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| SOARES,A. M.; ANZALONI PEDROSA,L. H.; FONTES,M. R. M.; DA SILVA,R. J.; GIGLIO,J. R.. |
| Polyacrylamide gel electrophoresis (PAGE) for basic proteins may be a useful tool for the characterization of whole snake venoms and for the taxonomic classification of snakes of the Elapidae and Viperidae families. However, due to the close proximity of molecular weights among toxins bearing different net charges, sodium dodecyl sulfate (SDS) - PAGE was not able to provide an efficient differentiation. This article reports the electrophoretic analysis of several venoms from the genera Micrurus, Bothrops, Bothriopsis, Crotalus and Lachesis and shows a typical and distinctive electrophoretic profile for each species, with intraspecific and geographic variations. Even in cases in which extreme morphological similarities were present, such as between B.... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Snake venoms; Basic toxins; Polyacrylamide gel electrophoresis; Elapidae; Viperidae. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000200005 |
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| Filippo,P.A. Di; Viana,I.S.; Almeida,A.J.; Graça,F.A.S.; Rodrigues,A.B.F.; Nogueira,A.F.S.. |
| ABSTRACT Protein electrophoresis is a relatively simple technique that allows separating serum protein fractions, and provides important information in the investigation and diagnosis of several diseases. This study determined the levels of acute-phase proteins in the serum of healthy, captive emus (Dromaius novaehollandiae). Animals were divided into two groups (n=11 in each) based on age, with 1-year-old and 4-year-old emus. Acute-phase proteins were separated by SDS-PAGE. Ceruloplasmin, transferrin, albumin, haptoglobin, acidic glycoprotein, IgA, and IgG were detected in the serum of all animals. Protein profiles varied significantly with age (P<0.05). Individuals in the 4-year-old emus group had higher values of ceruloplasmin, transferrin, albumin,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acute-phase response; Polyacrylamide gel electrophoresis; Infection; Emus. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000200405 |
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