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Monteiro,G.A.; Guasti,P.N.; Hartwig,F.P.; Dellaqua Jr.,J.A.; Alvarenga,M.A.; Papa,F.O.. |
After a serious injury or sudden death, epididymis cauda sperm recovery and cryopreservation may present as the last opportunity to obtain genetic material from a valuable stallion. This study evaluated the viability of cooled equine sperm collected by three different methods: sperm of ejaculated (G1), sperm recovered from the epididymal cauda immediately after orchiectomy (G2) and sperm recovered from the epididymal cauda after storage for 24 hours at 5°C (G3). To obtain G1 sperm, two ejaculates were collected. After 1 week, all stallions underwent a bilateral orchiectomy, and one of the removed epididymides was flushed to obtain G2 sperm. The contralateral epididymis was stored at 5°C for 24 hours before being flushed to obtain G3 sperm. The sperm... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Horse epididymal spermatozoa; Cryopreservation; Stallion; Sperm viability; Seminal plasma. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000300010 |
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