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Registros recuperados: 13
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alpha-Smooth muscle actin and proliferating cell nuclear antigen expression in focal segmental glomerulosclerosis: functional and structural parameters of renal disease progression BJMBR
Geleilete,T.J.M.; Costa,R.S.; Dantas,M.; Coimbra,T.M..
The aim of the present study was to investigate the expression of alpha-smooth muscle actin (alpha-SM-actin) and proliferating cell nuclear antigen (PCNA) in renal cortex from patients with focal segmental glomerulosclerosis (FSGS) and their correlations with parameters of renal disease progression. We analyzed renal biopsies from 41 patients with idiopathic FSGS and from 14 control individuals. The alpha-SM-actin immunoreaction was evaluated using a score that reflected the changes in the extent and intensity of staining in the glomerular or cortical area. The PCNA reaction was quantified by counting the labeled cells of the glomeruli or renal cortex. The results, reported as median ± percentile (25th; 75th), showed that the alpha-SM-actin scores in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpha-smooth muscle actin; Proliferating cell nuclear antigen; PCNA; Focal segmental glomerulosclerosis; Proteinuria; Renal failure; Renal disease progression; Renal fibrosis.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800003
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Apolipoproteína A-I en nefropatías: su relación con el status renal y las microproteínas urinarias ABCL
Madalena,Leticia Bibiana; Facio,María Laura; Bresciani,Pablo Diego; Alejandre,Mariel Emilce; Berg,Gabriela Alicia; Angerosa,Margarita; Pizzolato,Marco Antonio.
El objetivo del presente estudio fue identificar la relación entre la presencia de Apolipoproteína A-I en orina y: a) el daño funcional renal tanto en patología predominantemente glomerular así como túbulo-intersticial; y b) los valores de alfa 1-microglobulina y beta 2-microglobulina, como marcadores de compromiso tubular. Se procesaron 97 orinas de 24 h de recolección. Se determinaron proteínas totales (Exton), alfa 1-microglobulina y beta 2-microglobulina (Inmunoturbidimetría, Roche, USA). Se realizó la técnica de SDS-PAGE, el uroproteinograma con tinción argéntica y el inmunobloteo con anticuerpo anti ApoA-I según procedimientos habituales. Se observó una asociación estadísticamente significativa entre la presencia de ApoA-I y los valores de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apolipoproteína A-I; Alfa 1-microglobulina; Beta 2-microglobulina; Insuficiencia renal; Hematuria; Proteinuria.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572005000300002
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Correlación entre cociente proteína/creatinina y proteinuria de 24 horas en pacientes con enfermedad renal ABCL
Farías,Rosa; Páez,Nancy; Acosta García,Edgar; Marino,Angela; Herrera,Blanca; Padilla,Elio.
La cuantificación de la proteinuria en orina de 24 horas se utiliza con frecuencia para el diagnóstico y la terapéutica en pacientes con enfermedad renal crónica (ERC). Sin embargo, muchas veces se pierde precisión debido a lo complicado que resulta para el paciente la recolección de la muestra. Se ha desarrollado el cociente proteína/creatinina (P/C) en orina esporádica como alternativa diagnóstica, razón por la cual el objetivo del presente estudio fue determinar si el cociente P/C se correlaciona con la proteinuria en 24 horas (ProtU24h), en pacientes con ERC que asistieron al Centro UNILIME UC, Valencia-Venezuela. El estudio fue correlacional, de campo y transversal. La muestra fue de tipo intencional, no probabilística, constituida por 120 pacientes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Proteinuria; Depuración de creatinina; Índice proteína/creatinina; Orina esporádica.
Ano: 2015 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572015000200005
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Effect of castration on renal glycosaminoglycans and their urinary excretion in male and female rats with chronic renal failure BJMBR
Lemos,C.C.S.; Tovar,A.M.F.; Guimarães,M.A.M.; Bregman,R..
Glycosaminoglycans (GAGs) participate in a variety of processes in the kidney, and evidence suggests that gender-related hormones participate in renal function. The aim of this study was to analyze the relationship of GAGs, gender, and proteinuria in male and female rats with chronic renal failure (CRF). GAGs were analyzed in total kidney tissue and 24-h urine of castrated (c), male (M), and female (F) Wistar control (C) rats (CM, CMc, CF, CFc) and after 30 days of CRF induced by 5/6 nephrectomy (CRFM, CRFMc, CRFF, CRFFc). Total GAG quantification and composition were determined using agarose and polyacrylamide gel electrophoresis, respectively. Renal GAGs were higher in CF compared to CM. CRFM presented an increase in renal GAGs, heparan sulfate (HS), and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Castration; Chronic renal failure; Gender; Glycosaminoglycans; Proteinuria.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700567
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Effects of dietary lipids on renal function of aged rats BJMBR
Valente Gamba,C.; Zeraib Caraviello,A.; Matsushita,A.; Alves,G.M.; Nunes da Silva,L.; Nascimento Gomes,G.; Zaladek Gil,F..
