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A microbiological and biogeochemical investigation of the cold seep tubeworm Escarpia southwardae (Annelida: Siboglinidae): Symbiosis and trace element composition of the tube ArchiMer
Duperron, Sebastien; Gaudron, Sylvie M.; Lemaitre, Nolwenn; Bayon, Germain.
Tubeworms within the annelid family Siboglinidae rely on sulfur-oxidizing autotrophic bacterial symbionts for their nutrition, and are among the dominant metazoans occurring at deep-sea hydrocarbon seeps. Contrary to their relatives from hydrothermal vents, sulfide uptake for symbionts occurs within the anoxic subsurface sediment, in the posterior ‘root’ region of the animal. This study reports on an integrated microbiological and geochemical investigation of the cold seep tubeworm Escarpia southwardae collected at the Regab pockmark (Gulf of Guinea). Our aim was to further constrain the links between the animal and its symbiotic bacteria, and their environment. We show that E. southwardae harbors abundant sulfur-oxidizing bacterial symbionts in its...
Tipo: Text Palavras-chave: Sulfur-oxidizing bacteria; RTCA; Trace elements; RubisCO; Symbiosis.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00191/30251/28683.pdf
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A preliminary review of zoanthid-hermit crab symbioses (Cnidaria; Zoantharia/Crustacea, Paguridea) Naturalis
Ates, R.M.L..
A preliminary review is presented of zoanthid-hermit crab symbioses reported in the literature. The records of Balss (1924) are included. Aspects of this type of symbiosis are discussed.
Tipo: Article / Letter to the editor Palavras-chave: Hermit crabs; Paguridea; Colonial anemones; Zoantharia; Symbiosis; Review; 42.74.
Ano: 2003 URL: http://www.repository.naturalis.nl/record/220292
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A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots Genet. Mol. Biol.
Rodrigues,Elisete Pains; Torres,Adalgisa Ribeiro; Batista,Jesiane Stefânia da Silva; Huergo,Luciano; Hungria,Mariangela.
Sample preparation is a critical step in two-dimensional gel electrophoresis (2-DE) of plant tissues. Here we describe a phenol/SDS procedure that, although greatly simplified, produced well-resolved and reproducible 2-DE profiles of protein extracts from soybean [Glycine max (L.) Merril] roots. Extractions were made in three replicates using both the original and simplified procedure. To evaluate the quality of the extracted proteins, ten spots were randomly selected and identified by mass spectrometry (MS). The 2-DE gels were equally well resolved, with no streaks or smears, and no significant differences were observed in protein yield, reproducibility, resolution or number of spots. Mass spectra of the ten selected spots were compared with database...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-DE; Protein extraction; Proteomics; Soybean roots; Symbiosis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200016
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A study of chlorophyll-like and phycobilin pigments in the C endosymbiont of the apple- snail Pomacea canaliculata Biocell
Vega,Israel A.; Dellagnola,Federico A.; Hurst,Jorge A.; Godoy,Martín S.; Castro-Vazquez,Alfredo.
Pigments present in the brown-greenish C morph of an intracellular endosymbiont of Pomacea canaliculata were investigated. Acetone extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of chlorophylls. Three fractions obtained from silica gel column chromatography of the acetone extracts (C I, C II and C III), were studied by positive ion fast atom bombardment-mass spectrometry (FAB-MS) and hydrogen-nuclear magnetic resonance (H-NMR). Results indicated the presence of (1) a sterol in the yellow colored C I fraction; (2) a mixture of pheophorbides a and b in the major green fraction, C II; and (3) a modified pheophorbide a in the smaller green fraction, C III. Aqueous extracts of the C endosymbiont did not show evidence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Symbiosis; Cyanobacteria; Chloroplast; Photosynthetic pigments; Phylogeny.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200001
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A tale of two chitons: is habitat specialisation linked to distinct associated bacterial communities? ArchiMer
Duperron, Sebastien; Pottier, Marie-anne; Leger, Nelly; Gaudron, Sylvie M.; Puillandre, Nicolas; Le Prieur, Stephanie; Sigwart, Julia D.; Ravaux, Juliette; Zbinden, Magali.
Although most chitons (Mollusca: Polyplacophora) are shallow-water molluscs, diverse species also occur in deep-sea habitats. We investigated the feeding strategies of two species, Leptochiton boucheti and Nierstraszella lineata, recovered on sunken wood sampled in the western Pacific, close to the Vanuatu Islands. The two species display distinctly different associations with bacterial partners. Leptochiton boucheti harbours Mollicutes in regions of its gut epithelium and has no abundant bacterium associated with its gill. Nierstraszella lineata displays no dense gut-associated bacteria, but harbours bacterial filaments attached to its gill epithelium, related to the Deltaproteobacteria symbionts found in gills of the wood-eating limpet Pectinodonta sp....
