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	Provedor de dados BJMBR (3) OAK (1) Biocell (1) Autor Beirão,P.S.L. (1) Bustamante,J.O. (1) Costa,K.M. (1) Cruz,J.S. (1) De Rosas,Juan C. (1) Fóscolo,Mabel (1) Ibáñez,Jorge E. (1) Ichii, Osamu (1) Ishikawa, Toru (1) Ituarte,Leonor M.E. (1) Kamikawa, Akihiro (1) Leão,R.M. (1) Mafra,R.A. (1) Sakazaki, Junpei (1) Saldeña,Teobaldo A. (1) Santos,D.V.V. (1) Saraví,Fernando D. (1) Silva Filho,M. Da (1) Varanda,W.A. (1) Vaz,M.C.G. (1) Mais... Palavra-chave Patch-clamp (5) 4-Aminopyridine (1) Biocytin (1) CaCC (1) Cell nucleus (1) Cerebellar granule neurons (1) Dendritic morphology (1) Excitatory amino-acid transporter type 2 (1) Fertilization (1) Gene activity (1) Gene expression (1) Glutamate (1) Hamster oocyte (1) Ion channels (1) Lactation (1) Layer 1 (1) Macromolecular transport (1) Mammary gland (1) Mammary secretory cell (1) Measurement (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2016 (1) 2012 (1) 2006 (1) 2003 (1) 1998 (1) País Brazil (3) Argentina (1) Japan (1) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Ca2+-activated Cl- channel currents in mammary secretory cells from lactating mouse OAK Kamikawa, Akihiro; Ichii, Osamu; Sakazaki, Junpei; Ishikawa, Toru. The Cl- secretion via Ca2+-activated Cl- channel (CaCC) is critical for fluid secretion in exocrine glands like the salivary gland. Also in the mammary gland, it has been hypothesized that CaCC plays an important role in the secretion of Cl- and aqueous phase of milk. However, there has been no evidence for the functional expression of CaCC in native mammary secretory (MS) cells of lactating animals. We therefore assessed membrane current in MS cells that were freshly isolated from lactating mice using whole cell patch-clamp techniques. In MS cells, we detected CaCC current that exhibited the following characteristics: 1) Ca2+-dependent activation at the concentrations of sub-micromolar range; 2) voltage-dependent activation; 3) slow kinetics for... Palavras-chave: CaCC; Lactation; Mammary gland; Mammary secretory cell; Patch-clamp. Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4553 Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons BJMBR Mafra,R.A.; Leão,R.M.; Beirão,P.S.L.; Cruz,J.S.. A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 µM and a maximum increase of 51.2 ± 4.4%. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerebellar granule neurons; Excitatory amino-acid transporter type 2; Patch-clamp; Glutamate; Whole-cell voltage-clamp. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700018 Patch-clamp detection of macromolecular translocation along nuclear pores BJMBR Bustamante,J.O.; Varanda,W.A.. The present paper reviews the application of patch-clamp principles to the detection and measurement of macromolecular translocation along the nuclear pores. We demonstrate that the tight-seal 'gigaseal' between the pipette tip and the nuclear membrane is possible in the presence of fully operational nuclear pores. We show that the ability to form a gigaseal in nucleus-attached configurations does not mean that only the activity of channels from the outer membrane of the nuclear envelope can be detected. Instead, we show that, in the presence of fully operational nuclear pores, it is likely that the large-conductance ion channel activity recorded derives from the nuclear pores. We conclude the technical section with the suggestion that the best way to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nuclear pores; Nuclear envelope; Cell nucleus; Nucleocytoplasmic transport; Macromolecular transport; Gene activity; Gene expression; Transcriptional control; Ion channels; Patch-clamp; Measurement. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300003 Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons BJMBR Santos,D.V.V.; Costa,K.M.; Vaz,M.C.G.; Silva Filho,M. Da. The cortical layer 1 contains mainly small interneurons, which have traditionally been classified according to their axonal morphology. The dendritic morphology of these cells, however, has received little attention and remains ill defined. Very little is known about how the dendritic morphology and spatial distribution of these cells may relate to functional neuronal properties. We used biocytin labeling and whole cell patch clamp recordings, associated with digital reconstruction and quantitative morphological analysis, to assess correlations between dendritic morphology, spatial distribution and membrane properties of rat layer 1 neurons. A total of 106 cells were recorded, labeled and subjected to morphological analysis. Based on the quantitative... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocytin; Patch-clamp; Dendritic morphology; Layer 1; Neuronal variability. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200016 Single-channel response of hamster oocytes to ertilization with homologous spermatozoa Biocell Ituarte,Leonor M.E.; Viera,Teresa B.; Saldeña,Teobaldo A.; De Rosas,Juan C.; Fóscolo,Mabel; Ibáñez,Jorge E.; Saraví,Fernando D.. Electrophysiological events occur early after fertilization, along with changes in intracellular Ca2+ concentration . Passive electrical parameters were determined in golden hamster oocytes by whole cell patch-clamp method. In separate experiments the effect of 4-aminopyridine on resting oocytes was tested. The single-channel patch clamp configuration was employed to assess the electrical response to fertilization with homologous sperm. Structure of oocytes submitted to patch clamp was evaluated with scanning electron microscopy and found to be preserved. Oocyte diameter was 70.2 ±  2.2 µm; their resting parameters were: membrane potential 23.8 ±  0.8 mV; total membrane specific resistance 519.1 ±  94.6 Ω.cm², and specific capacity 0.99 ±  0.03... Tipo: Info:eu-repo/semantics/article Palavras-chave: 4-Aminopyridine; Fertilization; Hamster oocyte; Patch-clamp; Membrane current. Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100007 Registros recuperados: 5 Primeira ... 1 ... Última

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