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Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny. Repositório Alice
MARTINS, F. A.; CARNEIRO, P. C. S.; CRUZ, C. D.; CARNEIRO, J. E. S. de; GUIMARÃES, C. T..
Abstract ? The objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Allelic origin; Natural populations; Polygenes; Semiquantitative PCR; Zea mays; Heterozygote.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/661195
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Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny PAB
Martins,Francielle Alline; Carneiro,Pedro Crescêncio Souza; Cruz,Cosme Damião; Carneiro,José Eustáquio de Souza; Guimarães,Claudia Teixeira.
The objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Allelic origin; Heterozygote; Natural populations; Polygenes; Semiquantitative PCR.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001000012
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Temporal expression of the sor1 gene and inhibitory effects of Sorghum bicolor L. Moench on three weed species Acta Botanica
Santos,Roseane Cavalcanti dos; Ferraz,Gabriela de Morais Guerra; Albuquerque,Manoel Bandeira de; Lima,Liziane Maria de; Melo Filho,Péricles de Albuquerque; Ramos,Alessandra de Rezende.
The temporal expression of gene sor1 and the inhibitory effect of Sorghum bicolor L. Moench against weeds were studied by semiquantitative polymerase chain reaction and intercropping management, respectively. To quantify sor1 expression, seeds were sown in pots and RNA was collected from the roots at 5, 10, 15, 20 and 30 days after emergence (DAE). In the inhibition assay, cotton and three weeds were evaluated during single cropping and during intercropping with S. bicolor. The assay was completely randomized, with eight replications. We found early expression of sor1 in most S. bicolor accessions by 5 DAE, and a gradual reduction thereafter. Only one of the accessions showed sor1 expression up to 30 DAE. In the inhibition assay, the most significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allelopathy; Semiquantitative PCR; Sorgoleone; Weeds.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000300008
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