Tipo: Text Palavras-chave: Sunken wood; Polyplacophora; Symbiosis; Mollicutes; Deltaproteobacteria; Deep-sea ecology.
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00138/24933/23035.pdf
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Acquisition of epibiotic bacteria along the life cycle of the hydrothermal shrimp Rimicaris exoculata ArchiMer
Guri, Mathieu; Durand, Lucile; Cueff-gauchard, Valerie; Zbinden, Magali; Crassous, Philippe; Shillito, Bruce; Cambon-bonavita, Marie-anne.
The caridean shrimp Rimicaris exoculata dominates the fauna at several Mid-Atlantic Ridge hydrothermal vent sites. This shrimp has an enlarged gill chamber, harboring a dense ectosymbiotic community of chemoautotrophic bacteria associated with mineral oxide deposits. Until now, their acquisition is not fully understood. At three hydrothermal vent sites, we analyzed the epibionts diversity at different moult stages and also in the first stages of the shrimp life (eggs, hatched eggs (with larvae) and juveniles). Hatched eggs associated with young larvae were collected for the first time directly from gravid females at the Logachev vent site during the Serpentine cruise. An approach using 16S rRNA clone libraries, scanning and transmission electron...
Tipo: Text Palavras-chave: Symbiosis; Larvae; Methanotrophic symbiont; Rimicaris exoculata; Transmission pathways.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00072/18318/15997.pdf
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Adaptations of lichens to conditions in tropical forests of South-East Asia and their taxonomic implications Naturalis
Wolseley, P.A.; Hawksworth, D.L..
Lichens are fungi with a specialized nutritional mode involving algae, or cyanobacteria, or both. Classification is based on the fungal partner, and around 13 500 species are known. The association is ancient, and the first ascomycete fungi with fruit bodies may have been lichenized. Adaptations to tropical habitats include extensive utilization of trentepohlioid algae, the production of large multi-celled spores capable of forming numerous germ tubes, and water-repellant hydophobins coating internal cell walls. Many tropical groups lack modern monographs and numerous new species are discovered in detailed studies. Lichens merit more attention in the tropics as bioindicators of habitat disturbance.
Tipo: Article / Letter to the editor Palavras-chave: Algae; Ascomycota; Bioindication; Coevolution; Symbiosis.
Ano: 2009 URL: http://www.repository.naturalis.nl/record/525176
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Antimicrobial Peptides and Ectosymbiotic Relationships: Involvement of a Novel Type IIa Crustin in the Life Cycle of a Deep-Sea Vent Shrimp ArchiMer
Le Bloa, Simon; Boidin-wichlacz, Céline; Cueff-gauchard, Valerie; Rosa, Rafael Diego; Cuvillier-hot, Virginie; Durand, Lucile; Methou, Pierre; Pradillon, Florence; Cambon-bonavita, Marie-anne; Tasiemski, Aurélie.
The symbiotic shrimp Rimicaris exoculata dominates the macrofauna inhabiting the active smokers of the deep-sea mid Atlantic ridge vent fields. We investigated the nature of the host mechanisms controlling the vital and highly specialized ectosymbiotic community confined into its cephalothoracic cavity. R. exoculata belongs to the Pleocyemata, crustacean brooding eggs, usually producing Type I crustins. Unexpectedly, a novel anti-Gram-positive type II crustin was molecularly identified in R. exoculata. Re-crustin is mainly produced by the appendages and the inner surfaces of the cephalothoracic cavity, embedding target epibionts. Symbiosis acquisition and regulating mechanisms are still poorly understood. Yet, symbiotic communities were identified at...
Tipo: Text Palavras-chave: Extreme; Hydrothermal; Symbiosis; Host-microbe interaction; Invertebrate immunity; Crustacean.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00643/75534/76413.pdf
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Approche métagénomique de la chimiosynthèse en système hydrothermal océanique profond : cas particulier des symbioses bactériennes associées à la crevette Rimicaris exoculata ArchiMer
Jan, Cyrielle.
