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Phenotypic and genotypic characterization of Salmonella Enteritidis isolates 58
Oliveira,Sílvia Dias de; Bessa,Marjo Cadó; Santos,Luciana Ruschel dos; Cardoso,Marisa Ribeiro de Itapema; Brandelli,Adriano; Canal,Cláudio Wageck.
In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing were also associated to rep-PCR results. One hundred and two S. Enteritidis isolates from broiler carcasses, food, human, pigs, poultry-related samples, and nine isolates from other countries were genotypically typed by REP-PCR, ERIC-PCR and BOX-PCR, collectively called rep-PCR. Phage typing, detection of virulence genes and antimicrobial resistance testing were also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella Enteritidis; Rep-PCR; Phage typing; Antimicrobial resistance; Virulence genes.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400025
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Salmonella spp. in raw broiler parts: occurrence, antimicrobial resistance profile and phage typing of the Salmonella Enteritidis isolates 58
Ribeiro,Aldemir Reginato; Kellermann,Aline; Santos,Luciana Ruschel dos; Bessa,Marjo Cadó; Nascimento,Vladimir Pinheiro do.
The present study was carried out to evaluate the occurrence of Salmonellae in raw broiler parts and to determine the antimicrobial resistance profile of the isolated strains. Twenty-four (39.3%) broiler parts samples were positive for Salmonella and twenty-five Salmonella strains were isolated, since two different serovars were detected in one single positive sample. Salmonella Enteritidis was the most prevalent serovar. Among Salmonella Enteritidis isolates, 95.2% belonged to Phage Type 4 (PT4) (20/21) and 4.8% to PT7 (1/21). Twenty-two (88%) strains of Salmonella were resistant to at least one antimicrobial agent, generating eight different resistance patterns. The S. Typhimurium (n: 1) and S. Hadar (n: 3) isolates presented multiple resistance. Three...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Broiler parts; Antimicrobial resistance; Phage typing.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200021
Registros recuperados: 2
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