Normal aging is accompanied by renal functional and morphological deterioration and dietetic manipulation has been used to delay this age-related decline. We examined the effects of chronic administration of diets containing 5% lipid-enriched diet (LD, w/w) on renal function of rats at different ages. Three types of LD were tested: canola oil, fish oil and butter. Mean systemic tail-cuff blood pressure and glycemia remained within the normal range whatever the age and the diet of the animals. Proteinuria began to rise from the 8th month in the groups ingesting LD, while in the control group it increased significantly (above 10 mg/24 h) only after the 10th month. With age, a significant and progressive decline in glomerular filtration rate (GFR) and renal...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Aging; Lipids; Proteinuria; Renal function; Acid excretion; Canola oil; Fish oil.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200016
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Evaluación del perfil tubular renal mediante electroforesis en gel de poliacrilamida ABCL
Facio,María Laura; Madalena,Leticia Bibiana; Bresciani,Pablo Diego; Pandolfo,Marcela; Kairúz,Aldana; Alejandre,Mariel Emilce; Fraind,Susana; Angerosa,Margarita; Pizzolato,Marco.
La electroforesis en gel de poliacrilamida con dodecil sulfato de sodio (SDS-PAGE) es un método de alta resolución para la tipificación de los perfiles proteicos urinarios (PPU), pero no ha sido rutinariamente adoptado en el laboratorio clínico. Se compararon los PPU mediante uroproteinograma electroforético (URO) y SDS-PAGE, ambos coloreados con tinción argéntica y la medición por turbidimetría de las proteínas de bajo peso molecular: a1-microglobulina (A1m) y b2-microglobulina (B2m). El objetivo del estudio fue establecer la sensibilidad de la coloración argéntica en SDS-PAGE para poder utilizarla principalmente en la definición de proteinuria tubular. Se procesaron 105 muestras de orina de 24 h de pacientes adultos que ingresaron al laboratorio con...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Electroforesis en gel de poliacrilamida - dodecil sulfato de sodio; Alfa-1 microglobulina; Beta-2 microglobulina; Daño tubular; Proteinuria; Uroproteinograma.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572006000300011
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Gross proteinuria is a strong risk predictor for cardiovascular mortality in Brazilian type 2 diabetic patients BJMBR
Cardoso,C.R.L.; Salles,G.F..
Increased proteinuria is recognized as a risk predictor for all-cause and cardiovascular mortality in diabetic patients; however, no study has evaluated these relationships in Brazilian patients. The aim of this study was to investigate the prognostic value of gross proteinuria for all-cause and cardiovascular mortalities and for cardiovascular morbidity in a cohort study of 471 type 2 diabetic individuals followed for up to 7 years. Several clinical, laboratory and electrocardiographic variables were obtained at baseline. The relative risks for all-cause, cardiovascular and cardiac mortalities and for cardiovascular and cardiac events associated with the presence of overt proteinuria (>0.5 g/24 h) were assessed by Kaplan-Meier survival curves and by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: All-cause mortality; Cardiovascular mortality; Cardiovascular morbidity; Proteinuria; Type 2 diabetes.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800006
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Multiple facets of HIV-associated renal disease BJMBR
da Silva,D.R.; Gluz,I.C.; Kurz,J.; Thomé,G.G.; Zancan,R.; Bringhenti,R.N.; Schaefer,P.G.; dos Santos,M.; Barros,E.J.G.; Veronese,F.V..
HIV infection has a broad spectrum of renal manifestations. This study examined the clinical and histological manifestations of HIV-associated renal disease, and predictors of renal outcomes. Sixty-one (64% male, mean age 45 years) HIV patients were retrospectively evaluated. Clinical presentation and renal histopathology were assessed, as well as CD4 T-cell count and viral load. The predictive value of histological lesion, baseline CD4 cell count and viral load for end-stage renal disease (ESRD) or death were determined using the Cox regression model. The outcomes of chronic kidney disease (CKD) and ESRD or death were evaluated by baseline CD4 cell count. The percent distribution at initial clinical presentation was non-nephrotic proteinuria (54%), acute...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HIV; Renal disease; Proteinuria; Collapsing focal segmental glomerulosclerosis; CD4 cell count; Chronic kidney disease.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400709
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Prevalence of persistent proteinuria in stable HIV/AIDS patients and its association with HIV nephropathy BJID
Cavalcante,Maria Alina Gomes de Mattos; Coelho,Sandra Neiva; Lacerda,Heloísa Ramos.
