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Genotypic characterization of psittacid herpesvirus isolates from Brazil BJM
Luppi,Marcela Miranda; Luiz,Ana Paula Moreira Franco; Coelho,Fabiana Magalhães; Ecco,Roselene; Fonseca,Flávio Guimarães da; Resende,Mauricio.
Abstract Thirty-six isolates of psittacid herpesvirus (PsHV), obtained from 12 different species of psittacids in Brazil, were genotypically characterized by restriction fragment length polymorphism (RFLP) analysis and PCR amplification. RFLP analysis with the PstI enzyme revealed four distinct restriction patterns (A1, X, W and Y), of which only A1 (corresponding to PsHV-1) had previously been described. To study PCR amplification patterns, six pairs of primers were used. Using this method, six variants were identified, of which, variants 10, 8, and 9 (in this order) were most prevalent, followed by variants 1, 4, and 5. It was not possible to correlate the PCR and RFLP patterns. Twenty-nine of the 36 isolates were shown to contain a 419 bp fragment of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Psittacid herpesvirus; PstI; RFLP; PCR.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100217
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