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Registros recuperados: 11
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Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homolog OAK
Kato, Kentaro; Sugi, Tatsuki; Takemae, Hitoshi; Takano, Ryo; Gong, Haiyan; Ishiwa, Akiko; Horimoto, Taisuke; Akashi, Hiroomi.
Background: Toxoplasma gondii is an obligate intracellular parasite of the phylum Apicomplexa and a major pathogen of animals and immunocompromised humans, in whom it causes encephalitis. Understanding the mechanism of tachyzoite invasion is important for the discovery of new drug targets and may serve as a model for the study of other apicomplexan parasites. We previously showed that Plasmodium falciparum expresses a homolog of human calcium calmodulin-dependent protein kinase (CaMK) that is important for host cell invasion. In this study, to identify novel targets for the treatment of Toxoplasma gondii infection (another apicomplexan parasite), we sought to identify a CaMK-like protein in the T. gondii genome and to characterize its role in the...
Palavras-chave: Calcium calmodulin-dependent protein kinase homolog; GAP45; Phosphorylation; T. gondii CaMK-related kinase; Toxoplasma gondii.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4447
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Collateral methotrexate resistance in cisplatin-selected murine leukemia cells BJMBR
Bhushan,A.; Hacker,M.P.; Tritton,T.R..
Resistance to anticancer drugs is a major cause of failure of many therapeutic protocols. A variety of mechanisms have been proposed to explain this phenomenon. The exact mechanism depends upon the drug of interest as well as the tumor type treated. While studying a cell line selected for its resistance to cisplatin we noted that the cells expressed a >25,000-fold collateral resistance to methotrexate. Given the magnitude of this resistance we elected to investigate this intriguing collateral resistance. From a series of investigations we have identified an alteration in a membrane protein of the resistant cell as compared to the sensitive cells that could be the primary mechanism of resistance. Our studies reviewed here indicate decreased tyrosine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Methotrexate transport; Resistance; Cisplatin; Leukemia; Phosphorylation.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700005
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Curcumin induces human HT-29 colon adenocarcinoma cell apoptosis by activating p53 and regulating apoptosis-related protein expression BJMBR
Song,G.; Mao,Y.B.; Cai,Q.F.; Yao,L.M.; Ouyang,G.L.; Bao,S.D..
Curcumin, a major yellow pigment and active component of turmeric, has multiple anti-cancer properties. However, its molecular targets and mechanisms of action on human colon adenocarcinoma cells are unknown. In the present study, we examined the effects of curcumin on the proliferation of human colon adenocarcinoma HT-29 cells by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide method and confirmed the curcumin-induced apoptosis by morphology and DNA ladder formation. At the same time, p53, phospho-p53 (Ser15), and other apoptosis-related proteins such as Bax, Bcl-2, Bcl-xL, pro-caspase-3, and pro-caspase-9 were determined by Western blot analysis. The colon adenocarcinoma cells were treated with curcumin (0-75 µM) for 0-24 h. We observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Curcumin; Apoptosis; HT-29 cells; P53; Phosphorylation; DNA ladder.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200007
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Decreased levels of pNR1 S897 protein in the cortex of neonatal Sprague Dawley rats with hypoxic-ischemic or NMDA-induced brain damage BJMBR
Hei,Ming-Yan; Tao,Hui-Kang; Tang,Qin; Yu,Bo; Zhao,Ling-Ling.
Our objective was to investigate the protein level of phosphorylated N-methyl-D-aspartate (NMDA) receptor-1 at serine 897 (pNR1 S897) in both NMDA-induced brain damage and hypoxic-ischemic brain damage (HIBD), and to obtain further evidence that HIBD in the cortex is related to NMDA toxicity due to a change of the pNR1 S897 protein level. At postnatal day 7, male and female Sprague Dawley rats (13.12 ± 0.34 g) were randomly divided into normal control, phosphate-buffered saline (PBS) cerebral microinjection, HIBD, and NMDA cerebral microinjection groups. Immunofluorescence and Western blot (N = 10 rats per group) were used to examine the protein level of pNR1 S897. Immunofluorescence showed that control and PBS groups exhibited significant neuronal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: N-methyl-D-aspartate receptor 1; Phosphorylation; Neonate; Hypoxic-ischemic brain damage; Cerebral microinjection; Brain damage.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000012
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Effect of Phosphorylation and Copper(II) or Iron(II) Ions Enrichment on Some Physicochemical Properties of Spelt Starch BABT
Rożnowski,Jacek; Fortuna,Teresa; Nowak,Katarzyna; Szuba,Edyta.
ABSTRACT: This paper provides an assessment of the effect of saturation of spelt starch and monostarch phosphate with copper or iron ions on selected physicochemical properties of the resulting modified starches. Native and modified spelt starch samples were analyzed for selected mineral element content using Atomic Absorption Spectroscopy (AAS). Thermodynamic properties were measured using DSC, and pasting properties by RVA. Flow curves of 5% pastes were plotted and described using the Herschel-Bulkley model. The structure recovery ratio was measured. AAS analysis established the presence of iron(II) and copper(II) ions in the samples of modified starches and that potassium and magnesium ions had leached from them. In comparison to unfortified samples,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chemically modified starch; Enrichment; Phosphorylation; Spelt starch.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100511
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Etude d'oligosaccharides bioactifs issus d'exopolysaccharides bactériens : obtention, caractérisation et relation structure/fonction ArchiMer
Roger, Olivier.
