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Activity of antioxidant enzymes in leaves and bracts of sunflower (Helianthus annuus L.) Eprints@IARI
Singh, D V; Sairam, R K; Srivastava, G C.
A study was conducted to examine the activity of superoxide dismutase (SOD) and its isozymes, ascorbate peroxidase (APOX) and peroxidase (POX) in bracts and in the upper most 1st and 3rd leaves of sunflower at flower opening stage (50 days after sowing) and seed filling stage (70 DAS). The upper most leaf has smaller amounts of chlorophyll and carotenoids and lower activity of SOD, its isozymes, ascorbate peroxidase and peroxidase than the third leaf at flower-opening stage but increased significantly at seed filling stage. Relative water content (RWC) did not differ significantly in any of the leaves at the two stages. Soluble protein content did not differ at the 1st stage but was higher in 1st leaf at seed filling stage. Bracts in upper and lower rows...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2004 URL: http://eprints.iari.res.in/168/1/IJPP-Sunflower_senescence.pdf
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Behavior of photosynthetic products associated with growth and grain production in the rice plant International Rice Research Institute
Lian, Shen.
76 leaves : photos. Thesis (M.S.) -- University of the Philippines
Tipo: Thesis Palavras-chave: Rice; Plant Physiology; Photosynthesis; Plant growth.
Ano: 1965 URL: http://hdl.handle.net/123456789/439
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Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress Eprints@IARI
Sairam, R K; Srivastava, G C.
Effects of long term, medium level (electrical conductivity of extract (ECe)/6.85 dS m1) sodium chloride (NaCl) salinity were studied in tolerant (Kharchia 65) and susceptible (HD 2687) wheat genotypes. NaCl salinity caused decrease in relative water content (RWC), chlorophyll (CHL), membrane stability index (MSI) and ascorbic acid (AA) content, and increased the contents of hydrogen peroxide (H2O2), thiobarbituric acid reactive substances (TBARS) (measure of lipid peroxidation) and activities of superoxide dismutase (SOD), its various isozymes, ascorbate peroxidase (APOX) and glutathione reductase (GR) in wheat genotypes Kharchia 65 (tolerant) and HD 2687 (susceptible). Salinity tolerant wheat cv. Kharchia 65 showed less decline in RWC, CHL, MSI estimated...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2002 URL: http://eprints.iari.res.in/216/1/Plant_Science-RKS-Salinity.pdf
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Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes Eprints@IARI
Agarwal, S; Sairam, R K; Srivastava, G C; Meena, R C.
Abscisic acid (ABA) and salicylic acid (SA) were sprayed on leaves of wheat genotypes C 306 and Hira at 25 and 40 d after sowing under moderate water stress (-0.8 MPa) imposed by adding PEG-6000 in nutrient solution. ABA and SA increased the activities of superoxide dismutase, ascorbate peroxidase, glutathione reductase, and catalase in comparison to unsprayed control plants. Both ABA and SA treatments decreased the contents of hydrogen peroxide and thiobarbituric acid reactive substances, a measure of lipid peroxidation, compared to unsprayed plants. The beneficial effect of increase in antioxidant enzymes activity and decrease in oxidative stress was reflected in increase in chlorophyll and carotenoid contents, relative water content, membrane stability...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2005 URL: http://eprints.iari.res.in/156/1/Biol._Plant.-2005-ABA-SA-Sarika.pdf
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CHANGES IN PHOTOSYNTHESIS RELATED PARAMETERS IN ISABGOL (PLANTAGO OVATA) UNDER DOWNY MILDEW INFECTION Eprints@IARI
R, Saravanan; Mandal, Kunal; Gutam, Sridhar; Samanta, Jatindra Nath; V, Saroj Kumar.
