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| Garcia,Fabio Ribeiro; Cavalcante,Felipe Lira de Sá; Souza,Robson Antonio de; Houllou,Laureen Michelle. |
| ABSTRACT: One of the significant obstacles to the growth of sugarcane production is the infection by phytopathogens, mainly by the bacterium Leifsonia xyli subsp. xyli (Lxx) causal agent of Ratoon stunting disease. Thus, this research aimed to evaluate the effects of kasugamycin on the in vitro growth of sugarcane, as well as its effect on the bacterium Lxx. Explants of strain SP791011 from sugarcane were inoculated in MS culture medium supplemented with the antimicrobial kasugamycin at concentrations of 0.00; 0.87; 1.08; 1.74 and 3.48 mL.L-1, where they remained for 30 days. After this period, the survival rate, shoot number per explant, height of the explants, phytomass, dry phytomass and phytosanitary were evaluated based on the presence of genomic DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micropropagation; Endophytic; Ratoon stunting disease. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800404 |
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| SAWAZAKI, H. E.; SA, L. A. N. de; GONÇALVES, C. R. N. C. B.; VEIGA, R. F. A.; COLOMBO, C. A.. |
| Abstract: Aiming at optimizing the diagnosis of major sugarcane diseases by PCR, primers were developed (scald, orange rust) and amplified fragments according to the literature were used as positive control, such as those caused by: 1 - Bacteria, (ratoon stunting and leaf scald) 2 - Viruses, (yellow leaf, mosaic, mosaic streak and fijivirus) 3-Fungus, (smut, orange rust and curvularia). For diseases with long latency period (leaf scald, ratoon stunting, yellow leaf, mosaic, fijivirus, smut and orange rust), primers were designed for real time PCR. For testing, infected leaves, fungal colonies, amplified DNA fragment or 40 seedlings were used. Real time PCR analyses have enabled the detection of sugarcane samples with highest dilution of DNA. The pair of... |
| Tipo: Separatas |
Palavras-chave: Leaf scald; PCR; Cana de açúcar; Doença de planta; Diagnóstico; Bactéria; Vírus; Fungo; Escaldadura; Ferrugem alaranjada; Plant diseases and disorders; Sugarcane; Ratoon stunting disease; Scald diseases; Sugarcane yellow leaf virus; Sugarcane streak virus; Fijivirus; Smut diseases; Rust diseases; Curvularia; Polymerase chain reaction. |
| Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/963146 |
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