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The 20-hydroxyecdysone-induced signalling pathway in G2/M arrest of Plodia interpunctella imaginal wing cells Inra
Siaussat, D.; Bozzolan, F.; Porcheron, P.; Debernard, S..
The mechanisms involved in the control of cellular proliferation by the steroid hormone 20-hydroxyecdysone (20E) in insects are not known. We dissected the 20E signalling pathway responsible for G2/M arrest of imaginal cells from the IAL-PID2 cells of the Indian meal moth Plodia interpunctella. We first used a 5′–3′ RACE-based strategy to clone a 4479 bp cDNA encoding a putative P. interpunctella HR3 transcription factor named PiHR3. The deduced amino acid sequence of PiHR3 was highly similar to those of HR3 proteins from other lepidopterans, e.g. Manduca sexta and Bombyx mori. Using double-stranded RNA-mediated interference (dsRNAi), we then succeeded in blocking the ability of 20E to induce the expression of PiEcR-B1, PiUSP-2 and PiHR3 genes that encode...
Tipo: Journal Article Palavras-chave: PLODIA INTERPUNCTELLA; EUCARYOTE; ARTHROPODA; HEXAPODA; INSECTE; LEPIDOPTERA; HORMONE STEROIDE; MITE; CLONAGE; ADNC; EXPRESSION DES GENES; TRANSCRIPTION; SEQUENCE NUCLEOTIDIQUE; ACIDE AMINE  RNA INTERFERENCE; STEROID HORMONE RECEPTOR SUPERFAMILY; G2/M ARREST; B CYCLIN; SIGNALLING PATHWAY; IMAGINAL CELLS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20103c688c9f&uri=/notices/prodinra1/2010/10/
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