Sequential changes in leaf chlorophyll content and associated gas exchange parameters in isabgol (Plantago ovata) were recorded over a period of nine days starting from initial sporulation to severe chlorosis in a highly downy mildew susceptible cultivar (Niharika). The Downy mildew caused significant reduction in chlorophyll content. The loss of chlorophyll was more severe in case of chlorophyll ‘a’ compared to chlorophyll ‘b’. Net photosynthetic rate and stomatal conductance in the diseased leaves also reduced compared to healthy ones. However, internal CO2 concentration (Ci) increased in diseased leaves. Difference between healthy and infected leaves in terms of stomatal conductance and Ci became significant at ninth day of study. In the complex...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2010 URL: http://eprints.iari.res.in/281/1/PPS_08.pdf
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Chemical Manipulation of Reproductive Efficiency of Chickpea (Cicer arietinnum L.) by Triadimefon in Relation to Changes in Ethylene Eprints@IARI
Saxsena, D C; Abbas, Sabra; Sairam, R K.
Reproductive efficiency was successfully manipulated by the application of different concentrations of triadimefon at pre-flowering, flowering and post-flowering stages in chickpea (Cicer arietinum L.). Triadimefon improved the parameters determining reproductive efficiency. Yield was significantly improved due to reductions in flower and pod abscission by the application of triadimefon, which also lowered the ethylene levels in leaves, flowers and pods and delayed senescence. These findings suggest a relationship between ethylene level and reproductive efficiency in chickpea. Further, they indicate that triadimefon can be used for chemical manipulation of the reproductive efficiency of chickpea under field conditions.
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2000 URL: http://eprints.iari.res.in/213/1/JACS-Saxena-2000.pdf
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CO2-mediated changes in mungbean chemistry: Impact on plant–herbivore interactions Eprints@IARI
Srivastav, A C; Tiwari, L D; Pal, Madan; Sengupta, U K.
The effects of long-term CO2 enrichment on the foliar chemistry of mungbean (Vigna radiata L. Wilczek) and the consequences of these chemical changes on the performance of the tobacco caterpillar (Spodoptera litura) were investigated. Foliage was collected from plants grown for kharif season under ambient or enriched CO2 (average 350 ± 25 and 600 ± 50 μl l–1, respectively) in outdoor, open top chambers at the Plant Physiology Division, Indian Agricultural Res- earch Institute, New Delhi. Under enriched CO2, level of leaf nitrogen (protein and non-protein nitrogen) declined and levels of starch and total soluble sugars (reducing and non-reducing) in leaves increased. Long-term bioassay (12 days) of tobacco caterpillar revealed that feeding by herbivores on...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2009 URL: http://eprints.iari.res.in/119/1/1148.pdf
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Combining ability for superoxide dismutase, peroxidase and catalase enzymes in cabbage head (Brassica oleracea var. capitata L.) Eprints@IARI
Singh, B.K.; Sharma, S.R.; Singh, Bhupinder.
Antioxidant enzymes have been touted as beneficial for enhancing the fitness, preventing disorders, and mitigating the effects of aging and senescence. Our objective was to evaluate combining ability of superoxide dismutase, peroxidase, and catalase activity in cabbage head. Head samples were frozen immediately in liquid nitrogen and placed at 80 8C for assay. Less than unity values of s2 gca/s2 sca ratio for all three enzymes indicated predominance of non-additive gene action. The parents CMS-GA and Red Cabbage excelled as good general combiners for all antioxidants and indicated the value and need for multiple crossing. The crosses CMS-GA x Red Cabbage, CMS-GA x C-2, 83-2 x AC-204, 83-2 x EC-490174, 83-2 x AC-1021, Pride of Asia x C-4, and Pride of Asia...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2009 URL: http://eprints.iari.res.in/312/1/binod_et_al_2009_scientia_hort.pdf
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Comparison of hexaploid and tetraploid wheat cultivars in their responses to water stress Eprints@IARI
Sairam, R K; Chandrasekhar, V; Srivastava, G C.