The hydrothermal shrimp Rimicaris exoculata is an endemic species of the Mid-Atlantic Ridge (MAR).This species lives in dense aggregates on the walls of active chimneys and dominates the megafauna of most of these sites. This shrimp has two distinct microbial communities whose roles remain almost unknown. One of these communities is associated with the cephalothorax and hypertrophied mouthparts.These are covered by a dense community of bacteria affiliated to chemoautotrophic epibionts associated with mineral deposits. To deepen our knowledge on the symbiotic community in the context of the thesis, a metagenomic approach was undertaken. This helped highlighting the microbial diversity present with a dominant phylum, the Proteobacteria. Two classes are...
Tipo: Text Palavras-chave: Epibiontes; Métagénomique; Rimicaris exoculata; Symbiose; Zetaproteobacteria; Symbiosis; Epibionts; Metagenomic.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00498/60954/64357.pdf
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Arbuscular mycorrhiza in species of Commelinidae (Liliopsida) in the state of Pernambuco (Brazil) Acta Botanica
Silva,Gladstone Alves da; Santos,Bartolomeu Acioli dos; Alves,Marccus Vinícius; Maia,Leonor Costa.
Mycorrhiza are a mutualistic symbiosis between fungi and plant roots, the main benefit to the plant being increased nutrient uptake. The arbuscular is the most important kind of mycorrhiza for agriculture and it is widespread in occurrence and distribution in most ecosystems. The aim of this work was to study the mycorrhizal status of the species of Commelinidae that occur in the State of Pernambuco. Plant roots, collected in ten municipalities, were washed, cleared in KOH, stained with Trypan blue in lactoglycerol and observed under a light microscope in order to assess presence and identification of the mycorrhizal type. Percentage of root colonization was evaluated by the gridline intersect method. Forty specimens representing 30 species were observed....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyperaceae; Eriocaulaceae; Poaceae; Mycorrhizal fungi; Symbiosis.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062001000200002
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Association between deep-water scale-worms (Annelida: Polynoidae) and black corals (Cnidaria: Antipatharia) in the Southwestern Atlantic Rev. Bras. Zool.
De Assis,José Eriberto; Souza,José Roberto B. de; Lima,Manuela M. de; Lima,Gislaine V. de; Cordeiro,Ralf T.S.; Pérez,Carlos D..
ABSTRACT Polynoid scale-worms have been found living as commensals with deep-water antipatharians (commonly known as black corals) in the Potiguar Basin, off Rio Grande do Norte State, Northeastern Brazil. In this paper two polychaete species and four black corals species are redescribed. Benhamipolynoe cf. antipathicola and Parahololepidella cf. greeffi, and the black coral Stylopathes adinocrada Opresko, 2006 are recorded for the Southwestern Atlantic. Benhamipolynoe cf. antipathicola was first described from off New Zealand and the Malay Archipelago, as symbiont with the black coral Stylopathes tenuispina (Silberfeld, 1909). It was later reported for the North Atlantic, off Florida, associated with Stylopathes columnaris (Duchassaing, 1870). In our...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazil; Hexacorallia; Polynoids; South America; Symbiosis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702019000100315
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Bacterial communities associated with the wood-feeding gastropod Pectinodonta sp (Patellogastropoda, Mollusca) ArchiMer
Zbinden, Magali; Pailleret, Marie; Ravaux, Juliette; Gaudron, Sylvie M.; Hoyoux, Caroline; Lambourdiere, Josie; Waren, Anders; Lorion, Julien; Halary, Sebastien; Duperron, Sebastien.
Even though their occurrence was reported a long time ago, sunken wood ecosystems at the deep-sea floor have only recently received specific attention. Accumulations of wood fragments in the deep sea create niches for a diverse fauna, but the significance of the wood itself as a food source remains to be evaluated. Pectinodonta sp. is a patellogastropod that exclusively occurs on woody substrates, where individuals excavate deep depressions, and is thus a potential candidate for a wood-eating lifestyle. Several approaches were used on Pectinodonta sampled close to Tongoa island (Vanuatu) to investigate its dietary habits. Host carbon is most likely derived from the wood material based on stable isotopes analyses, and high cellulase activity was measured in...
Tipo: Text Palavras-chave: Sunken woods; Wood-feeding gastropod; Symbiosis; Cellulolytic activity.
Ano: 2010 URL: https://archimer.ifremer.fr/doc/00206/31687/30070.pdf
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Benefícios da adubação verde sobre a simbiose micorrízica e a produtividade de batata-doce. Infoteca-e
ESPÍNDOLA, J. A. A.; ALMEIDA, D. L. de; GUERRA, J. G. M..
Plantio das leguminosas e da batata doce. Produtividade da batata doce e simbiose micorrízica.
Tipo: Folhetos Palavras-chave: Ipomoea batata; Mycorrhiza; Production; Adubação Verde; Batata Doce; Legume; Leguminosa; Micorriza; Produção; Plantio; Simbiose; Green manures; Mycorrhizae; Symbiosis; Sweet potatoes.
