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Registros recuperados: 7
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American visceral Leishmaniasis: a case report J. Venom. Anim. Toxins incl. Trop. Dis.
Langoni,H.; Lucheis,S. B.; Da Silva,R. C.; Castro,A. P. B.; Paes,A. C..
Visceral leishmaniasis is a zoonotic disease caused by parasites of the Leishmania genus. Dog is the major source of infection to man, especially in urban areas. The authors report a case of visceral leishmaniasis in a pit bull female dog from Bocaina, São Paulo, Brazil. The animal presented clinical signs compatible with leishmaniasis, including skin lesions in the body and partial damage of the external ears. The indirect fluorescent antibody test (IFAT) demonstrated a titer of 1280, and promastigote forms of Leishmania sp were isolated by the culture of bone marrow puncture. Cytological analysis of the lymph node and smear of the bone marrow puncture revealed macrophages containing amastigote forms of Leishmania sp in their inner region. The test of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmania; Zoonosis; Dog; Diagnosis; Hemoculture; Serology; Polymerase chain reaction (PCR).
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300012
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Comparative Evaluation of Mehods for the Diagnosis of Bovine Tritrichomonas foetus Infection OAK
Felleisen, Richard S. J.; Schimid-Lambelet, Natacha; Walubengo, J..
Palavras-chave: Diagnosis; In vitro culture; Polymerase chain reaction (PCR).
Ano: 1997 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/266
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Genetic variability and diversity of mutant rice revealed by quantitative traits and molecular markers Agrociencia
Oladosu,Yusuff; Rafii,Mohd Y.; Abdullah,Norhani; Malek,Mohammad Abdul; Rahim,Harun A.; Hussin,Ghazali; Ismail,Mohd Razi; Latif,Mohammad Abdul; Kareem,Isiaka.
Genetic variability with mutagenic agents has been employed in plant breeding due to its significant contribution to the improvement of the existing rice genotypes by using genetically diverse parents. This study evaluated the genetic variability and diversity of mutant rice using quantitative traits and inter-simple sequence repeats (ISSR) molecular markers. A field experiment was carried out on M5 generation of 31 developed mutant lines and their parent (control). Morphological traits and 25 ISSR molecular markers were used as tools for determining cultivar identities and genetic diversity. The selected primers generated 443 clear polymorphic bands with an average number of 18 per primer. The bands were 85.10 % polymorphic. Un-weighted Pair Group of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant breeding; Genetic diversity; Molecular marker; Mutagenicity; Polymerase chain reaction (PCR).
Ano: 2015 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952015000300002
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POLYMERASE CHAIN REACTION DETECTION OF AVIAN LEUKOSIS VIRUS DNA IN VACCINES USED IN POULTRY Rev Salud Anim.
Acevedo,Ana María; Rodríguez,Edisleidy; Uffo,Odalys; Relova,Damarys; Noda,Julia; Díaz de Arce,Heidy.
Avian leukosis viruses (ALV) provoke a variety of trasmissible bening and malign tumoral diseases affecting birds. Chickens are affected by six subgroups of ALV designs A, B, C, D, E and J of more recent world dissemination. These viruses are potential contaminants of live vaccines used in poultry. In order to research the presence of DNA from ALVs as contaminants of viral commercial vaccines to be used in poultry, different Marek´s disease vaccines were screened by a reported polymerase chain reaction (PCR) assay designed to detect all subgroups of ALVs. DNA samples extracted from seven vaccines were submitted to PCR using primers for a conserved region of env gene of HPRS-103. ALV sequences were detected in seven samples (100%). The methodology employed...
Tipo: Journal article Palavras-chave: Avian leukosis virus (ALV); Polymerase chain reaction (PCR); Vaccine; Contamination; Poultry.
Ano: 2009 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000100011
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Prevalence and quantification of Listeria monocytogenes in beef offal at retail level in Selangor, Malaysia BJM
Kuan,Chee Hao; Wong,Woan Chwen; Pui,Chai Fung; Mahyudin,Nor Ainy; Tang,John Yew Huat; Nishibuchi,Mitsuaki; Radu,Son.
A total of 63 beef offal samples (beef liver = 16; beef lung = 14; beef intestine = 9; beef tripe = 15; beef spleen = 9) from three wet markets (A, B, and C) in Selangor, Malaysia were examined for the prevalence and microbial load of Listeria monocytogenes. A combination of the most probable number and polymerase chain reaction (MPN-PCR) method was employed in this study. It was found that L. monocytogenes detected in 33.33% of the beef offal samples. The prevalence of L. monocytogenes in beef offal purchased from wet markets A, B, and C were 22.73%, 37.50% and 41.18% respectively. The density of L. monocytogenes in all the samples ranged from<3upto&gt; 2,400 MPN/g. The findings in this study indicate that beef offal can be a potential vehicle of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; Most probable number (MPN); Polymerase chain reaction (PCR); Beef offal; Prevalence.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400020
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Rapid and Sensitive Method for Detection of Newly Isolated Babesia Parasite (Babesia sp.1) in the Anticipated Vector-Tick Using the Polymerase Chain Reaction Technique OAK
Ohta, Masato; Kawazu, Shin-ichiro; Tsuji, Naotoshi; Terada, Yutaka; Kamio, Tsugihiko; Fujisaki, Kozo.
Palavras-chave: Babesia ovata; Polymerase chain reaction (PCR); Nested-PCR; Haemaphysalis longicoronis.
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/231
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甜瓜逢機增幅多型性核酸(RAPD)標誌分析及其遺傳特性之研究 Taiwan Agricultural Research Institute
劉邦基; 張有明; 許華欣; 鄭櫻慧; 張慧如; 蕭吉雄; Pan-Chi Liou;You-Ming Chang;Wha-Shin Hsu;Ying-Huey Cheng;Hwei-Ru Chang;Chi-Hsiung Hsiao.
[[abstract]]本研究以一組包含三個世代之甜瓜族群為材料,進行甜瓜的逢機增幅多型性核酸 (random amplified polymorphic DNA, RAPD) 分析,並探討其特性。試驗中證明甜瓜模板核酸在PCR反應前先以限制酶處理會改變RAPD條帶數目,使分析圖譜發生變化,增加多型性之顯示機率,因此可提高分析效率。此種酵素所引起的條帶增減現象極為穩定,其中具有多型性者在後代中之分離情形並符合孟德爾遺傳原理。本報告進一步對酵素處理所引起的圖譜變化提出解釋。配合限制酶的使用,更可證明RAPD基因座之獨立性,增加對RAPD的瞭解。本報告並對RAPD標誌所顯示的甜瓜遺傳特色加以探討,以為遺傳連鎖分析與建立連鎖圖之基礎。
Palavras-chave: 甜瓜 聚合酶連鎖反應 逢機增幅多型性核酸標誌 遺傳分析 Cucumis melo L.; Polymerase chain reaction (PCR); Random amplified polymorphic DNA (RAPD); Genetic analysis [[classification]]6.
Ano: 1998
Registros recuperados: 7
Primeira ... 1 ... Última
 

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