We studied the effect of water stress imposed at anthesis and pre-anthesis stages on oxidative stress and antioxidant activity in four wheat cultivars, two hexaploid Triticum aestivum cultivars, drought resistant cv. C 306 and drought susceptible cv. Hira, and two tetraploid cultivars, T. durum cv. A 9-30-1 and T. dicoccum cv. HW 24. Water stress decreased relative water content (RWC), membrane stability index (MSI), and increased H2O2 and malondialdehyde (MDA) contents as well as activity of superoxide dismutase (SOD), catalase (Cat) and peroxidase (POX) in all the genotypes at all the stages. Both the tetraploid cultivars showed higher RWC, MSI and SOD activity, and lower H2O2 and MDA contents under water stress than hexaploid ones. Cat and POX...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2001 URL: http://eprints.iari.res.in/162/1/Biol-Plant-2001-Chandrasekar.pdf
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Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes Eprints@IARI
Sairam, R K; Srivastava, G C; Agarwal, S; Meena, R C.
Effects of long-term sodium chloride salinity (100 and 200 mM NaCl; ECe = 6.85 and 12.3 dS m-1) were studied in tolerant (Kharchia 65, KRL 19) and susceptible (HD 2009, HD 2687) wheat genotypes. NaCl decreased relative water content (RWC), chlorophyll content (Chl), membrane stability index (MSI) and ascorbic acid (AA) content, and increased the contents of hydrogen peroxide, thiobarbituric acid reactive substances (TBARS), and activities of superoxide dismutase (SOD), ascorbate peroxidase (APOX) and glutathione reductase (GR). Kharchia 65 showed lowest decline in RWC, Chl, MSI and AA content, lowest increase in H2O2 and TBARS contents and higher increase in SOD and its isozymes, APOX and GR, while HD2687 showed the highest decrease in AA content, highest...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2005 URL: http://eprints.iari.res.in/157/1/Biol._Plant.-2005--Salinity.pdf
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Differences in Root Exudation Among Phosphorus-Starved Genotypes of Maize and Green Gram and Its Relationship with Phosphorus Uptake Eprints@IARI
Singh, Bhupinder; Pandey, Renu.
Availability of phosphorus (P) in soil and its acquisition by plants is affected by the release of high and low molecular weight root exudates. A study was carried out to ascertain the qualitative and quantitative differences in root exudation among the genotypes of maize (Zea mays L.) and green gram (Vigna radiata L.) under P-stress. Results showed that both inter- and intra-species differences do exist among maize and green gram in terms of root exudation, P uptake, and shoot and root P content. In general, green gram, a legume crop, had greater root exudation compared to maize. However, the amino acid content of the total root exudates in maize was two-fold as compared to green gram. The maize and green gram genotypes possessed genetic variability in...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2003 URL: http://eprints.iari.res.in/247/1/Root_exudation_in_maize_%26_mungbean_Singh_%26_pandey_JPN-2003.pdf
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Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration Eprints@IARI
Sairam, R K; Veerabhadra Rao, K; Srivastava, G C.
Effect of long term soil salinity was studied in wheat cvs, Kharchia 65 (tolerant) and KRL 19 (moderately tolerant) under control and two levels of salinity (ECe/5.4 and 10.6 dS m1). Salinity stress decreased relative water content (RWC), chlorophyll (CHL), carotenoids (CAR), membrane stability index (MSI), biomass and grain yield, and increased hydrogen peroxide (H2O2), thiobarbituric acid reactive substances (TBARS), proline, glycine-betaine (GB), soluble sugars, superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) activity in both the genotypes and at all the stages. Salinity induced decrease in RWC, CHL, CAR, MSI, biomass and grain yield were significantly higher in KRL 19 than more tolerant Kharchia 65. Kharchia 65 recorded higher...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2002 URL: http://eprints.iari.res.in/212/1/Plant_Sci.-Rao-Salinity.pdf
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Effect of 5-sulfosalicylic acid on antioxidant activity in relation to vase life of Gladiolus cut flowers Eprints@IARI
Ezhilmathi, K; Singh, V P; Arora, Ajay; Sairam, R K.
An experiment was conducted to study the effect of 5-sulfosalicylic acid (5-SSA) on the vase life of cut flowers of Gladiolus grandiflora variety ‘Green Willow’. The vase solution having 5-SSA significantly increased cumulative uptake of vase solution, vase life, number of opened florets and decreased the number of unopened florets compared to the controls. Spikes kept in vase solution containing 5-SSA also exhibited lower respiration rates, lipid peroxidation and lipoxygenase (LOX) activity, and higher membrane stability, soluble protein concentration, and activity of superoxide dismutase (SOD) and catalase. Results suggest that 5-SSA increases vase life by increasing the reactive oxygen species (ROS) scavenging activity of the Gladiolus cut flowers.
