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Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene J. Venom. Anim. Toxins incl. Trop. Dis.
Carnieli JR,P.; Ventura,A. M.; Durigon,E. L..
A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabies; G gene; Plasmids; Transfection.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400015
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Découverte et caractérisation des premiers virus de Thermotogales (bactéries thermophiles et anaérobies) issus de sources hydrothermales océaniques profondes ArchiMer
Loussouarn, Julien.
Our knowledge of the viral diversity associated to procaryotic microorganisms inhabiting the deep sea hydrothermal vents is still limited. Only few studies have focused on viral abundance and impact on microbial mortality within these ecosystems. A limited number of viruses from these environments were isolated and characterized. Two viruses, PAV1 and TPV1, associated to hyperthermophilic anaerobic Archaea, Thermococcales order, have ever been described in our laboratory. The topic of this phD thesis was to extend our investigation to other deep sea vent microorganisms in order to deepen our knowledge on the marine hydrothermal virosphere. We decided to focus more precisely on the bacterial order of Thermotogales. This order is composed of anaerobic...
Tipo: Text Palavras-chave: Sources hydrothermales océaniques profondes; Siphovirus; Plasmides; Piratage moléculaire; Plasmids; Molecular piracy; Deep sea hydrothermal vents; Thermotogales; Siphoviruses.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00609/72137/70889.pdf
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Detection and characterization of bacteriocin-producing Lactococcus lactis strains Rev. Microbiol.
Moreno,Izildinha; Lerayer,Alda L.S.; Leitão,Mauro F. de Freitas.
One hundred sixty seven strains of Lactococcus lactis were screened for bacteriocin production by well diffusion assay of GM17 agar. Fourteen (8.4%) produced antimicrobial activity other than organic acids, bacteriophages or hydrogen peroxide. The frequency of bacteriocin production ranged from 2% in L. lactis subsp. cremoris up to 12% in L. lactis subsp. lactis. Antimicrobial activities were not observed in any strain of L. lactis subsp. lactis var. diacetylactis. Among thirteen bacteriocin-producing strains and two nisin-producing strains (L. lactis subsp. lactis ATCC 11454 and L. lactis subsp. lactis CNRZ 150), eight (53%) were characterized as lactose-positive (Lac+) and proteinase-negative (Prt-). The bacteriocin-producing cultures were also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: L. lactis; Bacteriocins; Fermentation; Plasmids.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200008
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Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens BJMBR
Carbonell,G.V.; Alfieri,A.F.; Alfieri,A.A.; Vidotto,M.C.; Levy,C.E.; Darini,A.L.C.; Yanaguita,R.M..
Cytotoxin production was studied in 60 Serratia marcescens strains isolated from hospitalized patients. Association of cytotoxic activity with serotype, source of isolation and presence of plasmids was also evaluated. Thirteen of the 60 S. marcescens strains produced a cytotoxic effect on Vero cells. These strains were isolated from distinct clinical sources and classified into seven different serotypes (O1:H7; O4:NM; O10:NT; O19:NM; O6,14:H4; O6,14:NM and O6,14:H1). No relationship was observed between cytotoxic activity and clinical source or serotypes of the strains. Plasmids from five cytotoxin-producing S. marcescens strains were transferred to E. coli K12/711. The transconjugants did not exhibit cytotoxicity, indicating that the cytotoxic effect is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytotoxin production; Nosocomial infections; Plasmids; Serratia marcescens; Virulence factors.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100005
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Evaluation of anti-Wnt/β-catenin signaling agents by pGL4-TOP transfected stable cells with a luciferase reporter system BJMBR
Chuang,K.A.; Lieu,C.H.; Tsai,W.J.; Wu,M.H.; Chen,Y.C.; Liao,J.F.; Wang,C.C.; Kuo,Y.C..
Refractory and relapsed leukemia is a major problem during cancer therapy, which is due to the aberrant activation of Wnt/β-catenin signaling pathway. Activation of this pathway is promoted by wingless (Wnt) proteins and induces co-activator β-catenin binding to lymphoid enhancer factor (LEF)/T-cell factor protein (TCF). To provide a convenient system for the screening of anti-Wnt/β-catenin agents, we designed a bi-functional pGL4-TOP reporter plasmid that contained 3X β-catenin/LEF/TCF binding sites and a selectable marker. After transfection and hygromycin B selection, HEK 293-TOP and Jurkat-TOP stable clones were established. The luciferase activity in the stable clone was enhanced by the recombinant Wnt-3A (rWnt-3A; 100-400 ng/mL) and GSK3β inhibitor...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cancer; Plasmids; Reporter assay; Screening; Wnt; Β-catenin.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000003
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Extending the repertoire of microsatellite markers for genetic linkage mapping and germplasm screening in chickpea Open Agri
Varshney, R.K.,( Rajeev K. Varshney ).
Palavras-chave: Genetic structures; Chickpeas; Genomes; Cloning; Chromosome mapping; Plasmids; Polymorphism; DNA; Genotypes; Genetic markers.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3338
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Frequency of plasmid-mediated AmpC in Enterobacteriaceae isolated in a Brazilian Teaching Hospital BJM
Campana,Eloiza H.; Barbosa,Paula P.; Fehlberg,Lorena C.C.; Gales,Ana C..
