE analysis, we previously identified several genes whose expression in salivary glands was regulated coincident with sporozoite invasion of salivary glands. To get insights into the consequences of these salivary gland responses, here we have studied one of the genes, PRS1 (Plasmodium responsive salivary 1), whose expression was upregulated in infected glands, using immunolocalization and functional inactivation approaches. Methodology/Principal Findings: PRS1 belongs to a novel insect superfamily of genes encoding proteins with DM9 repeat motifs of uncharacterized function. We show that PRS1 is induced in response to Plasmodium, not... |
| Tipo: Journal Article |
Palavras-chave: PLASMODIUM; MOUSTIQUE; GLANDE SALIVAIRE; SALIVE; MALADIE INFECTIEUSE; PROTEINE; TRANSMISSION DE LA MALADIE; VECTEUR DE LA MALADIE; INTERACTION MOLECULAIRE; RT-PCR; SDS-PAGE; ARTHROPODA; HEXAPODA; EXPRESSION DES GENES; MICROSCOPIE CONFOCALE; INSECTE; PARASITOSE MALARIA PARASITE; MOLECULAR-INTERACTIONS; BERGHEI SPOROZOITES; MOSQUITO; INVASION; INFECTION; PROTEIN; VECTOR; CELLS; FALCIPARUM. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010f5c08798&uri=/notices/prodinra1/2010/11/ |
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| Thomas, A. W.; Narum, D.; Waters, A. P.; Trape, Jean-François; Rogier, C.; Gonçalves, A.; Rosario, V.; Druilhe, P.; Mitchell, G. H.; Dennis, D.. |
| Tipo: Text |
Palavras-chave: PALUDISME; AGENT PATHOGENE; PARASITE; ANTIGENE; PROTEINE; IMMUNITE; SEROLOGIE; VACCINATION; ELISA. |
| Ano: 1994 |
URL: http://www.documentation.ird.fr/hor/fdi:43280 |
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| Torréton, Jean-Pascal; Dufour, Philippe. |
| This study compares three independent methods used for estimating bacterioplankton production in waters from the lagoon (mesotrophic) and the surrounding ocean (oligotrophic) of two atolls from the Tuamotu archipelago (French Polynesia). Thymidine and leucine incorporation were calibrated in dilution cultures and gave consistent results when the first was calibrated against cell multiplication and the second against protein synthesis. This study demonstrates that determining conversion factors strongly depends on the selected calculation method (modified derivative, integrative, and cumulative). These different estimates are reconciled when the very low proportion of active cells is accounted for. Frequency of dividing-divided cells (FDDC) calibrated using... |
| Tipo: Text |
Palavras-chave: MICROPLANCTON; BACTERIE; LAGON; OCEAN; ABONDANCE; BIOMASSE; ACIDE AMINE; PROTEINE; ETUDE COMPARATIVE. |
| Ano: 1996 |
URL: http://www.documentation.ird.fr/hor/fdi:010013312 |
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| Registros recuperados: 198 | |
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