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Han,; Kim, Ji Hyung; Renault, Tristan; Choresca, Casiano, Jr.; Shin, Sang Phil; Jun, Jin Woo; Park, Se Chang. |
Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA,... |
Tipo: Text |
Palavras-chave: Cyprinid herpes virus 3; Glycoprotein gene; Koi herpesvirus; Microsatellite; Polymorphism; Phylogenetic tree. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00118/22940/20768.pdf |
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Giaretta,Diorvania Ribeiro; Bogo,Amauri; Coelho,Cileide Maria Medeiros; Guidolin,Altamir Frederico; Dantas,Adriana Cibele de Mesquita; Gomes,Eliane Aparecida. |
Several diseases have affected apple production, among them there is Glomerella leaf spot (GLS) caused by Colletotrichum spp. The first report of this disease in apple was in plants nearby citrus orchards in São Paulo State, Brazil. The origin of this disease is still not clear, and studies based on the molecular phylogeny could relate the organisms evolutionarily and characterize possible mechanisms of divergent evolution. The amplification of 5.8S-ITS (Internal Transcribed Spacer) of rDNA of 51 pathogenic Colletotrichum spp. isolates from apples, pineapple guava and citrus produced one fragment of approximately 600 bases pairs (bp) for all the isolates analyzed. The amplified fragments were cleaved with restriction enzymes, and fragments from 90 to 500bp... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pineapple guava; Citrus; Molecular phylogeny; In silico; Phylogenetic tree. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400010 |
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Abreu,JT; Mourão,MM; Santos,CE; Veloso,CJM; Resende,JS; Flatschart,RB; Folgueras-Flatschart,AV; Júnior,SN; Santoro,MM; Mendes,ACR; Franco,GR; Silva,A; Campos,AB; Fernandez,S. |
Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infectious bronchitis virus; Genotyping; Spike glycoprotein; Nucleocapsid; Phylogenetic tree. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200005 |
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Wanntorp, L.. |
Hoya with perhaps 300 species is one of the most diverse angiosperm genera in the tropical rainforests of Southeast Asia and New Guinea. Knowledge of the number of species and their relationships is still very unsatisfactory. Floral characters have been used to subdivide the genus into sections, although none of these groups were based on phylogenetic relationships. Recently, molecular phylogenetic studies have shed new light on the intrageneric relationships of the genus by identifying three main monophyletic clades, in addition to a core Hoya group that includes most of the species examined. One clade includes New Guinean/Australian species, while the other two, the Acanthostemma and the Eriostemma clades, mainly correspond to existing sections. I here... |
Tipo: Article / Letter to the editor |
Palavras-chave: Acanthostemma; Core Hoya; Eriostemma; Hoya; Molecular systematics; New Guinean/Australian clade; Phylogenetic tree. |
Ano: 2009 |
URL: http://www.repository.naturalis.nl/record/526034 |
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Bai,Jun Yan; Wang,Xu; Yang,You Bing; Zhang,Xiao Hui; Pang,You Zhi; Li,Hong Wei. |
ABSTRACT In this study, POU1F1 gene polymorphism was detected in five sheep populations (large-tailed Han, small-tailed Han, Yuxi fat-tailed, Lanzhou large-tailed, and Mongolian sheep), using DNA pooling and sequencing, to provide theoretical basis for the breeding of excellent sheep varieties. Three single-nucleotide polymorphism (SNP) loci of POU1F1 gene were detected in five sheep populations, namely C355T (C/T), C71G (C/G), and C330G (C/G). C and T frequencies of C355T were 0.67/0.33, 0.81/0.19, 0.67/0.33, 1.00/0.00, and 0.93/0.07, respectively, in large-tailed Han, small-tailed Han, Yuxi fat-tailed, Mongolian, and Lanzhou large-tailed sheep. C of C355T locus was the dominant allele in five sheep populations. C and G allele frequencies of C330G locus... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA pooling; Phylogenetic tree; Sheep; SNP. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016001000604 |
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