In Brazil, the presence of plasmid-mediated AmpC (pAmpC)-producing isolates has been sporadically reported. We evaluated the frequency of pAmpC among 133 Enterobacteriaceae clinical isolates. The blaCMY-2-like gene was detected in a single Klebsiella pneumoniae isolate. In our study, the pAmpC frequency was very low as previously reported.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Plasmids; Beta-lactamases; Beta-lactams; Brazil.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200023
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Homology among extra-cryptic DNA bands and the typical plasmids in Brazilian Yersinia pestis strains BJM
Leal,Nilma Cintra; Sobreira,Marise; Leal,Tereza Cristina Arcanjo; Almeida,Alzira Maria Paiva de.
Yersinia pestis, the etiologic agent of plague, harbors three well-characterized plasmids: pFra (90-110kb), pYV (70 kb) and pPst (9.5 kb). Furthermore, some extra-cryptic DNA bands have been observed in a number of wild strains from several foci of the world. Additional bands have also been reported in Brazilian strains. Looking for any relationship among these cryptic DNA bands and the three-prototypical plasmids, we analyzed twelve strains displaying different plasmid content. The DNA bands were hybridized by southern blot with probes directed at the genes caf1, lcrV and pla located respectively on the plasmids pFra, pYV and pPst. The probes were constructed by PCR amplification and labeled with digoxigenin. The Pla probe hybridized with its target...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Y. pestis; Plasmids; Probes; Virulence genes.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100006
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New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR. Repositório Alice
VENTURELLI, G. L.; SILVSA, K. J. da; TREML, D.; NAVAS, P. B.; VARGAS, M. O.; BISCHOFF, J. L.; FARIA, J. C. de; ARISI, A. C. M..
The geminivirus-resistant common bean, Embrapa 5.1, was the first commercial genetically modified (GM) event developed in Latin America by the Brazilian Agricultural Research Corp.. Therein novel standard reference plasmids were constructed for species-specific (pLEC) and event-specific (pFGM) qualitative and quantitative detection of Embrapa 5.1 GM common bean. To establish these plasmids as certified reference materials (CRM) for Embrapa 5.1 GM common bean, two DNA extraction protocols, simplex and duplex qPCR using two different operators (experimenters) were tested. The efficiency values ranged from 92% to 110% for the simplex and duplex reactions considering both operators. The limit of detection was enough to detect at least 0.1% GM content. These...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Certified reference material; EMB-PV051-1-event; Feijão; Phaseolus Vulgaris; Organismo Transgênico; Engenharia Genética; Plasmídeo; Beans; Plasmids; Genetically modified organisms; Quantitative polymerase chain reaction.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100557
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Stability analysis of chickpea large genomic DNA inserts in Agrobacterium Open Agri
Rajesh, P.N..
Palavras-chave: Cloning; Genomes; Plasmids; DNA; Chickpeas; Genetic transformation; Chromosomes; Antibiotics; Bacteria; Genes.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3293
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Vibrio nigripulchritudo monitoring and strain dynamics in shrimp pond sediments ArchiMer
Walling, Emilie; Vourey, Elodie; Ansquer, Dominique; Beliaeff, Benoit; Goarant, C..
Aims: A description of bacterial pathogens in shrimp ponds is necessary to understand their pathogenesis. Vibrio nigripulchritudo was shown to contain saprophytic and pathogenic strains among New Caledonian isolates. We established a method to map the development of V. nigripulchritudo in pond sediments at three different genetic levels: the species level, then at the pathogenic cluster level and finally at the plasmid level, present only in all highly pathogenic isolates. Methods and Results: PCR methods were applied to shrimp pond sediments both before and after a mortality outbreak. Using crude samples, the species V. nigripulchritudo is not detected at first (0/42 samples at day 56 post stocking) but appears frequently in the sediments after the...
Tipo: Text Palavras-chave: Detection; Diagnosis; Diseases; Ecology; PCR; Plasmids; Soil; Virulence.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00004/11484/8177.pdf
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Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil BJM
Costa,Mateus Matiuzzi da; Drescher,Guilherme; Maboni,Franciele; Weber,Shana; Botton,Sônia de Avila; Vainstein,Marilene Henning; Schrank,Irene Silveira; Vargas,Agueda Castagna de.
The present study determined the molecular and resistance patterns of E. coli isolates from urinary tract of swine in Southern of Brazil. Molecular characterization of urinary vesicle samples was performed by PCR detection of virulence factors from ETEC, STEC and UPEC. From a total of 82 E. coli isolates, 34 (38.63%) harbored one or more virulence factors. The frequency of virulence factors genes detected by PCR were: pap (10.97%), hlyA (10.97%), iha (9.75%), lt (8.53%), sta (7.31%) sfa (6.09%), f4 (4.87%), f5 (4.87%), stb (4.87%), f6 (1.21%) and f41 (1.21%). Isolates were resistant to penicillin (95.12%), lincomycin (93.9%), erythromycin (92.68%), tetracycline (90.24%), amoxicillin (82.92%), ampicillin (74.39%), josamycin (79.26%), norfloxacin (58.53%),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plasmids; Virulence factors; Antimicrobial resistance; Swine.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400027
Registros recuperados: 12
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