Ano: 1997 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/623563
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"Beta-rizóbios": os novos simbiontes encontrados em espécies de Mimosa. Infoteca-e
REIS JÚNIOR, F. B. dos; FARIA, S. M. de; MENDES, I. de C.; SIMON, M. F.; LOUREIRO, M. de F.; ELLIOT, G. N.; YOUNG, P.; SPRENT, J. I.; JAMES, E. K..
The fixation and transformation of atmospheric dinitrogen into ammonia and hence into organic e compounds is a crucial part of the nitrogen cycle. Nitrogenase, the enzyme responsible, has a wide taxonomie distribution in the bacteria and archaea, but is confined to a limited number of species. Among these, rhizobia, whieh form nodules on the roots of legumes, are recognised as the main contributors of biologieally-fixed nitrogen. Until recently, it has been generally accepted that legumes are nodulated exelusively by members of the alfa-proteobaeteria belonging to a number of related genera in the family Rhizobiaceae. In this document we review some results from recent studies that presents the newly-discovered beta-rhizobia which challenge the accepted...
Tipo: Folhetos Palavras-chave: Feed legumes; Fixação de Nitrogênio; Leguminosa Forrageira; Simbiose; Rhizobium; Nitrogen fixation; Symbiosis.
Ano: 2006 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570271
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Bukholderia strains promote Mimosa spp. growth but not Macroptilium atropurpureum Rev. Ciênc. Agron.
Araújo,Kaliane Sírio; Carvalho,Fernanda de; Moreira,Fatima Maria de Souza.
ABSTRACT The aim of this study was to evaluate the relationship and symbiotic efficiency of 14 strains of Burkholderia isolated from rupestrian grasslands, using M. atropurpureum and Mimosa tenuiflora as trap plants, with the species M. atropurpureum, Mimosa bimucronata and M. foliolosa. For the nodulation and symbiotic efficiency test in M. atropurpureum, long-neck bottles containing nutrient solution were used. The experiments with Mimosa spp. were carried out in tubes containing vermiculite (160 cm3) and sand (80 cm3) (2:1). The parameters under evaluation were number of nodules, nodules dry matter production, shoots dry matter, roots dry matter, and total dry matter production for all the species analyzed; and plant height, diameter, and the Dickson...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rupestrian grasslands; Nitrogen-fixing bacteria; Symbiosis; Mimosa bimucronata; Mimosa foliolosa.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000100041
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Casuarina equisetifolia fitorremeciación de suelo contaminado con diesel y aplicación de bioestimulación y bioaumentación. Colegio de Postgraduados
Díaz Martínez, María Esther.
En este trabajo se evaluó la germinación de semillas y el crecimiento de plantas de C. equisetifolia L. ante diesel, y su potencial de fitorremediación en combinación de bioaumentación y bioestimulación. La primera fase experimental evaluó el efecto tóxico de 15 dosis de diesel (0, 100, 250, 500, 800, 1000, 1500, 2000, 2500, 3000, 5000, 7500, 10000, 15000 y 20000 mg kg-1) en la germinación de semillas y la emergencia de plántulas. A los 10 días, la germinación fue mayor en concentraciones altas de diesel; la mayor geminación ocurrió en 20000 mg kg-1, y la menor se registró ante 5000 mg kg-1. La emergencia de plántulas fue mayor en las concentraciones menores a 5000 mg kg-1. A los 15 días, el crecimiento de las plántulas disminuyó ante concentraciones...
Palavras-chave: Germinación; Emergencia de plantas; Simbiosis; Fertilización; Microorganismos; Degradación de hidrocarburos; Seed germination; Seedling emergence; Symbiosis; Fertilization; Microorganisms; Hydrocarbon degradation; Maestría; Edafología.
Ano: 2012 URL: http://hdl.handle.net/10521/730
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Characterization of seed germination and protocorm development of Cyrtopodium glutiniferum (Orchidaceae) promoted by mycorrhizal fungi Epulorhiza spp. Acta Botanica
Pereira,Marlon Corrêa; Rocha,Diego Ismael; Veloso,Tomás Gomes Reis; Pereira,Olinto Liparini; Francino,Dayana Maria Teodoro; Meira,Renata Maria Strozi Alves; Kasuya,Maria Catarina Megumi.