Unusual exopolysaccharides (EPS) produced by heterotrophic aerobic and mesophilic bacteria originating from hydrothermal vent, were reviewed as a new source of polysaccharidic structures endowed with innovative biological properties. The first step of this study was the structure determination of the EPS GY785, synthesized by Alteromonas infernus. A highly branched nonasaccharidic repetitive unit was identified using chemical modifications and nuclear magnetic resonance spectroscopy. This 106 g/mol polysaccharide is composed of glucose, galactose, glucuronic acid, galacturonic acid, and contains a single branched ramification and one sulphate group. The second step was the preparation of low molecular weight bioactive polysaccharides presenting interest...
Tipo: Text Palavras-chave: Phosphorylation; Sulphation; Depolymerization; NMR spectroscopy; Structure; Deep sea hydrothermal vent; Bacteria; Exopolysaccharides; Activité anticoagulante; Phosphatation; Sulfatation; Dépolymérisation; Spectroscopie de RMN; Structure; Sources hydrothermales; Bactéries; Exopolysaccharides.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/these-622.pdf
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Identification of a protein kinase activity that phosphorylates connexin43 in a pH-dependent manner BJMBR
Yahuaca,P.; Ek-Vitorin,J.F.; Rush,P.; Delmar,M.; Taffet,S.M..
The carboxyl-terminal (CT) domain of connexin43 (Cx43) has been implicated in both hormonal and pH-dependent gating of the gap junction channel. An in vitro assay was utilized to determine whether the acidification of cell extracts results in the activation of a protein kinase that can phosphorylate the CT domain. A glutathione S-transferase (GST)-fusion protein was bound to Sephadex beads and used as a target for protein kinase phosphorylation. A protein extract produced from sheep heart was allowed to bind to the fusion protein-coated beads. The bound proteins were washed and then incubated with 32P-ATP. Phosphorylation was assessed after the proteins were resolved by SDS-PAGE. Incubation at pH 7.5 resulted in a minimal amount of phosphorylation while...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Connexin; Phosphorylation; Phosphotransferases; Protein kinase.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400005
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Phosphorylated and cross-linked wheat starches in the presence of polyethylene oxide and their application in biocomposite films. Repositório Alice
BRUNI, G. P.; OLIVEIRA, J. P. de; EL HALAL, S. L. M.; FLORES, W. H.; GUNDEL, A.; MIRANDA, M. Z. de; ZAVAREZE, E. da R.; DIAS, A. R. G..
bitstream/item/178159/1/ID44332-2018mar9pStarch.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Trigo; Biocomposites; Cross-linking reagents; Phosphorylation; Polyethylene; Wheat starch.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1092247
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Posttranslational protein S-palmitoylation and the compartmentalization of signaling molecules in neurons Biol. Res.
PATTERSON,SEAN I.
Protein domains play a fundamental role in the spatial and temporal organization of intracellular signaling systems. While protein phosphorylation has long been known to modify the interactions that underlie this organization, the dynamic cycling of lipids should now be included amongst the posttranslational processes determining specificity in signal transduction. The characteristics of this process are reminiscent of the properties of protein and lipid phosphorylation in determining compartmentalization through SH2 or PH domains. Recent studies have confirmed the functional importance of protein S-palmitoylation in the compartmentalization of signaling molecules that support normal physiological function in cell division and apoptosis, and synaptic...
Tipo: Journal article Palavras-chave: S-palmitoylation; Synapse; Growth cone; Regeneration; Phosphorylation.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200005
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Retinol-induced changes in the phosphorylation levels of histones and high mobility group proteins from Sertoli cells BJMBR
Moreira,J.C.F.; Dal-Pizzol,F.; Rocha,A.B.; Klamt,F.; Ribeiro,N.C.; Ferreira,C.J.S.; Bernard,E.A..
Chromatin proteins play a role in the organization and functions of DNA. Covalent modifications of nuclear proteins modulate their interactions with DNA sequences and are probably one of the multiple factors involved in the process of switch on/off transcriptionally active regions of DNA. Histones and high mobility group proteins (HMG) are subject to many covalent modifications that may modulate their capacity to bind to DNA. We investigated the changes induced in the phosphorylation pattern of cultured Wistar rat Sertoli cell histones and high mobility group protein subfamilies exposed to 7 µM retinol for up to 48 h. In each experiment, 6 h before the end of the retinol treatment each culture flask received 370 KBq/ml [32P]-phosphate. The histone and HMGs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sertoli cells; Phosphorylation; Histones; High mobility group proteins; Retinol.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300005
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TNNI3K is a novel mediator of myofilament function and phosphorylates cardiac troponin I BJMBR
Wang,Hui; Wang,Lin; Song,Li; Zhang,Yan-Wan; Ye,Jue; Xu,Rui-Xia; Shi,Na; Meng,Xian-Min.
The phosphorylation of cardiac troponin I (cTnI) plays an important role in the contractile dysfunction associated with heart failure. Human cardiac troponin I-interacting kinase (TNNI3K) is a novel cardiac-specific functional kinase that can bind to cTnI in a yeast two-hybrid screen. The purpose of this study was to investigate whether TNNI3K can phosphorylate cTnI at specific sites and to examine whether the phosphorylation of cTnI caused by TNNI3K can regulate cardiac myofilament contractile function. Co-immunoprecipitation was performed to confirm that TNNI3K could interact with cTnI. Kinase assays further indicated that TNNI3K did not phosphorylate cTnI at Ser23/24 and Ser44, but directly phosphorylated Ser43 and Thr143 in vitro. The results obtained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: TNNI3K; CTnI; Phosphorylation; Contraction function.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200128
Registros recuperados: 11
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