Human immunodeficiency virus (HIV)-related glomerular disease is a cause of end-stage renal disease, though there is no recent data from Brazil concerning this syndrome. Persistent proteinuria (PPt) is the main marker for glomerular disease, especially levels above 1.5 g. We examined the prevalence of and associated risk factors for PPt, along with the prevalence of HIV-associated nephropathy (HIVAN) among AIDS patients. We interviewed 411 patients who were attended at the HIV/AIDS section of the Clinical Hospital of the Federal University of Pernambuco (Brazil) from January through June 2004. PPt was defined as a positive urine dipstick exam on at least two occasions. The analyzed risk factors were: black race, a low CD4 lymphocyte count (<200...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Proteinuria; HIV nephropathy; AIDS.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500003
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Proteinuria in Malaria Patients in Minna, Nigeria OAK
Ogbadoyi, Emmanuel O.; Tembeng, Fon Charles.
Palavras-chave: Plasmodia; Proteinuria; Malaria; Renal failure.
Ano: 1999 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/316
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Proteinuria: lesión estructural renaly comparación de métodos ABCL
Madalena,Leticia; Pandolfo,Marcela; Facio,María Laura; Garlatti,Claudia; Alejandre¹,Mariel; Bresciani,Pablo Diego; Angerosa,Margarita.
La proteinuria (PT-ur) es un marcador de enfermedad renal, sin un método de referencia establecido. Mediante electroforesis en gel de poliacrilamida con dodecilsulfato de sodio (SDS-PAGE) con tinción argéntica pueden identificarse de manera no-invasiva perfiles proteicos urinarios (PPU) de tipo glomerular (G), tubular (Tc: completos, Ti: incompletos) y mixtos (GTc, GTi) con fines diagnósticos/pronósticos y de evolución. El objetivo del estudio fue determinar la prevalencia (P) de los PPU para cada nivel de PT-ur obtenido con cuatro métodos diferentes, en pacientes con sospecha de lesión renal. Se procesaron 105 orinas de 24 h de recolección clasificadas mediante tira reactiva (TR) y 2 pacientes con proteinuria de Bence Jones (PBJ)>2 g/L. La...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enfermedad renal; Proteinuria; Gel de poliacrilamida con dodecilsulfato de sodio; Proteína de Bence Jones; Cloruro de benzetonio.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572013000100011
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Raising awareness of chronic kidney disease in a Brazilian urban population BJMBR
Mazza Nascimento,M.; Riella,M.C..
The incidence and prevalence of chronic kidney disease have been increasing in recent years in developing countries. The aim of this study was to report the results of a general chronic kidney disease awareness program applied to an urban population in a large Brazilian city. From January 2002 to January 2005 a total of 8883 individuals in the city of Curitiba (PR, Brazil) were screened for hypertension, body mass index, hematuria, and proteinuria. A family history and previous medical diagnosis of hypertension and diabetes mellitus (DM) were also recorded. Of the 8883 individuals assessed, 56% were women, subject median age was 47 years (range: 17-93 years) and more than 90% were Caucasian. Thirty percent had signs of hematuria, 6% had proteinuria, and 3%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hematuria; Proteinuria; Prevention; Chronic kidney disease; Screening.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800010
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Transforming growth factor beta activity in urine of patients with type 2 diabetes and diabetic nephropathy BJMBR
Rivarola,E.W.R.; Moyses-Neto,M.; Dantas,M.; da-Silva,C.G.; Volpini,R.; Coimbra,T.M..
Diabetic nephropathy (DN) is characterized structurally by progressive mesangial deposition of extracellular matrix (ECM). Transforming growth factor-ß (TGF-ß) is considered to be one of the major cytokines involved in the regulation of ECM synthesis and degradation. Several studies suggest that an increase in urinary TGF-ß levels may reflect an enhanced production of this polypeptide by the kidney cells. We evaluated TGF-ß in occasional urine samples from 14 normal individuals and 23 patients with type 2 diabetes (13 with persistent proteinuria &gt;500 mg/24 h, DN, 6 with microalbuminuria, DMMA, and 4 with normal urinary albumin excretion, DMN) by enzyme immunoassay. An increase in the rate of urinary TGF-ß excretion (pg/mg UCreat.) was observed in...
Tipo: Info:eu-repo/semantics/other Palavras-chave: TGF-ß urinary excretion; Type 2 diabetes; Proteinuria; Diabetic nephropathy; Extracellular matrix.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200011
Registros recuperados: 13
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