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2007 URL: http://eprints.iari.res.in/215/1/PGR-Gladiolus-Ezhilmathi.pdf
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EFFECT OF ELEVATED CARBON DIOXIDE ON KINETICS OF NITRATE UPTAKE IN WHEAT ROOTS Eprints@IARI
Lekshmy, S; Vanita, Jain; Sangeeta, Khetarpal; Pandey, Renu; Rajendra, Singh.
Wheat (Triticum aestivum L.) cv PBW 343 was grown in Hoagland solution devoid of nitrogen (-N) and with 1 mM KNO3 (+N) under two carbon dioxide levels, viz. ambient (370 μl/l, AC) and elevated (600±50 μl/l, EC) for twenty days in growth chambers. The pattern of nitrate uptake was similar under both AC and EC but, the rate of uptake was significantly higher in EC grown plants when the concentration of the nitrate in the external medium was low. The Vmax for high affinity nitrate transport system (HATS) was significantly high for the EC grown un-induced seedlings (116.36 μmol g-1 fw h-1) compared with AC grown un-induced seedlings (79.55 μmol g-1 fw h-1). The pattern of gene expression of the nitrate transporters in roots correlated with the kinetics and...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2009 URL: http://eprints.iari.res.in/78/1/Effect_of_eCO2_on_kintics_of_nitrate_uptake_in_wheat-Lekshmy_et_al_2009_IJPP.pdf
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Effect of elevated carbon dioxide on Spinach (Spinacia oleracea L.) plants Eprints@IARI
Jain, Vanita; Saxsena, D C; Pandey, Renu; Sairam, R K.
Spinach is an important leafy vegetable enriched in iron. An experiment was conducted to study the effect of elevated carbon dioxide on growth and nutrient composition of spinach. The plants were grown in open top chambers (OTC) under two concentration of carbon dioxide viz. ambient (ACO2, 350± 50 μmol mol-1) and elevated (ECO2, 600± 50 μmol mol-1) and were analyzed 40, 60 and 80 days after exposure to CO2. The plants grown in ECO2 had higher net photosynthetic rate and lower stomatal conductance when compared with the plants grown in ACO2. ECO2 also changed the nutrient composition of leaves. Lower nitrogen and iron content and higher carbon and calcium content were observed in the plants exposed to elevated CO2. The rising CO2 may lead to changes in...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2009 URL: http://eprints.iari.res.in/277/1/eCO2___spinach_plants_hindi_article_Jain-saxena-pandey-sairam.pdf
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Effect of ethrel on reproductive efficiency in chickpea Eprints@IARI
Saxsena, D C; Abbas, Sabra; Sairam, R K.
An experiment was conducted with chickpea (Cicer arietinum L.) cultivar BG 209 to study the effect of ethrel applied as foliar spray at low (250 ppm), medium (500 ppm) and high (1000 ppm) concentrations at pre flowering (65 DAS: S1), mid-flowering (94 DAS: S2) and post-flowering (125 DAS: S 3) stages. The low and medium concentrations resulted in increase in dry matter over the control plants, though medium concentration was less effective than lower concentration at all the stages. Maximum leaf area was observed at lowest ethrel concentration at all stages and there was decline in leaf area with increasing ethrel concentration. Low concentration increased the pod number at S1 (17%) and S2 stages (11%), while at S3 stage, a 22% reduction in pod number was...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2007 URL: http://eprints.iari.res.in/165/1/IJPP-Ethrel.pdf
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Effect of Homobrassinolide Application on Metabolic Activity and Grain Yield of Wheat under Normal and Water-Stress Condition Eprints@IARI
Sairam, R K.
