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Registros recuperados: 7
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Activity of enzymes associated with the enzymatic browning of minimally processed potatoes BABT
Vitti,Maria Carolina Dario; Sasaki,Fabiana Fumi; Miguel,Patrícia; Kluge,Ricardo Alfredo; Moretti,Celso Luiz.
The purpose of the present study was to evaluate the effect of different potato cultivars and storage temperatures on the specific activity of phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) in minimally processed potatoes. Potato cultivars Agata, Asterix and Monalisa were selected, washed, peeled, diced, sanitized, centrifuged, vacuum- packed and stored at 5 and 15°C for 9 and 5 days, respectively. There was an increase in the enzymatic activity in all the cultivars stored at 15°C. The cultivars 'Agata' and 'Asterix' stored at 5ºC did not differ significantly between them for the PAL, PPO and POD activities. The PAL, PPO and POD activities were also influenced by the storage temperature. The cultivars Agata and Asterix...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum tuberosum L; Phenylalanine ammonia-lyase; Polyphenol oxidase; Peroxidase; Minimal processing.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500016
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cDNA cloning, Phylogenic Analysis and Gene Expression Pattern of Phenylalanine ammonia-lyase in Sugarcane (Saccharum officinarum L.) BABT
Hashemitabar,Mahmoud; Kolahi,Maryam; Tabandeh,Mohammad Reza; Jonoubi,Parisa; Majd,Ahmad.
The aim of the present study was to clone and characterize a full length cDNA of sugarcane (Saccharum officinarum) phenylalanine ammonia-lyase (SoPAL). Differential tissue expression pattern of the SoPAL transcript and its enzyme activity was also analyzed during the tillering stage of growth. The full-length of SoPAL cDNA was 2118 bp long and contained a protein with 706 amino acids, determined by encoding technique. The amino acid sequence and phylogenic analysis of the cloned SoPAL showed high similarity to PAL from other monocotyledonous such as sorghum (96%), maize (93%) and Bamboos (87.12%). The highest levels of SoPAL transcript were observed in the root and stem, while its minimal gene expression levels were in the leaves and sheath, respectively....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenylalanine ammonia-lyase; Saccharum officinarumL; Cloning; Tissue expression.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400456
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Chitosan derivatives induce local and distal expression of defence-related genes in wheat (Triticum aestivum L.) seedlings Agrociencia
Díaz-Martínez,Juan M.; Aispuro-Hernández,Emmanuel; Vargas-Arispuro,Irasema; Falcón-Rodríguez,Alejandro B.; Martínez-Téllez,Miguel Á..
Abstract Chitosan and its derivatives are elicitors of defence responses in plants. The physical and chemical characteristics, like the molecular weight and acetylation degree determine the type of effect and specificity. Although chitosan-induced biochemical and enzymatic reactions in wheat (Triticum aestivum) are reported, the transcriptional activation of defensive markers by chitosan derivatives remains unaddressed. The hypothesis of our study was that the different structural features of chitosan derivatives would influence the transcriptional patterns related to protective responses in wheat. The aim of our study was to compare the local and systemic inductive properties of chitosan and chitooligosaccharides (COS) in wheat seedlings through the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Chitooligosaccharide; Glucanase; Phenylalanine ammonia-lyase; Systemic resistance.
Ano: 2018 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952018000400497
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Genotypic differences in cyanogenic glycosides levels of compatible Prunus persica P. persica and incompatible P. persica P. mume combinations Bragantia
Pereira,Ivan dos Santos; Pina,Ana; Antunes,Luis Eduardo Correa; Campos,Ângela Diniz; Fachinello,José Carlos.
