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Registros recuperados: 10 | |
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Kato, Kentaro; Sugi, Tatsuki; Takemae, Hitoshi; Takano, Ryo; Gong, Haiyan; Ishiwa, Akiko; Horimoto, Taisuke; Akashi, Hiroomi. |
Background: Toxoplasma gondii is an obligate intracellular parasite of the phylum Apicomplexa and a major pathogen of animals and immunocompromised humans, in whom it causes encephalitis. Understanding the mechanism of tachyzoite invasion is important for the discovery of new drug targets and may serve as a model for the study of other apicomplexan parasites. We previously showed that Plasmodium falciparum expresses a homolog of human calcium calmodulin-dependent protein kinase (CaMK) that is important for host cell invasion. In this study, to identify novel targets for the treatment of Toxoplasma gondii infection (another apicomplexan parasite), we sought to identify a CaMK-like protein in the T. gondii genome and to characterize its role in the... |
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Palavras-chave: Calcium calmodulin-dependent protein kinase homolog; GAP45; Phosphorylation; T. gondii CaMK-related kinase; Toxoplasma gondii. |
Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4447 |
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Song,G.; Mao,Y.B.; Cai,Q.F.; Yao,L.M.; Ouyang,G.L.; Bao,S.D.. |
Curcumin, a major yellow pigment and active component of turmeric, has multiple anti-cancer properties. However, its molecular targets and mechanisms of action on human colon adenocarcinoma cells are unknown. In the present study, we examined the effects of curcumin on the proliferation of human colon adenocarcinoma HT-29 cells by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide method and confirmed the curcumin-induced apoptosis by morphology and DNA ladder formation. At the same time, p53, phospho-p53 (Ser15), and other apoptosis-related proteins such as Bax, Bcl-2, Bcl-xL, pro-caspase-3, and pro-caspase-9 were determined by Western blot analysis. The colon adenocarcinoma cells were treated with curcumin (0-75 µM) for 0-24 h. We observed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Curcumin; Apoptosis; HT-29 cells; P53; Phosphorylation; DNA ladder. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200007 |
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Hei,Ming-Yan; Tao,Hui-Kang; Tang,Qin; Yu,Bo; Zhao,Ling-Ling. |
Our objective was to investigate the protein level of phosphorylated N-methyl-D-aspartate (NMDA) receptor-1 at serine 897 (pNR1 S897) in both NMDA-induced brain damage and hypoxic-ischemic brain damage (HIBD), and to obtain further evidence that HIBD in the cortex is related to NMDA toxicity due to a change of the pNR1 S897 protein level. At postnatal day 7, male and female Sprague Dawley rats (13.12 ± 0.34 g) were randomly divided into normal control, phosphate-buffered saline (PBS) cerebral microinjection, HIBD, and NMDA cerebral microinjection groups. Immunofluorescence and Western blot (N = 10 rats per group) were used to examine the protein level of pNR1 S897. Immunofluorescence showed that control and PBS groups exhibited significant neuronal... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: N-methyl-D-aspartate receptor 1; Phosphorylation; Neonate; Hypoxic-ischemic brain damage; Cerebral microinjection; Brain damage. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000012 |
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PATTERSON,SEAN I. |
Protein domains play a fundamental role in the spatial and temporal organization of intracellular signaling systems. While protein phosphorylation has long been known to modify the interactions that underlie this organization, the dynamic cycling of lipids should now be included amongst the posttranslational processes determining specificity in signal transduction. The characteristics of this process are reminiscent of the properties of protein and lipid phosphorylation in determining compartmentalization through SH2 or PH domains. Recent studies have confirmed the functional importance of protein S-palmitoylation in the compartmentalization of signaling molecules that support normal physiological function in cell division and apoptosis, and synaptic... |
Tipo: Journal article |
Palavras-chave: S-palmitoylation; Synapse; Growth cone; Regeneration; Phosphorylation. |
Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200005 |
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Registros recuperados: 10 | |
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