Activities of nitrate reductase, glutamine synthetase along with rate of photosynthesis, chlorophyll content, soluble protein, relative water content, transpiration and diffusion resistance were estimated in wheat cv. C306 at anthesis stage under irrigated and moisture-stress condition and two levels (0.1 and 1.0 ppm) of homobrassinolide application. Yield and yield attributing parameters were recorded at harvest. Moisturestress adversely affected relative water content, transpiration, net photosynthesis, nitrate reductase and glutamine synthetase activity, chlorophyll and soluble protein content. Homobrassinolide application increased leaf relative water content and transpiration and decreased diffusion resistance in water-stressed and recovered plants....
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 1994 URL: http://eprints.iari.res.in/204/1/JACS-HBR.pdf
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Effect of low dose gamma irradiation on plant and grain nutrition of wheat Eprints@IARI
Singh, Bhupinder; Datta, P.S..
We recently reported the use of low dose gamma irradiation to improve plant vigor, grain development and yield attributes of wheat (Singh and Datta, 2010). Further, we report here the results of a field experiment conducted to assess the effect of gamma irradiation at 0, 0.01, 0.03, 0.05, 0.07 and 0.1 kGy on flag leaf area, stomatal conductance, transpiration and photosynthetic rate and plant and grain nutritional quality. Gamma irradiation improved plant nutrition but did not improve the nutritional quality of grains particularly relating to micronutrients. Grain carotene, a precursor for vitamin A, was higher in irradiated grains. Low grain micronutrients seem to be caused by a limitation in the source to sink nutrient translocation rather than in the...
Tipo: Article Palavras-chave: Agricultural Physics; Plant Genetic Resources; Plant Physiology.
Ano: 2010 URL: http://eprints.iari.res.in/304/1/Wheat_Paper_RPC5041-_final_pdf.pdf
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EFFECT OF REDUCED SEED AND APPLIED ZINC ON ZINC EFFICIENCY OF WHEAT GENOTYPES UNDER ZINC DEFICIENCY IN NUTRIENT SOLUTION CULTURE Eprints@IARI
Singh, Bhupinder; Singh, B.K..
Improving zinc efficiency of cereals especially wheat under zinc deficiency is a priority area of research to determine and manipulate the plant factors that govern zinc uptake and utilization. Experiments conducted to ascertain variability and also the contribution of seed zinc (complete seed and half seed) to zinc efficiency in diverse wheat genotypes raised on zinc sufficient and deficient nutrient solution, showed wide genetic variability and general deficiency response such as, increase in root to shoot ratio and decrease in leaf chlorophyll, superoxide dismutase activity, total plant zinc concentration, and uptake. Since difference in zinc deficiency response were more distinct at the initial growth stage, seed zinc could be crucial for determining...
Tipo: Article Palavras-chave: Plant Physiology; Soil Science.
Ano: 2011 URL: http://eprints.iari.res.in/316/1/Bhupi_binod_2011.pdf
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Effect of waterlogging on carbohydrate metabolism in pigeon pea (Cajanus cajan L.): Upregulation of sucrose synthase and alcohol dehydrogenase Eprints@IARI
Kumutha, D; Sairam, R K; Ezhilmathi, K; Chinnuswamy, V; Meena, R C.
The objective of this study was to examine the role of root carbohydrate levels and metabolism in the waterlogging tolerance of contrasting pigeon pea genotypes. An experiment was conducted with 4 pigeon pea (Cajanus cajan) genotypes, 2 tolerant (ICPL 84023 and ICP 301) and 2 susceptible (ICP 7035 and Pusa 207) to waterlogging stress.Waterlogging treatment was given by placing pots with 25 days old plants in plastic troughs filledwithwater. Waterlogging resulted in decrease in leaf area, dry matter, relative water content and chlorophyll content in leaves, andmembrane stability index in root and leaf tissues. The decline was greater in ICP 7035 and Pusa 207, which also suffered almost 100% mortality during recovery of 6 days waterlogged plants, than ICPL...
Tipo: Article Palavras-chave: Plant Physiology.
Ano: 2008 URL: http://eprints.iari.res.in/205/1/JACS-Oxidative_-2000.pdf
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