ABSTRACT Graft incompatibility is a phenomenon associated with complex physiological, biochemical, and genetic interactions between scion and rootstock. The main objective of this work was to assess the role of cyanogenic glycosides (CGs), amygdalin and prunasin, in the graft incompatibility of Prunus and possible biochemical effects in compounds of the phenylpropanoid pathway. Graft compatibility, amygdalin and prunasin content, phenylalanine ammonia-lyase activity, total phenolic compounds content and antioxidant activity, were studied in different graft combinations (Chimarrita/Capdeboscq; Chimarrita/Tsukuba 1; Chimarrita/Umezeiro; Maciel/Capdeboscq; Maciel/’Tsukuba 1; Maciel/Umezeiro) and ungrafted genotypes. The results indicate that there was graft...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Graft incompatibility; Amygdalin; Prunasin; Phenylalanine ammonia-lyase; Oxidative stress.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000100001
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Naringenin inhibits the growth and stimulates the lignification of soybean root BABT
Bido,Graciene de Souza; Ferrarese,Maria de Lourdes Lucio; Marchiosi,Rogério; Ferrarese-Filho,Osvaldo.
The flavanone naringenin, an intermediate in flavonoid biosynthesis, was tested for its effect on root growth, phenylalanine ammonia-lyase (PAL) and peroxidase (POD) activities, as well as phenolic compounds and lignin contents in soybean (Glycine max L. Merrill) seedlings. Three-day-old seedlings were cultivated in half-strength Hoagland nutrient solution (pH 6.0), with or without 0.1 to 0.4 mM naringenin in a growth chamber (25°C, 12-h photoperiod, irradiance of 280 µmol m-2 s-1) for 24 h. Inhibitory effects on root growth (length, weight, cell viability), PAL and soluble POD activities were detected after naringenin treatments. These effects were associated with stimulatory activity of the cell wall-bound POD followed by an increase in the lignin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allelopathy; Lignin; Flavonoid; Peroxidase; Phenylalanine ammonia-lyase; Root growth.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300005
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Peroxidase and phenylalanine ammonia-lyase activities, phenolic acid contents, and allelochemicals-inhibited root growth of soybean Biol. Res.
HERRIG,VANESSA; FERRARESE,MARIA DE LOURDES L.; SUZUKI,LETÍCIA S.; RODRIGUES,JOÃO DOMINGOS; FERRARESE-FILHO,OSVALDO.
The influence of the allelochemicals ferulic (FA) and vanillic (VA) acids on peroxidase (POD, EC 1.11.1.7) and phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activities and their relationships with phenolic acid (PhAs) contents and root growth of soybean (Glycine max (L.) Merr.) were examined. Three-day-old seedlings were cultivated in nutrient solution containing FA or VA (0.1 to 1 mM) for 48 h. Both compounds (at 0.5 and 1 mM) decreased root length (RL), fresh weight (FW) and dry weight (DW) and increased PhAs contents. At 0.5 and 1 mM, FA increased soluble POD activity (18% and 47%, respectively) and cell wall (CW)-bound POD activity (61% and 34%), while VA increased soluble POD activity (33% and 17%) but did not affect CW-bound POD activity. At 1 mM, FA...
Tipo: Journal article Palavras-chave: Roots; Phenolic acids; Phenylalanine ammonia-lyase; Peroxidase; Soybean.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100009
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アルファルファとVerticillium albo-atrumの相互作用 : 6. 菌体細胞壁成分に対して高いPAL活性を示す細胞系統の選抜とその特性 OAK
Koike, Masanori; Katsumata, Yukiyoshi; Amemiya, Yoshimiki; Shimada, Tohru.
To obtain alfalfa cell lines with high phenylalanine ammonia-lyase (PAL) activity in response to heat-released cell wall components (elicitor) of an alfalfa pathogen (Verticillium albo-atrum), in vitro selection was performed. The selection using a replica plating methods was carried out on 65 cell lines derived from suspension cultures treated and/or non-treated with N-metyl-N'-nitro-N-nitrosoguanidine (MNNG) of the susceptible genotype of cv. Vertus. Three stable high PAL cell lines in response to elicitor (two cell lines recovered from MNNG treatment, one from non-MNNG treatment) were isolated. All these cell lines showed higher PAL activities, lignin depositions and phytoalexin accumulations than the parental cell line after treatment with active V....
Palavras-chave: Alfalfa; In vitro selection; Phenylalanine ammonia-lyase; アルファルファ; 細胞選抜; Verticillium albo-atrum; フェニルアラニンアンモニアリアーゼ.
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1770
Registros recuperados: 7
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