Cyrtopodium glutiniferum is an endemic orchid of Brazil with potential medicinal and ornamental applications. As mycorrhizal fungi are essential for the initiation of the orchid life cycle, the aim of this study was to determine the strains of mycorrhizal fungi suitable for seed germination and protocorm development of C. glutiniferum and to characterize the symbiotic development of protocorms. Seeds of C. glutiniferum were inoculated with nine mycorrhizal fungi, Epulorhiza spp., Ceratorhiza spp., Rhizoctonia sp., originally isolated from Brazilian neotropical orchids. Only Epulorhiza isolates promoted seed germination and protocorm development. Three Epulorhiza isolates (M1, M6 = E. epiphytica, M20 = Epulorhiza sp.) promoted protocorm development until...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ceratorhiza; Epulorhiza; Orchid conservation; Seedling production; Symbiosis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400567
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Crecimiento de cuatro cepas del género Rhizobium en presencia de hidrocarburos policíclicos aromáticos (HPA). Colegio de Postgraduados
González Paredes, Yessica.
Este estudio evaluó el crecimiento de cuatro cepas de Rhizobium en medio contaminado con fenantreno (PHE) o benzo[a]pireno (BaP) en concentraciones de 0, 10, 20, 40, 60, 80 y 100 µg mL-1. En la primera fase experimental, R. tropici CIAT899, R. tropici PRF81, R. leucaena CFN299 y R. etli CFN42 crecieron en condiciones in vitro o en simbiosis con Phaseolus vulgaris L. en presencia de PHE y BaP. El crecimiento in vitro de las cuatro cepas a las 24 h, disminuyó al aumentar las concentraciones de ambos HPA; sin embargo, el BaP fue más tóxico para su crecimiento. Las cuatro cepas ante 80 y 100 µg BaP mL-1 presentaron una fase de adaptación; para CIAT899 y PRF81 esta fase fue de 48 h, y para CFN299 y CFN42 esta fase fue de 72 y 96 h, respectivamente. La simbiosis...
Palavras-chave: Rizobios; Tolerancia; Adaptación; Simbiosis; Degradación; Fenantreno; Benzol [a] pireno; Rhizobia; Tolerance; Adaptation; Symbiosis; Degradation; Phenanthrene; Benzol [a] pyrene; Edafología; Maestría.
Ano: 2012 URL: http://hdl.handle.net/10521/1704
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Cyrtopodium paludicolum germination with two Tulasnella isolates Acta Botanica
Carvalho,Otieres Cirino de; Paiva Neto,Vespasiano Borges de; Padilha,Daly Roxana Castro; Veloso,Tomás Gomes Reis; Bocayuva,Melissa Faust; Soares,Deanna Carla Oliveira; Kasuya,Maria Catarina Megumi.
ABSTRACT Symbiosis between orchid seeds and mycorrhizal fungi has been reported to be a determining factor in the success of germination and protocorm development in vitro. The aim of this study was to isolate and identify by molecular analysis the mycorrhizal fungus associated with Cyrtopodium paludicolum, and to evaluate its efficiency in facilitating seed germination and development. Germination experiments were carried out using a fungus isolated from C. paludicolum (CH01) and Epidendrum secundum (M65), which has been successfully used a number of times in symbiotic germination. The experiments were conducted in a completely randomized design with treatments of CH01, M65 as well as under asymbiotic conditions. The mycobiont CH01 was successfully...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; ITS-DNAr; Mycorrhiza; Orchidaceae; Symbiosis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100107
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Description of two new associated infaunal decapod crustaceans (Axianassidae and Alpheidae) from the tropical eastern Pacific Pap. Avulsos de Zool. (São Paulo)
Anker,Arthur; Lazarus,Juan Felipe.
Two new species of infaunal decapod crustaceans are described based on material collected in Bahía Málaga, Pacific coast of Colombia, in 2009. The mud-shrimp Axianassa darrylfelderi sp. nov. (Axianassidae) appears to be most closely related to A. australis Rodrigues & Shimizu, 1992, A. canalis Kensley & Heard, 1990, and A. jamaicensis Kensley & Heard, 1990. The new species may be distinguished from each of them by a combination of morphological features, mainly on the uropodal exopod, antennal acicle, third maxilliped and first pleonite. The shrimp Leptalpheus canterakintzi sp. nov. (Alpheidae), associated with burrows of A. darrylfelderi sp. nov., undoubtedly represents the eastern Pacific sister species of the western Atlantic L. axianassae...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Decapoda; Shrimp; Mud-shrimp; Infauna; Symbiosis; Axianassidae; Alpheidae; Axianassa; Leptalpheus; Transisthmian species; East Pacific; New species; New record..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492015000800115
Registros recuperados: 75
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